• Title/Summary/Keyword: early development

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Expression of Immune-Related Genes during Loach (Misgurnus anguillicaudatus) Embryonic and Early Larval Development

  • Lee, Jang Wook;Kim, Jung Eun;Goo, In Bon;Hwang, Ju-Ae;Im, Jea Hyun;Choi, Hye-Sung;Lee, Jeong-Ho
    • Development and Reproduction
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    • v.19 no.4
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    • pp.181-187
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    • 2015
  • Early life stage mortality in fish is one of the problems faced by loach aquaculture. However, our understanding of immune system in early life stage fish is still incomplete, and the information available is restricted to a few fish species. In the present work, we investigated the expression of immune-related transcripts in loach during early development. In fishes, recombination-activating gene 1 (RAG-1) and sacsin (SACS) have been considered as immunological function. In this study, the expression of the both genes was assessed throughout the early developmental stages of loach using real-time PCR method. maRAG-1 mRNA was first detected in 0 dph, observed the increased mostly until 40 dph. Significant expression of maRAG-1 was detected in 0 to 40 dph. These patterns of expression may suggest that the loach start to develop its function after hatching. On the other hand, maSACS was detected in unfertilized oocyte to molura stages and 0 to 40 dph. maSACS mRNA transcripts were detected in unfertilized oocytes, suggesting that they are maternally transferred.

Properties of Strength Development Under Various Curing Condition at Early Age of Cement Mortar Using Agent for Enduring Cold Weather (내한성 혼화제를 이용한 시멘트 모르타르의 초기양생 온도변화에 따른 강도증진 특성)

  • Han, Cheon-Goo;Hong, Sang-Hee;Kim, Hyun-Woo
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.5 no.2
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    • pp.191-197
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    • 2001
  • In this study, the admixtures for agents for enduring cold weather used widely are collected and applied to cement mortar to analyze the strength development due to variation of curing temperature at early age. The test results show that anti-freezing admixture have some problems due to high chloride content, which may cause the corrosion of reinforcement embedded in concrete. However, the mortar applied by accelerator and another kind of agent for enduring cold weather produced by S company lead to delay of strength development in low temperature. Also, it is clarified that there are no significant problems for cement mortar in strength development due to low temperature if a suitable kind of agent enduring cold weather is used and cement mortar is cured for more than $7.5^{\circ}D.D$ at early age.

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The Effects of pH on Early Embryo Development of Sea Urchins (수소이온농도(pH)가 성게의 초기 배 발생에 미치는 영양)

  • 유춘만
    • Journal of Environmental Health Sciences
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    • v.30 no.2
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    • pp.104-107
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    • 2004
  • This study was conducted to evaluate the effects of pH, using gametes, embryos and early development system of three sea urchins species, Hemicentrotus pulcherrimus, Anthocidaris crassispina and Scaphechinus brevis. As the result of performing effects of pH on early embryo development, the conditions of appropriate pH on formation of normal pluteus were pH 7.0-8.0 for H. pulcherrimus, A. crassispina and S. brevis. Otherwise, the conditions of pH 5.0, pH 6.0, pH 9.0 and pH 10.0 damaged the development of early embryos of each testing animal moderately or strongly.

The Practical use of early strength development technology for form stripping earlier than normal state in apartment (공동주택 거푸집 조기해체를 위한 조기강도 발현기술 실용화)

  • Kim, Gyu-Dong;Lee, Seung-Hoon;La, Woon;Hwang, Yin-Seong;Han, Cheon-Goo
    • Proceedings of the Korea Concrete Institute Conference
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    • 2004.11a
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    • pp.765-768
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    • 2004
  • This study reports the site application of the early strength development technology for form stripping earlier than normal state in the apartment construction. It is a method of framework rationalization and it needs the technology of early strength development concrete and 6-day cycle process. We applied the method to the apartment construction to reduce the floor construction period and to maximize the economical effect by reduction of the period of total construction. As a result, we confirmed the superior property of the early strength development of the concrete and reliable success of 6-day cycle process.

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Evaluation of Offshore Seawater Qualify using Gametes and Embryos of Starfishes (Asterina pectinifera) (불가사리 알을 이용한 연안해수의 수질 평가)

  • Yu Chun Man;Lee Jong Bin;Park Jong Cheon;Joo Hyun Soo
    • Korean Journal of Environmental Biology
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    • v.22 no.3
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    • pp.376-380
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    • 2004
  • In August 2003, the water quality of offshore waters along the Incheon coast of Korea was evaluated by biological evaluation using gametes, embryos and early development systems of a starfish species (Asterina pectinifera). As the result of performing biological evaluations on seawater samples from a total thirteen sites, the formation rate of normal larva was 16-68%. At seawater sample from site 5 and 13, formation rate of normal larva averaged 16%, the most abnormal rate hindering the early embryo development of the experimental animal, while that of site 3 averaged 68%, the highest formation rate of normal larva. At seawater sample from site 2, 4, 7, 9, 10, 11, 12, formation rate of normal larva averaged 33-54%, those which damage the development of early embryos slightly. At seawater sample from site 1, 5, 6, 8, 13, formation rate of normal larva averaged 16-28%, those which damage the development of early embryos strongly.

