• 대한한의학회지
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• 제26권3호
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• pp.1-12
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• 2005

Objectives : Substantial evidence suggests that reinforcing effects of nicotine can be mediated by the mesolimbic dopaminergic system. It has been shown that repeated injections of nicotine produce an increase in locomotor activity and expression of the immediate-early gene, c-fos, in the dopaminergic target areas. Herbal medicine as a therapeutic intervention has been widely used for the treatment of mental dysfunction. Many studies have shown that Radix Scutellariae (RS) can affect the biochemical balance in the central nervous system. Tn order to investigate whether RS has an influence on nicotine-induced behavioral sensitization, we examined the effect of RS on nicotine-induced locomotor activity and c-fos expression in the striatum and nucleus accumbens utilizing the fos-tike immunohistochemistry (FLI). Methods : Male SD rats received RS (200mg/kg, i.p.) 30min before repeated daily injections of nicotine (0.4mg/kg, s.c.) for 7 days. This was followed by withdrawal for 3 days and one challenge for 1 day. Results : System challenge with nicotine produced a much larger increase in locomotor activity and accumbal FLI. Pretreatment with RS significantly inhibited nicotine-induced locomotor activity and FLI in the striatum and nucleus accumbens. Conclusions : These results demonstrate that reduction in locomotor activity by RS may be reflected by reduction of dopamine release and postsynaptic neuronal activity in the striatum and nucleus accumbens. Our results suggest that RS may have therapeutic effect on nicotine addiction.

• Journal of Animal Science and Technology
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• 제47권4호
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• pp.555-564
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• 2005

본 연구는 비육돈에 대한 황토의 주성분인 zeolite의 첨가효과를 구명하기 위하여 증체량, 사료요구율, 육질, 악취제거 및 경제성 등을 조사하였으며 생후 93일령의 비육돈 120두(4처리$\time$3반복$\time$10두)를 공시하여 출하시까지 70일간 사양시험을 실시하였다. 일당증체량은 비육돈 전기의 경우 zeolite 4% 첨가구가 1.00kg으로서 대조구의 0.8kg에 비하여 약 25%로 크게 향상되었다(P<0.05). 그러나 비육돈 후기의 일당 증체량은 대조구(0.78kg)를 포함한 모든 처리구에서 0.74-0.83kg으로서 유의한 차이는 없었다. 사료요구율은 시험 전기간 동안 대조구에서는 3.43으로서 가장 높았으며, 그 다음은 zeolite 1%구와 2%로서 각각 3.24와 3.13이었으며 zeolite 3% 첨가구는 3.00으로서 약 7.4%의 개선효과가 나타났다(P<0.05). 시험 전기간 동안의 $NH_3$ 가스 발생량은 zeolite 4% 첨가구가 대조구에 비하여 약 33.9-53.9%의 개선효과가 나타났다(P<0.05). 도체중과 등지방 두께는 대조구와 zeolite 첨가구 간에 차이가 없었으며, A등급 출현율은 zeolite 4% 첨가구가 60.00%로서 대조구의 36.67%에 비하여 높게 나타났다. 경제성 분석 결과, 증체 kg당 사료비는 zeolite 4% 첨가구는 910원, 2% 첨가구는 919원, 1% 첨가구는 941원 및 대조구는 982원으로서 zeolite를 4% 첨가함으로써 개선효과가 크게 나타났다. 이상의 결과, 비육돈에 대하여 zeolite를 4% 수준으로 첨가․급여하면 사료요구율, 육질 A등급 출현율, 분내 암모니아가스 발생량 및 경제성면에서 효과적인 것으로 사료된다.

