Transformation of the ${\beta}-Isopropylmalate$ Dehydrogenase Gene of Flammulina velutipes into Pleurotus florida

팽나무버섯 Leu 2 gene(${\beta}-isopropylmalate$ dehydrogenase)의 사철 느타리버섯 형질전환

F. velutipes Leu 2 gene (${\beta}-isopropylmalate$ dehydrogenase gene) was used for transformation of P. florida leucine requiring auxotrophic mutant P101. Transformation frequency was very low but the transformed colony can grow on minimal medium very slowly. Transformation was identified by Southern hybridization and reverse transformation into E. coli using chromosome DNA isolated from transformed P. florida.

팽나무버섯 ${\beta}-isopropylmalate$ dehydrogenase의 유전자가 PBR322에 크로닝된 백터를 이용하여 사철느타리버섯 Leucine 영양요구성 균주를 형질전환 시켰다. 형질전환율은 저조하였으나 형질전환된 균총은 버섯 최소배지에서 다소 느리지만 생육할 수 있었다. 형질전환은 Southern hybridization과 형질 전환된 사철 느타리버섯 염색체 DNA를 다시 대장균에 형질전환시켜 확인하였다.