• Korean Journal of Child Studies
• /
• v.11 no.1
• /
• pp.45-57
• /
• 1990

The purpose of the present study was to investigate the age-related differences in recall and to assess effects of presentation modality, encoding condition and cue modality on recall in terms of encoding specificity principles and dual-coding theory. Eighty children in each of 3grades(first, third and fifth) were presented a 30-item set of pictures or words on cars for recall in a study-test procedure. The experiment was designed as a 3(age) x 2(presentation modality:picture or word) x 2(encoding condition:random or category) x 2(cue modality:picuture or word) factorial design. Statistical analyses were with four-way ANOVA and $Scheff\acute{e}$ test. It was concluded from these results that when the stimulus was presented by pictures, encoded by category and the cues were also presented by pictures, recall increased in all ages. These results were interpreted in terms of encoding specificity principles and dual-coding theory.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.12
• /
• pp.2024-2033
• /
• 2015

A gene encoding lipolytic enzyme, lip7-3, was isolated from Bacillus sp. W130-35 isolated from a tidal mud flat. The gene encoded a protein of 215 amino acids with a signal peptide composed of 34 amino acid residues. Lip7-3 belonged to the family I.4 lipase and showed its maximal activity at pH 9.0 and 60℃. Its activity increased in the presence of 30% methanol and, remarkably, increased as well to 154.6% in the presence of Ca²⁺. Lip7-3 preferred p-nitrophenyl octanoate (C8) as a substrate and exhibited broad specificity for short- to long- chain fatty acid esters. Additionally, Lip7-3 showed a low degree of enantioselectivity for an S-enantiomer (e.g., (S)-methyl-3-hydroxy-2-methylpropionate). It efficiently hydrolyzed glyceryl tributyrate, but did not hydrolyze glyceryl trioleate, fish oil, or olive oil. Its substrate specificity and activation by the solvent might offer a merit to the biotechnological enzyme applications like transesterification in the production of biodiesel.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.1
• /
• pp.74-80
• /
• 2007

An enantioselective lipase gene (esf) for the kinetic resolution of optically active (S)-flurbiprofen was cloned from the new strain Serratia marcescens ES-2. The esf gene was composed of a 1,845-bp open reading frame encoding 614 amino acid residues with a calculated molecular mass of 64,978 Da. The lipase expressed in E. coli was purified by a three-step procedure, and it showed preferential substrate specificity toward the medium-chain-length fatty acids. The esf gene encoding the enantioselective lipase was reintroduced into the parent strain S. marcescens ES-2 for secretory overexpression. The transformant S. marcescens BESF secreted up to 217kU/ml of the enantioselective lipase, about 54-fold more than the parent strain, after supplementing 3.0% Triton X-207. The kinetic resolution of (S)-flurbiprofen was carried out even at an extremely high (R,S)-flurbiprofen ethyl ester [(R,S)-FEE] concentration of 500 mM, 130 kU of the S. marcescens ES-2 lipase per mmol of (R,S)-FEE, and 1,000 mM of succinyl ${\beta}-cyclodextrin$ as the dispenser at $37^{\circ}C$ for 12h, achieving the high enantiomeric excess and conversion yield of 98% and 48%, respectively.

• Korean Journal of Medicinal Crop Science
• /
• v.12 no.5
• /
• pp.424-427
• /
• 2004

Microsomal ${\omega}-3$ fatty acid desaturase (FAD3) is an essential enzyme in the production of the n-3 polyunsaturated fatty acid ${\alpha}-linolenic$ acid during the seed developing stage. To understand the regulatory mechanism of the gene encoding the ${\omega}-3$ fatty acid desaturase, a genomic fragment corresponding to the previously isolated perilla seed PfFAD3 cDNA was amplified from perilla (Perilla frutescens Britt) by GenomeWalker PCR. Sequence analysis of the fragment provided with identification of a 1485-bp 5'-upstream region and a 241-bp intron in the open reading frame. To determine the tissue-specificity of the PfFAD3 gene expression, the 5'-upstream region was fused to the ${\beta}-glucuronidase$ (GUS) gene and incorporated into Arabidopsis thaliana. Histochemical assay of the transgenic plants showed that GUS expression was restricted to seed and pollen, showing that PfFAD3 gene was exclusively expressed in those tissues.

