• 제목/요약/키워드: dual culture

검색결과 264건 처리시간 0.024초

시기심과 고통: 자기계발 서사에 나타난 감정 연구 - 막장드라마 <아내의 유혹>을 중심으로 - (The Envy and Suffering: Feelings of Characters Appearing in Self-Development Narrative - Focusing on a Soap Opera)

  • 박숙자
    • 비교문화연구
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    • 제46권
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    • pp.21-42
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    • 2017
  • 이 연구에서는 신자유주의 시대의 감정 양상을 막장드라마(Soap Opera)를 통해 살펴보고자 하였다. 특히 '시기심(envy)'에 초점을 맞춰 분석하였다. 시기심은 비슷한 처지에 있는 사람들 사이에서 나타나는 비교의 감정으로 질투심과 달리 이자관계(dual relationship) 안에서 나타나는데, 상대방의 행운(승리)을 불쾌, 부인, 방해하려는 역동을 가지고 있다. 시기심은 이처럼 부정적으로 감정으로 간주되는 게 일반적인데, 막장드라마 속에서 재현되는 시기심은 몫이 한정된 경쟁에 참여하는 주체들의 열정으로 전치되어 나타나고 있다. '시기심'으로 촉발된 경쟁이 결국 자기계발의 성공 드라마로 재현되는 양상이 그것이다. 그런데 이렇게 재현된 시기심은 무한경쟁에 참여하는 주체들의 열정으로 비화되거나 혹은 사악한 행동으로 개별화되면서 인물이 놓인 구조적 맥락이나 그 속에서 발원하는 고통의 문제를 외면하고 있다. <아내의 유혹>은 인물의 감정을 '시기심'으로 형상화함으로써 신자유주의적 주체를 재현하는 것 뿐만 아니라 인물이 처한 고통이나 맥락을 변용시켜 냄으로써 탈법적이고 비윤리적 현실논리를 승인할 가능성이 높다는 점에서 기존의 통속극과 궤를 달리해서 봐야 한다.

남성의 시장노동과 가사노동 - 신문기사 내용에 대한 질적 분석을 중심으로 (Men's Participation of the Market Work and the Housework - Focused on the Qualitative Analysis of the Contents in the Newspaper Articles)

  • 조성은;정지영;윤소영
    • 가정과삶의질연구
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    • 제24권1호
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    • pp.129-140
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    • 2006
  • The purpose of this study was to understand the labor division between men's housework and market work and to research the change of men's contribution to housework. And it was also examined what kinds of valuables work on a change in men's market work and housework. The information about men's change in the labor division was collected from the biggest 5 news papers in Korea for last five years for this study. And either men's new participation trend to household work did. Words searched for this study were men, labor, family, home, housewife, work, household work, father etc. Korean men have experienced a overloaded breadwinner role and have worked most in the world since 1960s. But Korean men's working time was continuously decreased a little every year. As Korean Companies had fired many worker during IMP economic crisis period, Korean men had to work more than before because of decreased fellows and they should have concentrated on their work at the sacrifice of private life and family-sharing time. On the other hand, some men were started to participate to do housework as a results of long-unemployment and early retirement after this periods. 5 day working system be in forced gradually since 2003 especially make men come back home and interest on housework. So Korean men's housework participation is gradually increasing by the practical application of sex-equitable politics such as 5 day working system and men's suspension regime for baby care. father's increased participation to children education and care, men's new family-oriented life style, dual-sexuality education system, and socially changed perception to husband housemaker. These interrelated trends demands us to shape a new labor division pattern in the family that make change the breadwinner/homemaker conception by the gender role. Now, all of family, men, women, and children have to join housework. It would help women, men, and all families make more human and equitable relationship.

김치로부터 항진균 활성 Lactobacillus plantarum의 분리 및 특성 규명 (Antifungal Activity of Lactobacillus plantarum Isolated from Kimchi)