• 만화애니메이션 연구
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• 통권45호
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• pp.367-396
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• 2016

나는 태국 방콕에 소재한 Mahidol 대학교 국제학부에서 애니메이션 교육자로서 지난 8년동안 태국의 신 세대 애니메이터의 작은 집단을 교육하는 특권을 지닌 위치에 있었다. 태국은 태국 고유의 애니메이션 실험적용이 그 동안 거의 없었으며, 그런 다양성에 접근도 인터넷 이전 시대에는 제한이 있었다. 이런 이유로 나의 연구 배경이 되는 특히 영국과 유럽의 풍요로운 애니메이션 역사를 강의실로 가져와 소개하려는 시도를 해왔다. 특히, 내가 가르치는 데 있어서 둔 목적은 초기의 (또한 현재의) 실험적/독립 애니메이터들이 탐구했던 매체, 양식과 텍스트 네러티브(서사) 선택에 관한 다양한 가능성을 소개하는 것이었다. 최초의 장편 상업 영화 "Khun Kluay"가 2006년에 출시된 이래로, 태국의 애니메이션 산업은 그간 외국에서 훈련 받고 일하고 돌아온 숙련된 인력을 형성하면서 상업적인 부면에서 여러 기복을 겪어왔다. 현재는 독립 애니메이션이 출현하기 시작하는 확실한 증거가 보인다. 태국 애니메이션에 관한 나의 연구의 연장으로, 본 논문은 선정된 독립작품에 대해서 그 주제를 비롯해 사용된 양식과 테크놀로지를 탐구하면서 세밀하게 분석하고자 한다. 그러나 동시에 해석에 대한 오류의 가능성도 인정할 수 밖에 없을 것이다. 나의 문화적 언어와 해석은 아웃사이더의 관점이기 때문에 본래 크리에이터의 경험을 초월할 수도 있고 대안적인 해석을 추가할 수도 있을 것이다. 본 논문은 작품의 내용과 아이디어와 문화적인 관점을 맥락화하고, 더 많은 태국의 작품들이 애니메이션 연구 학계에 시선을 모을 수 있는 것을 목적으로 한다.

• 수산해양교육연구
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• 제26권1호
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• pp.34-48
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• 2014

Marine algal assemblages on the artificial reefs at three stations (Haengwon, Geumneung, Pyoseon in Jeju-do) and the growth pattern of Ecklonia cava with water depth were studied before and after rocky cleaning. Nine algal species occurred at three artificial reefs before rocky cleaning in July 2012, however, 19 algal species were found at three artificial reefs after rocky cleaning in July 2013. In particular, 13 of 19 species in 2013 were replaced by different species which were not found in July 2012. Algal biomass rapidly increased in July 2013 after rocky cleaning. The nMDS plot based on the presence and absence data of macro-algal assemblages on the artificial reefs showed that the species compositions between artificial reefs were similar to each other before rocky cleaning operations in July of 2012. However, after rocky cleaning, the species composition of macro-algal assemblages in Haengwon region was similar to that in Pyoseon region while that in Geumneung region was different to those in Haengwon and Pyseon regions in July 2013. It is needed to clarify the suitable water depth for transplantation of perennial Phaeophyta E. cava. According to the data on seasonal changes in total length, total weight, blade length, blade width, blade weight of E. cava, it began to grow rapidly from April and reached to the maximum value in June, and then degeneration of the blade occurred in July and continued to early August. Although the total length of E. cava at 10m water depth was larger than those at 5 m and 15 m water depths, there was no significant difference statistically among water depths by ANOVA test. However, in the values of total weight, blade length, blade width, and blade weight, the growth patterns at 5 m water depth were larger than those at 10 m and 15 m. Thus, it is assumed that the most suitable water depth for transplantation and its maximum growth and effective release of zoospores of E. cava will be 7~10 m water depth.

• Journal of Plant Biotechnology
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• 제38권2호
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• pp.125-129
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• 2011

