• Title/Summary/Keyword: dielectrophoretic

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Dielectrophoretic Technique for the Preparation of Density Gradient Polymers Doped with a Dipolar Modifier

  • Woo, Dong-Jin;Mun, Jeong-Min;Lee, Suck-Hyun;Suh, Moon-Ho
    • Macromolecular Research
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    • v.11 no.6
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    • pp.467-470
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    • 2003
  • The dielectrophoretic technique was used to prepare density gradient polymers, polystyrene doped with a dipolar modifier, diphenyl sulfide. We have measured concentration gradients of the dopant by UV/Nis spectroscopy as a function of time in a nonuniform electric field. Measured concentration data at different positions of the sample confirmed that a concentration gradient arose after a nonuniform electric field was applied to the system, these data were used to compare the concentration profile with that predicted by the dielectrophoresis equation.

Dielectrophoretic Alignment and Pearl Chain Formation of Single-Walled Carbon Nanotubes in Deuterium Oxide Solution

  • Lee, Dong Su;Park, Yung Woo
    • Carbon letters
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    • v.13 no.4
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    • pp.248-253
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    • 2012
  • Dielectrophoretic filtering and alignment of single-walled carbon nanotubes (SWCNTs) were tested using deuterium oxide as a solvent. A solution of deuterium oxide-SWCNTs was dropped on top of a silicon chip and an ac electric field was applied between pre-defined electrodes. Deuterium oxide was found to be a better solvent than hydrogen oxide for the dielectrophoresis process with higher efficiency of filtering. This was demonstrated by comparing Raman spectra measured on the initial solution with those measured on the filtered solution. We found that the aligned nanotubes along the electric field were not deposited on the substrate but suspended in solution, forming chain-like structures along the field lines. This so-called pearl chain formation of CNTs was verified by electrical measurements through the aligned tubes. The solution was frozen in liquid nitrogen prior to the electrical measurements to maintain the chain formation. The current-voltage characteristics for the sample demonstrate the existence of conduction channels in the solution, which are associated with the SWCNT chain structures.

ANALYSIS AND EXPERIMENT OF DIELECTROPHORETIC FORCE ON A CELL IN A PLANAR ELECTRODE STRUCTURE (초소형 평면 전극 구조에서 세포에 작용하는 DIELECTROPHORETIC FORCE의 해석 및 실험)

  • Choi, Jung-Hoon;Lee, Sang-Wook;Kim, Yong-Kweon
    • Proceedings of the KIEE Conference
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    • 1995.11a
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    • pp.597-599
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    • 1995
  • We have analyzed the dielectrophoretic(DEP) force on a cell in a micro planar electrode structure. We fabricate a micro planar electrode structure using micro machining technology and measure the motion of a cell that is accelerated by DEP force. DEP force on a cell is calculated by curve fitting the motion of a cell. Radish and yeast are used for the experiment. In case of radish, DEP force is increased as the voltage and the frequency is increased, and in case of yeast, DEP force is increased only as the voltage is increased DEP force on a yeast does not vary when the frequency varies from 1 MHz to 3 MHz. The result will be helpful to the manipulation of cells using DEP force.

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A Nickel Nanowire Diluter Operating through the Principle of the Dielectrophoretic Attraction Force (유전영동을 이용하는 니켈 나노와이어 희석기)

  • Yang, Jin-Ho;Yoon, Hyeun-Joong;Yang, Eui-Hyeok;Yang, Sang-Sik
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.59 no.2
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    • pp.385-389
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    • 2010
  • This paper presents a microfabricated nanowire diluter which dilutes the concentration of nanowires in solution instead of by the conventional centrifuge process. The device has 16 pairs of gold electrodes in a micro channel composed of a glass substrate and PDMS. We prepared nickel nanowires by the template-directed electrodeposition method using nanoporous anodized aluminum template (AAO). We injected the Dimethylformamide (DMF) solution containing nanowires into the inlet of the diluter while applying square wave voltages on the electrodes to trap the nanowires at the subsequent gold electrodes by means of dielectrophoretic attraction forces. The concentration of nanowires at the outlet of the micro channel was changed as we expected, which illustrates that the device can effectively dilute nanowires and can be applied to a controlled assembly of nanowires.

A Continuous Particle-size Sorter Using Negative a Dielectrophoretic Virtual Pillar Array (음의 유전영동에 의한 가상 기둥 어레이를 이용한 연속적 입자 크기 분류기)

  • Chang, Sung-Hwan;Cho, Young-Ho
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.32 no.11
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    • pp.824-831
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    • 2008
  • We present a continuous size-dependent particle separator using a negative dielectrophoretic (DEP) virtual pillar array. Two major problems in the previous size-dependent particle separators include the particle clogging in the mechanical sieving structures and the fixed range of separable particle sizes. The present particle separator uses the virtual pillar array generated by negative DEP force instead of the mechanical pillar array, thus eliminating the clogging problems. It is also possible to adjust the size of separable particles since the size of virtual pillars is a function of a particle diameter and applied voltage. At an applied voltage of 500 kHz $10\;V_{rms}$ (root mean sqaure voltage) sinusidal wave and a flow rate of $0.40\;{\mu}l\;min^{-1}$, we separate $5.7\;{\mu}m$-, $8.0\;{\mu}m$-, $10.5\;{\mu}m$-, and $11.9\;{\mu}m$-diameter polystyrene (PS) beads with separation purity of 95%, 92%, 50%, and 63%, respectively. The $10.5\;{\mu}m$- and $11.9\;{\mu}m$-diameter PS beads have relatively low separation purity of 50% and 63%. However, at an applied voltage of $8\;V_{rms}$, we separate $11.9\;{\mu}m$-diameter PS beads with separation purity over 99%. Therefore, the present particle separator achieves clog-free size-dependent particle separation, which is capable of size tuning of separable particles.

