• Korean Journal of Environmental Agriculture
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• v.11 no.1
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• pp.41-49
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• 1992

To determine the efficacy of uniconzaole[(E)-1-(4-chlorophenyl)-4,4-dimethy 2-(1,2,4-triazol-1-yl)-1-penten-3-ol)](XE-1019) as a phytoprotectant against $O_3$ injury in tomato plants(Lycopersicon esculentum Mill. 'Pink Glory'), plants were given a 50ml soil drench of uniconazole solution at concentrations of 0.001, 0,01, 0.1 and 0.2mg/pot thirteen days prior to $O_3$ fumigation. All four uniconazole concentrations were effective in providing protection against $O_3$ exposure(16h at 0.3 ppm). Uniconazole treatment above 0.001 mg/pot significantly reduced stem elongation, leaf enlargement, leaf area and fresh weight of plant, whereas increased chlorophyll concentration. Transpiration rate on a whole plant basis was reduced by uniconazole treatment and $O_3$ exposure. Uniconazole reduced ethylene production induced by $O_3$ injury but had little or no effect on defoliation of cotyledons and leaf epinasty. Activities of peroxidase (POD) and superoxide dismutase(SOD) were slightly increased by application of uniconazole. With increasing exposure time, $O_3$ increased POD activity but decreased SOD activity. The phytoprotective effects of uniconazole were diminished by applying gibberellin at $10{\sim}20$ ppm. These results suggest that the phytoprotective effects of uniconazole are related to its role of increasing activities of free radical scavengers such as POD and SOD, in addition to growth-retardation as an anti-gibberellin.

• Korean Journal of Environmental Agriculture
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• v.11 no.1
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• pp.50-58
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• 1992

Studies were conducted to determine the combined effect of uniconazole [(E) -1-(4-chlorophenyl)-4, 4-demethyl 2-(1,2,4 triazol-1-yl)-1-penten-3-ol] and silver thiosulfate $[Ag {(S_2O_3)}^3\;_2-]$ (STS) on reduction of ozone injury in tomato plants(Lycopersicon esculentum Mill. 'Pink Glory'). Plants were given a 50ml soil drench of uniconazole at concentrations of 0, 0.001, 0.01 and 0.1 mg/pot at the stage of emerging 4th leaf. Two days prior to ozone fumigation, STS solution contained 0.05% Tween-20 was also sprayed at concentrations of 0, 0.3 and 0.6 mM. Uniconazole at 0.01 mg/pot and STS at 0.6 mM were effective in providing protection against ozone exposure(20h at 0.2ppm) without severe retardation of plant height and chemical phytotoxicity, respectively. Combined treatment with uniconazole, STS significantly reduced ozone injury at the lower concentration than a single treatment with uniconazole or STS. Uniconazole treatment reduced plant height, stem elongation and transpiration rate on a whole plant level and increased chlorophyll concentration. STS did not give any effect on plant growth and chlorophyll content but increased transpiration rate in non-ozone-fumigated plants. Ethylene production in the leaves of ozone-fumigated plants was decreased by uniconazole and STS pretreatment, but there was no protective effect on epinasty of leaves in uniconazole-treated plants. STS increased ethylene production in non-ozone-fumigated plants, but it significantly reduced the degree of epinasty and defoliation of cotyledons when plants were exposed to ozone. Uniconazole slightly increased superoxide dismutase and peroxidase activities. But STS showed little or no effects on such free radical scavengers. Day of flowering after seeding was shortened and percentages of fruit set were increased by uniconazole treatment. STS was highly effective on protecting reduction of fruit set resulting from ozone fumigation. These results suggest that combined use of uniconazole and STS should provide miximum protection against ozone injury without growth retardation resulting in yield loss.

