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밤나무의 내한성(耐寒性)(제1보(第一報)) - 품종별(品種別) 부위별(部位別) 차이(差異)에 관(關)하여 - (Freezing Resistance of Chestnut (I) - The Difference among Cultivars and Tissue Parts -)

  • 조태환;홍성각
    • 한국산림과학회지
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    • 제26권1호
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    • pp.19-22
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    • 1975
  • 경기도(京畿道) 내(內) 4지역에서 1975년 3월 2일에 채취된 밤나무 10 품종(品種)에 대한 내한성도(耐寒性度)의 품종 및 부위별(部位別) 차이를 알기 위해 본 실험을 하였다. 저온(低溫) 및 해영온도(解永溫度) 처리(處理) 속도(速度)를 자연상태와 비슷한 시간당(時間當) $6^{\circ}C$ 보다 더 천천히 조절하였다. 부위별(部位別) 내한성도(耐寒性度)의 순위는 모든 품종에 있어서 동아(冬芽), 형성층(形成層), 재부유조직(材部柔組織) 및 피층(皮層)의 순위(順位)로 증가 하였다. 품종 간(間)의 내한성도의 순위는 줄기의 부위에 따라 일정치 않았으나 품종단택(品種丹澤)(단지와)은 예외적으로 모든 부위(部位)에 있어서 가장 높은 내한성도를 보였다. 가장 내한성도가 높은 품종을 발견하는데 있어서 부위별 내한성도의 계절적 변화(變化)에 관한 연구의 필요성에 관하여 고찰되었다.

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Ginsenosides analysis of New Zealand-grown forest Panax ginseng by LC-QTOF-MS/MS

  • Chen, Wei;Balan, Prabhu;Popovich, David G.
    • Journal of Ginseng Research
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    • 제44권4호
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    • pp.552-562
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    • 2020
  • Background: Ginsenosides are the unique and bioactive components in ginseng. Ginsenosides are affected by the growing environment and conditions. In New Zealand (NZ), Panax ginseng Meyer (P. ginseng) is grown as a secondary crop under a pine tree canopy with an open-field forest environment. There is no thorough analysis reported about NZ-grown ginseng. Methods: Ginsenosides from NZ-grown P. ginseng in different parts (main root, fine root, rhizome, stem, and leaf) with different ages (6, 12, 13, and 14 years) were extracted by ultrasonic extraction and characterized by Liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Twenty-one ginsenosides in these samples were accurately quantified and relatively quantified with 13 ginsenoside standards. Results: All compounds were separated in 40 min, and a total of 102 ginsenosides were identified by matching MS spectra data with 23 standard references or published known ginsenosides from P. ginseng. The quantitative results showed that the total content of ginsenosides in various parts of P. ginseng varied, which was not obviously dependent on age. In the underground parts, the 13-year-old ginseng root contained more abundant ginsenosides among tested ginseng samples, whereas in the aboveground parts, the greatest amount of ginsenosides was from the 14-year-old sample. In addition, the amount of ginsenosides is higher in the leaf and fine root and much lower in the stem than in the other parts of P. ginseng. Conclusion: This study provides the first-ever comprehensive report on NZ-grown wild simulated P. ginseng.

열화상카메라를 활용한 외부온도의 터널내 영향범위 산정방법 연구 (A Study on the Decision of Influence Range of External Temperature in the Tunnel Using Thermal Camera)

  • 이유석;이태종;박광림;오영석;차철준
    • 한국구조물진단유지관리공학회 논문집
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    • 제14권5호
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    • pp.136-143
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    • 2010
  • 터널에서 외기에 접촉되는 부분은 입, 출구부와 환기구가 있으며 이러한 부위의 열화와 그 진행속도는 외기의 영향을 받지 않는 구간과는 다른 차이를 보여준다. 실제로 터널에서 외기에 접촉되는 부분뿐만 아니라 외기의 영향을 받는 구간은 일반적인 구간과는 다른 관점에서 접근해야 할 필요가 있고 보수나 보강공법 적용시에도 우선 고려가 되어야 한다. 그럼에도 불구하고 터널의 유지관리시, 점검 및 정밀안전진단시 외부온도의 영향을 받는 구간의 결함 및 열화는 그 범위가 광범위함에도 불구하고 구조체의 안전성에 미치는 영향이 미소하다는 이유로 소홀히 되고 있는 실정이며 일시적인 계절영향, 온도 변화요인에 의한 것으로 판단하여 특별한 관리가 되지 않고 있는 실정이다. 이에 본 논문은 외부온도, 기온의 터널내 영향범위를 산출하기 위해 2개소의 도심지 터널에 대해서 적외선 열화상카메라를 활용하여 터널내의 외부온도 영향범위를 결정하고 그 영향범위에서 발생되는 콘크리트라이닝 또는 콘크리트 구조체의 결함 및 열화원인을 분석하고 유지관리시 중점을 두고 시행해야 할 사항을 제시하였다.

