• 한국약용작물학회지
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• 제20권4호
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• pp.222-230
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• 2012

We evaluated the inhibitory effects of extracts and components of Geranium thunbergii on aldose reductase (AR) and galactitol formation in rat lenses with high levels of galactose as a part of our ongoing search of natural sources for therapeutic and preventive agents for diabetic complications. The inhibitory effects of water, methanol and ethanol extracts of G. thunbergii on rat lens AR (RLAR) were determined. Comparing inhibitory effects of various solvent extracts, ethanol extract showed RLAR inhibitory activity ($IC_{50}$ values, 5.24 and $6.39{\mu}g/m{\ell}$, respectively). The ethanol extract was fractionated to chloroform, ethyl acetate and water. Of these, the ethyl acetate fraction from ethanol extract of G. thunbergii exhibited RLAR inhibitory activity ($IC_{50}$ value, $2.64{\mu}g/m{\ell}$). In order to identify the bioactive components of ethyl acetate soluble fraction of ethanol extract from G. thunbergii, eight compounds, namely gallic acid (1), protocatechuic acid (2), p-hydroxybenzoic acid (3), brevifolin carboxylic acid (4), geraniin (5), ellagic acid (6), kaempferol-3-O-arabinofuranosyl-7-O-rhamnopyranoside (7), kaempferitrin (8) were isolated. The isolates were subjected to in vitro bioassays to evaluate their inhibitory activity on RLAR and galactitol formation in rat lenses. The ellagic tannins (5, 6) and flavonoid (7) exhibited strong inhibitory effects on RLAR. Also, these three compounds (5, 6 and 7) suppressed galactitol accumulation in rat lens under high galactose conditions, demonstrating the potential to prevent galactitol accumulation exo vivo. These results suggest that the extracts and components of G. thunbergii are a promising agent in the prevention or treatment of diabetic complications.

• 한국식품저장유통학회지
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• 제20권3호
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• pp.342-347
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• 2013

본 연구는 고흥산과 수입산 석류의 화학적 성분분석 및 생리활성 기능을 분석하여 기능성 물질의 소재 개발과 착즙 후 버려지는 부산물 활용 방안에 기여하고자 하였다. 석류 과즙 중 주요 유기산은 citric acid, malic acid가 확인되었으며 주요 유리당으로는 fructose, glucose가 확인되었다. 석류의 폴리페놀 함량은 고흥산 과피에서 149.91 mg/g으로 가장 높았고 가장 낮은 함량을 보인 과즙보다는 약 100배 정도 높게 함유되어 있었다. 석류의 부위별 ellagic acid 함량을 분석한 결과 고흥산 과피에서 14.22 mg/g으로 미국산에 비해 두 배 이상 높게 나타났으며, 고흥산과 미국산 모두 과즙이나 씨 보다는 과피에서 ellagic acid 함량이 월등히 높게 나타났다. 석류 부위별 추출물은 농도 의존적으로 항산화 활성이 증가하였으며, 고흥산과 미국산 모두 과피, 씨, 과즙 순으로 높은 활성도를 나타냈다. 고흥산과 미국산 부위별 추출물의 전립선암 세포증식 억제능을 분석한 결과 산지에 관계없이 과피, 과즙, 씨 순으로 암세포 성장억제능이 높게 나타났으며 특히 과즙과 씨에서는 고흥산이 미국산에 비해서 암세포 성장억제능이 더 우수하였으며 과피 추출물에서는 미국산이 더 우수하였다.

