• Biomolecules & Therapeutics
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• v.16 no.1
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• pp.1-8
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• 2008

Mitochondria are important sensor of apoptosis. $H_2O_2-induced$ cell death rate was enhanced by serum deprivation. In this study, we investigated whether serum deprivation using 0.5 or 3 % FBS induces apoptotic cell death through mitochondrial enzyme activation as compared to 10 % FBS. Apoptotic cell death was observed by chromosome condensation and the increase of sub-G0/G1 population. Serum deprivation reduced cell growth rate, which was confirmed by the decrease of S-phase population in cell cycle. Serum deprivation significantly increased caspase-9 activity and cytochrome c release from mitochondria into cytosol. Serum deprivation-induced mitochondrial changes were also indicated by the increase of ROS production and the activation of mitochondrial enzyme, succinate dehydrogenase. Mitochondrial enzyme activity increased by serum deprivation was reduced by the treatment with rotenone, mitochondrial electron transport inhibitor. In conclusion, serum deprivation induced mitochondrial apoptotic cell death through the elevation of mitochondrial changes such as ROS production, cytochrome c release and caspase-9 activation. It suggests that drug sensitivity could be enhanced by the increase of mitochondrial enzyme activity in serum-deprived condition.

• Journal of the Microelectronics and Packaging Society
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• v.29 no.1
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• pp.71-75
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• 2022

Graphene oxide was stirred with a ZnCl₂:NaCl electrolyte and electrochemically coated by cyclic voltammetry to simplify the electron transpfer layer film forming process for organic solar cells and to fabricate an organic solar cell having it. The device structure is FTO/ZnO:graphene/P3HT:PCBM/PEDOT:PSS/Ag. Morphology and chemical properties of ETL were confirmed by scanning electron microscopy(SEM), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy. As a result of XPS measurement, ZnO metal oxide and carbon bonding were simultaneously confirmed, and ZnO and graphene peaks were confirmed by Raman spectroscopy. The electrical characteristics of the manufactured solar cell were specified with a solar simulator, and the ETL device coated twice at a rate of 0.05 V/s showed the highest photoelectric conversion efficiency of 1.94%.

• Journal of Korean Vacuum Science & Technology
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• v.4 no.3
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• pp.81-85
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• 2000

The effects of $NH_3$ flow rate and reactor pressure on Al composition and the interface of AlGaN/GaN heterostructure were studied. Equilibrium partial pressure of Ga and Al over AiGaN alloy was calculated as a function of growth pressure, $NH_3$flow rate and temperature. It was found equilbrium vapor pressure of Al is significantly lower than that of Ga, thus, the alloy composition mainly controlled by Ga partial pressure. We believe that more decomposition of Ga occur at lower $NH_3$ flow rate and higher growth pressure leads to preferred Al incorporation into AlGaN. The alloy composition gradient became larger at AlGaN/GaN heterointerface at higher reactor pressures, higher Al composition and low $NH_3$ flow rate. This composition gradient lowered sheet carrier concentration and electron mobility as well. We obtained an AlGaN/GaN heterostructure with sheet carrier density of ${\sim}2{\times}10^{13}cm^{-2}$ and mobility of 1250 and 5000 $cm^2$/Vs at 300 K and 100 K, respectively.

• Progress in Superconductivity and Cryogenics
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• v.9 no.4
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• pp.24-27
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• 2007

The purpose of this work is to develop, integrate, and implement an optical characterization method to evaluate physical properties in coated conductors and investigate the local distribution of the causes of degraded performance. The method that we selected at this moment is Raman scattering spectroscopy, which is accompanied with measurements of local supercurrent transport, phase composition, microstructure, and epitaxy quality for coated conductors that range in size up to multi-meter-length tapes and that embrace the entire tape embodiment (substrate through cap layer). The establishment of Raman spectroscopy as an on-line process monitoring tool is our eventual goal of research, but it requires very robust and cost-effective equipments. We analyzed $YBa_2Cu_3O_7(YBCO)$ thin films grown at various substrate temperatures by using Raman spectroscopy. YBCO films were grown by a high-rate electron-beam co-evaporation method. Raman spectra of YBCO films with lower-transport properties exhibit additional phonon modes at ${\sim}300cm^{-1}$, ${\sim}600cm^{-1}$ and ${\sim}630cm^{-1}$, which are related to second-phases such as $Ba_2Cu_3O_{5.9}$ and $BaCuO_2$. We propose a new method to characterize Raman spectra of coated conductors for an in-line quality control.

