• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2002년도 국제심포지엄
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• pp.71-71
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• 2002

Promoters for milk proteins have been used far producing transgenic animals due to their temporal and spatial expression patterns. ${\beta}$-casein, a calcium-sensitive casein, is a major milk protein that corresponds ca. 30 per cent of total milk protein. Expression of ${\beta}$-casein is controlled by lactogenic hormones such as prolactin (PRL), composite response elements (CoREs) and transcription factors. CoREs are clusters of transcription factor binding sites containing both positive and negative regulatory elements. ${\beta}$-casein gene promoter contains various regions (CoREs) for gene transcription. We analyzed the promoter region by mutagenesis using exonuclease III and linker-scanning. Transcription control elements usually are positioned in 5'-flanking region of the gene. However, in some cases, these elements are located in other regions such as intron 1. The nucleotide sequences of ${\beta}$-casein promote. region has been reported (E12614). However, the properties of the promoter is not yet clear. In this study, we plan to investigate the properties of cis-regulating elements of porcine ${\beta}$-casein by mutation analysis and expression analysis using dual-luciferase repoter assay system.

• 생명과학회지
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• 제16권7호
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• pp.1188-1194
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• 2006

노년에 나타나는 정신병적 증상의 가장 흔한 원인 질환으로 만성적이고 서서히 악화되는 진행성이면, 기억력, 사고력, 학습능력 및 판단력 등의 손상을 포함한 인지기능의 장애이다. 고령화 사회가 도래하면서 노인성 치매환자가 매년 급격히 늘고 있으며 매년 수만 명이 노인성치매에 걸려 본인뿐 아니라 가족까지도 많은 고통에 시달리고 있다. 특히 노인성 치매의 경우는 환자 본인의 문제가 아니라 젊은 노동력을 환자의 보호자로 필요함으로 국가적 노동력의 손실로 이어지고 있다. 현재 연구에서 우리는 노인성 치매와 transposable elements와의 상호관계를 밝히기 위하여 공개된 유전자 데이터베이스에서 EST (expressed sequence tags)를 이용하여 생물정보학적 인 분석방법과 프로그램을 이용하여 치매의 원인으로 추정되는 후보유전자들을 찾아내었다. 이러한 분석을 통하여 치매환자에서 transposable elements의 발현으로 인해 유전자의 발현에 변화를 가지는 98개의 후보 유전자를 찾아내었다. 노인성질환인 치매와 transposable elements의 분석방법을 이용하면 치매의 원인을 규명하는데 많은 도움이 될 것이다.

• Food Science and Biotechnology
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• 제15권4호
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• pp.485-493
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• 2006

Lactococcus species are noninvasive and nonpathogenic microorganisms that are widely used in industrial food fermentation and as well-known probiotics. They have been modified by traditional methods and genetic engineering to produce useful food-grade materials. The application of genetically modified lactococci in the food industry requires their genetic elements to be safe and stable from integration with endogenous food microorganisms. In addition, selection for antibiotic-resistance genes should be avoided. Several expression and secretion signals have been developed for the production and secretion of useful proteins in lactococci. Food-grade systems composed of genetic elements from lactic acid bacteria have been developed. Recent developments in this area have focused on food-grade selection markers, stabilization, and integration strategies, as well as approaches for controlled gene expression and secretion of foreign proteins. This paper reviews the expression and secretion signals available in lactococci and the development of food-grade markers, food-grade cloning vectors, and integrative food-grade systems.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제11권2호
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• pp.63-71
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• 2005

The purpose of this study is to find the complementary idea of the psychological elements in aesthetic expression of architectural space in the sense of healing. Marks $L\ddot{u}scher$, the swiss psychologist suggested reasonable categories of emotional character according to the couple combinations among Content, Self-Esteem, Confidence, and Liberty. His theory on healing is not only the concept but the practical method to understand and control the conflict between contrary elements of emotion. Compared to the Freud's objective theory of cause and consequence, his idea is focused on the subjective and autonomous harmony. This theory based on complementary idea is applied to psychological attitude of architects on Spatial Expression in this study. Refuge, Prospect, Flow and Void are assumed as equivalent spatial elements to the four major emotional conditions suggested by $M.L\ddot{u}scher$. The couple combinations among them also characterize the architectural characters definitely and it is expected to be the reasonable criteria in analysing aesthetic value and motive of creating human space.

• 식물병연구
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• 제26권1호
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• pp.38-43
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• 2020

The genome of the rice blast fungus Magnaporthe oryzae contains several types of transposable elements (TEs), and some TEs cause genetic variation that allows M. oryzae to evade host detection. We studied how five abundant TEs in rice pathogens, Pot3, Pot2, MAGGY, Line-like element (MGL) and Mg-SINE, are expressed under diverse conditions related to growth, development, and stress. Expression of Pot3 and Pot2 was activated in germinated conidia and mycelia treated with tricyclazole. Retrotransposon MAGGY was highly expressed in appressoria and tricyclazole-treated mycelia. MAGGY and Pot2 were also activated during the early and late stages of perithecia development. MGL was up-regulated in conidia and during conidial germination but not during appressorium formation. No noticeable expression of Mg-SINE was observed under most conditions. Our results should help investigate if and how condition-specific expressions of some TEs contribute to the biology and evolution of M. oryzae.

