• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제9권2호
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• pp.205-212
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• 2003

최근 인터넷을 통한 B2B 전자상거래가 국제적으로 확대됨에 따라 단일화된 전자상거래 표준의 정립이 시급히 요구되고 있다. 이에 UN/CEFACT와 OASIS는 국제 단일 전자 시장 형성을 목적으로 ebXML 표준을 채택하였고, 새로운 표준을 따르는 전자상거래 시장에서는 메시징 서비스를 위하여 ebXML 기반 메시지 전송 시스템이 필요하게 되었다. 본 논문에서는 ebXML 메시징 서비스 제공을 위하여 ebXML 메시지를 생성하고 신뢰성있게 전송하며 관리하는 MSH(Message Service Handler)를 구현하였다. 구현한 MSH는 다양한 시스템 환 경을 지원하며, 단순한 인터페이스로 로컬 환경이나 분산 환경에서 MSH를 쉽게 사용할 수 있다. 또한 장애 발생시 메시지 재전송, 중복 메시지 처리, 확인(Acknowledgement) 메시지 전송 등을 통하여 인터넷 상에서 신뢰성 있는 메시지 전송을 보장한다. 개발된 시스템은 현재 ebXML 등록기/저장소 시스템에 적용되고 있다.

• Journal of Veterinary Science
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• 제22권6호
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• pp.76.1-76.15
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• 2021

Background: The development of a vaccine for Jembrana disease is needed to prevent losses in Indonesia's Bali cattle industry. A DNA vaccine model (pEGFP-C1-tat) that requires a functional delivery system will be developed. Polylactic-co-glycolic acid (PLGA) may have potential as a delivery system for the vaccine model. Objectives: This study aims to evaluate the in vitro potential of PLGA as a delivery system for pEGFP-C1-tat. Methods: Consensus and codon optimization for the tat gene was completed using a bioinformatic method, and the product was inserted into a pEGFP-C1 vector. Cloning of the pEGFP-C1-tat was successfully performed, and polymerase chain reaction (PCR) and restriction analysis confirmed DNA isolation. PLGA-pEGFP-C1-tat solutions were prepared for encapsulated formulation testing, physicochemical characterization, stability testing with DNase I, and cytotoxicity testing. The PLGA-pEGFP-C1-tat solutions were transfected in HeLa cells, and gene expression was observed by fluorescent microscopy and real-time PCR. Results: The successful acquisition of transformant bacteria was confirmed by PCR. The PLGA:DNA:polyvinyl alcohol ratio formulation with optimal encapsulation was 4%:0.5%:2%, physicochemical characterization of PLGA revealed a polydispersity index value of 0.246, a particle size of 925 nm, and a zeta potential value of -2.31 mV. PLGA succeeded in protecting pEGFP-C1-tat from enzymatic degradation, and the percentage viability from the cytotoxicity test of PLGA-pEGFP-C1-tat was 98.03%. The PLGA-pEGFP-C1-tat demonstrated luminescence of the EGFP-tat fusion protein and mRNA transcription was detected. Conclusions: PLGA has good potential as a delivery system for pEGFP-C1-tat.

• 한국정보과학회논문지:정보통신
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• 제29권1호
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• pp.15-23
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• 2002

본 논문에서는 3GPP2 IMT-2000 서비스를 위해 기존 음성 및 인터넷 인프라를 수용할 수 있으며, IMT-2000망에서 Mobile-IP over IMT-2000 멀티미디어 응용 서비스를 제공하는데 필수적인 MDA(Multicast Delivery Agent)-기반의 Mobile-IPv6 멀티캐스트 알고리즘을 제안하고 이를 분석한다. IMT-2000 시스템 모델은 PDSN(Packet Data Serving Node) 또는 IWF(Interworking Function)기능이 각 기지국에 존재하는 분산된 모델을 사용한다. 새로운 프로토콜의 핵심요소는 멀티캐스트 데이터그램 전달 경로를 줄이기 위해 MDA (Multicast Delivery Agent)를 도입한 것이다. 제안된 프로토콜은 현재까지 제안된 이동망에서 멀티캐스트 지원 기법들과 비교하여, 짧은 라우팅 경로 및 이동 호스트에 근접한 노드에서 이동 호스트로의 직접 데이터그램 전송을 통하여 성능 향상을 기대할 수 있다. 특히, 터널링 수 및 멀티캐스트 데이터그램의 평균 라우팅 길이가 감소하고, 멀티캐스트 데이터그램의 트래픽 부하도 감소한다.

• 한국환경보건학회지
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• 제23권4호
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• pp.50-56
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• 1997

Applied for Drug Delivery System, polymer drug was prepared with chitosan and phthalylsulfathiazole. In spite of various application of chitin derivatives, commercial use of chitin has been limited due to highly resistance to chemicals and the absense of proper solvents. In this study, Chitosan were prepared from chitin which were deacetylated under various condition. The synthetic procedures of polymer drug were performed by acid chloride methods. The antibiotic activities of polymer drug exhibited growth-inhibitory activity against Staphylococcus aureus, Staphylococcus epidermidis, E. coli, Salmonella typhimurium, Klebsiella pneumoniae at the concentration of 471-514 $\mu$g/ml in general. Comparatively, Polymer drug exhibited broad antibacterial activity on MICs 897-1280 $\mu$g/ml against Gram-positive and Gram-negative bacteria including Staphylococcus aureus, Staphylococcus epidermidis and E. coli.

