• Journal of Microbiology
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• 제44권3호
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• pp.293-300
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• 2006

The Escherichia coli heat-labile enterotoxin B subunit (HLT-B) is one of the most powerful mucosal immunogens and known mucosal adjuvants. However, the induction of high levels of HLT-B expression in E. coli has proven a difficult proposition. Therefore, in this study, the HLT-B gene was cloned from pathogenic E. coli and expressed as a fusion protein with GST (glutathion S-transferase) in E. coli BL2l (DE3), in an attempt to harvest a large quantity of soluble HLT-B. The culture conditions, including the culture media used, temperature, pH and the presence of lactose as an inducer, were all optimized in order to obtain an increase in the expression of soluble GST-rHLT-B. The biological activity of the purified rHLT-B was assayed in a series of GMI-ELISA experiments. The findings of these trials indicated that the yield of soluble recombinant GST-rHLT-B could be increased by up to 3-fold, as compared with that seen prior to the optimization, and that lactose was a more efficient alternative inducer than IPTG. The production of rHLT-B, at 92 % purity, reached an optimal level of 96 mg/l in a 3.7 L fermentor. The specific GM1 binding ability of the purified rHLT-B was determined to be almost identical to that of standard CTB.

• 한국컴퓨터정보학회논문지
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• 제23권1호
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• pp.91-102
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• 2018

The purpose of this study is to develop an excellent bodice prototype that is adapted to the body shape of middle-aged women using 3D measurement data. In the evaluation of appearance, S pattern 4.00, B pattern 2.80, E pattern 2.40, L pattern 1.40 were shown in order, and the best fit of S pattern was evaluated as excellent. As a result of looking at the color distribution chart to find out the amount, E pattern and S pattern were not space in the front bust, armhole, and the back waist line. The B pattern and the L pattern were marked in blue because of insufficiency space in the back neck. As a result of evaluation the amount of air gap in the clothing, the air gap of the bust was 0.12, which is the largest pattern of B. Next, the L pattern appears as a tight circle with smallest air gap in the order of the S pattern 0.096, the E pattern 0.08, and the L pattern 0.003. The S pattern was evaluated to be the most appropriate for the body shape of middle-aged women. But the waist and back were slightly tight. Middle-aged women have larger shoulder-related items and larger waist circumference. Therefore, when you set the perimeter item, you should add 1-2cm of space amount and give extra space to the circumference area.

• Journal of Positioning, Navigation, and Timing
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• 제9권4호
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• pp.327-336
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• 2020

This study presents signal availability of inter-operable global navigation satellite system (multi-GNSS) combined with future Korean Positioning System (KPS), specifically at geosynchronous orbit (GSO). The orbit of KPS, which is currently under conceptual feasibility study, is first introduced, and the grid points for evaluating space service volume (SSV) at GSO are generated. The signal observabilities are evaluated geometrically between those grid points and KPS/GNSS satellites. Then, analyzed are the visibility averaged over time/space and outage time to not access one or four signals. The reduction of maximum outage time induced by KPS are presented with different maximum off-boresight angles depending on L1/E1/B1 and L5/L3/E5a/B2 frequencies. Our numerical analysis shows that the SSV of multi-GNSS combined with KPS provides up to 7 additional signals and could provide continuous observation time (zero outage time) of more than four GNSS or KPS signals for 3.20-14.83% of SSV grid points at GSO. Especially at GSO above North/South America and Atlantic region, the introduction of KPS reduces the outage duration by up to 63 minutes with L1/E1/B1 frequency.

• 대한수학회논문집
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• 제24권3호
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• pp.367-379
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• 2009

