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Silencing of NbNAP1 Encoding a Plastidic SufB-like Protein Affects Chloroplast Development in Nicotiana benthamiana  

Ahn, Chang Sook (Division of Bioscience and Bioinformatics, Myongji University)
Lee, Jeong Hee (Laboratory of Plant Genomics, Korea Research Institute of Bioscience and Biotechnology)
Pai, Hyun-Sook (Division of Bioscience and Bioinformatics, Myongji University)
Publication Information
Molecules and Cells / v.20, no.1, 2005 , pp. 112-118 More about this Journal
Abstract
It was previously shown that AtNAP1 is a plastidic SufB protein involved in Fe-S cluster assembly in Arabidopsis. In this study, we investigated the effects of depleting SufB protein from plant cells using virus-induced gene silencing (VIGS). VIGS of NbNAP1 encoding a Nicotiana benthamiana homolog of AtNAP1 resulted in a leaf yellowing phenotype. NbNAP1 was expressed ubiquitously in plant tissues with the highest level in roots. A GFP fusion protein of the N-terminal region (M1-V103) of NbNAP1 was targeted to chloroplasts. Depletion of NbNAP1 resulted in reduced numbers of chloroplasts of reduced size. Mitochondria also seemed to be affected. Despite the reduced number and size of the chloroplasts in the NbNAP1 VIGS lines, the expression of many nuclear genes encoding chloroplast-targeted proteins and chlorophyll biosynthesis genes remained unchanged.
Keywords
ABC Protein; Chloroplast Localization; Fe-S Cluster; NbNAP1; Plastid Factor; Virus-induced Gene Silencing;
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1 Austin, M. J., Muskett, P., Kahn, K., Feys, B. J., Jones, J. D., et al. (2002) Regulatory role of SGT1 in early R gene-mediated plant defenses. Science 295, 2077-2080   DOI   ScienceOn
2 Kushnir, S., Babiychuk, E., Storozhenko, S., Davey, M. W., Papenbrock, J., et al. (2001) A mutation of the mitochondrial ABC transporter Sta1 leads to dwarfism and chlorosis in the Arabidopsis mutant starik. Plant Cell 13, 89-100   DOI
3 Moller, S. G., Kunkel, T., and Chua, N.-H. (2001) A plastidic ABC protein involved in intercompartmental communication of light signaling. Genes Dev. 15, 90-103   DOI
4 Nachin, L., Loiseau, L., Expert, D., and Barras, F. (2003) SufC: an unorthodox cytoplasmic ABC/ATPase required for [Fe-S] biogenesis under oxidative stress. EMBO J. 22, 427-437   DOI   ScienceOn
5 Oelmuller, R. (1989) Photooxidative destruction of chloroplasts and its effect on nuclear gene expression and extraplastidic enzyme levels. Photochem. Photobiol. 49, 229-239   DOI
6 Susek, R., Ausubel, F. M., and Chory, J. (1993) Signal transduction mutants of Arabidopsis uncouple nuclear CAB and RBCS gene expression from chloroplast development. Cell 74, 787-799   DOI   ScienceOn
7 Cho, H. S., Lee, S. S., Kim, K. D., Kim, S. J., Hwang, I., et al. (2004) DNA gyrase is involved in chloroplast nucleoid partitioning. Plant Cell 16, 2665-2682   DOI   ScienceOn
8 Chen, X. and Schnell, D. J. (1999) Protein import into chloroplasts. Trends Cell Biol. 9, 222-227   DOI   ScienceOn
9 Kapazoglou, A., Mould, R. M., and Gray, J. C. (2000) Assembly of the Rieske iron-sulphur protein into the cytochrome bf complex in thylakoid membranes of isolated pea chloroplasts. Eur. J. Biochem. 267, 352-360   DOI   ScienceOn
10 Liu, Y., Schiff, M., Serino, G., Deng, X.-W., and Dinesh-Kumar, S. P. (2002) Role of SCF ubiquitin-ligase and the COP9 signalosome in the N gene-mediated resistance response to Tobacco mosaic virus. Plant Cell 14, 1483-1493   DOI
11 Osteryoung, K. W. and Nunnari, J. (2003) The division of endosymbiotic organelles. Science 302, 1698-1704   DOI   ScienceOn