  • 양은주;장해춘
    • 한국미생물·생명공학회지
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    • 제36권4호
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    • pp.276-284
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    • 2008
  • 숙성된 김치로부터 항진균 활성을 나타내는 균주 1종과 감수성을 나타내는 곰팡이 1종을 분리하였다. 분리된 균주는 형태학적, 생화학적 특성 조사와 165 rRNA 염기서열 결정을 통한 균주 동정 결과 Lactobacillus plantarum AF1으로 명명하였고, 감수성 곰팡이는 ITS-5.8S rRNA 염기서열 분석을 통하여 Epicoccum nigrum KF-1으로 명명하였다. Dual culture overlay assay를 통한 Lb. plantarum AF1의 항진균 활성 실험 결과 A. ochraceus, A fumigatus, C. gossypiicola 등 식품 부패 곰팡이 및 병원성 곰팡이에 강한 생육 저해 활성을 나타내었다. 또한 항균 물질에 의한 항미생물 활성 범위를 측정한 결과 항진균 활성 외에도 식중독균주를 포함한 그람 양성 및 음성 세균들에 강한 저해 활성을 나타내어 Lb. plantarum AF1은 넓은 항미생물 활성 범위를 가지는 것을 알 수 있었다. Lb. plantarum AF1의 생육에 따른 항진균 활성을 측정한 결과 항진균 활성은 배양 20시간부터 최대 활성(3,200 AU/ml)을 나타내어 120시간까지 활성이 감소되지 않고 유지되었다. 항진균 물질의 안정성 실험을 통하여 AF1 항진균 물질은 산성의 pH(pH $3.0{\sim}4.0$)와 열에 안정한 물질이며, 단백분해효소 처리에 영향을 받지 않으므로 비단백질성 물질이거나 단백분해효소의 영향을 받지않는 구조의 물질임을 추정하였다. AF1 항진균 물질의 분자량을 예측하기 위하여 Lb. plantarum AF1의 배양액을 3,000 Da 이상과 이하의 분획으로 나누어 항진균 활성을 측정한 결과 AF1 항진균 물질은 분자량 3,000 Da 미만의 물질임을 확인하였다. 본 실험에서 분리한 김치유산균인 Lb. plantarum AF1은 넓은 범위의 항진균 활성 및 항세균 활성을 나타내므로 강력한 천연 식품보존제 및 사료보존제로서 활용이 기대된다.

배지최적화를 통한 재조합 바실러스 서브틸리스에서 바실러스 아밀로리퀴파시엔스 유래 γ-글루타밀펩타이드전달효소의 대량생산 (Overproduction of a γ-glutamyltranspeptidase from Bacillus amyloliquefaciens in Bacillus subtilis through medium optimization)

  • 조혜빈;제텐드라 쿠마르 로이;박우진;전병운;김영완
    • 한국식품과학회지
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    • 제49권6호
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    • pp.610-616
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    • 2017
  • 본 연구를 통해 BAGGT를 재조합 B. subtilis를 이용하여 대량 생산하기 위하여 유전자 클로닝, 발현 시스템 구축 및 배지 최적화를 진행하였다. 이중프로모터 시스템을 이용하여 야생형 균주에 비해 42배 효소 생산성이 향상된 발현 시스템을 구축하였다. 또한 PBD 분석을 통해 당밀과 CSL이 재조합 B. subtilis 시스템에서 BAGGT의 생산성에 큰 영향을 주는 인자임을 확인하였으며, 염류의 첨가에 의한 효소 생산성 증대 효과는 미비하거나 부정적이었다. 탄소원으로 당밀을 선택하고 고가의 질소원인 트립톤을 저가의 CSL로 교체한 후 CCD 분석을 통해서 결정된 최적배지 사용 시 최적화 이전의 LB 배지 대비 4.3배의 생산성 증대를 이루었으며, 이는 LB 배지에서 야생형 균주의 BAGGT 생산성 대비 180배의 효소 생산성 개선에 해당하였다. 본 연구를 통해 식품용 효소로서 BAGGT의 대량생산을 위한 공정을 구축하였으며, 이후 정미성 소재 생산에 활용할 수 있을 것으로 기대한다.

미술과 집단성 (Art and Collectivity)