• Archives of Pharmacal Research
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• 제29권12호
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• pp.1164-1170
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• 2006

A triptolide-lysozyme (TP-LZM) conjugate was synthesized to achieve renal specific delivery and to reduce the side effects of triptolide. Triptolide was coupled to lysozyme through succinic via an ester bond with an average coupling degree of 1 mol triptolide per 1 mol lysozyme. The lysozyme can specifically accumulate in the proximal tubular cells of the kidney, making it a potential carrier for targeting drugs to the kidney. The structure of triptolide succinate (TPS) was confirmed by IR, $^{1}H-NMR$, MS and UV. The concentrations of triptolide in various samples were determined by reversed-phase high-performance liquid chromatography (HPLC). In this study, the physicochemical and stability profiles of TP-LZM under various conditions were investgated the stability and releasing profiles of triptolide-lysozyme (TP-LZM) under various conditions. In vitro release trails showed triptolide-lysozyme was relatively stable in plasma (less than 30% of free triptolide released) and could release triptolide quickly in lysosome (more than 80% of free triptolide released) at $37^{\circ}C$ for 24 h. In addition, the biological activities of the conjugate on normal rat kidney proximal tubular cells (NRK52E) were also tested. The conjugate can effectively reduce NO production in the medium of NRK52E induced by lipopolysaccharide (LPS) but with much lower toxicity. These studies suggest the possibility to promote curative effect and reduce its extra-renal toxicity of triptolide by TP-LZM conjugate.

• 디지털융복합연구
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• 제6권1호
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• pp.1-10
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• 2008

Electronic service delivery is an integral mission that is shouldered upon e-government to carry out. And it also has become an important way of making client-centered administration work more effectively. It is quite convenient from citizens' perspective to utilize public services that are electronically transformed. This study identifies a set of electronic services currently provided by e-government and proposes policy measures for further driving e-government to develop its services in a direction of making people's life more convenient.

• Carbon letters
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• 제11권2호
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• pp.122-126
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• 2010

The alginate-based hydrogel was prepared as a pH-sensitive drug delivery system. To enhance the drug loading capacity, activated carbon was introduced as a drug absorbent. The iron oxide was incorporated into the alginate matrix for the magnetic transferring to the target organ. The activated carbon and iron-oxide were dispersed uniformly in the alginate hydrogel. The drug release from the alginate/activated carbon composite hydrogel was carried out in various pH conditions with vitamin B12 and Lactobacillus lamnosers as model drugs. The fast and sustainable release of drug was observed in the basic condition due to the pH-sensitive solubility of alginate. The novel drug delivery system having pH-sensitive release property and magnetic movement to target place was developed by using the alginate/activated carbon composite magnetic hydrogels.

• 산업공학
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• 제12권4호
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• pp.557-566
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• 1999

This paper considers a door-to-door service system in which pickups or deliveries are performed by a trip of a single vehicle. Each customer request specifies the quantity of the load transported, the location, and the time window within which it is to be picked up or delivered. Since the system is demand responsive, i.e., new or emergent requests become available in real-time, the current vehicle route has to be reconstructed to include these requests. In this case, only continuous vehicle tracking enables control over the requests and ensures that the requests are satisfied on time. This paper suggests a pilot pickup/delivery management system integrating a geographic information system(GIS) and a global position system(GPS) to efficiently deal with such a dynamic environment. The GIS offers a way of displaying the vehicle route on digital maps for the region under concerned. Also displayed is the current location of the vehicle obtained from the GPS. A heuristic algorithm is used to dynamically determine the vehicle route. A practical example is provided to show the feasibility of the system.

• Journal of Pharmaceutical Investigation
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• 제29권2호
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• pp.111-115
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• 1999

We have studied the transdermal flux of prostaglandin $E_1$ $(PGE_1)$ from a hydrogel patch through hairless mouse skin, to test the possibility of developing a transdermal delivery system. Karaya gum patch containing $PGE_1$ was prepared by casting method. $PGE_1$ was stable in the patch for 10 weeks. The effect of current application, enhancer (propylene glycol monolaurate : PGML), adhesive and patch thickness on the flux was studied using side-by-side diffusion cell. Passive flux of $PGE_1$ was negligible. Cathodal delivery increased the flux about 20 fold. As the concentrations of PGML increased, flux increased. When 5% PGML was used as the enhancer, maximum flux by cathodal iontophoresis was $55\;{\mu}g/cm^2\;hr$. It increased about 2 folds to $100\;{\mu}g/cm^2\;hr$, when the amount of PGML used was 9%. Large increase in flux and the decrease in time to reach maximum flux were observed when the skin was pretreated with neat PGML (maximum flux obtained was about $200\;{\mu}g/cm^2\;hr$). Use of adhesive decreased the flux significantly. To the contrary of our expectation, increase in current density decreased the flux. These flux data together with the stability data indicate that, though the onset of sufficient delivery occur after 1-2 hours of application, therapeutic amount of $PGE_1$ can be delivered through skin using iontophoresis and penetration enhancer.

• 한국융합학회논문지
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• 제10권7호
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• pp.115-120
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• 2019

본 연구는 국내 이커머스 시장의 신선식품 배송 패키지 디자인이 발전해야 할 방향성을 제시하는 데 그 목적이 있다. 1차로 문헌연구를 통해 이커머스 식료품과 관련된 소비 트렌드, 콜드체인 시스템, 제로 웨이스트 캠페인에 관하여 이론을 정리하였다. 그리고 국내외 신선식품 배송 패키지에 대해 분석하여 현황을 파악하고 관련 선행연구를 통해 앞으로의 방향성에 대해 알아보았다. 연구 결과, 신선식품을 위한 배송 패키지 디자인은 신선도 유지뿐만 아니라 포장재의 유용성, 편리성과 환경보호 측면을 다각도로 고려하여야 한다. 이커머스 기업은 상품과 함께 패키지를 통해 '신선함'이라는 경험을 전달할 때, 소비자는 오프라인보다 높은 구매율과 충성도를 보여줄 것으로 기대한다.