12 Lee, S.-C., Huh, K.-Y., An, K., An, G., and Kim, S.-R. (2004) Ectopic expression of a cold-inducible transcription factor, CBF/DREB1b, in transgenic rice (Oryza sativa L.). Mol. Cells 18, 107-114
13 Ratcliff, F., Martin-Hernansez, A. M., and Baulcombe, D. C. (2001) Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J. 25, 237-245   DOI   ScienceOn
14 Takahashi, Y. and Tokumoto, U. (2002) A third bacterial system for the assembly of iron-sulfur clusters with homologs in archea and plastids. J. Biol. Chem. 277, 28380-28383   DOI   ScienceOn
15 Lee, S. S., Cho, H. S., Yoon, G. M., Ahn, J.-W., Kim, H. H., et al. (2003) Interaction of NtCDPK1 calcium-dependent protein kinase with NtRpn3 regulatory subunit of the 26S proteasome in Nicotiana tabacum. Plant J. 33, 825-840   DOI   ScienceOn
16 Sanchez-Fernandez, R., Davies, T. G. E., Coleman, J. O. D., and Rea, P. A. (2001) The Arabidopsis thaliana ABC protein superfamily, a complete inventory. J. Biol. Chem. 276, 30231- 30244   DOI   ScienceOn
17 Larkin, R. M., Alonso, J. M., Ecker, J. R., and Chory, J. (2003) GUN4, a regulator of chlorophyll synthesis and intracellular signaling. Science 299, 902-906   DOI   ScienceOn
18 Ahn, J.-W., Kim, M., Lim, J. H., Kim, G.-T., and Pai, H.-S. (2004) Phytocalpain controls the proliferation and differentiation fates of cells in plant organ development. Plant J. 38, 969-981   DOI   ScienceOn
19 Lopez-Juez, E., Jarvis, R. P., Takeuchi, A., Page, A. M., and Chory, J. (1998) New Arabidopsis cue mutants suggest a close connection between plastid- and phytochrome regulation of nuclear gene expression. Plant Physiol. 118, 803-815   DOI
20 Waterhouse, P. M., Wang, M.-B., and Lough, T. (2001) Gene silencing as an adaptive defence against viruses. Nature 411, 834-842   DOI   ScienceOn
21 Vance, V. and Vaucheret, H. (2001) RNA silencing in plantsdefense and counterdefense. Science 292, 2277-2280   DOI   ScienceOn
22 Strand, A., Asami, T., Alonso, J., Ecker, J. R., and Chory, J. (2003) Chloroplast to nucleus communication triggered by accumulation of Mg-protoporphyrin IX. Nature 421, 79-82   DOI   ScienceOn
23 Lee, Y. J., Kim, D. H., Kim, Y.-W., and Hwang, I. (2001) Identification of a signal that distinguishes between the chloroplast outer envelope membrane and the endomembrane system in vivo. Plant Cell 13, 2175-2190   DOI
24 Pfannschmidt, T. (2003) Chloroplast redox signals: how photosynthesis controls its own genes. Trends Plant Sci. 8, 33-41   DOI   ScienceOn
25 Xu, X. M. and Moller, S. G. (2004) AtNAP7 is a plastidic SufC like ATP-binding cassette/ATPase essential for Arabidopsis embryogenesis. Proc. Natl. Acad. Sci. USA 101, 9143-9148
26 Emanuelsson, O. and von Heijne, G. (2001) Prediction of organellar targeting signals. Biochim. Biophys. Acta 1541, 114- 119   DOI   ScienceOn
27 Xu, X. M., Adams, S., Chua, N.-H., and Moller, S. G. (2005) AtNAP1 represents an atypical SufB protein in Arabidopsis plastids. J. Biol. Chem. 280, 6648-6654   DOI   ScienceOn
28 Zhang, H., Huang, H. M., Carson, R. C., Mahmood, J., Thomas, H. M., et al. (2001) Assessment of membrane potentials of mitochondrial populations in living cells. Anal. Biochem. 298, 170-180   DOI   ScienceOn
29 Azevedo, C., Sadanandom, A., Kitagawa, K., Freialdenhoven, A., Shirasu, K., et al. (2002) The RAR1 interactor SGT1, an essential component of R gene-triggered disease resistance. Science 295, 2073-2076   DOI   ScienceOn
30 Kim, M., Ahn, J.-W., Jin, U.-H., Choi, D., Paek, K.-H., et al. (2003) Activation of the programmed cell death pathway by inhibition of proteasome function in plants. J. Biol. Chem. 278, 19406-19415   DOI   ScienceOn