• Title/Summary/Keyword: direct transformation

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High-frequency plant regeneration from leaf-disc cultures of Jatropha curcas L.: an important biodiesel plant

  • Deore, Ajay C.;Johnson, T. Sudhakar
    • Plant Biotechnology Reports
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    • v.2 no.1
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    • pp.7-11
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    • 2008
  • A simple, high-frequency and reproducible protocol for induction of adventitious shoot buds and plant regeneration from leaf-disc cultures of Jatropha curcas L. has been developed. Adventitious shoot buds were induced from very young leaf explants of in vitro germinated seedlings as well as mature field-grown plants cultured on Murashige and Skoog's (MS) medium supplemented with thidiazuron (TDZ) ($2.27{\mu}M$), 6-benzylaminopurine (BA) ($2.22{\mu}M$) and indole-3-butyric acid (IBA) ($0.49{\mu}M$). The presence of TDZ in the induction medium has greater influence on the induction of adventitious shoot buds, whereas BA in the absence of TDZ promoted callus induction rather than shoot buds. Induced shoot buds were multiplied and elongated into shoots following transfer to the MS medium supplemented with BA ($4.44{\mu}M$), kinetin (Kn) ($2.33{\mu}M$), indole-3-acetic acid (IAA) ($1.43{\mu}M$), and gibberellic acid ($GA_3$) ($0.72{\mu}M$). Well-developed shoots were rooted on MS medium supplemented with IBA ($0.5{\mu}M$) after 30 days. Regenerated plants after 2 months of acclimatization were successfully transferred to the field without visible morphological variation. This protocol might find use in mass production of true-to-type plants and in production of transgenic plants through Agrobacterium/biolistic-mediated transformation.

RFM for High Resolution Satellite Sensor Modeling (RFM을 이용한 고해상도 인공위성 센서모델링)

  • 조우석;이동구
    • Korean Journal of Remote Sensing
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    • v.18 no.6
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    • pp.337-344
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    • 2002
  • In general, in order to obtain position information from satellite images, satellite sensor model which represents the geometric relationship between sensor and targeted area should be established in the first place. However, it is not simple for modelling pushbroom satellite sensor due to the image capturing process. In recent development of new generation imaging sensors, a generic sensor model, which is applicable to all types of sensors such as frame, pushbroom, whiskbroom, and SAR is in great need to the remote sensing and photogrammetry community. In this paper, the RFM as sensor model was implemented with KOMPSAT EOC and SPOT satellite images and analyzed in cases where the number and distribution of ground control points were varied. The test results of RFM were presented and compared with those of Direct Linear Transformation(DLT).

Flipped Learning: Strategies and Technologies in Higher Education

  • Miziuk, Viktoriia;Berdo, Rimma;Derkach, Larysa;Kanibolotska, Olha;Stadnii, Alla
    • International Journal of Computer Science & Network Security
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    • v.21 no.7
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    • pp.63-69
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    • 2021
  • Flipped learning is necessary for modern education but quite difficult to implement. In pedagogical science, the question remains to what extent the practical work of the teacher in combination with the technologies of flipped learning will improve the quality of higher education. The aim of this article is to study the effectiveness and feasibility of using flipped learning technologies, assessing their perception by students (advantages and problems), identified an algorithm for introducing flipped learning technology in higher education institutions. Research methods. The main method is an experiment. An evaluation of the effectiveness of the study was conducted using a questionnaire and observation method. Statistical methods were used to evaluate the results of the experiment. The research hypothesis is that flipped learning allows the teacher to spend more time on an individual approach, to understand the real needs of students, and provide effective feedback, thereby improving the quality of learning and motivation of students, especially while studying complex material. The results of the study are to prove the effectiveness of the technology of flipped education in the study of complex disciplines, courses, topics. The use of flipped learning strategies improves the self-regulation of the educational process, group work skills, improves students' ability to learn, overcome difficulties. The technology of flipped learning in the presence of modern technical means and constant work on improving the level of digital literacy is an effective means for students to master complex topics and problematic issues that require additional consideration and discussion. The perspective of further research is the consideration of integrated approaches to the application of flipped learning technologies to the principles of STEAM-education, multilingual and multicultural programs, etc. It is also worth continuing to develop a set of methods aimed at enhancing the student's learning activities, the formation of group work skills, direct participation in creating the foundations of higher education.

