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Efficacy of formalin bath against gill infections with Pseudodactylogyrus spp. in cultured eel Anguilla japonica (포르말린 약욕이 뱀장어 아가미흡충의 구제에 미치는 효과)

  • Jung, Sung-Hee;Jee, Bo-Young;Kim, Jin-Do;Seo, Jung-Soo;Kim, Jin-Woo
    • Journal of fish pathology
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    • v.23 no.3
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    • pp.293-302
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    • 2010
  • Effects of formalin on removal of Pseudodactylogyrus spp. were examined against naturally infected eel, Anguilla japonica (weight 89.9~96g) at a water temperature of $28^{\circ}$. Prior to experiments for removal of the parasite, the hematological toxicity of formalin bath at 0~500 ppm for 30 min~24 h was assessed by hematocrit values (Ht). Based on the results of Ht, appropriate methods of treatment, concentrations and durations, were examined in the main study. There was no significant (P>0.05) change of Ht in 100 to 200 ppm for 24 h. In contrast, Ht increased significantly (P<0.05) at above 300 ppm. This suggests that physiological damage was caused by formalin bath treating with 300 to 500 ppm. Formalin bath with 100 and 200 ppm for 24 h caused significant decreases (P<0.05) in the infection of the parasite. In conclusion, the 100 ppm formalin for 24 hour-bath was found most recommendable for the effective treatment of Pseudodactylogyrus spp. for the gills of the infected eel because of the median lethal concentration ($LC_{50}$) of formalin to eel; cumulative mortalities were found to be 0 and 13.3%, respectively, following 24 h bathing.

Determination of operating offline detention reservoir considering system resilience (시스템 탄력성을 고려한 빗물저류조 운영수위 결정)

  • Lee, Eui Hoon;Lee, Yong Sik;Jung, Donghwi;Joo, Jin Gul;Kim, Joong Hoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.10
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    • pp.403-411
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    • 2016
  • Recently, the number of occurrences of inundation and the severity of flood damage has increased rapidly as the frequency of localized heavy rainfall and the ratio of impervious area increased in urban areas. Most local governments focus on employing structural measures (e.g., the construction of detention reservoirs/pump stations, rehabilitation of drainage and sewer pipes) to prevent urban inundation. On the other hand, the effectiveness of implementing such structural measures is being dimished because there are already many inundation prevention facilities. The limitation of structural measures can be overcoming by employing non-structure measures, such as flood alerts and the operation of drainage facilities. This study suggests the pump operation rule (i.e., suggesting pump stop level) for a new detention reservoir operating method, which triggers the operation of a pump based on the water level at the monitoring node in urban drainage system. In the new reservoir operation, a total of 48 rainfall events are generated by the Huff distribution for determining the proper pump stop level. First, the generated rainfall events are distributed as frequencies, quartiles, and durations. The averaged system resilience value was determined to range from 1.2 m to 1.5 m is based on the rainfall-runoff simulation with rainfall generated by the Huff distribution. In this range, 1.2 m was identified considering the safety factor of 1.25 by the Standard on sewer facilities in 2011.

Clinical Features of Cholestatic Hepatitis (담즙정체성 간염의 임상적 양상)