  • 곽건초
    • 미술이론과 현장
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    • 제4호
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    • pp.181-202
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    • 2006
  • "When it comes to art, nationalism is a goodticket to ride with", says the title of a report in the Indian Express (Mumbai, 29 Oct 2000). The newspaper report goes on to say that since Indian art was kept "ethnic" by colonialism, national liberation meant opening up to the world on India's own terms. Advocacy, at the tail end of the 20th century, would contrast dramatically with the call by Rabindranath Tagore, the founder of the academy at Santiniketan in 1901, to guard against the fetish of nationalism. "The colourless vagueness of cosmopolitanism," Tagore pronounced, "nor thefierce self-idolatry of nation-worship, is the goal of human history" (Nationalism, 1917). This contrast is significant on two counts. First is the positive aspect of "nation" as a frame in art production or circulation, at the current point of globalization when massive expansion of cultural consumers may be realized through prevailing communication networks and technology. The organization of the information market, most vividly demonstrated through the recent FIFA World Cup when one out of every five living human beings on earth watched the finals, is predicated on nations as categories. An extension of the Indian Express argument would be that tagging of artworks along the category of nation would help ensure greatest reception, and would in turn open up the reified category of "art," so as to consider new impetus from aesthetic traditions from all parts of the world many of which hereto fore regarded as "ethnic," so as to liberate art from any hegemony of "international standards." Secondly, the critique of nationalism points to a transnational civic sphere, be it Tagore's notion of people-not-nation, or the much mo re recent "transnational constellation" of Jurgen Habermas (2001), a vision for the European Union w here civil sphere beyond confines of nation opens up new possibilities, and may serve as a model for a liberated sphere on global scale. There are other levels of collectivity which art may address, for instance the Indonesian example of local communities headed by Ketua Rukun Tetangga, the neighbourhood headmen, in which community matters of culture and the arts are organically woven into the communal fabric. Art and collectivity at the national-transnational level yield a contrasting situation of, on the idealized end, the dual inputs of local culture and tradition through "nation" as necessary frame, and the concurrent development of a transnational, culturally and aesthetically vibrant civic sphere that will ensure a cosmopolitanism that is not a "colourless vagueness." In art historical studies, this is seen, for instance, in the recent discussion on "cosmopolitan modernisms." Conversely, we may see a dual tyranny of a nationalism that is a closure (sometimes stated as "ethno-nationalism" which is disputable), and an internationalism that is evolved through restrictive understanding of historical development within privileged expressions. In art historical terms, where there is a lack of investigation into the reality of multiple modernisms, the possibility of a democratic cosmopolitanism in art is severely curtailed. The advocacy of a liberal cosmopolitanism without a democratic foundation returns art to dominance of historical privileged category. A local community with lack of transnational inputs may sometimes place emphasis on neo-traditionalism which is also a double edged sword, as re kindling with traditions is both liberating and restrictive, which in turn interplays with the push and pull of the collective matrix.

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폐결핵 또는 기타 질환환자에 있어서의 폐진균증에 관한 연구 (Pulmonary Fungal Infection in Patients with Healed Tuberculosis or Other Underlying Diseases)

  • Kim Sang Jae;Hong Young Pyo;Kim Sung Chin
    • 미생물학회지
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    • 제19권3호
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    • pp.142-152
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    • 1981
  • One hundred and thirteen healed pulmonary tuberculosis patients and 11 patients with other underlying diseases were studied for evidence of pulmonary fungal infection because of persisting hemoptysis or chronic cough. Rediological, mycological and serological investigations revealed that 54 out of 124 patients were evidently infected with one or more species of fungi. A. fumigatus was isolated from 4 out of 70 patients whose sera did not react with antigens from this fungus, while it was isolated from 43 out of 47 serological reactors to this fungus. Chest radiography showed a distinct fungus ball in a cyst of one patient and in a preformed cavity in the lung of 17 healed tuberculosis patients and two other patients. The latter two patients were infected with A.flavus. Two patients, who were under the long period of immunosuppressive therapy, apparently succumbed to invasive aspergillosia due to A.fumigatus. A single or dual infection with A. flavus, A. nidulans, A.nidulans var. latus, C. albicans, and P. boydii were noticed in some patients without mycetomal shadow on chest radiographs. Young mycelial extract (ME) of A.fumigatus detected antibody in 95.8 percent of the sera from patients infected with this fungus, while it was isolated from 43 out of 47 serological reactors to this fungus. Chest radiography showed a distinct fungus ball in a cyst of one patient and in a performed cavity in the lung of 17 healed tuberculosis patients and two other patients. The latter two patients were infected with A. flavus. Two patients, who were under the long period of immunosuppressive therapy, apparently succumbed to invasive aspergillosis due to A.fumigatus. A single or dual infection with A. flavus, A. nidulans, A. niduans var. latus, C. albicans, and P. boydii were noticed in some patients without mycetomal shadow on chest radiographs. Young mycelial extract (ME) of A.fumigatus detected antibody in 95.8 percent of the sera from patients infected with this fungus, while the commercial culture filtrate antigen (GL) yielded 78.7 per cent positive result. Culture filtrate antigen, however, was comparable with ME. There was no single antigen with which all the serum specimens reacted. Fractionation of ME resulted in a loss of some activity although it excluded substances that reacted with C-reactive protein in a loss of some activity although it excluded substances that reacted with C-reactive protein. Most reactive and specific precipitinogens distributed in the fraction (FB) which was precipitable at 75 percent saturation with ammonium sulfate and eluted in a second peak in order from gel-filtration and which contained mostly proteinic components. Glycoproteins or polysaccharides rich fractions (FA and ASI) were relatively less effective in detecting antibody. Demonstration of antibody in the serum from patients using a battery of fungal antigens and of etiologically related fungi from clinical specimens are very useful laboratory procedures for the diagnosis of pulmonary fungal infection which is a common complication of tuberculosis.