고려인삼과 미국삼의 부위별 Saponin 함량 (Saponin Contents of Root and Aerial Parts in Panax ginseng and Panax quinquefolium)

  • 안상득;최광태
    • 한국작물학회지
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    • 제29권4호
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    • pp.342-349
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    • 1984
  • 인삼 성분육종의 기초자료를 얻고자 동일한 생육환경하에서 생육한 4년생 이종인삼의 부위별 조사포닌 및 각 ginsenoside의 함량을 분석하였던 바 그 결과를 요약하면 다음과 같다. 1. 잎과 줄기에 함유된 조사포린 함량은 미국삼이 고려인삼인 자경종, 황숙종보다 많았으며 줄기보다는 잎에서 조사포닌함량이 높았다. 2. 뿌리의 조사포닌함량은 잎, 줄기와 같이 미국삼이 고려인삼인 자경종과 황숙종보다 높은 경향이었다. 3. 잎과 줄기 및 뿌리에 함유된 조사포닌의 panaxatriol 및 panaxadiol계 saponin의 함량비(PT/PD)는 잎과 줄기에서는 1.5~3.7:1. triol 계사포닌이 많은 반면 뿌리에서는 1:1~1.5로서 diol계사포닌이 많았다. 4. 각품종 공히 잎과 줄기에 함유된 ginsenoside종류에는 차이가 없었으나 -Rg$_2$, -Re, -Rc 등은 줄기에, -Rg$_1$, -Rd, -Rb$_1$은 잎에 다량 함유되어있었다. 5. 인삼뿌리에 함유된 ginsenoside는 -Rg$_1$, -Re, -Rb$_1$ 등이 많이 함유되었으며 고려인삼에서는 -Re가, 미국삼에서는 -Rb$_1$이 가장 많이 검출되었는데 특히 미국삼에서는 -Ro, -Ra, -Rf, -Rg$_2$가 검출되지 않았다.

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기내 배양된 반하의 전기영동에 의한 생약학적 연구 1. 전기영동법에 의한 반하 Callus의 단백질 및 효소분석 (Electrophoresis Techniques for Identification of Callus Induced from Pinella ternata (Thunb.) Breit 1. Analysis of protein and Enzymes of Callus Induced from Pinella ternata (Thunb.) Breit)

  • 최정식;류점호;박학봉;김형무
    • 한국작물학회지
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    • 제33권2호
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    • pp.122-125
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    • 1988
  • 반하의 조직부위별 배양기간에 따른 단백질 및 4종의 중요 효소에 대한 전기영동적 특성이 비교연구 결과는 다음과 같다. 1. 각 조직 유내 Callus의 단백질 pattern 은 야생식물체 각 조직과 현저한 차이가 있었다. 2. 각 조직 유내 Callus의 Esterase isozyme pattern은 야생 식물체 각 조직과 뚜렷한 차이가 있었다. 3. 각 조직 유내 4주 배양 Callus의 GOT isozyme pattern은 야생식물체 각 조직과 비슷하였으나 배양 8주 Callus에서는 분자량이 큰 새로운 isozyme band 1개가 출현하였다. 4. 각 조직 유내 4주 배양 Callus의 Peroxidase isozyme band pattern 에서는 야생식물체 총 기관에서 출현했던 1개의 분자량이 작은 band 가 나타나지 않았다.

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Influence of Plant Species and Environmental Conditions on Epiphytic and Endophytic Pink-Pigmented Facultative Methylotrophic Bacterial Populations Associated with Field-grown Rice Cultivars

  • Madhaiyan, Munusamy;Poonguzhali, Selvaraj;Sa, Tong-Min
    • Journal of Microbiology and Biotechnology
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    • 제17권10호
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    • pp.1645-1654
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    • 2007
  • The total methylotrophic population associated with rice plants from different cultivars was enumerated at three different stages: vegetative, flowering, and harvesting. The bacterial population in the leaf, rhizosphere soil, endophytic in the stem and roots, and epiphytic in the florets and grains were determined from four rice cultivars, Il-mi, Nam-pyeoung, O-dae, and Dong-jin, sampled from three different field sites. The methylotrophic bacteria isolated on AMS media containing 0.5% methanol as the sole carbon source uniformly showed three distinct morphologies, which were recorded as separate groups and their distribution among the various samples was determined using the ecophysiological index. The growth stage at the time of sampling had a more significant effect on the methylotrophic population and their distribution than the field site or cultivar. A similar effect was also observed for the PPFMs, where their population in different plant parts increased from V10 to R4 and then decreased towards stage R9. A canonical discriminant analysis of the PPFM population from different parts of rice showed clear variations among the cultivars, sampled sites, and growth stages, although the variations were more prominent among the growth stages.

