• Journal of Plant Biotechnology
• /
• v.4 no.4
• /
• pp.155-161
• /
• 2002

The HCV gene was expressed in potato plants under the control of the constitutive CaMV 355 promoter or tuber-specific patatin promoter. Solanum tuberosum plants carrying a plant expression vector harboring the encoding region of HCV gene were generated by Agrobacterium tumefaciens-mediated in vitro transformation methods. The presence of HCV gene in the plant genome was detected by PCR and DNA hybridization experiments. We obtained the 5 lines of transgenic potato with the pMBPHCV construct and 4 lines of transgenic potato with the pATHCV construct. The HCV transgenic stably integrated into the potato genome, as well as their transcription. HCV mRNA was identified in leaf and tuber tissues of transgenic plants by Northern blot analysis. The transgenic potato plants produced the expected transcript, and the corresponding HCV protein accumulated in individual transgenic plants.

• Journal of Haehwa Medicine
• /
• v.22 no.2
• /
• pp.23-35
• /
• 2014

Gu-Hwang Plants(plants collected from mountains and fields and eaten when no food was available) is healthy, life saving food material, being Mi(taste) and Ki(energy) of nature, for modern people who lose their health by oversupplying energy and unbalanced diet. Also, Gu-Hwang Plants is material for new medicine and functional foods. However, there is a lack of document research about Gu-Hwang plants in Korea. "Gu-Hwang-Bon-Cho" is a book about plants written as a guide for collecting food from nature and maintaining life at times when no food is available due to natural disaster. The book describes in which soils the plants are produced and the names for the plants, and classifies them according to hot and cold characters and sweet and bitter tastes of the plants. In addition, the book distinguished plants' edible parts, such as flower, fruit, root, stem, peel, and leaf, with how to collect and cook them. The book is of great value that it is reprinted over generations and now there are 30 kinds of existing books. This study conducted research based on books published throughout history which are the first book of this kind by Ju-wang-ju, a book published in 1525 by I-Ryeom, a book published in 1555 by Youk-gan, a book published by Ho-seung of Sa-cheon, and a book published in 1566 by Ju-gon.

• Natural Product Sciences
• /
• v.14 no.1
• /
• pp.62-67
• /
• 2008

The leaf extract of Ligularia stenocephala showed the highest anti-platelet aggregating activity in large numbers of edible and herbal plants. The active fraction fractionationed from L. stenocephala extract by using preparative HPLC inhibited the platelet aggregation up to above 80% and its blood coagulating time (PT and APTT) also showed similar effect to aspirin (0.2 ${\mu}g/mL$), known as an anti-thrombus compound. Two antithrombus active compounds were purified and identified as 3,5-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid, respectively, on the basis of NMR and FAB-MS spectroscopic data. Two active compounds has not only antiplatelet aggregating activity, but also has anticoagulating activity.

• Mycobiology
• /
• v.43 no.3
• /
• pp.311-318
• /
• 2015

Culture filtrates of six different edible mushroom species were screened for antimicrobial activity against tomato wilt bacteria Ralstonia solanacearum B3. Hericium erinaceus, Lentinula edodes (Sanjo 701), Grifola frondosa, and Hypsizygus marmoreus showed antibacterial activity against the bacteria. Water, n-butanol, and ethyl acetate extracts of spent mushroom substrate (SMS) of H. erinaceus exhibited high antibacterial activity against different phytopathogenic bacteria: Pectobacterium carotovorum subsp. carotovorum, Agrobacterium tumefaciens, R. solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, X. axonopodis pv. vesicatoria, X. axonopodis pv. citiri, and X. axonopodis pv. glycine. Quantitative real-time PCR revealed that water extracts of SMS (WESMS) of H. erinaceus induced expressions of plant defense genes encoding ${\beta}$-1,3-glucanase (GluA) and pathogenesis-related protein-1a (PR-1a), associated with systemic acquired resistance. Furthermore, WESMS also suppressed tomato wilt disease caused by R. solanacearum by 85% in seedlings and promoted growth (height, leaf number, and fresh weight of the root and shoot) of tomato plants. These findings suggest the WESMS of H. erinaceus has the potential to suppress bacterial wilt disease of tomato through multiple effects including antibacterial activity, plant growth promotion, and defense gene induction.

