• Title/Summary/Keyword: diode laser

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Photodynamic Therapy induced Cell Death using ALA and 632nm Diode Laser in A549 Lung Cancer Cells (A549 폐암세포주에서 ALA와 632nm Diode Laser를 이용한 광역학치료 유도성 세포사)

  • Kim, Youn Seup;Park, Jae Seuk;Jee, Young Koo;Lee, Kye Young
    • Tuberculosis and Respiratory Diseases
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    • v.56 no.2
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    • pp.178-186
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    • 2004
  • Background : Photodynamic therapy (PDT) is a new therapeutic method aimed at the selective destruction of cancer cells. The outcome is death of cancer cells through apoptosis or necrosis. The aim of this study was to investigate the characterization of PDT induced cell death in A549 lung cancer cells. Materials and methods : A549 cells were used as the lung cancer cell. 5 aminolevulinic acid (ALA) was used as the photosensitizer and a 632nm diode laser (Biolitec, Germany) as the light source. Cells were incubated with various concentrations of ALA. The 632nm diode laser was then administered for various laser irradiation times. The treated cells were incubated with 24, 48 and 72 hours. The cell viabilities were measured using the crystal violet assay and light microscopy. To observe the cell death mechanism after PDT, cells were observed under fluorescence microscopy after double staining with Hoechst 33342 and propium iodide after PDT. Results : In the crystal violet assay at 24 hours after PDT with a $3.2J/cm^2$ laser irradiation power, the cell viabilities were $89.56{\pm}4.11$, $87.67{\pm}5.48$, and $69.37{\pm}8.84$ with ALA concentrations of 10, 100, and $1mg/m{\ell}$, respectively. In crystal violet assay at 24 hours after PDT with $1mg/m{\ell}$ of ALA, the cell viabilities were $74{\pm}19.85$, $55{\pm}6.1$, and $49.06{\pm}16.64%$ with 1.6, 3.2 and $6.4J/cm^2$ laser irradiation powers, respectively. However, increasing the interval time after PDT did not change the cell viabilities. In the apoptosis assay, photodynamic therapy was inducing the apoptotic cell death. Conclusions : This study shows the apoptotic anticancer effect of photodynamic therapy in A549 lung cancer cells. However, further evaluations with other cancer cells and photosensitizers are necessary.

Ag 두께의 변화에 따른 chalcogenide layer의 회절효율 특성

  • Nam, Gi-Hyeon;Jeong, Hong-Bae
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.11a
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    • pp.197-197
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    • 2009
  • We have investigated the holographic grating formation on Ag-doped amorphous chalcogenide AsGeSeS thin films with Ag thickness. Holographic gratings have been formed using Diode Pumped Solid State laser (DPSS, 532.0nm) under [P:P] polarized the intensity polarization holography. The diffraction efficiency was obtained by +1st order intensity.

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A Study on the Metallic Surface Roughness Measurement by Laser Diode Beam (반도체 레이저 비임을 이용한 금속표면의 거칠기 측정에 관한 연구)

  • 김희남;김양술;박희재;황재연
    • Journal of the Korean Society of Safety
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    • v.7 no.4
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    • pp.73-83
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    • 1992
  • A new technique for the measurement of surface roughness based on the Intensity fluctuations of laser light backscattered form a moving surface has been introduced. The developed method will be quite useful for evaluating the surface quality under machining with more detailed Information by detecting the surface roughness along both directions simultaneously.

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Channel Orientation Dependent Electrical Characteristics of Low Temperature Poly-Si Thin-film Transistor Using Sequential Lateral Solidification Laser Crystallization

  • Lai, Benjamin Chih-ming;Yeh, Yung-Hui;Liu, Bo-Lin
    • 한국정보디스플레이학회:학술대회논문집
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    • 2007.08b
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    • pp.1263-1265
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    • 2007
  • The electrical characteristics of low temperature poly-Si (LTPS) thin-film transistors (TFT) with channel parallel and perpendicular to the direction of lateral growth were studied. The poly-Si film was crystallized using sequential lateral solidification (SLS) laser crystallization technique. The channel orientation dependent turn-on characteristics were investigated by using gated-diodes and capacitance-voltage measurements