  • Choi, Sun-Taek;Eun, Jong-Ryul;Lim, Song-Woo;Kim, Bong-Jun;Lee, Heoon-Ju;Gu, Mi-Jin;Choi, Joon-Hyuk
    • Journal of Yeungnam Medical Science
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    • v.18 no.1
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    • pp.51-58
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    • 2001
  • Background: Cholestatic hepatitis is failure of bile to reach the duodenum with hepatocellular damage and no demonstable obstruction of the major bile ducts. The prognosis is usually good with recovery in less than 4 weeks after withdrawal of the offending drug. However, a prolonged course lasting over 3 months is possible and, in rare cases, progression to ductopenia with development of a vanishing bile duct syndrome occurs. A differential diagnosis with other causes of Chronic liver disease is needed. Materials and Methods: From January 1991 through January 2000, 14 patients diagnosed as cholestatic hepatitis by liver biopsy were included. The possible causative drug, clinical features, laboratory findings, and progression of cholestatic hepatitis were evaluated. The semiquantitative study of liver lesions was performed by two independent observers. Results: Causes of cholestatic hepatitis are 5 cases of oriental medicine, 3 cases of anti-tuberculosis medication, 1 case of ticlopidine and antibiotics and 4 cases of unknown causes. The clinical features of cholestatic hepatitis were jaundice, itching, urine color change, and general weakness. During 6 to 30 months, LFT of 5 patients showed prolonged elevation. Elevated total cholesterol ${\geq}$250 mg/dL in 6 patients, pheripheral blood eosinophilia in 5 patients, auto-antibody positive in 6 patients were observed respectively. The biopsies showed intralobular bilirubinostasis with a mixed portal inflammatory infiltration. Conclusion: In cholestatic hepatitis, durations of abnormal LFT are variable regardless of causative drugs. If cholestatic hepatitis progresses toward chronic course, viral hepatitis, primary biliary cirrhosis, and autoimmune hepatitis should be differentially diagnosed and sequential liver biopsies are needed.

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Effects of Gamma-ray and Chemical Mutagens on the Germination and Seedling Growth in Stevia rebaudiana Bert. (감마선 및 화학적 돌연변이원 처리가 스테비아 (Stevia rebaudiana Bert.)의 종자 발아 및 초기 생장에 미치는 영향)

  • Yoon, Tai-Young;Kim, Ee-Youb;Kim, Young-Ho;Choi, Gin-Su;Hyun, Kyung-Sup;Seong, Yoon-Hee;Jo, Han-Jig;Kim, Dong Sub;Kang, Si-Yong;Ko, Jeong-Ae
    • Journal of Radiation Industry
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    • v.6 no.2
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    • pp.189-197
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    • 2012
  • This study was carried out to develop the improved useful mutants for yield or composition of stevia plants using the gamma ray or chemical mutagens treatments. The seeds of stevia 'Suwon No. 11' were irradiated up to 400 Gy of gamma ray. Chemical mutagens were treated on the seeds of the 'Suwon No. 11' using 0.07% colchicine, 10 mM sodium azide, or 10 mM NMU for various durations. The germination rate, and shoot and root growth of seedling were estimated at 30 days after gamma ray irradiation or chemical mutagen treatment, and the plant height, the number of branches, and leaf length and width were examined at 3 months after mutagenesis treatments. In the case of gamma ray treatments, the germination rate and early-stage growth were decreased as the increase of radiation dose, and the 50% lethal dose was found to be 200 Gy. the plant height was decreased as the increase of radiation dose, while the number of branches per plant and leaf length were increased. Leaf shape was modified to the relatively longer one compared to the control, which was identified more apparently at the treatments of higher than 150 Gy. In the treatment of chemical mutagens, the rate of germination and survival were decreased as the increase of incubation time. The 50% lethal dose for germination rate were identified as the conditions of the 15 hours incubation in 0.07% colchicine, the 4 hrs in 10 mM sodium azide, and the 2 hrs in 10 mM NMU, in the three chemical mutagens treatments. Chemical mutagens had no influence on shoot growth, while root growth was increased, especially as the incubation time was extended. The highest root growth occurred in the NMU treatment at 6 hrs incubation time. The plant height was decreased as the increase of incubation time in the chemical mutagens treatments. Among the chemical mutagens, NMU was the most effective to induce the mutants with long-shaped or the least lobed leaves.

Community structure of sessile organisms on PVC plates according to different submerged timings and durations in Jangmok Bay, Korea (남해 장목만에서 PVC판 투입시기와 투입기간에 따른 부착생물군집 구조)

