• Molecules and Cells
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• v.38 no.4
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• pp.318-326
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• 2015

We previously reported that the SbROMT3syn recombinant protein catalyzes the production of the methylated resveratrol derivatives pinostilbene and pterostilbene by methylating substrate resveratrol in recombinant E. coli. To further study the production of stilbene compounds in E. coli by the expression of enzymes involved in stilbene biosynthesis, we isolated three stilbene synthase (STS) genes from rhubarb, peanut, and grape as well as two resveratrol O-methyltransferase (ROMT) genes from grape and sorghum. The ability of RpSTS to produce resveratrol in recombinant E. coli was compared with other AhSTS and VrSTS genes. Out of three STS, only AhSTS was able to produce resveratrol from p-coumaric acid. Thus, to improve the solubility of RpSTS, VrROMT, and SbROMT3 in E. coli, we synthesized the RpSTS, VrROMT and SbROMT3 genes following codon-optimization and expressed one or both genes together with the cinnamate/4-coumarate:coenzyme A ligase (CCL) gene from Streptomyces coelicolor. Our HPLC and LC-MS analyses showed that recombinant E. coli expressing both ScCCL and RpSTSsyn led to the production of resveratrol when p-coumaric acid was used as the precursor. In addition, incorporation of SbROMT3syn in recombinant E. coli cells produced resveratrol and its mono-methylated derivative, pinostilbene, as the major products from p-coumaric acid. However, very small amounts of pterostilbene were only detectable in the recombinant E. coli cells expressing the ScCCL, RpSTSsyn and SbROMT3syn genes. These results suggest that RpSTSsyn exhibits an enhanced enzyme activity to produce resveratrol and SbROMT3syn catalyzes the methylation of resveratrol to produce pinostilbene in E. coli cells.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2022.11a
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• pp.45-46
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• 2022

Recently, it is necessary to establish an eco-friendly construction system through recycling of waste material management at construction sites. This is required to reflect the times that require a carbon reduction system in construction as part of domestic and overseas carbon dioxide reduction. Therefore, we intend to establish eco-friendly construction management as part of resource recycling and carbon reduction through recycling technology for waste fibers generated at construction sites.

• The Research Journal of the Costume Culture
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• v.19 no.1
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• pp.119-127
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• 2011

This purpose of this study was to analyze the formation and look into design characteristics by types and method of expression on multifunctional eco-friendly fashion design. Total 191 pieces of multifunctional fashion design photographs were collected through fashion collection from 2000 S/S to S/S 2010 F/W on the website. First, the characteristics of multifunctional eco-friendly fashion design used squared silhouette, achromatic colors, plain patterns for pollution control, hard materials to prolong the product, it was clear that details were minimized to save resources. Second, there were 5 changeable types of multifunctional eco-friendly fashion design which were changes in changing forms, material changes, item changes, detail changes and complex changes. Third, as the result of changeable types by method of expression, the changing forms were expressed by removable, material changes by reversible, detail changes by open and close and item changes by shifting. Forth, the formative properties of multifunctional eco-friendly fashion design had flexibility, multifunction, versatility and amusing. Therefore, this study will be helpful in planning multifunctional eco-friendly fashion design according to the kind of formative characteristics, changeable types, method of expression and provide concrete fundamental materials for the expert in clothing on the base of objective data through statistical analysis.

• Journal of the Society of Disaster Information
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• v.19 no.4
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• pp.747-756
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• 2023

Purpose: In this study, a grout material mixed using non-alkaline silica-based materials, which is an eco-friendly injection material to stabilize ground, is investigated to improve conventional problems. Method: The homogel specimens of Eco-Friendly Non-Alkaline Silica Sol (ENASS) and L.W. and S.G.R., representative silicate grouting are manufactured. Physicochemical and engineering properties of the specimens are evaluated in laboratory with uniaxial compression strength, hydraulic conductivity, shrinkage, chemical resistance, elution, fish poison, waste leaching. Result: Laboratory test results show that the ENASS was superior in all aspects compared to the existing injection matirial. The suitability of the grout material with ENASS is investigated with filed tests. Conclusion: The results of laboratory and field tests demonstrates that the grout material with ENASS is eco-friendly material that increases the strength, decreases the permeability, and discharges pollutants without leaching.

• Journal of Microbiology and Biotechnology
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• v.27 no.6
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• pp.1112-1119
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• 2017

The aim of this study was to develop a potential process for bioethanol production from Hydrodictyon reticulatum (HR), a filamentous freshwater alga, using Saccharomyces cerevisiae (KCTC7017). From the sugar solutions prepared by the four different hydrolysis methods, bioethanol production ranged from 11.0 g/100 g dried material (acid hydrolysis) to 22.3 g/100 g dried material (enzymatic hydrolysis, EH). Bioethanol was fermented from a highly concentrated sugar solution obtained by a decompression-mediated (vacuum) enrichment method (VE). As the results, ethanol was more efficiently produced from HR when sugar solutions were concentrated by VE following EH (EH/VE). Using multiple feeding of the sugar solution prepared by EH/VE from HR, ethanol reached up to a concentration of 54.3 g/l, corresponding to 24.9 g/100 g dried material, which attained the economic level of product concentration (approximately 5%). The results indicate that by using HR, it is feasible to establish a bioethanol production process, which is effective for using microalgae as the raw material for ethanol production.