• 디지털융복합연구
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• 제12권12호
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• pp.243-248
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• 2014

본 논문에서는 사람 얼굴의 표정을 인식하기 위하여 판단 요소를 선정하고 판단 요소의 변화 상태를 파악하여 표정을 인식하는 방법을 제안한다. 판단 요소를 선정하기 위하여 이미지 영역 분할 방법을 사용하며, 판단 요소의 변화율을 이용하여 표정을 판단한다. 표정을 판단하기 위하여 90명의 표정을 데이터베이스화하여 비교하였고, 4개의 표정(웃음, 화남, 짜증, 슬픔)을 인식하는 방법을 제안한다. 제안한 방법은 시뮬레이션을 실시하여 판단 요소 검출 성공률과 표정 인식률을 통해 검증한다.

• 융합정보논문지
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• 제10권11호
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• pp.265-271
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• 2020

본 연구는 네일아트의 시각적 표현을 위해 공간을 구성하는 조형의 기본요소인 점, 선, 면을 적용한 네일아트를 제시하여 네일아트 디자인의 창의적인 발상과 예술적인 영역의 확대에 도움이 되고자 한다. 연구방법은 전문서적과 선행연구, 인터넷 웹사이트를 통해 조형요소의 개념과 특성을 고찰하였다. 다양한 표현 기법과 함께 점, 선, 면의 특성이 네일아트에 어떻게 적용되며 시각메세지로 표현되고 있는지를 각 특성에 따라 분석하여 총 6작품을 제작하였다. 본 연구는 조형요소인 점, 선, 면이 가지는 특성을 예술적으로 접근하여 재해석함으로써 네일아트 표현에 대한 시각을 새롭게 모색하고 이를 활용하여 네일아트 디자인의 무한한 가능성을 높이는데 기여하고자 한다.

• 한국실내디자인학회논문집
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• 제33호
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• pp.122-128
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• 2002

In the Sci-Fi movie, with future scene in its setting, how images are shown in the film is very important. Sci-Fi movie is supposed to be expressed In different way of its set design. This study suggests various types of expression in Sci-Fi movies. Sci-Fi movies construct the narrative upon the binary opposition of nature vs. culture, human vs. nonhuman. Sci-Fi movie is a contested terrain where conservative, liberal and radical ideologies are competing. While liberal Sci-Fi movies consider the opposition compatible, conservative texts show the paranoid towards science and nonhumans. And, Sci-Fi movies are expressed as utopian and dystopian. Utopian and dystopian elements are found in the history of architecture. Utopian element is often found in the religious architecture, and those buildings are geometric, orderly, and gorgeous. Dystopian element is often found in modern architecture, and those are asymmetric, distorted, and incompleted. Conservative movie, which developing of science is expressed negatively, retains both utopian and dystopian elements evenly. Liberal movie, such as Space Opera which science is expressed positively, is changed from futurist and mechanical type into more simple, geometric and orderly shrine type. Radical movie, which the boundary of human and science is expressed darkly, retains dark, complex, and incomplete dystopian expression elements.

• 한국실내디자인학회논문집
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• 제40호
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• pp.68-75
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• 2003

The purpose of this study is to find out the expression style of space in Rietveld's early works in comparison with Mondrian's paintings. Through investigating the development of their works and analyzing the composition and disposition type of elements such as point, line, plane, color in the selected works, we can draw the following conclusions. .First, Rietveld pursues the dissolution of traditional volume and the de-composition of elements as Mondrian does. Second, the painterly property of plane is emphasized by the projection of separately floating elements on the picture plane. Third, the separating the layer of lines from the layer of planes and the re-combination of these two layers present the superimpositional image of three-dimensional objects. This leads into new conception of architectural space and develops the transparency and the extension of plan in modem architecture.

• 한국실내디자인학회논문집
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• 제21권5호
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• pp.135-144
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• 2012

Since Seoul was selected as the world design capital at the end of 2007, the endeavors to modernize the Korean unique beauty is becoming more tangible. Especially, the commercial spaces to be used by the general public and tourists have the value for study in the respect that such spaces for the national iconic images and have a large and far-reaching effect to transmit the national identity. Therefore, this study analyzed the design coordination properties in the Koreanity expression found in the modern commercial spaces. the foundation for the analysis was based on Leonard Koren's work on the arrrangement rhetoric theory, 'Arranging things(2003), and the contents of Korean expression were reviewed with professional jounals. the results are as follows: First, the design coordination properties in the Koreanity expression shown in modern commercial spaces are black-white contrast, natural materials, curved shapes, brevity, and simple beauty. Especially, some elements such as wood, traditional Korean papers, stones, mid-to-low chroma natural colors, checked patterns, and crazy patterns are used with overlapping and it is viewed that it is necessay to more aggressively seek for the disappeared traditional elements in the Korean modeling properties. Second, the concrete images of Korean expressions could be summarized as four adjective image groups, Natural, Subtitle, Gentle and Magnificent.