• The Plant Pathology Journal
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• v.31 no.2
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• pp.195-201
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• 2015

Plant growth promoting rhizobacteria (PGPR) are known to confer disease resistance to plants. Bacillus sp. JS demonstrated antifungal activities against five fungal pathogens in in vitro assays. To verify whether the volatiles of Bacillus sp. JS confer disease resistance, tobacco leaves pre-treated with the volatiles were damaged by the fungal pathogen, Rhizoctonia solani and oomycete Phytophthora nicotianae. Pre-treated tobacco leaves had smaller lesion than the control plant leaves. In pathogenesis-related (PR) gene expression analysis, volatiles of Bacillus sp. JS caused the up-regulation of PR-2 encoding ${\beta}$-1,3-glucanase and acidic PR-3 encoding chitinase. Expression of acidic PR-4 encoding chitinase and acidic PR-9 encoding peroxidase increased gradually after exposure of the volatiles to Bacillus sp. JS. Basic PR-14 encoding lipid transfer protein was also increased. However, PR-1 genes, as markers of salicylic acid (SA) induced resistance, were not expressed. These results suggested that the volatiles of Bacillus sp. JS confer disease resistance against fungal and oomycete pathogens through PR genes expression.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.525-531
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• 2005

From a genomic library of Lactobacillus reuteri ATCC 55739, one clone, NE347, carrying a pyrH gene encoding UMP kinase, was identified. pNE347 carried a 1.88 kb EcoRI fragment and the pyrH was located in the middle of the insert. pyrH ORF was 723 bp in size and capable of encoding UMP kinase composed of 240 amino acid residues. tsf encoding an elongation factor-Ts and frr encoding a ribosomal recycling factor were present upstream and downstream of pyrH, respectively. When introduced into E. coli KUR1244, a pyrH-negative strain, pNE347 restored the ability to grow at $42^{\circ}C$, indicating that pyrH from L. reuteri synthesized functional UMP kinase in E. coli. Northern blot experiment showed that pyrH and frr were cotranscribed as a 1.4 kb single transcript. pyrH was overexpressed in E. coli by using a pET26b(+) vector, and a major 25 kDa protein band appeared on SDS-polyacrylamide gel.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.12B
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• pp.1548-1555
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• 2011

In ultra-deep submicron technology, minimization of propagation delay and power consumption on buses is one of the most important design objectives in system-on-chip (SOC) design. Crosstalk between adjacent wires on the bus may create a significant portion of propagation delay. Elimination or minimization of such faults is crucial to the performance and reliability of SOC designs. Most of the previous works on bus encoding are targeted either to minimize the bus switching or minimize the crosstalk delay, but not both. This paper proposes a new bus encoding scheme which can adaptively select one of functions "invert" and "logic-convert" according the number of bus switching on an encoded 4-bit cluster. This scheme leads to minimization of both crosstalk and bus switching. In experiment result, our proposed encoding technique consumes about 25% less power over the previous, while completely eliminating the crosstalk delay.

• Molecules and Cells
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• v.24 no.2
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• pp.215-223
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• 2007

The genes encoding non-specific lipid transfer proteins (nsLTPs), members of a small multigene family, show a complex pattern of expressional regulation, suggesting that some diversification may have resulted from changes in their expression after duplication. In this study, the evolution of nsLTP genes within the Poaceae family was characterized via a survey of the pseudogenes and unigenes encoding the nsLTP in rice pseudomolecules and the NCBI unigene database. nsLTP-rich regions were detected in the distal portions of rice chromosomes 11 and 12; these may have resulted from the most recent large segmental duplication in the rice genome. Two independent tandem duplications were shown to occur within the nsLTP-rich regions of rice. The genomic distribution of the nsLTP genes in the rice genome differs from that in wheat. This may be attributed to gene migration, chromosomal rearrangement, and/or differential gene loss. The genomic distribution pattern of nsLTP genes in the Poaceae family points to the existence of some differences among cereal nsLTP genes, all of which diverged from an ancient gene. The unigenes encoding nsLTPs in each cereal species are clustered into five groups. The somewhat different distribution of nsLTP-encoding EST clones between the groups across cereal species imply that independent duplication(s) followed by subfunctionalization (and/or neofunctionalization) of the nsLTP gene family in each species occurred during speciation.

• The KIPS Transactions:PartB
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• v.11B no.2
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• pp.177-186
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• 2004

It is a weak point of the motion estimation technique for video compression that the predicted video encoding algorithm requires higher-order computational complexity. To reduce the computational complexity of encoding algorithms, researchers introduced techniques such as 3D-WT that don't require motion prediction. One of the weakest points of previous 3D-WT studies is that they require too much memory for encoding and too long delay for decoding. In this paper, we propose a technique called `FS (Fast playable and Scalable) 3D-WT' This technique uses a modified Haar wavelet transform algorithm and employs improved encoding algorithm for lower memory and shorter delay requirement. We have executed some tests to compare performance of FS 3D-WT and 3D-V. FS 3D-WT has exhibited the same high compression rate and the same short processing delay as 3D-V has.

