• Journal of Plant Biotechnology
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• 제3권3호
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• pp.113-121
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• 2001

The use of a marker gene in a transformation process aims to give a selective advantage to the transformed cells, allowing them to grow faster and better, and to kill the non-transformed cells. In general, the selective gene is introduced into plant genome along with the genes of interest. In some cases, the marker gene can be the gene of interest that will confer an agronomic characteristic, such as herbicide resistance. In this review we list and discuss the use of the most common selective marker genes on plant transformation and the effects of their respective selective agents. These genes could be divided in categories according their mode of action: genes that confer resistance to antibiotics and herbicides; and genes for positive selection. The contention of the marker gene flow through chloroplast transformation is further discussed. Moreover, strategies to recover marker-free transgenic plants, involving multi-auto-transformation (MAT), co-transformation, site specific recombination and intragenomic relocation of transgenes through transposable elements, are also reviewed.

• 대한수의학회지
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• 제24권1호
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• pp.40-49
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• 1984

To investigate the factors influencing the artifical transformation in Escherichia coli, E. coli C600 was transformed by pBR322 DNA with tetracycline and ampicillin resistant gene purified by CsCl-Etbr equilibrium density gradient centrifugation from E.coli HB 101. The influencing factors in the transformation such as concentration of calcium chloride, time of ice incubation, temperature and time of heat shock, time of gene expression, effects of plasmid DNA concentration and adding time were examined in these experiments. The results obtained were as follows; 1. The highest transformation frequency was observed in the treatments of 100 mM $CaCl_2$ before heat shock and the treatment of $CaCl_2$ was essential step in the process of E. coli transformation. 2. The highest transformation frequency was observed in the treatment of heat shock at $42^{\circ}C$ for 4 min. or $37^{\circ}C$ for 6 min., but the prolonged heat shock resulted a decreased transformation frequency. 3. Treatments of ice incubation at $0^{\circ}C$ for 45 min. before heat stocks or at $0^{\circ}C$ for 30min. after heat shock resulted an increased transformation frequency. 4. There was a linear relationship between DNA concentration and transformation frequency at the concentration of $8{\times}10^3$ recipient cells. The highest transformation frequency reached in carte of 7 mcg of donor DNA, but above 1 mcg of DNA concentration, transformation frequency was not remarkably increased. Addition of donor DNA just after the treatment of $CaCl_2$ was the best. 5. The best condition of gene expression at $37^{\circ}C$ were 40min. for TC-resistant gene and 100min. for AP-resistant gene. TC-resistant gene was higher in the transformation frequency and faster in the gene expression time than AP-resistant gene. In these results, the best conditions for the transformation of E. coli C 600 with pBR322 DNA were: treatment with 100mM $CaCl_2$, ice incubation at $0^{\circ}C$ for 45 min, heat shock at $42^{\circ}C$ for 4 min., 30 min. of ice incubation and incubation at $37^{\circ}C$ for 100min. for gene expression in that order.

• Journal of Plant Biotechnology
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• 제48권3호
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• pp.156-164
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• 2021

Spirodela polyrhiza, from the Lemnaceae family, are small aquatic plants that offer an alternative plant-based system for the expression of recombinant proteins. However, no turion transformation protocol has been established in this species. In this study, we exploited a pB7YWG2 vector harboring the eYFP gene that encodes enhanced yellow fluorescent protein (eYFP), which has been extensively used as a reporter and marker to visualize recombinant protein localization in plants. We adopted Agrobacterium tumefaciens-mediated turion transformation via vacuum infiltration to deliver the eYFP gene to turions, special vegetative forms produced by duckweeds to endure harsh conditions. Transgenic turions regenerated several duckweed fronds that exhibited yellow fluorescent emissions under a fluorescence microscope. Western blotting verified the expression of the eYFP protein. To the best of our knowledge, this is the first report of an efficient protocol for generating transgenic S. polyrhiza expressing eYFP via Agrobacterium tumefaciens-mediated turion transformation. The ability of turions to withstand harsh conditions increases the portability and versatility of transgenic duckweeds, favoring their use in the further development of therapeutic compounds in plants.

• 한국농공학회지
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• 제37권E호
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• pp.1-9
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• 1995

It is shown that two step power transformation is more efficient for the normalization of frequency distribution with the coefficient of skewness of zero in comparison with others including SMEMAX and power transformations. It is confirmed that the design low flows calculated using power and two step power transformations used in this study are generally nearer to the observed data as compared with those of SMEMAX transformation at all return periods in the applied watersheds of the Kum, Naktong and Yongsan rivers in Korea.