The Interpretation of "The Great Learning" within the Korean New Religion Daesoon Jinrihoe (韓國大巡真理會對 《大學》 思想的解釋與轉化)

  • Chung, Yunying
    • Journal of the Daesoon Academy of Sciences
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    • v.34
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    • pp.141-169
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    • 2020
  • This study focuses on the interpretation and transformation of "The Great Learning" within the Korean new religion, Daesoon Jinrihoe. Joseon Dynasty Korea was a member of the Chinese Character Cultural Sphere in East Asia. The examination and recruitment system of the Yuan Dynasty influenced the Joseon Dynasty for a long historical period. Zhu Xi's (朱熹) version of The Four Books were accepted and applied in imperial examinations during the Joseon Dynasty. The 18th century Confucian thinker, Jeong Yak-Yong (丁若鏞), overturned and rebuilt his own system for studying and interpreting The Four Books (四書學). Zhu Xi and Jeong Yak-Yong's systems of thought influenced Confucianism knowledge in that era. The historical figure deified as the Supreme God by Daesoon Jinrihoe, Kang Jeungsan (姜甑山), was trained in the study of The Four Books within that cultural and philosophical context, and this is especially evident in his interpretation and transmission of "The Great Learning." Kang Jeungsan regarding The Great Learning as deeply important. That text combined Confucian discourse on Principle, Mind, and Practice. In his interpretation, The Great Learning was also a medical and religious book that had holy and mysterious powers. In Mugeuk-do and Taegeuk-do (direct predecessors to Daesoon Jinrihoe), Jo Jeongsan interpreted the concept of Sincerity and Regularizing the Mind and incorporated them into doctrine as 'Sincerity, Respectfulness, and Faithfulness' and 'Guarding against Self-deception.' Park Wudang practiced and spread those doctrines to Korea, and Daesoon Jinrihoe devotees continue to follow those doctrines in present times.

Design of Optimal Thermal Structure for DUT Shell using Fluid Analysis (유동해석을 활용한 DUT Shell의 최적 방열구조 설계)

  • Jeong-Gu Lee;Byung-jin Jin;Yong-Hyeon Kim;Young-Chul Bae
    • The Journal of the Korea institute of electronic communication sciences
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    • v.18 no.4
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    • pp.641-648
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    • 2023
  • Recently, the rapid growth of artificial intelligence among the 4th industrial revolution has progressed based on the performance improvement of semiconductor, and circuit integration. According to transistors, which help operation of internal electronic devices and equipment that have been progressed to be more complicated and miniaturized, the control of heat generation and improvement of heat dissipation efficiency have emerged as new performance indicators. The DUT(Device Under Test) Shell is equipment which detects malfunction transistor by evaluating the durability of transistor through heat dissipation in a state where the power is cut off at an arbitrary heating point applying the rating current to inspect the transistor. Since the DUT shell can test more transistor at the same time according to the heat dissipation structure inside the equipment, the heat dissipation efficiency has a direct relationship with the malfunction transistor detection efficiency. Thus, in this paper, we propose various method for PCB configuration structure to optimize heat dissipation of DUT shell and we also propose various transformation and thermal analysis of optimal DUT shell using computational fluid dynamics.

A Study on the Ray Based Broad Band Modeling for Shallow Water Acoustic Wave Propagations (천해 음파전달 모의에 적합한 음선기반 광대역 신호 모델링 기법에 관한 연구)

  • Park Cheol-Soo;Cho Yong-Jin;Ahn Jong-Woo;Seong Woo-Jae
    • The Journal of the Acoustical Society of Korea
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    • v.25 no.6
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    • pp.298-304
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    • 2006
  • This paper proposes a ray-based forward modeling scheme which is suitable for the shallow water acoustic wave propagation simulations. The proposed model comprises of ray tracings for the layered media of which sound speed profiles are interpolated linearly. considerations of plane and spherical wave reflection coefficients. and calculations of the phases and the amplitudes of eigen rays. The main characteristic of the scheme is fast simulation time due to direct calculation of the broad-band time signals in the time-domain, i.e. without transformation of the frequency-domain solutions to the time si 밍 131s. Finally, we applied the model to 4-types of test environments and compared the resulting signals with those of ORCA and Ram in order to validate the proposed model.

