• Journal of Korea Multimedia Society
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• v.19 no.8
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• pp.1619-1625
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• 2016

Recently, as the public interests about the 3D printing technology are increased, various kinds of 3D printers are being released. But, they are limited to use because they cannot fabricate an object which is larger than the printer's printing volume. To relieve this problem, we propose an interactive 3D mesh editing system for 3D printing the object that is larger than the printing volume. The proposed 3D editing system divides the input 3D mesh using the user's line drawings defining cutting planes and it attaches various connectors. The output meshes are guaranteed to fabricate without post-processing. The printed parts can be assembled using the connectors. Our proposed system has an advantage that it can be used easily by non-professional 3D printer users.

• Journal of Korean Medical Science
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• v.33 no.46
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• pp.287.1-287.14
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• 2018

Integrity of authorship and peer review practices are important considerations for ethical publishing. Criteria for authorship, as delineated in the guidelines by the International Committee of Medical Journal Editors (ICMJE), have undergone evolution over the decades, and now require fulfillment of four criteria, including the need to be able to take responsibility for all aspects of the manuscript in question. Although such updated authorship criteria were published nearly five years ago, still, many major medical and specialist journals have yet to revise their author instructions to conform to this. Inappropriate authorship practices may include gift, guest or ghost authorship. Existing literature suggests that such practices are still widely prevalent, especially in non-English speaking countries. Another emerging problem is that of peer review fraud, mostly by authors, but also rarely by handling editors. There is literature to suggest that a proportion of such fake peer review may be driven by the support of some unscrupulous external editing agencies. Such inappropriate practices with authorship malpractices or disagreement, or peer review fraud, have resulted in more than 600 retractions each, as identified on the retractions database of Retractionwatch.com. There is a need to generate greater awareness, especially in authors from non-English speaking regions of the world, about inappropriate authorship and unethical practices in peer review. Also, support of any external editing agency should be clearly disclosed by authors at the time of submission of a manuscript.

• Korean Journal of Agricultural Science
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• v.47 no.4
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• pp.903-920
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• 2020

Since its first demonstration as a practical genome editing tool in the early 2010s, the use of clustered regularly interspaced short palindromic repeat (CRISPR) along with the endonuclease Cas9 (CRISPR/Cas9) has become an essential choice for generating targeted mutations. Due to its relative simplicity and cost-effectiveness compared to other molecular scissors, i.e., zinc finger nuclease (ZFN) and transcription activator-like effector nuclease (TALEN), the CRISPR/Cas9 system has been shown to have a massive influence on genetic studies regardless of the biological kingdom. Although the system is in the process of being established, numerous protocols have already been released for the system and there have been various topics of CRISPR related papers published each year in ever-increasing manner. Here, we will briefly introduce CRISPR/Cas9 system and discuss the variants of the CRISPR system. Also, their applications to crop improvement will be dealt with mainly ornamental crops among horticultural crops other than Arabidopsis as a model plant. Finally, some issues on the barriers restraining the use of CRISPR system on floricultural crops, the prospect of CRISPR system as a DNA-free genome editing tool with efficient facilitators and finally, the future perspectives on the CRISPR system will be described.

• Journal of the Korean Society of Clothing and Textiles
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• v.45 no.3
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• pp.510-524
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• 2021

Selfie practices on social media can result in negative body image for men. The current study investigated the dual body image pathway model for the relationship between selfie practices on social media and body satisfaction, with internalization as a mediator. Structural equation modeling analyses supported our research model when studying 446 young Korean male Instagram users. The results indicated that selfie editing behavior, but not selfie browsing behavior, significantly predicted an increased internalization of a body ideal. The positive associations between internalization, muscularity dissatisfaction, and body fat dissatisfaction were confirmed. Additionally, the indirect effects of selfie editing behavior on muscularity and body fat dissatisfaction due to internalization were significant. The study confirmed the detrimental effects of selfie behavior on body satisfaction for male social media users. The results provided valuable information that selfie editing may be a risky behavior since it can result in developing muscularity and body fat dissatisfaction. Theoretical contributions and practical implications were discussed. Future research should address the age and cultural differences that may elucidate the impacts of selfie practices on men's body image concerns.

• Mycobiology
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• v.49 no.6
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• pp.599-603
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• 2021

CRISPR/Cas9 genome editing systems have been established in a broad range of eukaryotic species. Herein, we report the first method for genetic engineering in pyogo (shiitake) mushrooms (Lentinula edodes) using CRISPR/Cas9. For in vivo expression of guide RNAs (gRNAs) targeting the mating-type gene HD1 (LeA1), we identified an endogenous LeU6 promoter in the L. edodes genome. We constructed a plasmid containing the LeU6 and glyceraldehyde-3-phosphate dehydrogenase (LeGPD) promoters to express the Cas9 protein. Among the eight gRNAs we tested, three successfully disrupted the LeA1 locus. Although the CRISPR-Cas9-induced alleles did not affect mating with compatible monokaryotic strains, disruption of the transcription levels of the downstream genes of LeHD1 and LeHD2 was detected. Based on this result, we present the first report of a simple and powerful genetic manipulation tool using the CRISPR/Cas9 toolbox for the scientifically and industrially important edible mushroom, L. edodes.