• Journal of Microbiology and Biotechnology
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• v.26 no.6
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• pp.1026-1034
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• 2016

In the current study, a total of 135 enterococci strains from different sources were screened for the presence of the enterocin-encoding genes entA, entP, entB, entL50A, and entL50B. The enterocin genes were present at different frequencies, with entA occurring the most frequently, followed by entP and entB; entL50A and L50B were not detected. The occurrence of single enterocin genes was higher than the occurrence of multiple enterocin gene combinations. The 80 isolates that harbor at least one enterocin-encoding gene (denoted "Gene⁺ strains") were screened for antimicrobial activity. A total of 82.5% of the Gene⁺ strains inhibited at least one of the indicator strains, and the isolates harboring multiple enterocin-encoding genes inhibited a larger number of indicator strains than isolates harboring a single gene. The indicator strains that exhibited growth inhibition included Listeria innocua strain CLIP 12612 (ATCC BAA-680), Listeria monocytogenes strain CDC 4555, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, S. aureus ATCC 29213, S. aureus ATCC 6538, Salmonella enteritidis ATCC 13076, Salmonella typhimurium strain UK-1 (ATCC 68169), and Escherichia coli BAC 49LT ETEC. Inhibition due to either bacteriophage lysis or cytolysin activity was excluded. The growth inhibition of antilisterial Gene⁺ strains was further tested under different culture conditions. Among the culture media formulations, the MRS agar medium supplemented with 2% (w/v) yeast extract was the best solidified medium for enterocin production. Our findings extend the current knowledge of enterocin-producing enterococci, which may have potential applications as biopreservatives in the food industry due to their capability of controlling food spoilage pathogens.

• Journal of Broadcast Engineering
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• v.24 no.6
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• pp.965-973
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• 2019

In this paper, Stereo 4K@60fps 360 VR real-time video capture system which consists of video stream capture, video encoding and stitching module is been designed. The system captures stereo 4K@60fps 360 VR video by stitching 6 of 2K@60fps stream which are captured through HDMI interface from 6 cameras in real-time. In video capture phase, video is captured from each camera using multi-thread in real-time. In video encoding phase, raw frame memory transmission and parallel encoding are used to reduce the resource usage in data transmission between video capture and video stitching modules. In video stitching phase, Real-time stitching is secured by stitching calibration preprocessing.

• Journal of Microbiology and Biotechnology
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• v.34 no.2
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• pp.280-288
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• 2024

The fusogenic membrane glycoprotein (FMG) derived from the human endogenous retrovirus-W (HERV-W) exhibits fusogenic properties, making it a promising candidate for cancer gene therapy. When cells are transfected with HERV-W FMG, they can fuse with neighboring cells expressing the receptor, resulting in the formation of syncytia. These syncytia eventually undergo cell death within a few days. In addition, it has been observed that an HERV-W env mutant, which is truncated after amino acid 483, displays increased fusogenicity compared to the wild-type HERV-W env. In this study, we observed syncytium formation upon transfection of HeLa and TE671 human cancer cells with plasmids containing the HERV-W 483 gene. To explore the potential of a semi-replication-competent retroviral (s-RCR) vector encoding HERV-W 483 for FMG-mediated cancer gene therapy, we developed two replication-defective retroviral vectors: a gag-pol vector encoding HERV-W 483 (MoMLV-HERV-W 483) and an env vector encoding VSV-G (pCLXSN-VSV-G-EGFP). When MoMLV-HERV-W 483 and pCLXSN-VSV-G-EGFP were co-transfected into HEK293T cells to produce the s-RCR vector, gradual syncytium formation was observed. However, the titers of the s-RCR virus remained consistently low. To enhance gene transfer efficiency, we constructed an RCR vector encoding HERV-W 483 (MoMLV-10A1-HERV-W 483), which demonstrated replication ability in HEK293T cells. Infection of A549 and HT1080 human cancer cell lines with this RCR vector induced syncytium formation and subsequent cell death. Consequently, both the s-RCR vector and RCR encoding HERV-W 483 hold promise as valuable tools for cancer gene therapy.

• Molecules and Cells
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• v.20 no.1
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• pp.112-118
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• 2005

It was previously shown that AtNAP1 is a plastidic SufB protein involved in Fe-S cluster assembly in Arabidopsis. In this study, we investigated the effects of depleting SufB protein from plant cells using virus-induced gene silencing (VIGS). VIGS of NbNAP1 encoding a Nicotiana benthamiana homolog of AtNAP1 resulted in a leaf yellowing phenotype. NbNAP1 was expressed ubiquitously in plant tissues with the highest level in roots. A GFP fusion protein of the N-terminal region (M1-V103) of NbNAP1 was targeted to chloroplasts. Depletion of NbNAP1 resulted in reduced numbers of chloroplasts of reduced size. Mitochondria also seemed to be affected. Despite the reduced number and size of the chloroplasts in the NbNAP1 VIGS lines, the expression of many nuclear genes encoding chloroplast-targeted proteins and chlorophyll biosynthesis genes remained unchanged.

• Proceedings of the Korean Information Science Society Conference
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• 1999.10b
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• pp.392-394
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• 1999

PSTN(Public Switch Telephone Network)에서 동영상을 전송하기 위해 H.263이라는 표준이 발표되었다. 저속의 전송률을 가지는 PSTN을 이용해서 영상회의나 영상전화 등을 구현하기 위해서는 기존의 코딩방식으로는 데이터를 전송하는데 문제점이 많았다. 이를 위해서 개발된 것이 H.263이다. H.263은 H.261에 기반을 두고 있으며 .261에 비해서 동일화질을 제공하는데 반정도의 데이터 양으로도 가능하게 해준다. 영상 압축 Encoder는 일반적으로 Decoder에 비하여 영상을 처리하는데 많은 시간이 소요된다. 그러나 VOD등과 같은 실시간으로 압축할 필요가 없는 경우에 대해서는 인코더가 많은 시간을 소비하더라고 큰 문제가 없는 반면에, 영상 회의나 영상 전화 등은 실시간 영상 Encoding, Decoding을 수행해야 한다. 그러기 위해서 고가의 하드웨어를 사용하게 된다. 이와 같은 이유에서 본 연구에서는 H.263을 소프트웨어만으로 Encoding 속도향상을 꾀하고자 하는 것이 이 논문의 목표이다.