• Journal of Plant Biotechnology
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• 제8권1호
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• pp.9-14
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• 2006

As an alternative method to produce low cost reagents for immunodiagnosis and protect the plants from viral disease, a gene encoding a single chain variable fragment(scFv) recombinant antibody targeted to the coat protein of cucumber mosaic virus (CMV) was expressed in Nacotiana tabacum. The source of the scFv recombinant antibody gene was from spleen tissue of an immunized mouse. The gene was initially cloned into the pCANTAB5E phagemid and expressed in E. coli. In the following study, the antibody gene was subcloned into the plant expression vector, pCAMBIA-1301 and introduced into tobacco leaf tissue via Agrobacterium tumefacients mediated transformation. After transformation, 56 out of 58 plants were shown to carry the desired anti-CMV scFv gene by PCR analysis. Overall, only 12.5% of the 56 putative transgenic plants were found to express the antibody to a detectable level.

• 정보처리학회논문지D
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• 제9D권4호
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• pp.667-676
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• 2002

인터넷으로 대표되는 디지털 환경은 생활 전반에 걸쳐 엄청난 변화를 주고 있으며 산업체의 비즈니스 방식과 업무 수행 방식을 빠른 속도로 바꾸어 놓고 있다. 전통 산업인 제조업체에도 인터넷과 웹 관련 기술, 초고속정보통신망을 활용하여 기존의 업무 프로세스를 개선하고 생산성 향상 및 관리 효율을 극대화하고자 하는 e-전환(e-Transformation)의 추진이 활발하게 전개되고 있다. 본 논문에서는 공장의 관리 효율 제고를 목적으로 국내외에 분산된 공장들의 현재 시점 가동 현황을 통합하여 언제 어디에서나 모니터링하기 위한 웹 기반 공장 모니터링시스템을 설계하고 구현하였다. 제안된 시스템은 웹 기반의 시스템 구조와 수행 기능, 그리고 관리 데이터를 표준화시키고, UML(Unified Modeling Language)을 이용하여 설계하였으며, ASP(Active Server Pages)를 활용하여 웹 기능을 구현하였다. 구현된 웹 기반 공장 모니터링 시스템은 K 주식회사 타이어사업부의 두개 공장(Kl, K2)을 대상으로 적용하고 있으며, 적용 결과는 전체 공장의 운영 상황을 종합적으로 파악하는데 매우 효율적인 것으로 평가되었다.

• 한국어류학회지
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• 제19권2호
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• pp.83-87
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• 2007

어류 에드워드감염증에 대한 예방 재조합 ghost 백신을 개발하기 위한 연구의 일환으로 어류 병원성 세균인 Edwardsiella trada를 대상으로 플라스미드 형질전환을 실시하고 형질전환 안정성을 평가하였으며, 형질 도입된 재조합 ghost 세균의 E-lysis 유전자 발현을 분석하였다. E. tarda를 대상으로 한 ghost 유도는 대장균에 비해 상대적으로 장시간의 반응 시간이 요구되며 lysis의 개시가 지연된 점을 고려 시 발현된 E protein의 용해 능력 또는 E-gene의 전사발현 양이 E. tarda에서 다소 약화되는 것으로 나타났다. 그러나 대장균에 비해 ghost 유도 속도가 다소 낮음에도 불구하고 반응이 완성되었을 시점에서의 E. tarda의 ghost 효율은 대장균과 전혀 차이가 없이 99.99% 이상의 유도효율을 나타내었다.

• Korean Journal of Mathematics
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• 제14권2호
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• pp.227-232
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• 2006

We study some dynamical properties in the context of bitransformation groups, and show that if (H,X,T) is a bitransformation group such that (H,X) is almost periodic and (X/H,T) is pointwise almost periodic $T_2$ and $x{\in}X$, then $E_x=\{q{\in}E(H,X){\mid}qx{\in}{\overline{xT}\}$ is a compact $T_2$ topological group and $E_{qx}=E_x(q{\in}E(H,X))$ when H is abelian, where E(H,X) is the enveloping semigroup of the transformation group (H,X).