• Mycobiology
• /
• v.38 no.2
• /
• pp.97-101
• /
• 2010

Calocybe indica, a tropical edible mushroom, is popular because it has good nutritive value and it can be cultivated commercially. The current investigation was undertaken to determine a suitable substrate and the appropriate thickness of casing materials for the cultivation of C. indica. Optimum mycelial growth was observed in coconut coir substrate. Primordia initiation with the different substrates and casing materials was observed between the 13th and 19th day. The maximum length of stalk was recorded from sugarcane leaf, while diameter of stalk and pileus, and thickness of pileus were found in rice straw substrate. The highest biological and economic yield, and biological efficiency were also obtained in the rice straw substrate. Cow dung and loamy soil, farm-yard manure, loamy soil and sand, and spent oyster mushroom substrates were used as casing materials to evaluate the yield and yield-contributing characteristics of C. indica. The results indicate that the number of effective fruiting bodies, the biological and economic yield, and the biological efficiency were statistically similar all of the casing materials used. The maximum biological efficiency was found in the cow dung and loamy soil casing material. The cow dung and loamy soil (3 cm thick) was the best casing material and the rice straw was the best substrate for the commercial cultivation of C. indica.

• Korean Journal of Pharmacognosy
• /
• v.45 no.3
• /
• pp.220-226
• /
• 2014

Diospyros lotus has been cultivated for its edible fruits and leaves which are considered for its medicinal importance. The aim of this study was to evaluate the anti-melanogenesis of ethyl acetate (EA) fractions from D. lotus leaves in B16 cells. The order of the total polyphenol content with regard to the different solvent fractions from D. lotus leaves was EA>butanol>metahanol>chloroform>n-hexane. The major compounds of EA fraction from D. lotus leaves by HPLC analysis were myricitrin and myricetin. Cellular TYR activity and melanin content in response to treatment with 100 mg/mL of EA fraction was inhibited more strongly than group treated with arbutin. Further, EA fraction exhibited significant anti-melanogenesis effects by reducing the levels of microphthalima-associated transcription factor (MITE), inhibiting the synthesis of TYR, tyrosinase-related protein-1 (TRP-1) and TRP-2. Therefore, EA fractions from D. lotus leaves may be a good source of skin-whitening agents in the future development of medicine-based trouble skin therapy.

• Journal of Pharmacopuncture
• /
• v.26 no.1
• /
• pp.10-17
• /
• 2023

Objectives: Murraya paniculata (family-Rutaceae), popularly known as orange jasmine, is the most important evergreen plant. The Rutaceae family is economically significant due to its diverse edible fruits and essential oils. Methods: Murraya paniculata extracts (MPE) of leaf have been shown to include phenolic compounds, highly oxygenated flavonoids, flavanones, sesquiterpenoids, polymethoxy glycosides, and coumarins. Cyclocitral, methyl salicylate, trans-nerolidol, cubenol, isogermacrene, -cadinol, and cubeb-11-ene are all abundant in MPE. The usages of various parts of this plant, such as bark, leaves and flower, as a remedy for a variety of ailments as widely recorded in the traditional literature. The plant has anti-diabetic, anti-obesity, antibacterial, anti-implantation, anti-oxidative, cytotoxic, anti-diarrheal, antidepressant and anti-anxiety properties and many others. Results: The goal of the review is to reignite interest in this potential plant, encouraging researchers to continue their research in order to uncover novel therapeutic compounds for the treatment and management of a range of infections. The current review provided a comprehensive overview of this traditional unique plant. Conclusion: The review paves a way for exploring its active chemical elements with substantial pharmacological values further for potential benefits of mankind.