Domestic Production Process of Early Korean Broadcast CG Equipment

  • Nah, So-Mi
    • Journal of Multimedia Information System
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    • v.8 no.4
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    • pp.285-294
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    • 2021
  • The development process of domestic CG (Computer Graphics) needs to be studied by classifying the technical part and the design part. This paper analyzes how early domestic broadcast equipment evolved and focused on what went through the development process. Domestic broadcasting companies have introduced and used overseas equipment and have begun to develop their own technology. The development process of the early domestic production technology of broadcasting stations was classified into Character Generator, On-line Real-Time Graphic and Sport Coder. It was found that the orientation of broadcasting technology was conclusively focused on visualization, with socio-cultural factors acting along with the evolution of hardware and software. This research is meaningful in reorganizing the history of the development process of domestic CG technology in the early days through previous research (primary verification), newspaper articles and news coverage (secondary verification). This paper looks at what is missing by regaining the past of CG, and argues that with the advent of new technologies today, we must develop through appropriate division of roles and collaboration between engineers and designers.

Determination of the Protecting Periods of Frost Damage at Early Age in Cold Weather Concreting (한중콘크리트의 초기 동해 방지를 위한 초기 양생기간의 산정)

  • 한천구;한민철
    • Journal of the Korea Concrete Institute
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    • v.12 no.3
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    • pp.47-55
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    • 2000
  • Protections from the frost damage at early ages are one of the serious problems to be considered in cold weather concreting. Frost damage at early ages brings about the harmful influences on the concrete structures such as surface cracks and declination of strength development. Therefore, in this paper, protecting periods of frost damage at early ages according to the standard specifications provided in KCI(Korean Concrete Institute) are suggested by appling logistic curve, which evaluates the strength development of concrete with maturity. W/B, kinds of cement and curing temperatures are selected as test parameters. According to the results, the estimation of strength development by logistic curve has a good agreement between calculated values and measured values. As W/B and compressive strength for protecting from frost damages at early ages increase, it is prolonged. It shows that the protecting periods of FAC(Fly Ash Cement) and BSC(Blast-furnace Slag Cement) concrete are longer than those of OPC(Ordinary Portland Cement) concrete. The protecting peridos from frost damage at early age by JASS are somewhat shorter than those by this paper.

Micromechanics based Models for Pore-Sructure Formation and Hydration Heat in Early-Age Concrete (초기재령 콘크리트의 세공구조 형성 및 발영특성에 관한 미시역학적 모델)

  • 조호진;박상순;송하원;변근주
    • Proceedings of the Korea Concrete Institute Conference
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    • 1999.04a
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    • pp.123-128
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    • 1999
  • Recently, as a performance based design concept is introduced, assurance of expected performances on serviceability and safety in the whole span of life is exactly requested. So, quantitative assessments about durability related properties of concrete in early-age long term are come to necessary, Especially in early age, deterioration which affects long-term durability performance can be occurred by hydration heat and shrinkage, so development of reasonable hydration heat model which can simulate early age behavior is necessary. The micor-pore structure formation property also affects shrinkage behavior in early age and carbonations and chloride ion penetration characteristic in long term, So, for the quantitative assessment on durability performance of concrete, modelings of early age concrete based on hydration process and micor-pore structure formation characteristics are important. In this paper, a micromechanics based hydration heat evolution model is adopted and a quantitative model which can simulate micro-pore structure development is also verified with experimental results. The models can be used effectively to simulate the early-age behavior of concrete composed of different mix proportions.

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Effect of the Culture Media of Bovine Oviductal Epithelium on Development of the Early Bovine Embryos Derived from in vitro Fertilization (소 난관상피세포배양액이 체외수정 유래 분할란의 발육에 미치는 영향)

  • Park Jong-Im;Hwang Woo-Suk;Jo Choong-Ho;Lee Byeong-Chun
    • Journal of Veterinary Clinics
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    • v.9 no.1
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    • pp.323-332
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    • 1992
  • The present study was carried out to examine the effect of oviduct epithelium and its conditioned medium on e development of early bovine embryos in vitro. Oocytes obtained from ovarian follicles of slaughtered cows were cultured in TCM199 with 10% fetal calf serum for 22-24hrs and then fertillzed in vitro using frozen-thawed semen treated with BO-caffein, BO-BSA(20mM heparin added). Oviduct epithelium was collected in each stage of the estrus cycle and conditioned medium was the medium in which oviduct epithelium in early luteal stage was cultured. In vitro fertilized bovine embryos of 1~2 cell were co-cultured with oviduct epithelium from different estrus cycles, cultured in conditioned medium, and cultured in rabbit oviduct. The cleavage rates of in vitro fertilized early bovine embryos co-cultured with oviduct epithelial cell from early luteal, luteal and follicular phase of estrus cycle(67.2~70.8%) and cultured in conditioned medium(56.7%) were significantly(p<0.05) higher than that of the control(44.2%) The rate of development to morula or blastocyst stage in oviduct epithelial cell co-culture(15.3~32.5%) from three phase of estrus cycles and conditioned medium(14.5%) were significantly(p<0.05) higher than that of the control(5.2%). The oviduct epithelial cell from early luteal phase gave a significantly( p<0.05) higher rate of development to morula or blastocyst stage than both luteal and follicular phase. The results of in vivo culture in rabbit oviduct of early bovine embryos were 52.1% for the cleavage rate and 26.7% for the rate of development to morula or blastocyst stage.

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