Browse > Article

Antithrombotic Compounds form the Leaves of Ligularia stenocephala M.  

Yoon, Min-Ho (Department of Bio Environmental Chemistry, College of Agriculture, Chungnam National University)
Cho, Chong-Woon (Department of Bio Environmental Chemistry, College of Agriculture, Chungnam National University)
Lee, Jae-Won (Department of Bio Environmental Chemistry, College of Agriculture, Chungnam National University)
Kim, Young-Shin (Department of Bio Environmental Chemistry, College of Agriculture, Chungnam National University)
An, Gil-Hwan (Department of Food Science and Technology, College of Agriculture, Chungnam National University)
Lim, Chi-Hwan (Department of Bio Environmental Chemistry, College of Agriculture, Chungnam National University)
Publication Information
Natural Product Sciences / v.14, no.1, 2008 , pp. 62-67 More about this Journal
Abstract
The leaf extract of Ligularia stenocephala showed the highest anti-platelet aggregating activity in large numbers of edible and herbal plants. The active fraction fractionationed from L. stenocephala extract by using preparative HPLC inhibited the platelet aggregation up to above 80% and its blood coagulating time (PT and APTT) also showed similar effect to aspirin (0.2 ${\mu}g/mL$), known as an anti-thrombus compound. Two antithrombus active compounds were purified and identified as 3,5-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid, respectively, on the basis of NMR and FAB-MS spectroscopic data. Two active compounds has not only antiplatelet aggregating activity, but also has anticoagulating activity.
Keywords
Ligularia stenocephala M.; 3,4-dicaffeoylquinic acid; 3,5-dicaffeoylquinic acid; antithrombus activity;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By SCOPUS : 4
연도 인용수 순위
1 Hara, H., Kitajima, A., Shimada, H., and Tamao, Y., Antithrombotic effect of MCI-9024, a new antiplatelet agent experimental thrombosis models. Thromb. Haemorr. 66, 484 (1991)
2 Hur, J.Y., Lee, P.J., Kim, H.C., Kang, I.S., Lee, K.R., and Kim, S,Y., (-)- 3,5-Dicaffeoyl-muco-quinic acid isolated from Aster scaber contributes to the differentiation of PC12 cells: through tyrosine kinase cascade signaling. Biochemical and biophysical research communications 313, 948-953 (2004)   DOI   ScienceOn
3 Iwai, K., Kishimoto, N., Kakino, Y., Mochida, K., and Fujita, T., In vitro antioxidative effects and tyrosinase inhibitory activities of seven hydroxycinnamoyl derivatives in green coffee beans. Journal of Agricultural and Food Chemistry 52, 4893-4898 (2004)   DOI   ScienceOn
4 Robinson, W.E., Cordeiro, M, Abdel-Malek, S., Jia, Q., Chow, S.A., Reinecke, M.G., and Mitchell, W.M, Dicaffeoylquinic acid inhibitors of human immunodeficiency virus integrase: inhibition of the core catalytic domain of human immunodeficiency virus integrase. Molecular pharmacology 50, 846-855 (1996)   PUBMED
5 Wang, S.Y., Chang, H.N., Lin, K.T., Lo, C.P., Yang, N.S., and Shyur, L.F.,Antioxidant properties and phytochemical characteristics of extracts from Lactuca indica. Journal of Agricultural and Food Chemistry 51, 1506-1512 (2003)   DOI   ScienceOn
6 Yun-Choi, H.S., Chung, K.S., Lim, M.H., and Oh, J.H., Antithrombotic effects of some traditional plant medicines. Kor. J. Pharmacogn. 26, 154-158 (1995)   과학기술학회마을
7 Yun-Choi, H.S., Kim, S.O., Kim, J.H., Lee, J.R., and Cho, H.I., Modified smear method for screening potential inhibitors of platelet aggregation from plant sources, J. Nat. Prod. 48, 363 (1985)   DOI
8 Choi, J.W., Park, K., Lee, K.T., Park, K.K., Kim, W.B., Lee, J.H., Jung, H.J., and Park, H.J., Inhibitory effect of Ligularia fischeri var. spiciformis and its active component, 3,4-dicaffeoylquinic acid on the hepatic lipid peroxidation in acetaminophen-treated rat. Natural Product Sciences 10, 182-189 (2004)
9 Kim, C.M., Kwon, Y.S., and Yun-Choi, H.S., Antithrombotic effect of BuOH soluble fraction of Angelica dahurica root. Kor. J. Pharmacogn. 26, 74-77 (1995)   과학기술학회마을
10 Hsu, H., Chen, Y.P., Shen, S.J., Hsu, C.S., Chen, C.C., and Chang, H.C., Oriental Materia Medica, a Concise Guide, pp 503-04. Oriental Healing Arts Institute, Taiwan, 1986
11 Makoto, N., Ruriko, I., Ko, K., Yasuko, K., and Yasuo, F., 5-lipoxy inhibitors from Gardeniae fructus. Chem. Pharm. Bull. 36, 87-95 (1988)   DOI   PUBMED   ScienceOn
12 Lee, S.E., Chung, T.Y., Seong, N.S., and Bang, J.K., In vitro antioxidant activity of 3,5- dicaffeoylquinic acid isolated from Aster scaber Thumb. Agricultural Chemistry and Biotechnology 47, 27-30 (2004)
13 Nishizawa, M., Izuhara, P., Kaneko, K., Koshihara, Y., and Fujimoto, Y. 5-Lipoxygenase inhibitors isolated from Gardeniae fructus. Chem.Pharm. Bull. 35, 87-95 (1988)
14 Heilmann, J., Merfort, I., and Weiss, M., Radical scavenger activity of different 3',4'-dihydroxyflavonols and 1,5-dicaffeoylquinic acid studied by inhibition of chemiluminescence. Planta Medica 61, 435- 438 (1995)   DOI   ScienceOn
15 Kim, H.J., Jin, C.B., and Lee, Y.S., Isolation and antioxidative activities of caffeoylquinic acid derivatives and flavonoid glycosides from leaves of sweet potato (Ipomoea batatas L.). Journal of Applied Pharmacology 15, 46-51 (2007)   DOI   ScienceOn
16 Paik, Y.S., Song, C.H., Chung, K.S., and Yun-Choi, H.S., Anti-platelet and anti-thrombotic effects of Gastrodia elata. Kor. J. Pharmacogn. 26(4), 385-389 (1995)
17 Kim, S.S., Park, R.Y., Jeon, H.J., Kwon, Y.S., and Chun, W.J., Neuroprotective effects of 3,5-dicaffeoylquinic acid on hydrogen peroxide-induced cell death in SH-SY5Y cells. Phytotherapy Research 19, 243-245 (2005)   DOI   ScienceOn
18 Yoon, M.H., Lim, C.H., Oh, J.H., Lee, J.C., and Choi, W.Y., Screening of Inhibitors of platelet aggregation from edible plants. Jour. Agri. Sci. Chungnam Nat'l Univ. 24, 267-274 (1997)
19 Terashima, S., Shimizu, M., Horie, S., and Morita, N., Studies on aldose reductase inhibitors from natural products. IV. Constituents and aldose reductase inhibitory effect of Chrysanthemum morifolium, Bixa orellana and Ipomoea batatas. Chem. Pharm. Bull. 39, 3346-3347 (1991)   DOI   PUBMED   ScienceOn