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http://dx.doi.org/10.5010/JPB.2013.40.4.210

Plant regeneration and transformation of grape (Vitis labrusca L.) via direct regeneration method  

Kim, Se Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Shin, Il Sheob (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Cho, Kang Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Dae Hyun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Hyun Ran (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Jeong Hee (Apple Research Station, National Institute of Horticultural & Herbal Science, RDA)
Lim, Sun-Hyung (Metabolic Engineering Division, National Academy of Agricultural Science, RDA)
Kim, Ki Ok (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Lee, Hyang Bun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Do, Kyung Ran (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Hwang, Hae Seong (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Publication Information
Journal of Plant Biotechnology / v.40, no.4, 2013 , pp. 210-216 More about this Journal
Abstract
Efficient regeneration methods and transformation system are a priority for successful application of genetic engineering to vegetative propagated plants such as grape (Vitis labrusca L.). This research is to establish shoot regeneration system from plant explants for 'Campbell Early', 'Tamnara', 'Heukgoosul', 'Heukbosek' using two types of plant growth regulators supplemented to medium. The highest adventitious shoot regeneration rate of 5% was achieved on a medium containing of Murashige and Skoog (MS) inorganic salts and Linsmaier and Skoog (LS) vitamins, 2 mg/L of TDZ and 0.1 mg/L of IBA. Leaf tissue of 'Campbell Early', was co-cultivated with Agrobacterium strains, LBA4404 containing the vector pBI121 carrying with CaMV 35S promoter, gus gene as reporter gene and resistance to kanamycin as selective agent, the other Agrobacterium strains, GV3101 containing the vector pB7 WG2D carrying with mPAP1-D gene. mPAP1-D is a regulatory genes of the anthocyanin biosynthetic pathway. 'Campbell Early' harboring mPAP1-D gene was readily able to be selected by red color due to anthocyanin accumulation in the transformed shoot. These results might be helpful for further studies to enhance the transformation efficiency in grape.
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