• Membrane Journal
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• v.24 no.5
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• pp.367-374
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• 2014

In the present study, disc-type LSCF/GDC (20 : 80 vol%) dual-phase membranes having porous LSC/GDC (50 : 50 vol%) active layers were prepared and effect of active layers on oxygen ion transport behavior was investigated. Introduction of active layers improved drastically oxygen flux due to enhanced electron conductivity and oxygen surface exchange activity. As firing temperature of active layer increased from $900^{\circ}C$ to $1000^{\circ}C$, oxygen flux increased due to improved contact between membrane and active layer or between grains of active layer. The enhanced contact would improve oxygen ion and electron transports from active layer to membrane. Also, as thickness of active layer increased from 10 to $20{\mu}m$, oxygen flux decreased since thick active layer rather prevented oxygen molecules diffusing through the pores. And, STF infiltration improved oxygen flux due to enhanced oxygen reduction reaction rate. The experimental data announces that coating and property control of active layer is an effective method to improve oxygen flux of dual-phase oxygen transport membrane.

• Bulletin of the Korean Chemical Society
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• v.32 no.11
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• pp.4035-4040
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• 2011

Long $TiO_2$ nanotube (NT) arrays, prepared by electrochemical anodization of Ti foils, have been utilized as dye-adsorbing electrodes in dye-sensitized solar cells (DSCs). By anodizing for 1-24 hr and subsequent annealing, highly crystallized and tightly-adhered NT arrays were tailored to 11-150 ${\mu}m$ lengths, ~90 nm innerpore diameter and ~30 nm wall thickness. I-V curves revealed that the photovoltaic conversion efficiency (${\eta}$) was proportional to the NT length up to 36 ${\mu}m$. Beyond this length, the ) was proportional to the NT length up to ${\eta}$ was still steadily increased, though at a much lower rate. For example, an ${\eta}$ of 5.05% at 36 ${\mu}m$ was increased to 6.18% at 150 ${\mu}m$. Transient photoelectron spectroscopic analyses indicated that NT array-based DSCs revealed considerably higher electron diffusion coefficient ($D_e$) and life time (${\tau}_e$) than those with $TiO_2$ nanoparticles (NP). Moreover, the electron diffusion lengths ($L_e$) of the photo-injected electrons were considerably larger than the corresponding NT lengths in all the cases, suggesting that electron transport in NT arrays is highly efficient, regardless of tube length.

• Journal of the Korean Institute of Telematics and Electronics
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• v.25 no.4
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• pp.422-429
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• 1988

The photoconductive multilayer of Se-As(hole blocking layer)/Se-As-Te (photoconductive layer) /Se-As (layer for supporiting hole transport)/Se-As(layer or controlling total capacitance)/Sb2S3(electron blocking layer) was fabricated and its electrical and optical properties were investigated. The photoconductive multilayer is made of evaporated a-Se as the base material, doped with As and Te to prevent the crystallization of a-Se and to enhance red sensitivity, respectively. The multilayer with good image reproducibility has the following deposition condition. The first layer has the thickness of 250\ulcornerat the deposition rate of 250\ulcornersec. The second layer has the thickness of 800\ulcornerat the deposition rate of 250\ulcornersec. The third layer has the thickness of 125\ulcornerat the deposition rate of 250\ulcornersec. The fourth layer has the thickness of 1700\ulcornerunder the Ar gas ambient of 50x10**-3torr. The image pick-up tube, employing this multilayer demonstrates the following characteristics. The photosensitivity is 0.8, the resolution limit is above 300TV line, and the decay lag is about 7%. And spectral response convers the whole visible range. Therfore the application to color TV camera is expected.