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Analysis of Crack Behavior in a Ceramic Nd:YAG (세라믹 Nd:YAG 레이저 매질의 균열 해석)

  • Kim, Duck-Lae;Kim, Byung-Tai
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2010.06a
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    • pp.186-186
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    • 2010
  • The crack behavior in the ceramic Nd:YAG at a laser-diode end-pumped Nd:YAG ceramic laser was investigated. The fracture critical temperature difference of the ceramic Nd:YAG is about $355^{\circ}C$. The fracture of the 2 at% and the 4 at.% ceramic Nd:YAG occurred more than 14.9 W and 6.9 W pump powers, respectively, under lasing conditions.

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Optimum Power Calibration for LightScribe (라이트스크라이브(LightScribe) 미디어 라벨링(Labeling)을 위한 최적 기록 파워 조정)

  • Roh, Sang-Chul;Chung, Ki-Hyun
    • Proceedings of the IEEK Conference
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    • 2008.06a
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    • pp.1117-1118
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    • 2008
  • The LightScribe Technology is for printing images on the label side of recordable media using CD laser diode. By implementing Optimum Labeling Power Calibration for LightScribe, Labeling Quality can be improved. This paper proposes a new laser power calibration method using RFSUM signal. This function is implemented based on GH22LP20 of LG Electronics.

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Implementation of a Projection System for Projecting an Image with Laser Diodes (레이저 다이오드를 이용한 영상 투사용 프로젝션 시스템 구현)

  • Kim, Ki-Jun;Lee, Young-Chul
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • v.9 no.1
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    • pp.1157-1160
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    • 2005
  • 본 논문에서는 레이저 다이오드(Laser Diode, LD)를 배열시키고 회전 다면경(Polygon Mirror)의 반사각 및 회전다면경의 기하학적 프로젝션 시스템 설계를 통하여 2D-레이저 프로젝션 시스템을 구현하였다. 제안한 회전다면경과 레이저 다이오드 배열을 적용하석 영상 동기 시켜 레이저 배열을 적용한 레이저 디스플레이 시스템을 최종적으로 설계하고 실제 제작을 통해서 시스템을 검증하였다.

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Thermal Effect on Laser-Diode Side-Pumped Nd:YAG Laser (반도체 레이저 측면 여기 Nd:YAG 매질에서의 열영향)

  • 양동옥;김병태
    • Proceedings of the Optical Society of Korea Conference
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    • 2000.08a
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    • pp.110-111
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    • 2000
  • 여기 파워는 고체 레이저 매질 내부에 열을 발생시킨다. 매질 내부에서 발생한 열은 매질 표면을 따라 냉각이 진행되어 매질 내부에서는 불균일한 온도분포가 발생하게 된다. 레이저 매질의 굴절율은 온도에 따라 변하기 때문에 열복굴절 현상과 열렌즈 현상이 일어나 레이저 출력의 손실, 빔질의 저하를 유발하고 열적 스트레스는 매질의 손상 및 모드 동기된 극초단 펄스가 넓어지는 등의 문제를 초래한다. 선형 편광 광선을 이용하는 고체 레이저에서 열복굴절에 의해 레이저 출력이 약 30 %까지 감소하므로 레이저 공진기를 구성하는데 있어서 정량적인 열영향의 해석이 필요하다. 열복굴절에 의해 발생한 손실량은 다음과 같이 표현할 수 있다. (중략)

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A Study on the Visible Light of the Wireless Communication and Its Application (가시광 무선통신 시스템과 응용에 관한 연구)

  • Kang, Heau-Jo
    • Journal of Digital Contents Society
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    • v.8 no.4
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    • pp.425-430
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    • 2007
  • Depends on the running dry of the radio resources for the next generation of wireless communication system the estimated possibilities of the visible light which used for the devices such as signal light, the electric display board, guide board and so on applied for the next generation wireless network is the topic to be explored in this paper. Since radiation efficiency utilization for the wireless network has its capacity to become a trend direction we will study also on its application as well as the technique standardization.

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