GM작물의 상용화를 위하여서는 반드시 환경위해성평가를 거쳐야 한다. 불행하게도 초기 event가 개발되고 기본적인 검토가 완료된 GM작물에 대하여 환경위해성평가를 수행하기 위하여서는 약 7백만에서 1,500만불의 경비가 소요되며 기간 또한 수년이 필요하다. 따라서 저자는 사용화를 위한 GM작물의 개발을 위한 프로젝트에 관여하는 개발자는 실험실 또는 온실단계의 아주 초기단계에서부터 환경위해성평가기준을 충분히 숙지하여야 한다고 제안한다. 기존의 많은 리뷰논몬에서 이미 지적된 것 과 같이 GM작물의 상용화과제에서 가장 큰 걸림돌(죽음의 계곡)은 초기 사상 (event)의 선발과정이며, 대부분이 이 단계에서 충분한 자료를 제시할 수 없어서 포기한다는 사실을 간과하여서는 안 될 것이다. 특히 지극히 기본적인 분자생물학 특성에 관한 자료들로서 예를 들어 도입유전자의 copy 수, 숙주의 게놈내 도입위치, 도입유전자의 주변 염기서열, 유전자 재배열과 도입유전자로 인한 숙주 내 기존유전자의 knock out 현상의 여부 등에 관한 충분한 자료를 제시하지 못하는 경우가 많으며 이는 GM 작물의 개발 초기단계에서부터 철저하게 검증이 이루어져야 하는 항목들이다. 국내의 경우, 농촌진흥청은 2011년부터 2020년까지 10년간 "차세대바이오그린 21 사업"을 발족하여 추진 중에 있으며 특히 국내용뿐만 아니라 중국의 사막화 지역 등 불량환경에 재배가 가능한 GM작물 등 해외로 수출을 위한 global GM작물의 개발을 위한 전략을 수립하여 추진 중에 있다. 따라서 저자는 환경위해성평가기준에 부합되는 초기 event의 선발 과정에 역점을 두어 사전에 분자생물학적 특성에 관한 RA기준을 고려하여 자체평가시스템을 도입하도록 제안 한다.

• Archives of Pharmacal Research
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• 제22권5호
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• pp.437-447
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• 1999

Type I diabetes, also known as insulin-dependent diabetes mellitus (IDDM) results from the destruction of insulin-producing pancreatic $\beta$ cells by a progressive $\beta$ cell-specific autoimmune process. The pathogenesis of autoimmune IDDM has been extensively studied for the past two decades using animal models such as the non-obese diabetic (NOD) mouse and the Bio-Breeding (BB) rat. However, the initial events that trigger the immune responses leading to the selective destruction of the $\beta$ cells are poorly understood. It is thought that $\beta$ cell auto-antigens are involved in the triggering of $\beta$ cell-specific autoimmunity. Among a dozen putative $\beta$ cell autoantigens, glutamic acid decarboxylase (GAD) has bee proposed as perhaps the strongest candidate in both humans and the NOD mouse. In the NOD mouse, GAD, as compared with other $\beta$ cell autoantigens, provokes the earliest T cell proliferative response. The suppression of GAD expression in the $\beta$ cells results in the prevention of autoimmune diabetes in NOD mice. In addition, the major populations of cells infiltrating the iselts during the early stage of insulitis in BB rats and NOD mice are macrophages and dendritic cells. The inactivation of macrophages in NOD mice results in the prevention of T cell mediated autoimmune diabetes. Macrophages are primary contributors to the creation of the immune environment conducive to the development and activation of $\beta$cell-specific Th1-type CD4+ T cells and CD8+ cytotoxic T cells that cause autoimmune diabetes in NOD mice. CD4+ and CD8+ T cells are both believed to be important for the destruction of $\beta$ cells. These cells, as final effectors, can kill the insulin-producing $\beta$ cells by the induction of apoptosis. In addition, CD8+ cytotoxic T cells release granzyme and cytolysin (perforin), which are also toxic to $\beta$ cells. In this way, macrophages, CD4+ T cells and CD8+ T cells act synergistically to kill the $\beta$ cells in conjunction with $\beta$ cell autoantigens and MHC class I and II antigens, resulting in the onset of autoimmune type I diabetes.