  • Park, So-Hyun;Seo, Jin-Young;Choi, Jin-Woo
    • The Korean Journal of Malacology
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    • v.27 no.2
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    • pp.99-105
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    • 2011
  • This study was conducted to compare the species composition of sessile organisms on the artificial substrates of PVC submerged at different time intervals and duration in Jangmok Bay, Geoje Island, southern coast of Korea. Three PVC plates were submerged at one month interval from March to October and retrieved in November, 2007. A mussel, Mytilus galloprovincialis exclusively occupied the artificial substrates submerged from March to April and occurred as a dominant species to July. An ascidian, Styela plicata occurred as a dominant sessile species from May to August. Balanus amphtrite, Bugula sp., and hydrozoans occurred as dominant species on the plates submerged from July to September. There was a mis-match between the peak time of settlement and dominance of sessile organisms due to the interspecies competitions when the PVC plates were retrieved in November. There was no clear relationship between submerged duration and the abundance of sessile organisms due to the different settlement period. M. galloprovincialis seemed to be a strong competitor which could exclude the previous recruiters of macroalgae by overgrowth and occupy the substrate surface and maintain its high population density by preventing the settlement of other species until late autumn. These results suggested that the composition of sessile organisms in vacant hard substrates could be determined by the combined effects of supply-timing of larvae and post-settlement competitions.

Effects of Roasted Korean Oak Chip Addition on the Aging of Red Wine (국산 참나무편의 첨가가 적포도주의 숙성에 미치는 영향)

  • Lee, A-Rong;Park, Heui-Dong
    • Food Science and Preservation
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    • v.18 no.6
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    • pp.891-897
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    • 2011
  • Oak barrel have been used for a long time for the aging of wine. Instead of oak barrel aging, however, roasted oak chips can be directly added in the wine before aging. In this study, Korean oak chips roasted for different time durations were used for the red wine aging, and the properties of wine aged through the addition of the roasted oak chips were characterized. No significant differences were observed in the alcohol, soluble-solid, reducing-sugar and total-acid contents based on the oak chips aging, but the total phenol content increased. The methanol, ethyl acetate, propanol, isobutanol and isoamyl alcohol contents also increased. Especially, the methanol and propanol contents increased as the roasting time was prolonged. Although the hue values were similar to one another, the intensity as well as the lightness, redness, and yellowness values were high in the wine aged with the roasted oak chips. In the sensory evaluation, the wine aged with oak chips roasted for 2 h showed the highest scores in the flavor, taste, and overall preference.

Analysis of the Variability of Annual Precipitation According to the Regional Characteristics (지역특성별 연강수특성 변화분석)

  • Kim, Gwang-Seob;Kim, Jong-Pil;Lee, Gi-Chun
    • Journal of the Korean Society of Hazard Mitigation
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    • v.11 no.2
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    • pp.113-125
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    • 2011
  • In this study, recent trends of the annual precipitation, the annual maximum precipitation of different durations and the rain days over several thresholds(i.e. 0, 10, 20, 40, 60 and 80 mm/day) according to the different local features were analyzed using daily precipitation data of 59 weather stations between 1973 and 2009. To analyze the variability according to the regional characteristics, 59 weather stations were classified by elevations, latitudes, longitudes, river basins, inland or shore(east sea, south sea, west sea) area and the level of urbanization. Results demonstrated that overall trend of variables increases except rain day. Results according to the regional characteristics showed that the increase trend becomes stronger with elevation increase. The increase trend of Han river basin is largest and that of Youngsan river basin is smallest. Also the increase trend becomes stronger with latitude increase and that of East coast is larger than that of South coast since it may be caused by the regional difference of elevation. The increase trend of urban area is larger than that of rural area. Overall trend showed that increase trend becomes stronger with elevation and latitude increase.

Comparison of Economical Character of the Silkworm, Bombyx Mori L., Introduced from Foreign Sericultural Countries to Turkey (터어키에 도입된 잠품종의 실용형질의 비교에 관한 연구)