• Korean Institute of Interior Design Journal
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• v.21 no.1
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• pp.220-227
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• 2012

After The Ramsar Convention took place in Korea in 2008, public interest in environment heightened, and the government has been allocating its budget for conserving our environment. Therefore, the present study focuses on eco-friendly spatial images particularly shown in ecological museums in Korea which recognizes the value of the environment and the ecology and tries to both protect them and alert people about it. The purpose of this study is proving what consist of eco-friendly spatial images by analyzing the expressive word of eco-friendly images and the image of space, providing a basic data for future space planning of ecological museums. To do this, the present study proceeds in three steps. First of all, the base of research in analyzing stage is firmly established by grasping general theories and terms regarding spatial image. As a second step, the composition and the characteristics of exhibition is clarified through on-spot investigation to provide comparative data for spatial image assessment in the future. Also through this step, we could understand how the exhibits are designed currently. In the last stage of research, expressive words regarding eco-friendly spatial images are extracted and used to analyze the spatial image of ecological museums. And the following three conclusions is deduced. First, the expressive words of eco-friendly spatial image that are extracted are as following: "healthy", "coexisting", "clean", "blending", "warm", "soft", "lively", "pure", "cool", "fresh ", "comfortable", "relaxed", "mild", "free", "harmonious", and "healing". As the second conclusion, color, and material, the formation which is an architectural factor did not have a great impact on forming eco-friendly image, but the color and the material did. The third conclusion was that the display with natural aspects actively utilized increased eco-friendly spatial image.

• The Journal of the Korea Contents Association
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• v.11 no.10
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• pp.209-216
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• 2011

This paper, the shielding material is suggested using by the korean paper, lacquer, and charcoal those can be used more eco-friendly materials for the interior design. Also, it is designed the image-wall for improving of the electromagnetic shielding and the beauty. Suggested shielding material is manufactured with the thickness of 2 mm by the printing method of the lacquer and charcoal on the korean paper. This material has the shielding effectiveness over 95% until 2 GHz. The application for the interior design using more eco-friendly material can be reduced the effect of electromagnetic wave and the chemical substances like as the formaldehyde and the volatile organic compound (VOC).

• Proceedings of the Korean Institute of Building Construction Conference
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• 2020.06a
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• pp.148-149
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• 2020

Currently, the most commonly used EPS insulation material has been mainly used because its ease of adhesion with concrete. However, due to poor adhesion with wallpaper, separate adhesion needs to be strengthened and there are cases of breakage or grooves in the process of dismantling the mold. The biggest problem is that when a fire breaks out, various harmful substances are present and highly flammable. Cork used in this study is a truly eco-friendly building material that is taken from between the outer and inner bark of cork trees and does not damage the wood. Also, it is a porous material that is made up of countless cells and contains an air gap between the cells. It is very light in weight between 0.06 and 0.07 and has excellent insulation with a heat conductivity of 0.04W/mK. In addition, it has high stability in the topic of conversation because it does not produce harmful gas when burned and has self-sustaining properties. However, research on cork, an eco-friendly building material with excellent performance to date, is scarce Therefore, we encourage existing scholars to raise interest in new eco-friendly building materials through this study. It also aims to manufacture insulation boards with new inorganic properties using the low weight and heat conductivity held by the cork.

• Korean Institute of Interior Design Journal
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• v.21 no.1
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• pp.86-94
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• 2012

The purpose of this study was to examine the importance of eco-friendly design elements related to residential space by residents and what element they prefer. To achieve this, 18 eco-friendly design elements related to residential space were selected from the Green Building Certification Criteria and the relevant research papers. These elements were categorized into four areas; the ecological environment area (4 elements), the indoor environmental climate one (4 elements), the energy and resource saving one (6 ones), and the material one(4 ones). On the base of these 18 elements, the importance degree of each element was examined by using 5-point Likert scale. The subjects were 299 housewives living in apartment houses in Jangyu new town, Gimhae. The subjects perceived strongly the importance of eco-friendly design elements, but many of them did not know about Green Building Certification Criteria. Overall importance of 18 elements by the residents was very high. Also, the indoor environmental climate area was considered as the most important one, followed by the energy and resource saving area, the material area, and the ecological environment one.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2005.10a
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• pp.209-210
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• 2005

This research aims at proposing new space design model with eco-friendly material, paper, for sustainable life mode. As pro-natural paper which is feasible for secondary processing in interior design, it's significance lies in its easy adaptability to nature and depreciation of environment pollution. The target space which needs alterability is designed with corrugated paper as its eco-friendly and changeable features as corrugated paper made out of wood pulp would return to nature after decomposition. If the material will be recycling, reuseable, and renewable which are eco-friendly and durable, then sustainable space design would be created.