AC and DC anodization on the electrochemical properties of SS304L: A comparison

  • Nur S. Azmi;Mohd N. Derman;Zuraidawani Che Daud
    • Advances in materials Research
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    • v.13 no.3
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    • pp.153-160
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    • 2024
  • This study investigates the application of alternating current (AC) and direct current (DC) anodization techniques on stainless steel 304L (SS304L) in an ethylene glycol and ammonium fluoride (NH4F) electrolyte solution to produce a nano-porous oxide layer. With limited research on AC anodizing of stainless steel, this study focuses on comparing AC and DC anodization in terms of current density versus time response, phase analysis using X-ray diffraction (XRD), and corrosion rate determined by linear polarization. Both AC and DC anodization were performed for 60 minutes at 50 V in an electrolyte solution containing 0.5% NH4F and 3% H2O in ethylene glycol. The results show that AC anodization exhibited higher current density compared to DC anodization. XRD analysis revealed the presence of ferrite (α-Fe) and austenite (γ-Fe) phases in the as-received specimen, while both AC and DC anodized specimens exhibited only the γ-Fe phase. The corrosion rate of the AC-anodized specimen was measured at 0.00083 mm/year, lower than the corrosion rate of the DC-anodized specimen at 0.00197 mm/year. These findings indicate that AC anodization on stainless steel offers advantages in terms of higher current density, phase transformation, and lower corrosion rate compared to DC anodization. These results highlight the need for further investigation and exploration of AC anodization as a promising technique for enhancing the electrochemical properties of stainless steel.

Immunological mechanism of Aging : T & B cell changes (면역학적 노화 기전에 관한 연구: T 및 B 세포의 변화)

  • Kim, Jay Sik;Lee, Won Kil;Suh, Jang Soo;Song, Kyung Eun;Lee, Joong Won;Lee, Nan Young;Weksler, Marc E.
    • IMMUNE NETWORK
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    • v.1 no.3
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    • pp.236-243
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    • 2001
  • Background: An immunological approach for aging mechanism appears to be important. Lymphocyte subsets analysis in peripheral blood is widely performed to assess the immune status and to diagnose and monitor various diseases. Some lymphocyte subsets are known to change with age, but only few data about age-related reference ragnes for these subsets in healthy individuals have been reported. So we attempted to report reference ranges for these subsets in each age group and review changes of the results with age for the secondary studies about immune cell function as lymphocyte blast transformation and immunoglobulin gene rearrangement (VDJ) including recombination activating genes (RAG-1 and RAG-2). Methods: Lymphocyte subset analysis was performed on 302 subjects, 189 males and 113 females with age group of all decades of life. Two color direct immunofluorescene flow cytometry (FCM) was done using $Simultest^{TM}$ IMK-Lymphocyte kit (Becton Dickinson, USA), $FACScan^{TM}$ (Becton Dickinson, USA) and $FACSCalibur^{TM}$ (Becton Dickinson, USA). Lymphocyte subsets analysed were T ($CD3^+$) and B cells ($CD19^+$), helper/inducer T ($CD4^+$) and suppressor/cytotoxic T cells ($CD8^+$), helper/suppressor ($CD4^+/CD8^+$) ratio and natural killer (NK) cells ($CD3^-CD16^+/CD56^+$). The absolute numbers of each subset were calculated from total lymphocyte counts. Data collected was analysed using SAS 6.12. A P-value of < 0.05 was considered significant. Results: We reported the counts and percentages of lymphocyte and these subsets in each age group. There were no statistically significant differences between male and female subjects. The percentage of $CD4^+$ T cells, and the count of NK cells did not show the significant difference among the various age groups. The age-related changes observed in our study were as following: 1) a decrease in the percentages of T cells, B cells and $CD8^+$ T cells ; 2) a decrease in the counts of B cells and $CD8^+$ T cells ; 3) an increase in the percentage and count of NK cells ; and 4) an increase in the $CD4^+/CD8^+$ ratio. Conclusion: The characteristics of aging process appeared to be showing a marked decrease of lympocyte subsets T and B cells as well as T8 ($CD8^+$). The age-related increase of the percentage of cells bearing NK marker can be interpreted as a compensatory consequence to cope with the decrease of T cells related to the thymic involution. These changes with age appeared to be for the secondary study about immune cell function as lymphocyte blast transformation and immunoglobulin gene rearrangement.

