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Preparation of Functional Cosmetics Containing $\beta$-carotene Derived from Recombinant Escherichia coli and Evaluation of Anti-wrinkle Efficacy by Clinical Testing  

Kim, You-Geun (KB Cosmetics)
Lee, Young-Hoon (KB Cosmetics)
Kang, Moon-Kook (Chungdam White Clinic)
Lee, Byung-Hak (Whiteview Clinic)
Yun, Jun-Ki (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University)
Kim, Sung-Bae (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University)
Kim, Chang-Joon (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University)
Publication Information
Microbiology and Biotechnology Letters / v.37, no.4, 2009 , pp. 399-404 More about this Journal
Abstract
This paper described the formulation of functional cosmetics and evaluation of anti-wrinkle efficacy in clinical test. Cosmetics were formulated with highly purified $\beta$-carotene obtained from the culture broth of recombinant E.coli cells. Edible oil for solubilizing $\beta$-carotene, vitamine E for long-term storage, detergent/stabilizer (2.0%) for the complete formation of oil/water emulsion, dimethicone (0.35%) for good skin care, and sorbitol for skin moisturizer were also added as ingredients. Physical or chemical degradation of formulated products stored at $0^{\circ}C$, $25^{\circ}C$, or $40^{\circ}C$ was not observed for 60-day testing period. In clinical test, 68% of applicants observed wrinkle decrease after 8-week treatment. This result indicates that newly formulated cosmetics have strong potential for improving wrinkle skin care.
Keywords
Recombinant Escherichia coli; $\beta$-carotene; functional cosmetics; formulation; clinical test;
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