• 한국식품과학회지
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• 제51권6호
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• pp.517-523
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• 2019

본 연구에서는 다양한 품종의 딸기 추출물로부터 천연물을 이용한 천연 항산화제 개발을 위하여 농업기술원에서 시료를 제공받아 항산화 활성을 평가하고, ellagic acid 함량 분석 및 분석법 검증을 위한 밸리데이션을 수행하였다. 총 폴리페놀 함량은 22.77-107.61 mg/100 g FW로 다양하게 나타났으며, 플라보노이드 함량 또한 17.58-44.12 mg/100 g FW으로 다양하게 나타났다. 총 폴리페놀 함량 및 플라보노이드 함량 결과 Aiberry와 Elie star에서 가장 많은 총 폴리페놀과 플라보노이드 함량을 나타내었다. DPPH free radical scavenging activity은 1540.6-1124.0 μmol TEAC/100 g FW 범위를 나타내었고, Derunoka가 1.541 μmol TE/100 g FW로 가장 높은 값을 나타내었다. ABTS free radical scavenging activity 실험결과 6352.3-4592.3 μmol TEAC/100 g FW의 범위를 나타내었으며, FA23 추출물이 가장 높은 활성을 보였다. Ellagic acid 표준물질에 대한 검량선의 상관계수(R²)값은 0.9998-1.0000으로 매우 높은 직선성을 보였으며, 딸기 추출물의 ellagic acid 함량은 9.2-22.9 μg/mL을 나타냈으며, 그 중 Asca 추출물이 22.9 μg/mL로 가장 높은 ellagic acid 함량을 나타내었다. 밸리데이션 결과 정확성은 평균 100-101%의 회수율을 나타내었고, 정밀성 또한 농도별로 10 μg/mL에서는 5.31, 5.30%, 50 μg/mL에서는 0.66, 0.88%, 100 μg/mL에서는 0.20, 0.82%의 RSD값을 나타내었다. 본 연구의 결과를 종합하여 보면 다양한 딸기품종 추출물은 높은 항산화 효능을 나타내며, 이는 자유라디칼의 산화를 억제하는 총 폴리페놀 및 플라보노이드의 높은 함량에 기인하는 것으로 사료된다. 또한 천연항산화제 표준화를 위한 지표성분으로서 ellagic acid 정량분석 및 밸리데이션을 수행함으로써 본 실험에서 사용된 딸기추출물의 천연 항산화제로서의 개발 가능성과 함량분석에 대한 기준설정을 위한 기초자료를 시사한다.

• Mass Spectrometry Letters
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• 제11권4호
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• pp.82-89
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• 2020

A systematic isolation and characterization study for Cassia auriculata (CA) seeds resulted in identifying antidiabetic compounds 1,3,8-trihydroxyanthraquinone and quercetin, quercetin-3-O-rutinoside, gallic acid, caffeic acid, ferulic acid, and ellagic acid. The ultra-high-performance liquid chromatography based triple quadrupole mass spectrometry methodology was developed and validated for simultaneous identification and confirmation of these compounds from CA seeds. Multiple reaction monitoring (MRM) based quantification method was developed with MRM optimizer software for MS1 and MS2 mass analysis. The method was optimized on precursor ions and product ions with the ion ratio of each compound. The calibration curves of seven bioactive analytes showed excellent linearity (r2 ≥ 0.99). The quantitation results found precise (RSD, < 10 %) with good recoveries (84.58 to 101.42%). The matrix effect and extraction recoveries were found within the range (91.66 to 102.11%) for the CA seeds. This is the first MS/MS-based methodology applied to quantifying seven antidiabetic compounds in CA seeds and its extract for quality control purposes.

• 한국미생물·생명공학회지
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• 제47권1호
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• pp.54-63
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• 2019

