• 한국미생물·생명공학회지
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• 제37권4호
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• pp.399-404
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• 2009

재조합 대장균으로부터 회수된 고순도 베타카로틴을 첨가한 기능성 화장품의 제조방법을 개발하고 이의 임상실험을 수행하여 개발된 화장품의 주름개선 성능을 평가하였다. 베타카로틴의 유효성, 각종 피부흡수 및 사용성, 질감 등을 고려하여 화장품을 처방하였다. 베타카로틴의 첨가량은 피부에 독성을 나타내지 않고 콜라겐생합성 촉진효과가 우수하며 베타카로틴 자체의 특유한 붉은 색소가 피부에 묻어나오지 않을 정도인 화장품 kg 당 1 mg으로 결정하였다. 베타카로틴을 용해시키기 위하여 식용오일을 사용하였고 항산화물질인 vitamin E(tocopheryl acetate)를 첨가함으로써 제품의 장기간 보관 시 베타카로틴의 안정성과 유효성에 도움을 주게 하였다. 유화제 및 유화안정제는 2.0% 첨가함으로써 유상과 수상이 분리되지 않도록 하였다. 유연제인 dimethicone을 0.35% 첨가함으로써 백탁 현상을 제거하고 피부에 대한 사용감을 좋게 하였다. 보습작용을 보이는 sorbitol을 첨가함으로써, 건조한 환경에 대한 피부트러블을 최소화하게 하였다. 제품의 안정도를 조사하였다. 60일간의 실험에서 상온에 보관된 제품의 pH는 변화가 거의 없었고 0, 25, $40^{\circ}C$의 온도서 물리 화학적 변화가 없는 안정한 상태를 유지하였다. 뿐만 아니라 광제품의 주름개선 성능을 평가하기 위하여 20명의 피시험자를 대상으로 화장품의 인체적용시험을 실시하였다. 시험자의 평가결과 4주 후 피험자의 38%가 주름이 감소하였고 8주 후에는 68%가 주름이 개선되었다.

• 한국잠사곤충학회지
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• 제53권1호
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• pp.38-43
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• 2015

양잠농가의 소득증대 및 산업기반 확대를 위한 새로운 형태의 고 부가가치 양잠산물 창출을 통하여 현재까지 섭취가 어려웠던 5령4일부터 숙잠기 누에의 식용화를 위한 가공기술을 개발하였다. 농민들도 쉽게 가공이 가능하도록 생누에를 열수에 침지하여 삶거나 수증기로 쪄서 익혀 본 결과 누에의 몸체와 더불어 누에체내에 들어 있는 견사선의 섭취가 가능함은 물론 맛도 좋은 것으로 나타났다. 상기 기술은 원천기술로서 향후 일반식품, 건강기능식품, 화장품, 애완동물용 사료 등으로의 광범위한 활용이 예상돼, 양잠농가 소득증대와 산업기반 확대에 큰 기여를 할 것으로 기대된다.

• 대한화장품학회지
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• 제44권3호
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• pp.277-284
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• 2018

콜라겐 펩타이드(collagen peptide)는 단백질의 가수 분해물로서 주름 완화, 보습력 증대, 탄력 개선 등의 특정 피부 효능을 나타낼 수 있어 화장품 혹은 피부 개선 기능식품으로서 활용되고 있다. ${\gamma}-aminobutyric$ acid (GABA)는 척추 동물의 뇌, 척수에 존재하는 신경전달 물질로서 수면의 질과 양을 개선해 준다고 알려져 있다. 본 연구에서는 콜라겐 펩타이드와 GABA의 복합물이 수면 장애가 있는 여성에게 8주 경구 섭취를 통해서 수면 및 피부 상태를 개선할 수 있는가에 관하여 확인하였다. 복합물(J85091900)은 8주간 연속적으로 섭취 시, 수면장애지수(PSQI)가 유의적으로 감소하였으며, 수면 시간을 7% 증가시켰다. 또한, 피부 거칠기, 눈가 주름 및 피부 수분량(capacitance)을 유의적으로 개선하였다. 이상의 결과에서 콜라겐과 GABA의 복합물은 복합 수면 장애에 따른 피부의 노화 현상으로부터 피부를 보호할 수 있음을 확인하여 먹는 화장품의 핵심 소재로 활용 가능함을 확인하였다.