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The culture conditions for mycelial growth and sclerotial formation of Polyporus umbellatus

  • Lee, Min Woong;Chang, Kwang Chun;Shin, Do Bin;Lee, Kyung Rim;Im, Kyung Hoan;Jin, Ga-Heon;Shin, Pyung Gyun;Xing, Yong Mei;Chen, Juan;Guo, Shun Xing;Lee, Tae Soo
    • 한국버섯학회지
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    • 제11권4호
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    • pp.194-200
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    • 2013
  • Polyporus umbellatus (Syn. Grifola umbellata) is a sclerotium forming mushroom belongs to family Polyporaceae of Polyphorales, Basidiomycota. The sclerotia of P. umbellatus have long been used for traditional medicines in China, Korea and Japan. This study was initiated to obtain the basic data for artificial sclerotial production of P. umbellatus. Here, we investigated the favorable conditions for mycelial growth of P. umbellatus and its symbiotic fungus Armillaria mellea. We also evaluate the favorable carbon and nitrogen sources for sclerotial formation in dual culture between P. umbellatus and A. mellea. The favorable conditions for mycelial growth of P. umbellatus were $20^{\circ}C$ and pH 4, while optimal conditions for mycelial growth of A. mellea were $25^{\circ}C$ and pH 6. The carbon sources for optimal mycelial growth of P. umbellatus were fructose and glucose, while carbon sources for favorable mycelial growth of A. mellea were also fructose and glucose. The nitrogen sources for favorable mycelial growth P. umbellatus were peptone and yeast extract, while optimal mycelial growth of A. mellea were obtained in peptone and yeast extract. When P. umbellatus and A. mellea were dual cultured on carbon sources, sclerotia were induced on basal media supplemented with glucose, fructose and maltose at pH 4~6, while nitrogen sources inducing sclerotia were basal media supplemented with peptone and yeast extract for 60 days at $20^{\circ}C$ under dark condition.

이중점화기법을 통해 본 남녀 대학생의 '성공/실패'에 대한 암묵적 표상 (A Study of College students' implicit representations of 'success/failure' by dual-priming task)

  • 조혜자 ;방희정 ;조숙자 ;김현정
    • 한국심리학회지 : 문화 및 사회문제
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    • 제14권1호
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    • pp.101-123
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    • 2008
  • 본 연구는 남녀 대학생을 대상으로, 어머니와 연합된 성공/실패에 대한 표상을 알아보기 위해 시도되었다. 이를 위해 연구 1에서는 어머니/바구니와 성공/실패를 이중점화자극으로 100ms 동안 제시하고 난 뒤, 150ms 후(SOA 250ms)에 수용/거부와 관련된 목표자극(probe)을 제시하고 어의판단에 걸리는 시간을 측정하였다. 그 결과, 어머니조건의 반응이 통제조건보다 빨랐으며, 수용에 대한 반응이 거부 반응보다 빨랐고, 여자집단의 반응이 남자보다 빨랐다. 연구 2에서는 남녀별로 애착 고집단과 저집단을 나누어, 어머니와 성공/실패를 이중점화시킨 뒤 수용/거부에 대한 어의판단 시간을 측정하였다. 그 결과, 성공/실패와 수용/거부 간에 상호작용이 나타나, 성공-수용에 대한 반응은 가장 빨랐고, 실패-수용, 실패-거부, 성공-거부의 순으로 반응이 느려졌다. 한편 남자집단에서는 애착고저 집단 간에 유의미한 차이가 없고 상호작용도 나타나지 않았지만, 여자집단에서는 3원 상호작용이 나타났다. 즉 여자 애착 고집단에서는 성공-수용, 성공-거부에 대한 반응차이가 크지 않았고, 실패-거부에 대한 반응이 매우 느렸으나, 여자 애착 저집단에서는 성공-수용은 매우 빠른데 비해 성공-거부는 매우 느렸다. 이러한 결과는 자기 긍정성 및 성별, 애착유형에 따라 다른 성공/실패 도식과 관련하여 논의되었다.

