• Journal of Applied Biological Chemistry
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• v.64 no.3
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• pp.213-216
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• 2021

A gene encoding thermostable cellulase A (TmCelA) was isolated from Thermotoga maritima. The open reading frame of TmCelA gene was 774 bp long which predicted to encode 257 amino acid residues with a molecular weight of 29,732 Da. To examine the biochemical properties, the TmCelA was overexpressed in E. coli BL21, and expressed protein was purified. The optimum temperature of recombinant TmCelA was 90-95 ℃, and the optimum pH of recombinant TmCelA was approximately pH 5.0. Recombinant TmCelA was stable at temperature below 90 ℃.

• Journal of Broadcast Engineering
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• v.20 no.1
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• pp.16-25
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• 2015

Even if the demands for ultra-high-quality multimedia contents are increasing, it is difficult to produce, encode, play and transport ultra-high-quality contents under the existing broadcasting environment. By the reason, various technologies for the UHD contents have been developed in order to satisfy the user's needs. In this paper, we propose a design methodology of a broadcasting system, which consists of two parts, for UHD services with two parts. At the transmit part of the proposed system can encode a video into several layered-bitstreams hierarchically, and then transport each bitstream over heterogeneous networks. The receiver part can play the received video by composing the separated bitstreams. The proposed system can adaptively provide both HD and UHD contents according to user's reception conditions by using the heterogeneous networks.

• The Journal of the Korea institute of electronic communication sciences
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• v.10 no.9
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• pp.1019-1026
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• 2015

This paper studied the source coding rule of the characters to minimize the HDB-3 scrambling for UTF-8 code. An existing source coding rule of the characters to minimize the HDB-3 scrambling in the line coder is for the source codes which are directly entered into the line coder without any transformation. Therefore the existing source coding rule can't apply the UTF-8 code which is directly came into an input of line coder. The reason is that the scrambling code in the source codes are not same as UTF-8 codes. So, if they want to analysis the scrambling occurrence situation in UTF-8 codes and make an unscrambling UTF-8 code, they should make a UTF-8 code table for the source codes, find out the scrambling occurrence codes and then encode the unscrambling source code. The source coding rule for UTF-8 code showing this paper can omit such a complicated procedure to encode an unscrambling source code.

• The KIPS Transactions:PartB
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• v.9B no.6
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• pp.715-726
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• 2002

In this paper, we construct real-time moving picture encoder based on fractal theory by using general purpose digital signal processors. The constructed encoder is implemented using two fixed-point general DSPs (ADSP2181) and performs image encoding by three stage pipeline structure. In the first pipeline stage, the image grabber acquires image data from NTSC standard image signals and stores digital image into frame memory. In the second stage, the main controller encode image dada using fractal algorithm. The last stage, output controller perform Huffman coding and result the coded data via RS422 port. The performance tests of the constructed encoder shows over 10 frames/sec encoding speed for QCIF data when all the frames are encoded. When we encode the images using the interframe and redundency based on the proposed algorithms, encoding speed increased over 30 frames/sec in average.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1655-1660
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• 2007

A metagenome is a unique resource to search for novel microbial enzymes from the unculturable microorganisms in soil. A forest soil metagenomic library using a fosmid and soil microbial DNA from Gwangneung forest, Korea, was constructed in Escherichia coli and screened to select lipolytic genes. A total of seven unique lipolytic clones were selected by screening of the 31,000-member forest soil metagenome library based on tributyrin hydrolysis. The ORFs for lipolytic activity were subcloned in a high copy number plasmid by screening the secondary shortgun libraries from the seven clones. Since the lipolytic enzymes were well secreted in E. coli into the culture broth, the lipolytic activity of the subclones was confirmed by the hydrolysis of p-nitrophenyl butyrate using culture supernatant. Deduced amino acid sequence analysis of the identified ORFs for lipolytic activity revealed that 4 genes encode hormone-sensitive lipase (HSL) in lipase family IV. Phylogenetic analysis indicated that 4 proteins were clustered with HSL in the database and other metagenomic HSLs. The other 2 genes and 1 gene encode non-heme peroxidase-like enzymes of lipase family V and a GDSL family esterase/lipase in family II, respectively. The gene for the GDSL enzyme is the first description of the enzyme from metagenomic screening.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.6
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• pp.2489-2493
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• 2015