For a prime number p, let $\mathbb{Q}_p$ denote the p-adic field and let $\mathbb{Q}_p^d$ denote a vector space over $\mathbb{Q}_p$ which consists of all d-tuples of $\mathbb{Q}_p$. For a function f ${\in}L_{loc}^1(\mathbb{Q}_p^d)$, we define the Hardy-Littlewood maximal function of f on $\mathbb{Q}_p^d$ by $$M_pf(x)=sup\frac{1}{\gamma{\in}\mathbb{Z}|B_{\gamma}(x)|H}{\int}_{B\gamma(x)}|f(y)|dy$$, where |E|$_H$ denotes the Haar measure of a measurable subset E of $\mathbb{Q}_p^d$ and $B_\gamma(x)$ denotes the p-adic ball with center x ${\in}\;\mathbb{Q}_p^d$ and radius $p^\gamma$. If 1 < q $\leq\;\infty$, then we prove that $M_p$ is a bounded operator of $L^q(\mathbb{Q}_p^d)$ into $L^q(\mathbb{Q}_p^d)$; moreover, $M_p$ is of weak type (1, 1) on $L^1(\mathbb{Q}_p^d)$, that is to say, |{$x{\in}\mathbb{Q}_p^d:|M_pf(x)|$>$\lambda$}|$_H{\leq}\frac{p^d}{\lambda}||f||_{L^1(\mathbb{Q}_p^d)},\;\lambda$ > 0 for any f ${\in}L^1(\mathbb{Q}_p^d)$.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2007년도 추계종합학술대회
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• pp.863-867
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• 2007

본 논문은 Fractal 구조에 기초로 한 L-형 급전구조를 이용한 4세대 이동통신용 소형 마이크로스트립 패치 안테나를 설계하였다. 소형 안테나는 "Crossed-diagonal"라 불리는 특이한 형태의 공진 흐름이 존재함으로써 얻을 수 있다. 설계는 CST Microwave Studio 5.0으로 하였으며, 설계된 패치 안테나의 동작 특성으로서 입력 반사손실이 -10 [dB]이하 VSWR 2:1인 범위가 2.944 [GHz] ${\sim}$ 4.209 [GHz]까지 1.2647[GHz]의 35.4%라는 특성을 나타내었고, E-평면과 H-평면의 이득은 8.3dBi, 8.4dBi 그리고 3dB 빔폭은 E-평면에서 $40.6^{\circ}$ H-평변에서 $81.6^{\circ}$라는 시뮬레이션 결과를 각각 얻을 수 있었다.

• 대한치과기공학회지
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• 제37권4호
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• pp.199-203
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• 2015

Purpose: The purpose of this in vitro study was to evaluation the effect of shade on all ceramic restoration based zirconia according to cooling rate on Firing. Methods: 10 specimens applied to the dentin porcelain were made on the zirconia. After 5 specimens of the first group were burned in the furnace, these were put out rapidly. And after 5 specimens of the second group were burned in the furnace, these were put out slowly later 15 min. All specimens were measured $L^*$, $a^*$, $b^*$ using spectrophotometer, there were calculated 10 mean(SD) of descriptive statistics with SPSS program. These data were used for ${\Delta}E^*$ with color difference equation. Independent t tests were performed between 2 groups. Results: $L^*$, $a^*$, $b^*$ of 2 groups was statistically significant respectively (p<0.001), ${\Delta}E^*$ was 4.55 value. Conclusion: This study showed effect of shade on all ceramic restoration based zirconia according to cooling rate on Firing.

• Journal of Microbiology
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• 제42권2호
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• pp.111-116
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• 2004

It is known that Bacillus subtilis glutamyl-tRNA synthetase (GluRS) mischarges E. coli $tRNA_{1}$$^{Gln}$ with glutamate in vitro. It has also been established that the expression of B. subtilis GluRS in Escherichia coli results in the death of the host cell. To ascertain whether E. coli growth inhibition caused by B. subtilis GluRS synthesis is a consequence of Glu-$tRNA_{1}$$^{Gln}$ formation, we constructed an in vivo test system, in which B. subtilis GluRS gene expression is controlled by IPTG. Such a system permits the investigation of factors affecting E. coli growth. Expression of E. coli glutaminyl-tRNA synthetase (GlnRS) also amelio-rated growth inhibition, presumably by competitively preventing $tRNA_{1}$$^{Gln}$ misacylation. However, when amounts of up to 10 mM L-glutamine, the cognate amino acid for acylation of $tRNA_{1}$$^{Gln}$, were added to the growth medium, cell growth was unaffected. Overexpression of the B. subtilis gatCAB gene encoding Glu-$tRNA^{Gln}$ amidotransferase (Glu-AdT) rescued cells from toxic effects caused by the formation of the mis-charging GluRS. This result indicates that B. subtilis Glu-AdT recognizes the mischarged E. coli Glu-$tRNA_{1}$$^{Gln}$, and converts it to the cognate Gln-$tRNA_{1}$$^{Gln}$ species. B. subtilis GluRS-dependent Glu-$tRNA_{1}$$^{Gln}$ formation may cause growth inhibition in the transformed E. coli strain, possibly due to abnormal protein synthesis.

