Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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The culture conditions for mycelial growth and sclerotial formation of Polyporus umbellatus

  • Lee, Min Woong (Department of Life Science, Dongguk University) ;
  • Chang, Kwang Chun (Department of Life Science, Dongguk University) ;
  • Shin, Do Bin (Division of Life Sciences, Incheon National University) ;
  • Lee, Kyung Rim (Division of Life Sciences, Incheon National University) ;
  • Im, Kyung Hoan (Division of Life Sciences, Incheon National University) ;
  • Jin, Ga-Heon (Department of Ophthalmic Optics, Shinheung University) ;
  • Shin, Pyung Gyun (Mushroom Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Xing, Yong Mei (Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College) ;
  • Chen, Juan (Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College) ;
  • Guo, Shun Xing (Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College) ;
  • Lee, Tae Soo (Division of Life Sciences, Incheon National University)
  • Received : 2013.12.07
  • Accepted : 2013.12.30
  • Published : 2013.12.31
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Abstract

Polyporus umbellatus (Syn. Grifola umbellata) is a sclerotium forming mushroom belongs to family Polyporaceae of Polyphorales, Basidiomycota. The sclerotia of P. umbellatus have long been used for traditional medicines in China, Korea and Japan. This study was initiated to obtain the basic data for artificial sclerotial production of P. umbellatus. Here, we investigated the favorable conditions for mycelial growth of P. umbellatus and its symbiotic fungus Armillaria mellea. We also evaluate the favorable carbon and nitrogen sources for sclerotial formation in dual culture between P. umbellatus and A. mellea. The favorable conditions for mycelial growth of P. umbellatus were $20^{\circ}C$ and pH 4, while optimal conditions for mycelial growth of A. mellea were $25^{\circ}C$ and pH 6. The carbon sources for optimal mycelial growth of P. umbellatus were fructose and glucose, while carbon sources for favorable mycelial growth of A. mellea were also fructose and glucose. The nitrogen sources for favorable mycelial growth P. umbellatus were peptone and yeast extract, while optimal mycelial growth of A. mellea were obtained in peptone and yeast extract. When P. umbellatus and A. mellea were dual cultured on carbon sources, sclerotia were induced on basal media supplemented with glucose, fructose and maltose at pH 4~6, while nitrogen sources inducing sclerotia were basal media supplemented with peptone and yeast extract for 60 days at $20^{\circ}C$ under dark condition.

Keywords

References

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