Change in Levels of Vitamin U and Amino Acids in Korean Chinese Cabbages Under Various Drying Methods

  • Kim, Gun-Hee
    • 한국식품위생안전성학회지
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    • 제18권4호
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    • pp.243-250
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    • 2003
  • S-methylmethionine, vitamin U levels were affected by cultivars, parts fo Korean Chinese cabbages, and drying methods. Among drying conditions, freeze drying method appeared the best condition to maintain bitamin U content compared to oven and air drying methods. In the case of KOrean Chinese cabbages, outward leaves have high levels of chlorphyll and fiber. From this study, the outward parts contained high levels of vitamin U in two cultivars. Leaf parts were 1.1-21.2 times higher in vitamin U levels than midribs in both cultivars. This difference was shown most distinctively in freeze dried outward parts of Winter Pride cultivar. Like vitmnin U, fee amino acids also showed much higher levels in leaves. Levels of amino acids showed irregulatoy changing patterns at different parts and cultivar of Korean Chinese cabbages with various drying methods, Alanine and threonie appeared relatively aboundant amino acids in most parts of samples. Since no distinctive trends were observed in this rsult, it seems no relationship exists between amon oacids and bitamin U levels. Levels of methionine in differnent parts and cultivars of Korean Chinese cabbages dried with various methods did no show clear relationship with tlevel of vitamin U. Moreover, emthionine was not detected in freeze dired outward leaf parts which were the hithest parts of vitamin U levels in Winter Pride and 55 days cultivar. There were simila levels of methionine between overn and freeze drying. Samples prepared by air drying showed significatly lower levels than those by oven and freeze dryting. Methionine as aprecursor of vitamin U, may play a role in an increase of vitamin U during drying of KOrean Chinese cabbages.

Secondary Metabolite Profiling in Various Parts of Tomato Plants

  • Kim, Dong Sub;Na, Haeyoung;Kwack, Yurina;Chun, Changhoo
    • 원예과학기술지
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    • 제32권2호
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    • pp.252-260
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    • 2014
  • Contents of carotenoids, phenolic compounds, volatile organic compounds, and alkaloids in leaves, internodes, fruits, and roots of tomatoes in different developmental stages were measured. Lycopene, ${\beta}$-carotene, and lutein were detected in all the tested parts except roots and green fruits. Lycopene content in red fruits was $49.04{\mu}g{\cdot}g^{-1}$ FW, while that in the other parts was below $40{\mu}g{\cdot}g^{-1}$ FW. ${\beta}$-Carotene and lutein contents in 24th leaves were 5.81 and $6.40{\mu}g{\cdot}g^{-1}$ FW, respectively, and were greater than those in the other parts. Caffeic, chlorogenic, and vanillic acids were detected in all the tested parts except roots. The content of chlorogenic acid in the 18th leaves was $40.11{\mu}g{\cdot}g^{-1}$ FW, while that in the other parts was lower than $31.00{\mu}g{\cdot}g^{-1}$ FW. The contents of caffeic and vanillic acids in the 24th leaves were 9.18 and $1.64{\mu}g{\cdot}g^{-1}$ FW, respectively, and were greater than those in the other parts. Moreover, younger leaves contained the more diverse volatile organic compounds including monoterpenes and sesquiterpenes. Contents of dehydro-tomatine and ${\alpha}$-tomatine were greatest in leaves, followed by internodes, roots and fruits. Younger leaves and internodes contained more dehydro-tomatine and ${\alpha}$-tomatine than older leaves and internodes. The contents of dehydro-tomatine and ${\alpha}$-tomatine in the 24th leaves were 0.89 and $1.42mg{\cdot}g^{-1}$ FW, respectively, and were greatest among all the tested parts. Our results indicated that, except lycopene, tomato leaves included greater secondary metabolites contents than red fruits. The results suggest that inedible parts of tomato plants can be used as raw material for antioxidants, anti-inflammatory agents, fungistats, and pesticides.