• 한국수산과학회지
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• 제54권3호
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• pp.311-317
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• 2021

Growth differences in Caulerpa okamurae thallus parts and the effects of apex removal on growth were examined. Erect fronds, stolons, and erect fronds with stolons (erect+stolon) were grown for 14 days, whereas erect fronds without and with apex removal (-A and +A fronds, respectively), were grown for 25 days. The relative growth rates (RGR) of C. okamurae thallus parts ranged from 1.91% to 4.93% day^-1 with maximal and minimal RGR for the stolon and erect+stolon treatments, respectively. Over the 25 days in culture, the -A fronds showed a higher RGR (2.48% day^-1) than the +A fronds (1.96% day^-1). More new erect fronds and stolons were produced by -A fronds than those by +A fronds. In addition, basal cutting of erect C. okamurae fronds resulted in a greater plant length increase than that obtained from top cutting. These findings indicate that vegetative growth is common in C. okamurae, which has excellent wound healing ability, and cutting damage to erect fronds may stimulate enhanced vegetative growth. Thus, we suggest that stolons and erect fronds of C. okamurae could be used as seeding materials for large scale cultivation. Furthermore, this edible species could be a potential seaweed resource for rapid skin regeneration cosmetics.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권1호
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• pp.1-4
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• 2003

Sericulture is a traditional agro-industry, which involves mulberry cultivation and silkworm rearing, has made great contributes to the human civilization. With the development of national economy and modem technology, mulberry and silkworm are being used to develop products with functionality besides the traditional cocoon production in China. In this paper, we brief the current developing situation of sericultural byproducts with functionality in the following aspects. (1) Functional products from silkworm larvae: silkworm powder, white muscardine silkworm, isolation and purification of anti-bacterial proteins from the larvae and production of medically valuable substances by Bombyx mori nuclear polyhedrosis virus (BmNPV) vector. (2) Utilization of silkworm feces: for pillow and for isolation of chlorophyll etc. (3) Production of valuable Chinese traditional medicine like Cordyceps sinensis with pupae, functional utilization of pupa protein and chitin. (4) Silk as additives to cosmetics, silk food and medical materials. (5) Functional utilization of mulberry: cultivation of edible fungus on mulberry shoots as medium, mulberry fruit drinks, mulberry tea, etc. The prospect of sericultural byproduct industry in China is also discussed.

• Natural Product Sciences
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• 제27권4호
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• pp.300-306
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• 2021

Chronic ultraviolet (UV) radiation causes photoaging, which represents skin damage, disrupts skin barrier function, and promotes wrinkle formation. We investigated that the polysaccharide extract of an edible basidiomycetous white jelly mushroom, Tremella fuciformis, (TF-Glucan^®) exhibited statistically photoprotective activity by inhibiting matrix metalloproteases (MMPs) and increasing collagen synthesis, and an anti-inflammatory activity by inhibiting nitric oxide and pro-inflammatory cytokines at the concentrations of less than 1000 ㎍/ml, which is not cytotoxic (p < 0.05). Additionally, TF-Glucan^® increased the expression of involucrin and filaggrin to prevent the disruption of UVB-induced barrier function (p < 0.05). TF-Glucan^® was assessed as a safe material by the human primary skin irritation (1, 3, 5%), human repeated insult patch test (no sensitization at 5%), 3T3 NRU phototoxicity assay (no phototoxicity, PIF < 2, MPE < 0.1), eye irritation test test by BCOP (no category, IVIS ≤ 3) and local lymph node assay (negative at 10, 25, 50%) for identifying potential skin sensitizing. These results suggest that TF-Glucan^® may be useful as an anti-photoaging ingredient for developing cosmeceuticals.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.436-446
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• 2023

Since Stewartia koreana leaves are registered with the Food and Drug Administration as edible herbal materials, they are used in the development of functional foods, cosmetics, and medicines. In this study, we established an analysis method that can simultaneously analyze two indicators, hyperoside (quercetin 3-O-galactoside) and isoquercitrin (quercetin 3-O-glucoside) contained in the leaves of S. koreana using HPLC-DAD. In accordance with the Ministry of Food and Drug Safety's health functional food guidelines, the analysis method was verified for specificity, accuracy, precision, limit of quantification, and linearity. The analysis method established in this study showed more than 0.9989 of the correlation coefficient values (r²) for the calibration. The total recovery rates of isoquercitrin and hyperoside were 100.55 and 98.87% with 0.14-0.78 and 0.47-0.67% of the relative standard deviation, respectively. Therefore, it was suggested that the new analytical method would be applied to standardize raw materials and high value-added products originated from the leaves of the S. koreana in the future.