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Plant regeneration and transformation of grape (Vitis labrusca L.) via direct regeneration method (포도 (Vitis labrusca L.)의 직접 재분화 방법을 이용한 식물체 재분화와 형질전환)

  • Kim, Se Hee;Shin, Il Sheob;Cho, Kang Hee;Kim, Dae Hyun;Kim, Hyun Ran;Kim, Jeong Hee;Lim, Sun-Hyung;Kim, Ki Ok;Lee, Hyang Bun;Do, Kyung Ran;Hwang, Hae Seong
    • Journal of Plant Biotechnology
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    • v.40 no.4
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    • pp.210-216
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    • 2013
  • Efficient regeneration methods and transformation system are a priority for successful application of genetic engineering to vegetative propagated plants such as grape (Vitis labrusca L.). This research is to establish shoot regeneration system from plant explants for 'Campbell Early', 'Tamnara', 'Heukgoosul', 'Heukbosek' using two types of plant growth regulators supplemented to medium. The highest adventitious shoot regeneration rate of 5% was achieved on a medium containing of Murashige and Skoog (MS) inorganic salts and Linsmaier and Skoog (LS) vitamins, 2 mg/L of TDZ and 0.1 mg/L of IBA. Leaf tissue of 'Campbell Early', was co-cultivated with Agrobacterium strains, LBA4404 containing the vector pBI121 carrying with CaMV 35S promoter, gus gene as reporter gene and resistance to kanamycin as selective agent, the other Agrobacterium strains, GV3101 containing the vector pB7 WG2D carrying with mPAP1-D gene. mPAP1-D is a regulatory genes of the anthocyanin biosynthetic pathway. 'Campbell Early' harboring mPAP1-D gene was readily able to be selected by red color due to anthocyanin accumulation in the transformed shoot. These results might be helpful for further studies to enhance the transformation efficiency in grape.

Flexible Specialization: A New Paradigm for Modern Industrial Society ? (柔軟的 專門化(Flexible Specialization) : 현대 産業社會의 새로운 패러다임 ?)

  • Lee, Deog-An
    • Journal of the Korean Geographical Society
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    • v.28 no.2
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    • pp.148-162
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    • 1993
  • There is much speculation that modern capi-talist society is undergoing fundamental and qualitative chnge towards flexible specialization. The purpose of this study is to examine this hypothesis. This paper focusses on: the idea of flexible specialization; the significance of this transition; industrial district; and the implicati-ons of this new production system for Korean industrial space. Main arguments of this study are as follows: First, as all different groups of researchers apply the idea of flexible specialization according to their own specifications, the current debate on this topic is not much fruitful. Not surpri-singly, the concept of flexible specialization has overlapped with subocontracting. This intergration of subcontracting into flexible specialization systems, however, is inappropriate because the two concepts have different historical contexts. The other cause of this controversy is its inherent weekness, conceptual ambiguity. Thus, today's flexibility becomes tomorrow's rigidity. Secondly, transition towards flexible speciali-zation has only been partially achieved even in advanced capitalist countries. The application of dualistic explanatory framework, such as rigidity versus flexibiity, mass production versus small-lot multi-product production, and de-skilling versus re-skilling, has resulted in great exaggeration of the transformation, from Fordism to post-Fordism. There is no intermediary part between two places. Considering that the workers allocated to the Fordist mass production assembly line are not as large as one might imagine, the shift from mass to flexible production has only limited implications for the transformation of capitalist economy. Thirdly, 'industrial district' contorversy has contributed to highlighting the importance of small firms and areas as production space. The agglomeration of small firms in specific areas is common in Korea, but it is quite different from the industrial district based on flexible specialization. The Korean phenomenon stems from close interactions with its major parent firm rather than interactions between flexible, specialized, autonomous and technology-intensive smll firms. Most Korean subcontractors are still low-skilled, labour-intensive, and heavily dependent on their mojor parent firms. Thus, the assertion that the Seoul Metropolitan Area adopts flexible specialization has no base. Fourthly, the main concern of flexible speciali zation is small firms. However, the corporate organization that needs product diversification and technological specialization is oligopolistic large corporations typified by multinational corporations. It is because of this that most of these organizations are adoptiong Fordist mass production methods. The problem of product diversification will be resolved naturally if economic internationalization progresses further. What is more important for business success is the quality and price competitiveness of firms rather than product diversification. Lastly, in order to dispel further misunderst-anding on this issue, it is imparative that the conceptual ambiguity is resolved most urgently. This study recommends adoption of more speci-fied and direct terminology (such as, factory automation, computer design, out-sourcing, the exploitation of part-time labor, job redesign) rather than that of ideological ones (such as, Taylorism, Fordism, neo-Taylorism, neo-Fordism, post-fordism, flexible specialization, peripheral post-Fordism). As the debates on this topic just started, we still have long way to go until consensus is reached.

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