Rubus coreanus Miquel (RCM), also known as Korean blackberry or bokbunja, is used as a South Korean traditional medicine to treat acne and inflammatory skin conditions. The antimicrobial activity of RCM root and its active compounds remain unclear. In this study, we prepared a 50% ethanol fraction, ethyl acetate fraction, and acid-treated ethyl acetate fraction (aglycone fraction) of RCM root, and evaluated antibacterial activities against the skin pathogens Staphylococcus aureus, Pseudomonas acnes, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. In a paper disc assay, all fractions of RCM root showed antimicrobial activities against the five skin pathogens. The ethyl acetate fraction displayed 6-, 12-, and 2-fold higher minimal inhibitory concentration (MIC) than the 50% ethanol fraction against S. aureus, E. coli, and P. acnes, respectively. The aglycone fraction displayed 2-fold higher MIC than methyl paraben against P. acnes, S. aureus, E. coli, and P. aeruginosa. The ethyl acetate fraction displayed a minimal bactericidal concentration (MBC) similar to that of methyl paraben, and the aglycone fraction showed 2- to 4-fold higher MBCs than those of methyl paraben. In particular, the ethyl acetate fraction was not cytotoxic and showed thermal stability after incubation at high temperatures ($60-121^{\circ}C$). Finally, the ethyl acetate fraction was separated and four components were identified: procyanidin C, propelagonidin dimer, ellagic acid, and methyl ellagic acid acetyl pentose. The compounds showed high antibacterial activities. These results suggest that RCM root is potentially applicable as a natural preservative in cosmetics.

• 생약학회지
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• 제27권2호
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• pp.142-145
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• 1996

We have previously reported the isolation of seven hydrolyzable tannins and nine flavonoids from Euphorbia pekinensis Ruprecht. Further investigation about the same plant has led to the isolation of kaempferol, quercetin, kaempferol $3-O-(2'-O-galloyl-{\beta}-_D-glucoside)$, quercetin 3-O-(2'-O-galloylrutinoside), ellagic acid and acetonyl geraniin. These compounds were isolated from this plant for the first time.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2001년도 International Symposium on Dietary and Medicinal Antimutgens and Anticarcinogens
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• pp.110-110
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• 2001

Inner shell of chestnut (Castanea Mollissima, Fagaceae) is well-known anti-wrinkle agent, which has been used for long time in the treatment of skin aging. In this study, the extract of chestnut inner shell (CMIE) and its major component, ellagic acid (EA), were studied for their protective effects against free radical generation and hydrogen peroxide-induced oxidative DNA damages in the mammalian cells.(omitted)

• Rapid Communication in Photoscience
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• 제2권1호
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• pp.28-30
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• 2013

The extract of Punica granatum peel was separated and confirmed by HPLC, and punicalagin, one of the extract, was better antibacterial activity against Salmonella.

• 약학회지
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• 제33권6호
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• pp.377-379
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• 1989

The methylated compound of ellagic acid was isolated from Potentilla chinensis (Rosaceae). The isolated compound was 3, 3', 4-tri-O-methylellagic acid, [2, 3, 7-trimethoxy-8-hydroxy[1] benzopyrano-[5, 4, 3, cde][1] benzopyran-5, 10-dione], $C_{17}H_{12}O_8$, m.p. $293-295^{\circ}C$. The isolation of trimethylellagic acid was conducted by the column chromatography and the identification of the compound was carried out by the methods of IR, NMR and MS spectroscopy.

• 원예과학기술지
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• 제29권3호
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• pp.172-180
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• 2011

The net photosynthetic rate of 'Seolhyang' strawberry plants was measured daily for 7 days after treatment at three UV illumination dosages (0, 9.8, and 29.5 $kJ{\cdot}m^{-2}$). The net photosynthetic rates of the strawberry plants with 9.8 and 29.5 $kJ{\cdot}m^{-2}$ UV light illumination decreased by 20.2 and 61.4%, respectively, at 7 days after UV light treatments. UV treatments with two illumination dosages (7.9 and 15.7 $kJ{\cdot}m^{-2}$) altered the phenolic compounds contents during the cultivation period when compared to those in the control fruits. The anthocyanin content with 7.9 $kJ{\cdot}m^{-2}$ UV light illumination of 'Seolhyang' increased by 18.7% compared with those in control fruits at the 11 DAT. However, the anthocyanin content of 'Maehyang' was not significantly different among treatments during experiment period. The highest level of ellagic acid was found with 7.9 $kJ{\cdot}m^{-2}$ UV light illumination in both cultivars at the 11 DAT. Our results showed that strawberries illuminated with UV light during cultivation period had higher bioactive compounds contents than control fruits. These results suggest that UV light treatments may be a useful non-chemical way of promoting strawberry fruits quality.