Investigation of the Binding Force between Protein A and Immunoglobulin G Using Dielectrophoretic(DEP) Tweezers Inside a Microfluidic Chip (미세유체 칩 내에서 유전영동 집게(Dielectrophoretic Tweezers) 를 이용한 단백질A와 면역 글로불린 G의 결합에 관한 연구)

  • Kwak, Tae Joon;Lee, Jae Woo;Yoon, Dae Sung;Lee, Sang Woo
    • Journal of Biomedical Engineering Research
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    • v.34 no.3
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    • pp.123-128
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    • 2013
  • The 'Dielectrophoretic Tweezers(DEP Tweezers)' can be used as a facile, economical toolkit for quantitative measurement of chemical and biological binding forces related to many biological interactions within a microfluidic device. Our experimental setup can probe the interaction between a single receptor molecule and its specific ligand. Immunoglobulin G(IgG) functionalized on polystyrene microspheres has been used to detect individual surface linked Staphylococcus protein A(SpA) molecules and to characterize the strength of the noncovalent IgG-SpA bond. It was measured and compared with the existing measurements. Measured single binding force of between Goat, Rabbit IgG and SpA were $17{\pm}7pN$, $74{\pm}16pN$. This work can be used to investigate several different ligand-receptor interactions and antigen-antibody interactions.

Development and Validation of Numerical Program for Predicting Electrokinetic and Dielectrophoretic Phenomena in a Microchannel (미소채널 내 전기역학 및 유전영동 현상 해석을 위한 수치 프로그램 개발 및 검증)

  • Kwon, Jae-Sung;Maeng, Joo-Sung;Song, Simon
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.31 no.4
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    • pp.320-329
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    • 2007
  • Electrokinesis and dielectrophoresis are important transport phenomena produced by external electric field applied to a microchannel containing a conductive fluid. We developed a CFD code to predict electrokinetic and dielectrophoretic flows in a microchannel with a uniform circular post array. Using the code, we calculated particle velocities driven by electrokinesis and dielectrophoresis, and conducted Monte Carlo simulations to visualize the particle motions. The code was validated by comparing the results with those from previous studies in literature. At a low electric field, electrokinesis and diffusion is the dominant transport mechanism. At a moderate electric field, dielectrophoresis is balanced with electrokinesis and diffusion, resulting in flowing filaments of particles in the microchannels. However, dielectrophoresis overwhelms the flow at a high electric field and traps particles locally. These results provide useful insight for optimizing design parameters of a microfluidic chip for biochemical analysis, especially for development of on-chip sample pretreatment techniques using electrokinetic and dielectrophoretic effects.

Design and Analysis of the Microfabricated Cell Fusion System using Dielectrophoretic Force (Dieletrophoresis를 이용한 초소형 세포융합시스템의 설계 및 해석)

  • Yang, Sung-Dong;Lee, Sang-Wook;Kim, Yong-Kweon
    • Proceedings of the KIEE Conference
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    • 1993.11a
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    • pp.201-203
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    • 1993
  • Recently, micro-scaled cell fusion system in the bioiogical cell fusion is preferred to Nacro-scaled one by dint of microelectronic processes. The microfabricated cell fusion system has its components such as fusion chamber, selector and detector. In this paper, we describe the design rules of the micro-fabricated cell fusion system using dielectrophoretic force and analyze its components using finite element method.

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Selective Separation of Carbon Nanostructures based on Dielectrophoresis (유전영동을 기반으로 하는 탄소 나노구조의 선택적인 분리)

  • Kang, Jun-Mo;Hong, Seung-Hyun;Choi, Jae-Boong;Kim, Young-Jin;Baik, Seung-Hyun
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.1737-1741
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    • 2008
  • Dielectrophoretic behavior of semiconducting single-walled carbon nanotubes(SWNT) was investigated theoretically and experimentally. The surface conductance of nanotubes was modulated using anionic and cationic surfactant mixtures. The experimental results indicate that dielectrophoretic behavior of SWNT highly depends on the procedure of mixing two opposite-charged surfactants. Clausius-Mossotti factor was calculated by measuring zeta potentials and solution conductivity. Raman spectroscopy was used to characterize the dielectrophoretically deposited nanotubes arrary. We found that metallic nanotubes were selectively separated from the nanotubes suspension, resulting from modulation of surface conductance of semiconducting SWNT.

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