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요양보호사의 교육, 서비스 질, 전문성, 긍정심리자본의 관계 연구 (A study on the of professionalism and positive psychological capital on the effect of elderly care worker education on service quality)

  • 김봉화;고윤순
    • 문화기술의 융합
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    • 제10권3호
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    • pp.613-623
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    • 2024
  • 본 연구는 요양보호사 교육이 서비스 질에 미치는 영향에 있어 전문성과 긍정심리자본의 이중매개효과를 분석함으로써 요양보호사 교육의 지향점을 탐색하고자 수행하였다. 분석 결과 요양보호사 교육은 서비스 질, 전문성, 긍정심리자본에 유의한 영향을 미치는 것으로 나타나 요양보호사 교육에 대한 인식이 긍정적일수록 서비스 질, 전문성, 긍정심리자본에 대한 인식도 긍정적으로 높아질 수 있음을 확인하였다. 또한 전문성과 긍정심리자본은 각각 요양보호사 교육이 서비스 질에 미치는 영향을 매개하는 것으로 나타남으로서 요양보호사 교육은 전문성을 향상시킴으로써서비스 질을 높일 수 있고 요양보호사 교육은 긍정심리자본을 향상시킴으로써 서비스 질을 높일 수 있는 요인임을알 수 있다. 전문성과 긍정심리자본은 요양보호사 교육이 서비스 질에 미치는 영향을 이중매개함으로서 전문성이 긍정심리자본에 유의한 영향을 미치고 있음을 알 수 있었다. 이에 따라 요양보호사 교육은 전문성을 향상시키고, 향상된 전문성은 긍정심리자본을 증진시키며, 이를 통해 서비스 질이 높아진다는 것을 확인하였다. 본 연구결과에 따라요양보호사 교육에 있어 전문성의 함양이 중요하며, 전문성의 함양을 통한 긍정심리자본의 증진이 서비스 질을 높일수 있다는 것을 실증적으로 확인할 수 있었다. 이에 따라 향후 진행될 요양보호사 교육에 전문성과 긍정심리자본의 향상과 관련된 교육이 포함되어야 한다는 것을 조망할 수 있었다는 데에 의의가 있다.

Antimicrobial Active Substances from Entomopathogenic Fungi (Various Applications of Entomopathogenic Fungi)

  • Shin, Tae Young;Woo, Soo Dong;Kim, Jeong Jun
    • 한국균학회소식:학술대회논문집
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    • 한국균학회 2016년도 춘계학술대회 및 임시총회
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    • pp.13-13
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    • 2016
  • Insects constitute the largest and most diverse group of animals in the world. They also serve as the hosts or nutrient sources for an immense assemblage of pathogens, parasites, and predators. More than 700 fungal species from 100 genera have adopted an entomopathogenic lifestyle. Although entomopathogenic fungi were studied as only biocontrol agents against a variety of pests in various countries, it has been recently focused their additional roles in nature. They are antagonists to/against plant pathogens, endophytes, and possibly even plant growth promoting agents. The potential antimicrobial effect against fungal plant pathogens by an isolate of entomopathogenic fungi including Beauveria bassiana, Lecanicillium spp., and Isaria fumosorosea have been reported since late 1990s, but wasn't reported pathogenicity of the isolate against pests. Later, a Canadian Lecanicillium sp. isolate and L. longisporium isolated from Vertalec$^{(R)}$ showed simultaneous control effect against both aphid and cucumber powder mildew. Therefore, the antimicrobial activities of 342 fungi isolates collected from various regions and conditions in Korea were evaluated against plant pathogenic fungus Botrytis cinerea using dual culture technique on agar plate. As a result, 186 isolates (54.4%) shown the antifungal activity against B. cinerea. The culture filtrates of selected fungi completely suppressed the growth of the microorganisms, indicating that suppression was due to the presence of antimicrobial substances in the culture filtrate. Mode of action of these fungi against insect involves the attachment of conidia to the insect cuticle, followed by germination, cuticle penetration, and internal dissemination throughout the insect. During infection process, secreted enzymes, proteinous toxins, and/or secondary metabolites secreted by entomopathogenic fungi can be used to overcome the host immune system, modify host behavior, and defend host resources. Recently, secondary metabolites isolated from entomopathogenic fungi have been reported as potential bioactive substances. Generally, most of bioactive substances produced by entomopathogenic fungi have reported low molecular weight (lower than 1,000 g/mol) as peptide and, in contrast the high molecular weight fungal bioactive substances are rare. Most substances based on entomopathogenic fungi were shown antimicrobial activity with narrow control ranges. In our study we analyzed the antimicrobial substances having antagonistic effects to B. cinerea. Antimicrobial substances in our fungal culture filtrates showed high thermostability, high stability to proteolytic enzymes, and hydrophilicity and their molecular weights were differed from substance. In conclusion, entomopathogenic fungi showed pathogenicity against insect pests and culture filtrate of the fungi also shown to antimicrobial activity. In the future, we can use the entomopathogenic fungi and its secondary metabolites to control both insect pest control and plant pathogenic fungi simultaneously.

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