Background: Loss of heterozygosity (LOH) on chromosomal regions is crucial in tumor progression and this study aimed to identify genome-wide LOH in pancreatic cancer. Materials and Methods: Single-nucleotide polymorphism (SNP) profiling data GSE32682 of human pancreatic samples snap-frozen during surgery were downloaded from Gene Expression Omnibus database. Genotype console software was used to perform data processing. Candidate genes with LOH were screened based on the genotype calls, SNP loci of LOH and dbSNP database. Gene annotation was performed to identify the functions of candidate genes using NCBI (the National Center for Biotechnology Information) database, followed by Gene Ontology, INTERPRO, PFAM and SMART annotation and UCSC Genome Browser track to the unannotated genes using DAVID (the Database for Annotation, Visualization and Integration Discovery). Results: The candidate genes with LOH identified in this study were MCU, MICU1 and OIT3 on chromosome 10. MCU was found to encode a calcium transporter and MICU1 could encode an essential regulator of mitochondrial $Ca^{2+}$ uptake. OIT3 possibly correlated with calcium binding revealed by the annotation analyses and was regulated by a large number of transcription factors including STAT, SOX9, CREB, NF-kB, PPARG and p53. Conclusions: Global genomic analysis of SNPs identified MICU1, MCU and OIT3 with LOH on chromosome 10, implying involvement of these genes in progression of pancreatic cancer.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.16 no.2
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• pp.161-170
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• 2017

The balise is a device for the railroad signal control systems, which is installed between both rail. The balise sends fixed or variable data, named telegram, to the train with wireless method. The telegram includes the position information, the movable distance under the signal status, the gradient, the speed, the temporary speed limit, etc. This research is on a design of the DBPL encoder for the balise. Normally the DBPL encoder for the balise is with the ASIC or FPGA technology. In this research, the DBPL encoder is designed with commercial low power operable micro-controller. The firmware(logic level encode) and the SPI Bus function block(physical level output) of the micro-controller are used for the DBPL encode. Under the european standard, the required working speed of the DBPL encoder is 564.48Kbps. The DBPL encoder of this research is tested under the speed of 564.48Kbps, and it worked properly.

• The Transactions of the Korea Information Processing Society
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• v.3 no.5
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• pp.1307-1316
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• 1996

In this paper, we propose a vector quantization method which uses a dynamic address mapping based on exploring the high interblock correlation. In the proposed method, we reduce bit-rate by defining an address transform function, which maps a VQ address of an input block which will be encoded into a new address in the reordered codebook by using side match error. In one case that an original address can be transformed into a new transformed address which is lower than the threshold value, we encode the new address of the transformed convector, and in the other case we encode the address of the original convector which is not transformed. Experimental results indicate that the proposed scheme reduces the bit-rate by 45~50% compared with the ordi-nary VQ method forimage compression, at the same quality of the reconstructed image as that of the ordinary VQ system.

• BMB Reports
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• v.33 no.3
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• pp.195-201
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• 2000

A human gene has been reported that may encode the enzyme acetohydroxyacid synthase. Previously this enzyme was thought to be absent from animals although it is present in plants and many microorganisms. In plants, this enzyme is the target of a number of commercial herbicides and the use of these compounds may need to be reassessed if the human enzyme exists and proves to be susceptible to inhibition. Here we report the construction of several plasmid vectors containing the cDNA sequence for this protein, and their expression in Escherichia coli. High levels of expression were observed, but most of the protein proved to be insoluble. The small amounts of soluble protein contained little or no acetohydroxyacid synthase activity. Attempts to refold the insoluble protein were successful insofar as the protein became soluble. However, the refolded protein did not gain any acetohydroxyacid synthase activity. In vivo complementation tests of an E. coli mutant produced no evidence that the protein is active. Incorrect folding, or the lack of another subunit, may explain the data but we favor the interpretation that this gene does not encode an acetohydroxyacid synthase.

• Journal of the Korean Institute of Telematics and Electronics
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• v.27 no.3
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• pp.13-20
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• 1990

This paper describes an interframe image coding using motion compensated and classified vector quantizer (MC-CVQ). It is essential to carefully encode blocks with significant pels in motion compensated vector quantizers (MCVQ). In this respect, we propose a new CVQ algorithm which is appropriate to the coding of interframe prediction error after motion compensation. In order to encode an image efficiently at a low bit rate, we partition each block, which is the processing element in MC, into equally sized 4 vectors, and classify vectors into 15 classes according to the position of significant pels. Vectors in each class are then encoded by the vector quantizer with the codebook independently designed for the class. The computer simulation shows that the signal-to-noise ratio and the average bit rate of MC-CVQ are 35-37dB and 0.2-0.25bit/pel, respectively, for the videophone or video conference type image.