  • Ryu, Keun-Sup
    • Journal of Sericultural and Entomological Science
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    • v.21 no.1
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    • pp.46-49
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    • 1979
  • These experiments were made in order to compare the productivity of various varieties of F$_1$silkworm eggs imported from foreign sericultural countries with the productivity of some F$_1$silkworm eggs varieties produced by Turkey. The results are summarized as follows: 1. In the durations of whole instar, it lasted 30∼38 days under natural rearing conditions but Jam 107${\times}$Jam 105 about 30 days. 2. Cocoon yield per case of silkworm eggs was shown significantly among the varieties. Thaiei ${\times}$ Choan was the highest yield of cocoon, followed by Isonzo, Piave, M-198, M${\times}$N, S${\times}$P, J${\times}$C and Jam 107${\times}$Jam 108 in that order. 3. In the percentage of cocoon shell, M-197 was the highest and other varieties were almost on the same level of cocoon shell, but Jam 107${\times}$ Jam 108 was the lowest percentage. 4. In the weight of a cocoon, Choan${\times}$Thaiei was the heaviest, followed by S${\times}$P, C${\times}$J, M${\times}$N, M-197, Piave, Livenzo and Jam 107${\times}$Jam 108 in that order. 5. In the raw silk percentage of fresh cocoon, Choan${\times}$Thaiei, 3 Italian varieties and Turkish M${\times}$N. C${\times}$J belonged to the first group of better raw silk percentage, followed Polish M-197 and Jam 107${\times}$1am 108 in that order.

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Effects of method and duration of restraint on stress hormones and meat quality in broiler chickens with different body weights

  • Ismail, Siti Nadirah;Awad, Elmutaz Atta;Zulkifli, Idrus;Goh, Yong Meng;Sazili, Awis Qurni
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.6
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    • pp.865-873
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    • 2019
  • Objective: The study was designed to investigate the effects of restraint method, restraint duration, and body weight on stress-linked hormones (corticosterone, adrenaline, and noradrenaline), blood biochemical (namely glucose and lactate), and the meat quality in broiler chickens. Methods: A total of 120 male broiler chickens (Cobb 500) were assigned to a $2{\times}3{\times}2$ factorial arrangement in a completely randomized design using two restraint methods (shackle and cone), three durations of restraint (10, 30, and 60 s), and two categories of live body weight ($1.8{\pm}0.1kg$ as lightweight and $2.8{\pm}0.1kg$ as heavyweight). Results: Irrespective of the duration of restraint and body weight, the coned chickens were found to have lower plasma corticosterone (p<0.01), lactate (p<0.001), lower meat drip loss (p<0.01), cooking loss (p<0.05), and higher blood loss (p<0.05) compared with their shackled counterparts. The duration of restraint had significant effects on the meat initial pH (p<0.05), ultimate pH (p<0.05), and yellowness (p<0.01). The lightweight broilers exhibited higher (p<0.001) blood loss and lower (p<0.05) cooking loss compared to the heavyweight broilers, regardless of the restraint method used and the duration of restraint. However, the interaction between the restraint method, duration of restraint, and body weight contributed to differences in pre-slaughter stress and meat quality. Therefore, the interaction between the restraint method and the duration of restraint affected the meat shear force, lightness ($L^*$) and redness ($a^*$). Conclusion: The duration of restraint and body weight undoubtedly affect stress responses and meat quality of broiler chickens. Regardless of the duration of restraint and body weight, the cone restraint resulted in notably lower stress, lower meat water loss, and higher blood loss compared to shackling. Overall, the findings of this study showed that restraint method, duration of restraint, and body weight may affect the stress response and meat quality parameters in broilers and should be considered independently or interactively in future studies.

Probability-based Critical Path Estimation for PERT Networks of Repetitive Activities (반복작업 PERT 네트워크의 확률기반 주공정 산정기법)

  • Yi, Kyoo-Jin
    • Journal of the Korea Institute of Building Construction
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    • v.18 no.6
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    • pp.595-602
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    • 2018
  • Network-based scheduling methods can be classified into CPM method and PERT method. In the network scheduling chart, critical path can be estimated by performing the forward calculation and the backward calculation though the paths in the network chart. In PERT method, however, it is unreasonable to simply estimate the critical path by adding the sum of the activity durations in a specific path, since it does not incorporate probabilistic concept of PERT. The critical path of a PERT network can change according to the target period and deviation, and in some cases, the expected time of the critical path may not be the path with longest expected time. Based on this concept, this study proposes a technique to derive the most-likely critical path by comparing the sum of estimated time with the target time. It also proposes a method of systematically deriving all alternate paths for a network of repetitive activities. Case studies demonstrated that the most-likely critical path is not a fixed path and may vary according to the target period and standard deviation. It is expected that the proposed method of project duration forecasting will be useful in construction environment with varying target date situations.