• 한국균학회지
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• 제17권1호
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• pp.27-30
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• 1989

팽나무버섯 ${\beta}-isopropylmalate$ dehydrogenase의 유전자가 PBR322에 크로닝된 백터를 이용하여 사철느타리버섯 Leucine 영양요구성 균주를 형질전환 시켰다. 형질전환율은 저조하였으나 형질전환된 균총은 버섯 최소배지에서 다소 느리지만 생육할 수 있었다. 형질전환은 Southern hybridization과 형질 전환된 사철 느타리버섯 염색체 DNA를 다시 대장균에 형질전환시켜 확인하였다.

• 제어로봇시스템학회논문지
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• 제16권6호
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• pp.559-565
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• 2010

In this paper, we present a transformation method of source code from a POSIX (Portable Operating System Interface) based source code into an OSEK/VDX (Offene Systeme und deren Schnittstellen fur die Elektronik in Kraftfahrzeugen/Vehicle Distributed eXecutive) source code. As the electronic parts of automobile systems increase, the use of embedded software in automobile systems is also growing. Accordingly, many electronic systems are designed in automobile system with OSEK/VDX. Otherwise, one of the major problems of embedded software would be portability to other OS's. To enhance the portability and interoperability of embedded software, we propose a source code transformation method from POSIX to OSEK/VDX based on API (Application Programming Interface) translation method. Considering the characteristics of the OSEK/VDX which uses OIL (OSEK/VDX Implementation Language) standard, transformation process is performed with source code transformation and OIL code generation. For the validity of the proposed method, the transformation experiment is given using Micro-C OS II and OSEK/VDX with XC167CI micro-controller.

• 제어로봇시스템학회논문지
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• 제19권12호
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• pp.1145-1151
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• 2013

Kinematic modeling is a prerequisite for motion planning and the control of mobile robots. In this paper, we proposed a new method of kinematic modeling for a type of mobile robot based on differential motion transformation. The differential motion implies a small translation and rotation in three-dimensional space in a small time interval. Thus, transformation of the differential motion gives the velocity relationship, i.e., Jacobian between two coordinate frames. Since the theory of the differential motion transformation is well-developed, it is useful for the systematic velocity kinematic modeling of mobile robots. In order to show the validity for application of the differential motion transformation, we obtained velocity kinematic models for a type of exemplar mobile robot including spherical ballbots.

• 대한기계학회논문집
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• 제12권6호
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• pp.1296-1302
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• 1988

The study has been carried out under various experimental conditions to investigate mechanical properties by the transformation conditions of austempered low-alloy ductile cast iron. The amount of retained austenite and bainite after quenching was determined by the X-ray diffractometer and the point counting method and which the microstructure was investigated by the S.E.M. The mechanical properties of austempered low-alloy ductile cast iron can be varried over a comparatively wide range by changing the transformation conditions. During isothermal transformation of austenite in the bainite region, low-alloy ductile cast iron austempered at holding time of 40 minute has the maximum volume fraction(24%) of retained austenite in the cast iron matrix and which optimum values of mechanical properties correspond to the maximum amount of retained austenite, which falls with decreasing transformation temperature. The low values of both tensile strength and elongation in the initial stage of bainite transformation can be explained by premature fracture of tensile specimens and the tensile strength, hardness and elongation do not change considerably after a certain period. With a decreasing transformation temperature the tensile strength increase while the elongation decrease, especially the elongation has the maximum value at temperature $370^{\circ}C$.

• 전기학회논문지
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• 제59권9호
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• pp.1680-1685
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• 2010

In this note, a proof of Utkin's theorem is presented for a MI(Multi Input) uncertain linear case. The invariance theorem with respect to the two transformation methods so called the two diagonalization methods are proved clearly and comparatively for MI uncertain linear systems. With respect to the sliding surface transformation and the control input transformation, the equation of the sliding mode i.e., the sliding surface is invariant. Both control inputs have the same gains. By means of the two transformation methods the same results can be obtained. Through an illustrative example and simulation study, the usefulness of the main results is verified.

• Journal of applied mathematics & informatics
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• 제33권1_2호
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• pp.229-234
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• 2015

In the setting of semidefinite linear complementarity problems on $S^n$, we focus on the Stein Transformation $S_A(X):=X-AXA^T$ for $A{\in}R^{n{\times}n}$ that is positive semidefinite preserving (i.e., $S_A(S^n_+){\subseteq}S^n_+$) and show that such transformation is strictly monotone if and only if it is nondegenerate. We also show that a positive semidefinite preserving $S_A$ has the Ultra-GUS property if and only if $1{\not\in}{\sigma}(A){\sigma}(A)$.