• Journal of Microbiology and Biotechnology
• /
• v.23 no.3
• /
• pp.357-363
• /
• 2013

The identification of bacteriophage proteins on the surface of Lactobacillus rhamnosus Pen was performed by LC-MS/MS analysis. Among the identified proteins, we found a phage-derived major tail protein, two major head proteins, a portal protein, and a host specificity protein. Electron microscopy of a cell surface extract revealed the presence of phage particles in the analyzed samples. The partial sequence of genes encoding the major tail protein for all tested L. rhamnosus strains was determined with specific primers designed in this study. Next, RT-PCR analysis allowed detection of the expression of the major tail protein gene in L. rhamnosus strain Pen at all stages of bacterial growth. The transcription of genes encoding the major tail protein was also proved for other L. rhamnosus strains used in this study. The present work demonstrates the spontanous release of prophage-encoded particles by a commercial probiotic L. rhamnosus strain, which did not significantly affect the bacterial growth of the analyzed strain.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.17 no.5
• /
• pp.1396-1412
• /
• 2023

Conversation modeling is an important and challenging task in the field of natural language processing because it is a key component promoting the development of automated humanmachine conversation. Most recent research concerning conversation modeling focuses only on the current utterance (considered as the current question) to generate a response, and thus fails to capture the conversation's logic from its beginning. Some studies concatenate the current question with previous conversation sentences and use it as input for response generation. Another approach is to use an encoder to store all previous utterances. Each time a new question is encountered, the encoder is updated and used to generate the response. Our approach in this paper differs from previous studies in that we explicitly separate the encoding of the question from the encoding of its context. This results in different encoding models for the question and the context, capturing the specificity of each. In this way, we have access to the entire context when generating the response. To this end, we propose a deep neural network-based model, called the Context Model, to encode previous utterances' information and combine it with the current question. This approach satisfies the need for context information while keeping the different roles of the current question and its context separate while generating a response. We investigate two approaches for representing the context: Long short-term memory and Convolutional neural network. Experiments show that our Context Model outperforms a baseline model on both ConvAI2 Dataset and a collected dataset of conversational English.

• Proceedings of the IEEK Conference
• /
• 2001.06d
• /
• pp.203-206
• /
• 2001

This Paper suggests real-time video streaming method by using DCT-based scalability, and evaluates and analyzes the function. It is similar to using lowpass filter. That is, as following figure, this method is to split the encoded data in splitter and transmit it, and to decode the data according to the situation. This method can be applied to any video CODEC which is based on DCT. Therefore, this thesis suggests easy video streaming method by using DCT-based scalability, and shows the result of experiment. By using suggested scalability, calculations are reduced, and spacial scalability is realized. Moreover, the objective data which meet user's need according to the network condition and choose the appropriate scalability according to the capability of terming can be extracted. And it is possible to apply any resources according to the specificity of image.

• Journal of Microbiology and Biotechnology
• /
• v.13 no.1
• /
• pp.35-42
• /
• 2003

A 1,3 kb cellulase gene encoding novel bifunctional cellulase-chitosanase activity was cloned from biopolymer-producing alkali-tolerant B. lichenformis NBL420 in E. coli. A recombinant cellulase-chitosanase, named CelA, was expressed and purified to homogeneity. The activity staining and the enzymatic characterization of the purified CeIA revealed bifunctional activities on carboxymethyl cellulose (CMC) and glycol-chitosan. The similar characteristics of the enzymatic activities at the optimum pH, optimum temperature, and thermostability Indicated that CelA used a common catalytic domain with relaxed substrate specificity. A comparison of the deduced amino acids in the N-terminal region revealed that the mature CelA had a high homology with the previously identified bifunctional cellulase-chitosanase of Myxobacter sp. AL- 1.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.6
• /
• pp.845-855
• /
• 2015

The present study describes the gene cloning and high-level expression of an alkaline and thermostable lipase gene from Trichosporon coremiiforme V3. Nucleotide analysis revealed that this lipase gene has an open reading frame of 1,692 bp without any introns, encoding a protein of 563 amino acid residues. The lipase gene without its signal sequence was cloned into plasmid pPICZαA and overexpressed in Pichia pastoris X33. The maximum lipase activity of recombinant lipase was 5,000 U/ml, which was obtained in fed-batch cultivation after 168 h induction with methanol in a 50 L bioreactor. The purified lipase showed high temperature tolerance, and being stable at 60℃ and kept 45% enzyme activity after 1 h incubation at 70℃. The stability, effects of metal ions and other reagents were also determined. The chain length specificity of the recombinant lipase showed high activity toward triolein (C18:1) and tripalmitin (C16:0).

• Microbiology and Biotechnology Letters
• /
• v.22 no.6
• /
• pp.599-606
• /
• 1994

Bacillus stearothermomophilus, a strong xylan degrader, was confirmed to express multiple esterase activities in addition to the major xylanolytic enzymes. One of the genes encoding the esterases was isolated from the genomic library of B. stearothermophilus constructed with EcoRl restriction endonuclease and pBR322 plasmid. Three recombinant plasmids showing the tributyrin degrading activity were selected from approximately 7, 000 E. coli HB101 transformants, and were found to have the same insert of a 3.2 kb DNA fragment. Restriction mapping and hybridization studies revealed that the gene(estII) on the hybrid plasmid (pKMG7) had originated from the B. stearothermophilus chromosome, and was distinct from the estl, another esterase gene of B. stearothermophilus isolated in the previous work. The E. coli cells harboring pKMG7 produced an acetylxylan esterase that exibited similar substrate specificity to the esterase encoded by the estI gene.