• 한국기록관리학회지
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• 제3권1호
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• pp.141-158
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• 2003

본고에서는 지식정보화의 발전에 따라 전개되는 새로운 현상과 변화들을 살펴보고 전통 행정과 뉴거버넌스의 비교를 통하여 전자정부의 의의를 도출하고, 전자정부의 모델을 제시하고 결론으로 차기 전자정부의 방향을 제시하였다. 새로운 현상과 변화로서는 제품과 서비스의 수렴형 모델로 변화, 사이버 주권의 딜레마 및 프라이버시와 정보보안의 위협 등을 들 수 있다. 한편 정부 주도적인 정부운영에서 NGO의 역할이 강화되는 뉴거버넌스로의 전환이 이루어지고 있으며 전자정부도 뉴거버넌스의 이념을 실현할 수 있도록 기여해야 한다. 이에 새로운 전자정부는 정부와 국민이 함께 구축 운영하는 체제로 전환되어야 한다. 이를 위해 공개키기반구조(PKI: Public Key Infrastructure)를 확산하고 전자민주주의(e-Democracy)의 활성화 및 국민이 용이하게 사용할 수 있는 사용자 인터페이스의 향상에 주력해야 한다.

• 전기의세계
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• 제13권2호
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• pp.1-4
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• 1964

Phase transformation is used to change some phase from phase in A.C. system. We have been used Scott or Fork connection in phase transformation the otherwise phase transformation was constructed from M-G set. From this M-G set, we could make phase shift facilities by mannual. Now, I can derive more easy phase transformation from taking another method. I believe this new phase transformation method in the first thing in the world. And so, I am going to explain about phase transformer construction process. The first, we could devide into equal part of core around the iron core as to be same size. The second, you will make primary and secondary winding on the core. The third, when you will supplied three phase A.C. at the terminal of primary winding you can get e.m.f. inducing of some phase at secondary. And so, we could make phase change from some phase A.C. We can apply this principle in many fields, i.e., freequency changer, phase leader of no use condenser, voltage regulator in keeping balance, and D.C. generator. And more, I will introduce in details concerning main pinciple and theory through following chapter.

• 충청수학회지
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• 제6권1호
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• pp.113-130
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• 1993

In this paper, we introduce certain subgroups $E^{Rel}_n$ (X, Y, K, G) of the relative homotopy groups ${\sigma}_n$(X, Y, K, G) of a transformation group and examine the algebraic properties of these groups.

• 충청수학회지
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• 제5권1호
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• pp.9-21
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• 1992

We define a certain subgroup $E_n$ (X, K, G) of the relative homotopy groups of a transformation group ${\sigma}_n$(X, K, G). The algebraic properties of these groups are examined.

• Journal of Microbiology and Biotechnology
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• 제22권9호
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• pp.1279-1287
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• 2012

We established an efficient transformation method for thermophile Geobacillus kaustophilus HTA426 using conjugative transfer from Escherichia coli of host-mimicking plasmids that imitate DNA methylation of strain HTA426 to circumvent its DNA restriction barriers. Two conjugative plasmids, pSTE33T and pUCG18T, capable of shuttling between E. coli and Geobacillus spp., were constructed. The plasmids were first introduced into E. coli BR408, which expressed one inherent DNA methylase gene (dam) and two heterologous methylase genes from strain HTA426 (GK1380-GK1381 and GK0343-GK0344). The plasmids were then directly transferred from E. coli cells to strain HTA426 by conjugative transfer using pUB307 or pRK2013 as a helper plasmid. pUCG18T was introduced very efficiently (transfer efficiency, $10^{-5}-10^{-3}\;recipient^{-1}$). pSTE33T showed lower efficiency ($10^{-7}-10^{-6}\;recipient^{-1}$) but had a high copy number and high segregational stability. Methylase genes in the donor substantially affected the transfer efficiency, demonstrating that the host-mimicking strategy contributes to efficient transformation. The transformation method, along with the two distinguishing plasmids, increases the potential of G. kaustophilus HTA426 as a thermophilic host to be used in various applications and as a model for biological studies of this genus. Our results also demonstrate that conjugative transfer is a promising approach for introducing exogenous DNA into thermophiles.

• The Journal of Advanced Prosthodontics
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• 제10권2호
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• pp.132-137
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• 2018

PURPOSE. The purpose of this study was to evaluate and compare three polishing systems on the surface roughness and phase transformation of monolithic zirconia. MATERIALS AND METHODS. 100 disk shaped specimens (10 mm diameter, 3 mm thickness) were fabricated from monolithic zirconia blocks. 20 specimens were left as a control group and remaining specimens were grinded by diamond bur to simulate the occlusal adjustments. Grinded specimens were randomly divided into 4 groups: group G (no polishing), group M (Meisinger, zirconia polishing kit), group E (EVE Diacera, zirconia polishing kit), and group P (EVE Diapol, porcelain polishing kit). Surface roughness was measured with profilometer and surface topography was observed with SEM. XRD analysis was performed to investigate the phase transformation. Statistical analysis was performed with one-way ANOVA and Tukey's post hoc tests at a significance level of P=.05. RESULTS. All polishing groups showed a smoother surface than group G. Among 3 polishing systems, group M and group E exhibited a smoother surface than the group P. However, no significant differences were observed between group M and group E (P>.05). Grinding and polishing did not cause phase transformations in zirconia specimens. CONCLUSION. Zirconia polishing systems created a smoother surface on zirconia than the porcelain polishing system. Phase transformation did not occur during the polishing procedure.