• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2008년도 International Meeting of the Microbiological Society of Korea
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• pp.86-88
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• 2008

• Mass Spectrometry Letters
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• 제9권3호
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• pp.77-80
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• 2018

The focus of this paper is to present techniques to overcome certain difficulties in quantitative analysis with a time-of-flight mass spectrometer (TOF-MS). The method is based on conventional solid-phase extraction, followed by reversed-phase ultra high performance liquid chromatography of the extract, and mass spectrometric analysis. The target compounds included atenolol, atrazine, caffeine, carbamazepine, diclofenac, estrone, ibuprofen, naproxen, simazine, sucralose, sulfamethoxazole, and triclosan. The matrix effects caused by high concentrations of organic compounds in wastewater are especially significant in electrospray ionization mass spectroscopy. Internal-standard calibration with isotopically labeled standards corrects the results for many matrix effects, but some peculiarities were observed. The problems encountered in quantitation of carbamazepine and triclosan, due to nonlinear calibration were solved by changing the internal standard and using a narrower mass window. With simazine, the use of a quadratic calibration curve was the best solution.

• 한국지하수토양환경학회지:지하수토양환경
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• 제24권2호
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• pp.8-22
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• 2019

Among numerous chemicals used globally, the number of emerging contaminants is increasing. Numerical modeling for contaminant fate and transport in the subsurface is critical to evaluate environmental and health risk. In general, such models require physicochemical properties of contaminants as input values, which can be found in numerous chemical databases (DB). However, there exist lack of information specific to recently emerging contaminants, which requires estimation of physicochemical properties using regression programs. The purpose of the study is to introduce the workflow for identifying physicochemical properties of potential contaminants utilizing numerous chemical DBs, which frequently lists up potential contaminants for estimating chemical behavior. In this review paper, details of several chemical DBs such as KISChem, TOXNET, etc. and regression programs including EPI $Suite^{TM}$, ChemAxon, etc. were summarized and also benefit of using such DBs were explained. Finally, a few examples were introduced to estimate predominant phase, removal ratio, partitioning, and eco-toxicities by searching or regressing physicochemical properties.

• 한국물환경학회지
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• 제28권6호
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• pp.902-910
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• 2012

The photodegradation characteristics of 30 pharmaceuticals were investigated by batch experiments using Ultraviolet (UV) reactor. The investigated pharmaceuticals include antibiotics, analgesics and antiarrhythmic agents etc. Tested water was prepared by simultaneously spiking 30 pharmaceuticals into pure water, and each experiment was conducted using 3 types of UV lamps. As a result, batch experiments showed that reactions of all the investigated pharmaceuticals followed pseudo-first order reaction regardless of the applied UV lamps. Among the pharmaceuticals, Cyclophosphamide, 2-Quinoxaline carboxylic acid and Clarithromycin proved to be the most UV-resistant compounds. Contrarily, Ceftiofur, Diclofenac and Ketoprofen were easily degraded by all the UV lamps. Dissolved organic carbon (DOC) concentration hardly changed although the concentration of the pharmaceuticals concentration gradually decreased with time, indicating that the degradation of parent pharmaceuticals may produce their intermediates during UV treatment.

• 상하수도학회지
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• 제27권6호
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• pp.797-803
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• 2013

Recently, a number of countries are now considering the reuse of effluents from wastewater treatment for various water applications. To improve the reuse of wastewater effluent, the development of appropriate micro-pollutant removal technology is necessary. Although several researche have been studied for removing micro-pollutants in water, little study has been conducted for the removal of emerging contaminant such as antibiotic resistant genes (ARGs) by disinfection processes. Therefore, the aim of this study is to compare the capacity of disinfection technologies such as chlorination, ozone, and electron beam, for removing antibiotic resistant bacteria (ARB) and ARGs. Based on this study, better ARG removal can be achieved by ozonation and electron beam. Relatively, high CT values of chlorination or ozonation are needed to remove ARB and ARG compared to conventional pathogens.