• 한국콘텐츠학회논문지
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• 제16권2호
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• pp.772-781
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• 2016

본 연구는 상악 전치부 색조 선택에 객관적인 기준을 설정하고자 색체계 Shade-Eye NCC를 이용하여 CIE $L^*$, $a^*$, $b^*$값으로 측정하여 이 값들을 비교 분석하고 그 의미를 고찰하고자 실시되었다. 치과를 내원하는 환자에게 연구 의도를 설명해 주고 동의를 얻어 111명(남자 50명, 여자 61명)의 3개 치아, 즉 상악 중절치, 상악 측절치, 상악 견치 총 333개의 치아를 측색하였다. 절단부와 치경부의 색차를 비교한 결과 상악견치의 ${\Delta}E^*$값이 $5.81({\pm}2.98)$으로 가장 낮았으며, 상악 측절치 $6.51({\pm}3.23)$, 상악 중절치의 $7.51({\pm}3.04)$ 순으로 점차 그 색차가 커지는 것을 알 수 있었다. 중절치와 측절치, 견치 모두 여성이 남성에 비해 명도($L^*$)가 높았으며, 황색채도($b^*$)는 중절치에서 남성이 여성보다 약간 높게 나타났다(p<0.05). 연령은 중절치의 명도($L^*$),적색채도($a^*$), 황색채도($b^*$), 측절치와 견치의 명도($L^*$), 황색채도($b^*$)에 유의미한 영향을 미치는 것으로 나타났다(p<0.05). 흡연은 자연치의 색조에 유의미한 영향을 미치지 못하였으며, 음주는 중절치의 명도($L^*$)를 감소시키며, 측절치의 적색채도($a^*$)를 감소시키는 경향을 나타냈다(p<0.05). 만성질환은 중절치와 측절치의 명도($L^*$)를 감소시키는 경향을 나타냈다(p<0.05).

• 한국산학기술학회논문지
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• 제20권10호
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• pp.62-67
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• 2019

본 연구는 rotifer Brachionus rotundiformis의 유성생식을 유도하기 위해 효과적인 매개물질로 몇 가지 성 steroid hormone (serotonin, progesterone 및 ${\beta}$-estradiol)을 선정하여 농도별(Low, 30 mg/L 및 High, 300 mg/L)로 내구란 생산효과를 6일 동안 배양하면서 조사하였다. 배양 3일째 ${\beta}$-estradiol ($E_2$) 처리구가 다른 실험구에 비해 유성생식률이 20.6%로 가장 높게 나타났다. 배양 6일째 내구란 생산수(106 개)도 동일한 결과를 보였으며 다음으로는 serotonin이 높게 나타났다. 더하여, 위 스테로이드 호르몬이 몇 가지 유성생식관련 유전자의 발현에 미치는 영향에 대해 조사한 결과, NrbP, SRY, Cyclin 및 MrpmB 유전자가 처리한 모든 hormone에 대해 up-regulation되는 경향을 보였다. 결과를 종합해보면, B. rotundiformis의 내구란 생산에 가장 효과적인 steroid hormone은 ${\beta}$-estradiol인 것으로 판단되며 유성생식 관련 유전자로 NrbP, SRY, MrpmrB 및 Cyclin을 제안할 수 있다. 차후 효과적인 내구란 생산을 위한 $E_2$의 최적 처리농도규명에 관한 추가 연구가 필요할 것으로 판단된다.

• Bulletin of the Korean Chemical Society
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• 제9권3호
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• pp.115-117
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• 1988

The addition of L-cysteine without blocking amino and carboxyl groups to${\beta},{\beta}$-dinitrostyrene derivatives(11a-e) were investigated. ${\beta},{\beta}$ -Dinitrostyrene derivatives(11a-e) easily undergo addition reactions with L-cysteine to from s-(2,2-dinitro-1-phenylethyl)-L-cysteine(12a), s-[2,2-dinitro-1-(p-methyl)phenylethyl]-L-cysteine (12b), s-[2,2-dinitro-1-(p-methoxy)phenylethyl]-L-cystein e(12c), s-[2,2-dinitro-1-(p-chloro)phenylethyl]-L-cysteine (12d) and s-[2,2-dinitro-1-(p-nitro)phenylethyl]-L-cysteine( 12a), respectively. The structure of adducts were confirmed by means of spectral data, molecular weight measurement and elemental analysis.