• Journal of Dairy Science and Biotechnology
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• 제34권1호
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• pp.37-41
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• 2016

본 연구에서는 착유시간에 따른 우유 및 환원유 내 멜라토닌 및 코티솔 농도 변화를 살펴보았고, 이를 바탕으로 긴장완화 및 수면의 질 개선 측면에서의 우유의 기능성 식품으로써의 개발 가능성을 탐색하였다. 연구 결과, 명기에 비해 일출전 암기에 착유한 우유에서 멜라토닌 농도가 유의적으로 높았다. 환원유 내 멜라토닌 농도 또한 우유와 동일한 일주기 리듬을 보였으나, 전반적으로 우유에 비해 멜라토닌 농도가 높은 것을 확인할 수 있었다. 이에 반해 빛 이외에도 운동, 스트레스 등과 같은 요인에 의해 영향을 받는 코티솔은 착유시간에 따른 유의적 차이를 보이지 않았다. 하지만 개체간 변이가 크지 않다고 가정하였을 때, 동지에 비해 하지에 착유한 우유 및 환원유 내 코티솔 농도가 유의적으로 높았다. 이러한 연구 결과를 바탕으로 할 때, 집유 시간을 조정하여 생산한 야간 착유 우유는 내인성 멜라토닌이 풍부한 식품이며, 수면유도에 도움을 줄 수 있는 기능성 식품으로써의 개발 가능성을 가지고 있는 것으로 사료된다. 하지만 본 연구는 착유시간에 따른 원유 및 환원유 내 멜라토닌 농도의 차이만을 보여줬다는 점에서 한계를 가진다. 그러므로 착유시간에 따른 이러한 차이가 실질적으로 우유를 섭취하였을 때, 체내의 수면유도 생리활성에 영향을 미치는지 추가적으로 살펴볼 필요가 있다. 또한, 계절에 따른 우유 내 각 호르몬의 변화를 정확히 파악하기 위하여 동일한 개체를 대상으로 한 추가 연구가 필요하다.

• The Korean Journal of Physiology and Pharmacology
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• 제3권1호
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• pp.101-117
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• 1999

Amyotrophic lateral sclerosis (ALS) is a degenerative neuromuscular disease of unknown etiology in which the upper and lower motor neurons are progressively destroyed. Recent evidences support the role of autoimmune mechanisms in the pathogenesis of ALS. This study investigated the effects of sera from ALS patients on neuromuscular transmission in phrenic nerve-hemidiaphragm preparations and on calcium currents of single isolated dorsal root ganglion (DRG) cells in mice. Mice were injected with either control sera from healthy adults or ALS sera from 18 patients with ALS of sporadic form, for three days. Miniature end plate potential (MEPP) and nerve-evoked end plate potential (EPP) were measured using intracellular recording technique and the quantal content was determined. Single isolated DRG cells were voltage-clamped with the whole-cell configuration and membrane currents were recorded. Sera from 14 of 18 ALS patients caused a significant increase in MEPP frequency in normal Ringer's solution $(4.62{\pm}0.14\;Hz)$ compared with the control $(2.18{\pm}0.15\;Hz).$ In a high $Mg^{2+}/low\;Ca^{2+}$ solution, sera from 13 of 18 ALS patients caused a significant increase in MEPP frequency, from $2.18{\pm}0.31$ Hz to $6.09{\pm}0.38$ Hz. Sera from 11 of 18 patients produced a significant increase of nerve-evoked EPP amplitude, from $0.92{\pm}0.05$ mV to $1.30{\pm}0.04$ mV, while the other seven ALS sera did not alter EPP amplitude. In the ALS group, EPP quantal content was also elevated by the sera of 14 patients (from $1.49{\pm}0.07$ to $2.35{\pm}0.07).$ MEPP frequency and amplitude in wobbler mouse were $4.03{\pm}0.53$ Hz and $1.37{\pm}0.18$ mV, respectively, which were significantly higher than those of wobbler controls (wobblers without the symptoms of wobbler). Sera from ALS patients significantly reduced HVA calcium currents of DRG cells to 42.7% at -10 mV. Furthermore, the inactivation curve shifted to more negative potentials with its half-inactivation potential changed by 6.98 mV. There were, however, significant changes neither in the reversal potential of $I_{Ca}$ nor in the I-V curve. From these results it was concluded that: 1) The serum factors of sporadic ALS patients increase neuromuscular transmission and can alter motor nerve terminal presynaptic function. This suggests that ALS serum factors may play an important role in the early stage of ALS, and 2) Calcium currents in DRG cells were reduced and rapidly inactivated by ALS sera, suggesting that in these cells, ALS serum factors may exert interaction with the calcium channel.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2008년도 추계 학술발표회
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• pp.1115-1123
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• 2008