• 자원환경지질
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• 제54권3호
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• pp.353-364
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• 2021

국내 지질학의 연구 분야는 20세기 중반 이후부터 꾸준하게 발전되어왔다. 학술지 "자원환경지질"은 국내 지질학을 대표하는 역사가 긴 학술지로 지질학을 바탕으로 하는 융복합연구 논문이 게재되고 있다. 본 연구는 학술지 "자원환경지질"에 게재된 논문을 대상으로 문헌 고찰(literature review)을 수행하여 지질학의 역사와 발전에 대해 논의하고자 한다. 1968년부터 2020년까지 총 2,571편의 논문 제목, 주제어, 다국어 초록을 수집하였으며, Latent Dirichlet Allocation (LDA) 기반 토픽모델링을 실시하여 연구 주제를 분류하고 연구 동향과 주제간 연관성을 확인하였다. 학술지 "자원환경지질"은 총 8개의 연구주제('암석학 및 지구화학', '수문학 및 수리지질학', '광상학', '화산학', '토양오염 및 복원학', '기초지질 및 구조지질학', '지구물리 및 물리탐사', '점토광물')로 분류할 수 있었다. 1994년 이전에는 '광상학', '화산학', '기초지질 및 구조지질학'의 연구주제들이 활발하게 연구되었으며, 이후 '수문학 및 수리지질학', '토양오염 및 복원학', '지구물리 및 물리탐사', '점토광물'의 연구주제들이 성행하였다. 연관성분석(network analysis)결과, 학술지 "자원환경지질"은 '광상학'을 기반으로 융복합적 연구 논문들이 게재되었다는 것을 확인하였다. 본 연구의 결과는 지질학을 다루는 연구자들에게 문헌 고찰의 새로운 방법론을 제시하여 지질학의 역사에 대한 이해를 제공했음에 의의가 있다.

• BMB Reports
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• 제53권11호
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• pp.565-575
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• 2020

Gene therapy is emerging as a treatment option for inherited genetic diseases. The success of this treatment approach greatly depends upon gene delivery vectors. Researchers have attempted to harness the potential of viral vectors for gene therapy applications over many decades. Among the viral vectors available, gutless adenovirus (GLAd) has been recognized as one of the most promising vectors for in vivo gene delivery. GLAd is constructed by deleting all the viral genes from an adenovirus. Owing to this structural feature, the production of GLAd requires a helper that supplies viral proteins in trans. Conventionally, the helper is an adenovirus. Although the helper adenovirus efficiently provides helper functions, it remains as an unavoidable contaminant and also generates replication-competent adenovirus (RCA) during the production of GLAd. These two undesirable contaminants have raised safety concerns and hindered the clinical applications of GLAd. Recently, we developed helper virus-free gutless adenovirus (HF-GLAd), a new version of GLAd, which is produced by a helper plasmid instead of a helper adenovirus. Utilization of this helper plasmid eliminated the helper adenovirus and RCA contamination in the production of GLAd. HF-GLAd, devoid of helper adenovirus and RCA contaminants, will facilitate its clinical applications. In this review, we discuss the characteristics of adenoviruses, the evolution and production of adenoviral vectors, and the unique features of HF-GLAd as a new platform for gene therapy. Furthermore, we highlight the potential applications of HF-GLAd as a gene delivery vector for the treatment of various inherited genetic diseases.

• 한국환경보건학회지
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• 제37권4호
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• pp.289-297
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• 2011

Objectives: To estimate the microbial contaminant load discharged from livestock farms, we randomly selected livestock farmers of cattle, swine, and fowl and collected bacterial strains from domestic animals' feces and compost samples. Recently, as multi-antibiotic-resistant bacteria and super bacteria showing resistance to a variety of antibiotics have been reported one after another, the ecological and health hazard of antibiotic-resistant bacteria is emerging as an important issue. Methods: Monitored indicator microorganism constituents were totak coliform (TC), fecal coliform (FC), and aerobic bacteria. The multi-antibiotic-resistant bacteria were identified from investigated indicator microorganisms by 16S rRNA sequencing. Results: By microbiological analysis, the largest population of aerobic bacteria ($1.5{\times}10^5$ CFU/g) was found in cattle fecal compost, and total coliforms ($1.1{\times}10^7$ CFU/g) and fecal coliforms ($1.0{\times}10^5$ CFU/g) were found primarily in swine fecal compost, while the lowest population was found in fowl fecal compost. Among the 67 strains separated from aerobic bacteria, five strains expressing high antibiotic resistance were selected in each sample. We found the multi-antibiotic resistant strains to be Shigella boydii, Staphylococcus lentus, Acinetobacter sp. and Brevibacterium luteolum. Conclusions: These results suggest that increasing numbers of multi-antibiotic-resistant bacteria in the environment have a close relation to the reckless use of antibiotics with livestock.