• International journal of advanced smart convergence
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• v.12 no.4
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• pp.182-189
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• 2023

A recently research, object detection and segmentation have emerged as crucial technologies widely utilized in various fields such as autonomous driving systems, surveillance and image editing. This paper proposes a program that utilizes the QT framework to perform real-time object detection and precise instance segmentation by integrating YOLO(You Only Look Once) and Mask R CNN. This system provides users with a diverse image editing environment, offering features such as selecting specific modes, drawing masks, inspecting detailed image information and employing various image processing techniques, including those based on deep learning. The program advantage the efficiency of YOLO to enable fast and accurate object detection, providing information about bounding boxes. Additionally, it performs precise segmentation using the functionalities of Mask R CNN, allowing users to accurately distinguish and edit objects within images. The QT interface ensures an intuitive and user-friendly environment for program control and enhancing accessibility. Through experiments and evaluations, our proposed system has been demonstrated to be effective in various scenarios. This program provides convenience and powerful image processing and editing capabilities to both beginners and experts, smoothly integrating computer vision technology. This paper contributes to the growth of the computer vision application field and showing the potential to integrate various image processing algorithms on a user-friendly platform

• International Journal of Stem Cells
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• v.16 no.2
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• pp.234-243
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• 2023

The recent advances in human pluripotent stem cells (hPSCs) enable to precisely edit the desired bases in hPSCs to be used for the establishment of isogenic disease models and autologous ex vivo cell therapy. The knock-in approach based on the homologous directed repair with Cas9 endonuclease, causing DNA double-strand breaks (DSBs), produces not only insertion and deletion (indel) mutations but also deleterious large deletions. On the contrary, due to the lack of Cas9 endonuclease activity, base editors (BEs) such as adenine base editor (ABE) and cytosine base editor (CBE) allow precise base substitution by conjugated deaminase activity, free from DSB formation. Despite the limitation of BEs in transition substitution, precise base editing by BEs with no massive off-targets is suggested to be a prospective alternative in hPSCs for clinical applications. Considering the unique cellular characteristics of hPSCs, a few points should be considered. Herein, we describe an updated and optimized protocol for base editing in hPSCs. We also describe an improved methodology for CBE-based C to T substitutions, which are generally lower than A to G substitutions in hPSCs.

• 한국HCI학회:학술대회논문집
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• 2007.02a
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• pp.243-249
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• 2007

In this paper we introduce a system that assists news reporters to easily edit source video anywhere, and transfer the result from a remote site to the station. Presently reporters are bothered to edit source materials, compress and transfer using several separate tools. Moreover such tools have lots of too complicated features to use only for news production. We have integrated file sending and video editing functions together for seamless workflow. News programs usually need promptness in production. For this reason our system is designed to make it possible the one-stop production of news items outside the TV station. Also the system reflects the demands of field workers, that is, easy usage for the beginner and features for speedy news production. One of the most focused is that the reliability of the system is guaranteed in the process of transfer. To achieve this end, we have implemented several error-resilient schemes for unexpected network errors. Our system consists of remote laptop editing terminals and a newsgathering server. DV-format based editing and analog-to-DV converting device are adopted to capture video from not only lightweight DV camcorders but also old-fashioned ENG cameras into laptop computers. We expect that news reporters and cameramen can take terminals anywhere the Internet is available and both editing and sending will be done on the spot. This will help increase the competitive power of our news production.

• Trans-
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• v.13
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• pp.59-90
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• 2022

Short-form content, which means "short video content within 10 minutes," is rapidly emerging as a recent trend among MZ generations based on the fact that it can be viewed whenever there is a short running time, and they are physically short, colorful, and deliver a lot of information in a compressed time, showing differences in both long-form content and format. In addition, entertainment videos such as information delivery videos, eating shows, web entertainment, and dance challenges are mainly produced and distributed, so there is no need to take expertise as a creative work of video experts, and consumers often become producers by directly participating in production using low-end equipment such as smartphones. For these reasons, shortform content creates new image styles rather than general existing image forms such as long-form contents, and this study focuses on changes in editing styles. This study summarized the following five characteristic changes by analyzing the editing style of short form content that has changed compared to long form content according to the 'visual' aspect. The use of frames, memes, screen division, blue screen, and subtitles are included, and by organizing each characteristic, we identified the editing style of shortform content that has emerged as a recent trend and learned about the changes.

• Journal of Mushroom
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• v.20 no.3
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• pp.178-182
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• 2022

Despite the long history of mushroom use, studies examining the genetic function of mushrooms and the development of new varieties via bio-molecular methods are significantly lacking compared to those examining other organisms. However, owing to recent developments, attempts have been made to use a novel gene-editing technique involving CRISPR/Cas9 technology and genetic scissors in mushroom studies. In particular, research is actively being conducted to utilize ribonucleoprotein particles (RNPs) that can be genetically edited with high efficiency without foreign gene insertion for ease of selection. However, RNPs are too large for Cas9 protein to pass through the cell membrane of the protoplasmic reticulum. Furthermore, guide RNA is unstable and can be easily decomposed, which remarkably affects gene editing efficiency. In this study, nanoparticles were used to mitigate the shortcomings of RNP-based gene editing techniques and to obtain transformants stably. We used Lentinula edodes (shiitake mushroom) Sanjo705-13 monokaryon strain, which has been successfully used in previous genome editing experiments. To identify a suitable osmotic buffer for the isolation of protoplast, 0.6 M and 1.2 M sucrose, mannitol, sorbitol, and KCl were treated, respectively. In addition, with various nanoparticle-forming materials, experiments were conducted to confirm genome editing efficiency via the formation of nanoparticles with calcium phosphate (CaP), which can be bound to Cas9 protein without any additional amino acid modification. RNPs/NP complex was successfully formed and protected nuclease activity with nucleotide sequence specificity.