When constructing projects such as road embankments, bridge approaches, dikes or buildings on soft, compressible soils, significant settlements may occur due to the consolidation of these soils under the superimposed loads. The compressibility of the soil skeleton of a soft clay is influenced by such factors as structure and fabric, stress path, temperature and loading rate. Although it is possible to determine appropriate relations and the corresponding material parameters in the laboratory, it is well known that sample disturbance due to stress release, temperature change and moisture content change can have a profound effect on the compressibility of a clay. The early research of Tezaghi and Casagrande has had a lasting influence on our interpretation of consolidation data. The 24 hour, incremental load, oedometer test has become, more or less, the standard procedure for determining the one-dimensional, stress-strain behavior of clays. An important notion relates to the interpretation of the data is the ore-consolidation pressure ${\sigma}_p$, which is located approximately at the break in the slope on the curve. From a practical point of view, this pressure is usually viewed as corresponding to the maximum past effective stress supported by the soil. Researchers have shown, however, that the value of ${\sigma}_p$ depends on the test procedure. furthermore, owing to sampling disturbance, the results of the laboratory consolidation test must be corrected to better capture the in-situ compressibility characteristics. The corrections apply, strictly speaking, to soils where the relation between strain and effective stress is time independent. An important assumption in Terzaghi's one-dimensional theory of consolidation is that the soil skeleton behaves elastically. On the other hand, Buisman recognized that creep deformations in settlement analysis can be important. this has led to extensions to Terzaghi's theory by various investigators, including the applicant and coworkers. The main object of this study is to suggestion the modified compression index value to predict settlements by back calculating the $C_c$ from different numerical models, which are giving best prediction settlements for multi layers including very thick soft clay.

• 한국중재학회지:중재연구
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• 제25권4호
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• pp.3-23
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• 2015

The Chevron saga including Chevron/TexPet v. Ecuador, PCA Case No. 34877(hereinafter referred to as "Chevron I") and Chevron/TexPet v. Ecuador, PCA Case No. 2009-23(hereinafter referred to as "Chevron II") started out of domestic litigations between TexPet and Ecuador in the early 1990s. In Chevron I, the Tribunal decided that Article 2(7) of the U.S.-Ecuador BIT on effective means of provision was breached because of undue delays in the seven legal proceedings TexPet had brought against Ecuador in respect to contractual obligations. In Chevron II, it was contended that through the actions and inactions of the judiciary and the executive, Ecuador breached her several obligations under the BIT. Ecuador objected to the jurisdiction of the Tribunal because TexPet's investment was terminated in 1992, and because Chevron is not a party to the 1995 Settlement Agreement and 1998 Final Release. In its Interim Award on Jurisdiction and Admissibility, the Tribunal applied a prima facie standard to the facts alleged by the Claimants but denied by the Respondent, and decided that questions in respect of the Respondent's jurisdictional objections should be joined to the merits under Article 21(4) of the UNCITRAL Arbitration Rules. In the merits phase of Chevron II, the Tribunal divided the merits of the Parties' dispute into two parts, entitled "Track 1" and "Track 2". In its Partial Award on Track 1, the Tribunal decided that Chevron is a "Releasee" under the 1995 Settlement Agreement. In a decision on "Track 1B", the Tribunal decided that the Lago Agrio complaint cannot be read as pleading "exclusively" or "only" diffuse claims, and that, to this extent, the Claimants' reliance on the 1995 Settlement Agreement as a complete bar to the Lago Agrio complaint must fail, as a matter of Ecuadorian law. The Tribunal maintained the position that the Parties' disputes on both merit and jurisdiction should be reserved for Track 2. It remains to be seen how the Tribunal addresses the Claimants' allegations of multiple denials of justice under international law against the judgments of the Respondent's Courts, together with the Respondent's jurisdictional objections in Track 2 of the arbitration.