• 한국지구물리탐사학회:학술대회논문집
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• 한국지구물리탐사학회 2001년도 정기총회 및 제3회 특별지포지움
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• pp.23-33
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• 2001

국내 토양과 지하수 오염이 심각한 상황에 도달하였다는 것은 널리 알려진 사실이다. 선진국에서는 20 여년 전부터 그 심각성을 이해하고 환경복원 사업이라는 새로운 분야를 발전시켜 왔으나 많은 큰 문제들의 해결로 현재는 사업의 소강상태에 있다고 본다. 토양과 지하수는 우리가 깨끗이 보존하여야 할 자연 자원이다. 토양과 지하수 환경사업은 오염 예방, 조사, 복원 사업으로 나눌 수 있는데 본 발표에서는 조사와 복원에 관한 문제들을 고찰하고자 한다. 국내에서의 복원 사업은 시작인 단계에 있으며 국가 정책과 투자의지에 따라 그 전망은 결정될 것이다. 복원은 평가로부터 시작 오염 물질의 위해도와 경제성 등을 고려한 복원방법의 선정과 실행으로 끝나게 된다. 물론 물리탐사도 조사 단계에서 사용되는 도구이지만 그 필요성은 경제적일 뿐만 아니라 다른 방법에서는 얻을 수 없는 결과를 보여줄 수 있기 때문이다. 물리탐사와 마찬가지로 전 조사-복원과정에서 토양-지질-지하수의 이해가 정확한 해석과 판단을 위하여 선행되어야 한다. 강조되어야 할 것은, 조사-복원 사업은 연구와 개발(R&D)과는 달리 복원 목표를 달성할 수 있는 여러 방법 중 가장 경제적 (cost-effectiveness)인 방법을 선택하여 사업을 수행함으로써 고객을 만족시켜야 한다는 것이다.

• Environmental Analysis Health and Toxicology
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• 제26권
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• pp.12.1-12.7
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• 2011

Objectives: Perchlorate is an emerging contaminant that is found everywhere, including various foods. Perchlorate is known to disturb the production of thyroid hormones and leads to mental disorders in fetuses and infants, as well as metabolic problems in adults. In this study, we attempted to establish an LC-MS/MS method for measuring perchlorate in dairy products and used this developed method to investigate perchlorate levels in Korean milk and yogurt samples. Methods: The developed method of perchlorate analysis requires a shaker and 1% acetic acid/acetonitrile as the extracting solvent. Briefly, the samples were extracted and then centrifuged (4000 rpm, 1hour), and the supernatant was then passed through a $Envi^{TM}$ Carb SPE cartridge that had been prewashed sequentially with 6 mL of acetonitrile and 6 mL of 1% acetic acid in water. The final volume of the sample extract was adjusted to 40 mL with reagent water and the final sample was filtered through a 0.20-${\mu}m$ pore size PTFE (Polytetrafluoroethylene) syringe filter prior to LC-MS/MS. Results: The average levels of perchlorate in milk and yogurt samples were $5.63{\pm}3.49\;{\mu}g/L$ and $3.65{\pm}2.42\;{\mu}g/L$, respectively. The perchlorate levels observed in milk samples in this study were similar to those reported from China, Japan, and the United States. Conclusions: The exposure of Koreans to perchlorate through the consumption of dairy products was calculated based on the results of this study. For all age groups, the calculated exposure to perchlorate was below the reference of dose (0.7 ${\mu}g/kg$-day) proposed by the National Academy of Science, USA, but the perchlorate exposure of children was higher than that of adults. Therefore, further investigation of perchlorate in other food samples is needed to enable a more exact assessment of exposure of children to perchlorate.