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http://dx.doi.org/10.3839/jabc.2021.030

Characterization of the recombinant cellulase A from Thermotoga maritima

Kim, Chung Ho (Department of Food and Nutrition, Seowon University)

Publication Information

Journal of Applied Biological Chemistry / v.64, no.3, 2021 , pp. 213-216 More about this Journal

Abstract

A gene encoding thermostable cellulase A (TmCelA) was isolated from Thermotoga maritima. The open reading frame of TmCelA gene was 774 bp long which predicted to encode 257 amino acid residues with a molecular weight of 29,732 Da. To examine the biochemical properties, the TmCelA was overexpressed in E. coli BL21, and expressed protein was purified. The optimum temperature of recombinant TmCelA was 90-95 ℃, and the optimum pH of recombinant TmCelA was approximately pH 5.0. Recombinant TmCelA was stable at temperature below 90 ℃.

Keywords

Cellulase; Cellulose; Thermostable; Thermotoga maritima;

Citations & Related Records

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