• Journal of Korean Society of Forest Science
• /
• v.106 no.4
• /
• pp.388-396
• /
• 2017

This study was carried out to determine effects of bamboo species on the distribution of nutrients in aboveground biomass of Phyllostachys bambusoides and Phyllostachys nigra var. nenosis, Damyanggun, Korea. The study site was established around 40-year-ago to produce bamboo culm and edible shoot production. Total 28 bamboos (14 P. bambusoides and 14 P. nigra var. nenosis) were cut to measure nutrient concentration of each bamboo component, such as culm, branches and leaf. Magnesium concentration in each bamboo component was significantly higher in the P. bambusoides than in the P. nigra var. nenosis. Nutrient concentrations except for calcium were significantly higher in the current-year-old bamboos than in the > 1-year-old bamboos. The nutrient concentration in leaf was generally highest in carbon, followed by potassium or nitrogen, phosphorus, calcium or magnesium. Total nutrient content in each bamboo component was significantly higher in the P. bambusoides than in the P. nigra var. nenosis. The nutrient content of bamboo biomass was the highest in carbon, followed by potassium, nitrogen, phosphorus, magnesium and calcium. The results indicate that P. bambusoides uptakes more nutrients compared with P. nigra var. nenosis during growth development.

• Journal of Bio-Environment Control
• /
• v.16 no.4
• /
• pp.388-394
• /
• 2007

To product the edible fresh shoots of Sedum sarmentosum in summer season, 4 local strains were cultivated with cutting propagation under 0, 30, 50, 70, 90% shading. Effect of shading on shoot growth, leaf chroma value, SPAD value, and bitterness were investigated at 35 days after cutting. Plant height, the number of node and leaf stem diameter in $30{\sim}90%$ shading were increased than in the control, and Pohang local strain had little-overgrowth shoot, thicker stem, a few brenches per shoot, and larger leaf compared to the other local strains. Fresh and dry weight under shading were increased than in the control, dry weight of pohang local strain was highest in 50% shading. Hunter L and b values were decreased out of proportion to higher shading rate. SPAD value in $50{\sim}90%$ shading was significantly lower than in the control. The bitterness of fresh shoot was decreased out of proportion to higher shading rate. In Summer season, producing the fresh shoot of S. sarmentosum was excellent in 50% shading, and Pohang local strain among 4 local strains was more stable and good in yield and quality under shading.

• Journal of Life Science
• /
• v.29 no.9
• /
• pp.977-985
• /
• 2019

Aster yomena (Kitam.) Honda is an edible vegetable and perennial herb belonging to the Asteraceae family, and has been used for a long time for the prevention and treatment of various diseases. Although leaf extracts of A. yomena are known to have antioxidant and anti-inflammatory effects, accurate efficacy assessments are still inadequate. In this study, we investigated whether the antioxidant efficacy of ethanol extract of A. yomena leaf (EEAY) is correlated with the anti-inflammatory effect in RAW 264.7 macrophages. The results showed that EEAY significantly inhibited the hydrogen peroxide ($H_2O_2$)-induced growth inhibition in RAW 264.7 cells, which was associated with increased expression of nuclear factor erythroid 2-related factor-2 (Nrf2) and heme oxygenase-1 (HO-1). EEAY pretreatment also effectively prevented $H_2O_2$-induced reactive oxygen species generation and apoptosis through inhibition of caspase-3 activation and poly (ADP-ribose) polymerase degradation. Additionally, EEAY significantly increased the expression and production of interleukin-10, a representative anti-inflammatory cytokine, which was associated with increased expression of toll-like receptor 4 and myeloid differentiation factor 88 at transcriptional and translational levels. Furthermore, the increased production of nitric oxide (NO) by lipopolysaccharide was markedly abolished under the condition of EEAY pretreatment, and the inhibitory effect of NO production by EEAY was further increased by hemin, an HO-1 inducer. Overall, our results suggest that EEAY is able to activate the Nrf2/HO-1 signaling pathway to protect RAW 264.7 macrophages from oxidative and inflammatory stress.