• 한국수산과학회지
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• 제49권4호
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• pp.445-453
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• 2016

Seahorse Hippocampus abdominalis a marine teleost fish, has long been used as one of the essential materials in traditional Chinese medicine. However, the uses of seahorse have been limited due to its high cost, despite its beneficial biological activities. Seahorse has not been widely explored for its biofunctional properties and active components. In the present study, the enzymatic hydrolysates of seahorse were prepared by using two digestive enzymes (trypsin and pepsin) and five food grade enzymes (neutrase, protamex, alcalase, kojizyme, and flavourzyme). The enzymatic hydrolysates indicated higher hydrolysis yields than its water extract. Among them, the distilled water-pepsin hydrolysate (DP) which was obtained by distilled water extraction followed by pepsin hydrolysis, showed the highest yield and protein content as well as the highest alkyl radical scavenging activity. Also, it provided protective effects against oxidative stress induced by AAPH in vero cell and zebrafish. Further fractionation based on the molecular weight was carried out to identify it’s active components, and < 5 kDa (less than 5 kDa) molecular weight fraction was confirmed to have the highest antioxidant activity. In conclusion, this study suggests that DP of seahorse has antioxidant properties, and might be a novel and useful material from the marine origin for healthy functional foods and cosmetics.

• Journal of Forest and Environmental Science
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• 제26권1호
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• pp.37-51
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• 2010

A mushroom as a functional food material is being increasingly used in these days. It has been that about 1,500 species of mushrooms grow naturally in Asia. Among them about 700 species are edible, but about 30-50 species are poisonous. Even a poisonous mushroom is a very interesting subject for the dietary life research from biochemical viewpoint due to the fact that any mushroom can be the physiological active material. Not a few identified poisonous materials are being used as research reagents thus far. It is suggested that more useful materials could be obtained by progresses in future researches. The identification of the poisonous ingredient can open up a route for mushroom intoxication cure. It is known that cell destruction and troubles on skin, liver, and kidney troubles can be occurred from mushroom intoxication. Recently the author reported as a collection of treatises regarding anti-tumor substances of mushroom (2005), antibiotic substances of mushroom (2006), living-body functional and control substance (2007), and medicinal mushroom commonly used in Korean herbal medicines (2008). In this sequel, published research data on protoplasm, nerve, stomach and intestines, malodor, and acute pain poison of mushroom are collected and reported.

• 생명과학회지
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• 제25권9호
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• pp.1072-1079
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• 2015

발효 폐기물의 대부분을 차지하는 주박은 발효 후 알코올성 발효액을 필터, 원심분리 또는 증류 후에 얻어지는 찌꺼기 및 1차 제조된 제품의 숙성과정 중에 생성되는 찌꺼기를 말한다. 국내 전통주 주박은 식용 원재료와 다양한 약용작물을 첨가하고 이를 누룩으로 발효시켜 제조하여, 안전성이 확보되면서 생리활성이 우수한 장점을 가지고 있다. 최근의 탁주 위주의 전통주 시장 성장으로 주박 생산량은 빠르게 증가되고 있으며, 이의 폐기물 처리는 더욱 엄격해지고 있으나, 국내에서는 아직 전통주 주박의 효율적인 재이용에 대한 연구는 제한적이다. 본 총설에서는 전통주 주박의 산업동향, 연구동향 및 특허동향을 분석하고, 전통주 주박의 유용생리활성물질들을 제시하였다. 전통주 주박은 단순 발효폐기물이라는 인식에서 벗어나, 식량, 식품첨가물, 비료, 사료, 미생물 배양원, 기능성 식품소재, 향장 및 미용소재의 고부가가치 발효소재 공급원이라는 인식의 전환이 필요하며, 향후 재처리 기술, 유용물질 회수기술 개발을 위한 체계적인 산학관 연계와 연구가 필요하다.