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http://dx.doi.org/10.9721/KJFST.2019.51.6.517

Antioxidant activities and Validation of Analytical Method of Marker compounds in strawberry fruits from various cultivars  

Yong, Ye Seul (Department of Pharmaceutical Engineering, Dongshin University)
Lee, Songmi (Department of food and Nutrition, Dongshin University)
Byun, Na-Young (Department of Pharmaceutical Engineering, Dongshin University)
Sun, Sangouk (Department of Pharmaceutical Engineering, Dongshin University)
Kim, Min-Jung (Jeollanamdo Agricultural Research & Extension Services)
Jang, Seo Woo (Jeollanamdo Agricultural Research & Extension Services)
Jang, Won Suk (Chungcheongnamdo Agricultural Research & Extension Services)
Lee, Sun Yi (National Institute of Horticultural and Herbal Science)
Yim, Soon-Ho (Department of Pharmaceutical Engineering, Dongshin University)
Publication Information
Korean Journal of Food Science and Technology / v.51, no.6, 2019 , pp. 517-523 More about this Journal
Abstract
In this study, we investigated antioxidant capacity of 20 strawberry extract using the DPPH and ABTS assay and HPLC-DAD validation method. The total polyphenolic and flavonoids contents of 20 strawberry extracts were 22.77-107.61 mg TAE/100 g FW and 17.58-44.12 mg QE/100 g FW. The Aiberry and Elie star showed the highest total polyphenol and flavonoid contents, respectively. The DPPH radical scavenging activity of what was 1540.6-1124.0 μmol TEAC/100 g FW and Derunoka showed the highest activity. The ABTS radical scavenging activity was 6352.3-4592.3 μmol TEAC/100 g FW and FA23 showed the highest activity. The HPLC-DAD method for the quantitation of ellagic acid results showed high linearity in various concentration ranges, and the limit of detection was 2.35 μg/mL. The limit of quantification was 7.12 μg/mL. Relative standard deviation values from intra-and inter-day precision were less than 5.31%. Recovery rate at 10, 50, and 100 μg/mL, respectively, were 100.0-101.0% with relative standard deviation (RSD) values less than 5.30%. These results provide viable information for the validation of antioxidant capacity in strawberry fruits.
Keywords
strawberry; ellagic acid; antioxidant; validation;
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