• Korean Journal of Microbiology
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• v.5 no.1
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• pp.15-19
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• 1967

Chlorella ellipsoidea cells were cultured in an iron, copper, zinc, manganese, molybdenum or boron-free medium. Physiological activities such as growth rate, reproduction, endogenous and glucose respiration, photosynthetic activity and biosythesis of chlorophyll of the micro-element definition cells were measured. It generally, growth rate, respiratory and photosynthetic activities, and biosynthesis of chlorophyll of the micro-element deficient cells decreased more or less, compared with those of the normal cells. The growth of the algal cells in an iron-free medium were retarded severely with the chlorosis, and the photosynthetic activity of the cells decreased remarkably even though the low content of chlorophyll in the cells owing to the iron-deficiency is considered. Therefore, it is deduced that iron takes part in the photosynthetic process itself, possibly by its participation in the photo phosphorylation coupled with electron transport. Respiratory activity of boron-deficient cells showed the most severe decrease whereas those of the molybdenum-deficient cells showed very slight decrease in spite of severe growth retardation.

• Korean Journal of Medicinal Crop Science
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• v.28 no.1
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• pp.1-8
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• 2020

Background: This study was conducted to investigate the effects of NaCl concentration on the photosynthetic parameters, chlorophyll fluorescence and growth characteristics of Crepidiastrum sonchifolium. Methods and Results: As treatments, we subjected C. sonchifolium plants to four different concentrations of NaCl (0, 50, 100 and 200 mM). We found that the photosynthetic parameters maximum photosynthesis rate (P_{N max}), net apparent quantum yield (Φ), maximum carboxylation rate (V_cmax), and maximum electron transport rate (J_max) were significantly reduced at an NaCl concentration greater than 100 mM. In contrast, there was an increase in water-use efficiency with increasing NaCl concentration, although in terms of growth performances, leaf dry weight, root dry weight, stem length, and total dry weight all decreased with increasing NaCl concentration. Furthermore, leakage of electrolytes, as a consequence of cell membrane damage, clearly increased in response to an increase in NaCl concentration. Analysis of the polyphasic elevation of chlorophyll a fluorescence transients (OKJIP) revealed marked decrease in flux ratios (Φ_PO, Ψ_O and Φ_EO) and the PI_abs, performance index in response to treatment with 200 mM NaCl, thereby reflectings the relatively reduced state of photosystem II. This increase in fluorescence could be due to a reduction in electron transport beyond Q^-_A. We thus found that the photosynthetic parameters, chlorophyll fluorescence and growth characteristics of C. sonchifolium significantly increased in response to treatment with 200 mM NaCl. Conclusions: Collectively, the findings of this study indicate that C. sonchifolium shows relatively low sensitivity to NaCl stress, although photosynthetic activity was markedly reduced in plants exposed to 200 mM NaCl.

• Journal of Environmental Science International
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• v.26 no.5
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• pp.601-608
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• 2017

The effects of elevated atmospheric $CO_2$ on growth and photosynthesis of soybean (Glycine max Merr.) were investigated to predict its productivity under elevated $CO_2$ levels in the future. Soybean grown for 6 weeks showed significant increase in vegetative growth, based on plant height, leaf characteristics (area, length, and width), and the SPAD-502 chlorophyll meter value (SPAD value) under elevated $CO_2$ conditions ($800{\mu}mol/mol$) compared to ambient $CO_2$ conditions ($400{\mu}mol/mol$). Under elevated $CO_2$ conditions, the photosynthetic rate (A) increased although photosystem II (PS II) photochemical activity ($F_v/F_m$) decreased. The maximum photosynthetic rate ($A_{max}$) was higher under elevated $CO_2$ conditions than under ambient $CO_2$ conditions, whereas the maximum electron transport rate ($J_{max}$) was lower under elevated $CO_2$ conditions compared to ambient $CO_2$ conditions. The optimal temperature for photosynthesis shifted significantly by approximately $3^{\circ}C$ under the elevated $CO_2$ conditions. With the increase in temperature, the photosynthetic rate increased below the optimal temperature (approximately $30^{\circ}C$) and decreased above the optimal temperature, whereas the dark respiration rate ($R_d$) increased continuously regardless of the optimal temperature. The difference in photosynthetic rate between ambient and elevated $CO_2$ conditions was greatest near the optimal temperature. These results indicate that future increases in $CO_2$ will increase productivity by increasing the photosynthetic rate, although it may cause damage to the PS II reaction center as suggested by decreases in $F_v/F_m$, in soybean.