• The Plant Pathology Journal
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• v.29 no.4
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• pp.386-396
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• 2013

Reactive oxygen species (ROS) generation in tomato plants by Ralstonia solanacearum infection and the role of hydrogen peroxide ($H_2O_2$) and nitric oxide in tomato bacterial wilt control were demonstrated. During disease development of tomato bacterial wilt, accumulation of superoxide anion ($O_2{^-}$) and $H_2O_2$ was observed and lipid peroxidation also occurred in the tomato leaf tissues. High doses of $H_2O_2$ and sodium nitroprusside (SNP) nitric oxide donor showed phytotoxicity to detached tomato leaves 1 day after petiole feeding showing reduced fresh weight. Both $H_2O_2$ and SNP have in vitro antibacterial activities against R. solanacearum in a dose-dependent manner, as well as plant protection in detached tomato leaves against bacterial wilt by $10^6$ and $10^7$ cfu/ml of R. solanacearum. $H_2O_2$- and SNP-mediated protection was also evaluated in pots using soil-drench treatment with the bacterial inoculation, and relative 'area under the disease progressive curve (AUDPC)' was calculated to compare disease protection by $H_2O_2$ and/or SNP with untreated control. Neither $H_2O_2$ nor SNP protect the tomato seedlings from the bacterial wilt, but $H_2O_2$ + SNP mixture significantly decreased disease severity with reduced relative AUDPC. These results suggest that $H_2O_2$ and SNP could be used together to control bacterial wilt in tomato plants as bactericidal agents.

• Korean journal of applied entomology
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• v.56 no.4
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• pp.351-356
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• 2017

For determining the insecticidal effect of Carbofuran on the Brown planthopper, Nilaparvata lugens, sucking toxicity by drenching application, sucking and contact toxicity by leaf dipping application, and contact toxicity by topical application were examined. Drenching caused two types of mortality patterns. One was logarithmic curve at a relatively high concentration (8~30 ppm) with over 40% mortality in 24 h, and the other was an S-shaped curve at low concentrations (1~4 ppm) with over 60% mortality on the fifth day after Carbofuran treatment. Leaf dipping application caused a rapid increase in mortality in a day, and this effect decreased steadily with time. Topical application showed steep increase in mortality in a day, and hardly increased thereafter. The best mortality evaluation time for the drenching application was the second day (42 h), and that for the leaf dipping and topical applications was the first or second day after Carbofuran application. When the insecticide has systemic effects, drench application provides the best efficacy and its insecticidal effects persist for a longer time than any other application method.

• FLOWER RESEARCH JOURNAL
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• v.19 no.3
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• pp.127-138
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• 2011

Four different plant growth retardants (PGRs), paclobutrazol, flurprimidol, daminozide, and chlormequat, were applied to potted Saxifraga rosacea 'Kumoma' and 'Kumoma-Gusa' plants for control of the growth and flowering. Paclobutrazol (10, 20, 40, $80mg{\cdot}L^{-1}$), flurprimidol (5, 10, 20, $40mg{\cdot}L^{-1}$), daminozide (500, 1000, 2000, $4000mg{\cdot}L^{-1}$), and chlormequat (50, 100, 200, $400mg{\cdot}L^{-1}$) were applied to the plants by a foliar spray or drenching. In 'Kumoma', application of $40mg{\cdot}L^{-1}$ paclobutrazol by a foliar spray or drenching reduced plant height by 12.5 and 12.6 cm, and flower length by 3.4 and 3.3 cm, respectively. On the other hand, in 'Kumoma-Gusa', drenching of paclobutrazol reduced plant height by 10.7 to 12.6 cm and flower length by 2.0 to 3.9 cm with increasing concentration, but the number of florets almost fell to 20 as compared to 40.5 in the control. 'Kumoma-Gusa' plants drenched with $80mg{\cdot}L^{-1}$ paclobutrazol and sprayed with $40mg{\cdot}L^{-1}$ flurprimidol had the shortest heights of 10.7 and 9.9 cm, and floral length of 2.0 and 1.5 cm, respectively. A flurprimidol drenching at $40mg{\cdot}L^{-1}$ delayed the harvest by 3-13 days as compared to the control and the smallest number of florets, 15.6, was observed in this treatment. In both cultivars, chlormequat and daminozide did not effectively influence the growth and flowering. However, number of florets increased to more than 41 at all concentrations and up to 63, the greatest floret number, with chlormequat drench in 'Kumoma-Gusa'. These results demonstrated that over $40mg{\cdot}L^{-1}$ of paclobutrazol or 5 to $20mg{\cdot}L^{-1}$ of flurprimidol could be used as PGRs to control the growth of floral length and flowering for improving potted plant quality in S. rosacea 'Kumoma' and 'Kumoma-Gusa'.