Salinity Tolerance of Blackgram and Mungbean: II. Mineral Ions Accumulation in Different Plant Parts

  • Karim, M.A.;Raptan, P.K.;Hamid, A.;Khaliq, Q.A.;Solaiman, A.R.M.;Ahmed, J.U.
    • 한국작물학회지
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    • 제46권5호
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    • pp.387-394
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    • 2001
  • Blackgram (Vigna mungo) is more salt tolerant than mungbean (Vigna radiata). This study was initiated to know whether the accumulation pattern of mineral ions in different plant parts plays a significant role in the differences in salt tolerance between the two Vigna species. Different mineral ions, viz. N, Cl, Na, K, Mg and Ca in different organs of two varieties of each of blackgram- Barimash-l (susceptible one) and Barimash-2 (tolerant one), and mungbean-Barimung-3 (tolerant one) and Barimung-4(susceptible one), were analyzed after growing with 0, 50, 75 and 100 mM NaCl solutions. The two crops showed a decreased but similar pattern of total N accumulation under saline conditions. The tolerant variety of both the crops showed a less reduction in total N than the susceptible one. Leaves showed the maximum while stem the minimum N, irrespective of levels of salinity. C $l^{[-10]}$ and N $a^{+}$ accumulation increased with the increasing salinity levels. Interestingly, similar to a halophyte, the salt tolerant blackgram exhibited conspicuously higher amount of N $a^{+}$ in the shoot than the salt-susceptible mungbean. However, the tolerant varieties showed less amount of N $a^{+}$ than the susceptible one, especially in blackgram. Seeds of both Vigna spp. accumulated the minimum amount of N $a^{+}$ than other plant parts. $K^{+}$ accumulation decreased by salinity in most of the plant parts, except seeds. Blackgram showed larger reduction in K than mungbean. The $Mg^{++}$ increased in leaves, petioles and stem by salinity while decreased in the roots, podshells and seeds in both the crops. Salinity increased $Ca^{++}$ accumulation in all plant-parts except roots of both Vigna spp. Apparently, the leaves of mungbean accumulated higher concentration of $Ca^{++}$ than blackgram. Varietal differences in the accumulation pattern of $K^{+}$, $Mg^{++}$ and $Ca^{++}$ were not clear. It was concluded that blackgram, presumably, possesses a similar salt tolerance mechanism to halophyte, and the pattern of accumulation of mineral ions in blackgram and mungbean was not fully ascribed to the differences in salinity tolerance between the two Vigna species.gna species.ies.s.ies.

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재배방법이 다른 미나리의 성분 특성 (Chemical properties of Watercress(Oenanthe javanica D.C.) Depend upon Cultivating Methods)

  • 이홍렬;유맹자;정희종
    • 한국식생활문화학회지
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    • 제16권3호
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    • pp.235-242
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    • 2001
  • Chemical properties of the different parts of watercress(Oenanthe javanica D.C.) grown and harvested from the culture fields under different cultivating methods were studied. In proximate analyses of watercress in parts, moisture contained more in stem than in root or in leaf, but crude protein and crude lipid contents were lower in stem than in root or in leaf. Crude ash in root contained up to about double amount in leaf or stem. Major mineral elements detected in watercress were Fe, Mg, Ca, and K, and their contents in root was higher than those in stem or leaf. Especially, Fe in root was significantly higher than that in stem or in leaf. Total free sugar composed mainly with fructose and glucose, was the highest in watercress from Hwasoon and followed the watercress from Jeonju and Donggok in order. Free sugar content was highest in leaf and the lowest in root. Major water-soluble vitamins were vitamin C, thiamin and biotin and the content of vitamin C was higher than others. These vitamins contained more in leaf than in stem or root. Niacin contained 6.09 mg/100 g in leaf of watercress from Hwasoon, which was much higher than others, but it was not detected in stem of watercress from Jeonju. Organic acids detected were oxalic acid, citric acid and malic acid and other 12 organic acids were not detected. In fatty acid composition, there were significant differences among watercresses from different parts and different culture fields. Linoleic acid, linolenic acid and palmitic acid were major fatty acids contained in watercress and it took about 80% of the total content. Amino acid content in leaf was higher than that in root and in stem. Glutamic acid and proline were major amino acids in stem of watercress from Jeonju and in stem of watercresses from Hwasoon and Donggok, respectively. In leaves of all three watercresses glutamic acid content was the highest.

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