• Food Science and Biotechnology
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• 제15권1호
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• pp.122-127
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• 2006

The effects of fermented chaga mushroom (Inonotus obliquus) powder on the lipid concentrations and the activities of liver marker enzymes of serum in genetically diabetic Otsuka Long-Evans Tokushima fatty (OLETF) rats were investigated. Rats were fed a semisynthetic diet supplemented with 50 g/kg chaga mushroom powder (CM) or 50 g/kg fermented chaga mushroom powder (FCM) for 8 weeks (26 to 34 weeks of age). Nondiabetic Long-Evans Toknshima Otsuka (LETO) rats were used as age-matched nondiabetic control animals. Water consumption was significantly higher in the OLETF control than the LETO rats. Water consumption in the FCM-fed OLETF rats tended to be less than in both the OLETF control and CM-fed OLETF rats. Serum concentrations of triglycerides and total cholesterol were significantly higher in the OLETF control rats than in the LETO rats while within the OLETF rat groups, the consumption of FCM resulted in a significantly lower serum triglyceride concentration and slightly lowered serum total cholesterol concentration when compared to the OLETF control and CM-fed rats. The alanine aminotransferase (ALT) activity was significantly higher in the OLETF control than in the LETO rats, but this difference was significantly reduced compared to the CM-fed rats and essentially no difference in the ALT levels was observed between the LETO and OLETF-FCM rats. This observation suggests an adaptive effect of the fermented chaga mushroom in liver function. Livers of the LETO rats showed no histopathological changes, whereas those of the OLETF control rats were characterized by many fat depositions in the central zone of the hepatocytes. The livers of the OLETF CM-fed rats showed less fatty changes compared to the OLETF control rats and fat deposition in the hepatocytes was nearly absent. These results suggest that orally ingested fermented chaga mushroom has a potential beneficial effect on the complications known to occur in the obesity-related non-insulin dependent diabetes mellitus (NlDDM) OLETF rat.

• Journal of Acupuncture Research
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• 제23권2호
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• pp.1-19
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• 2006

Objectives : The aim of this study was to investigate the hypoglycemic and hypolipidemic activities and mechanisms of Acanthopanax senticosus (AS) herbal acupuncture. Methods : Anti-diabetic and anti-steatotic activity of the AS herbal acupuncture was investigated on C57BL/6J ob/ob mice. After random grouping at the age of 9 weeks, the herbal acupuncture groups were injected subcutaneously at the left and right Gansu (BL18) corresponding acupuncture points alternately on exactly the same time every day with 0.1ml of either 400 mg/kg or 800 mg/kg of AS (AS400 and AS800) for 8-week period. As a positive control, metformin was administrated at a dose of 300 mg/kg (MT300). Body weights were measured weekly, and on every other week blood was collected for blood glucose analysis. At the end of study, blood was also collected for determination of plasma insulin and lipid levels, after which they were killed and periepidydimal fat, liver, muscle, and pancreas were immediately removed. The removed tissues were instantly soaked in liquid nitrogen and stored at $-70^{\circ}C$ for morphological examination and mRNA analysis. Results : The AS herbal acupuncture significantly prevented weight gain on C57BL/6J ob/ob mice. The AS herbal acupuncture lowered blood glucose and improved glucose tolerance in C57BL/6J ob/ob mice. The increase of insulin response during the OGTT was inhibited by the AS herbal acupuncture. Insulin sensitivity of skeletal tissue was enhanced. Plasma lipid levels were significantly improved in the AS herbal acupuncture groups. The AS herbal acupuncture decreased hepatic lipogenesis and hepatic triglyceride production, and increased fatty acid (FA) transporter that involves in FA uptake. The AS herbal acupuncture inhibited the increase of liver mass by prevention of the accumulation of TG but did not inhibit weight gain of fat tissue on C57BL/6J ob/ob mice. Conclusion : In summary, we have demonstrated several unique properties of the AS herbal acupuncture in decreasing body weight, and reversing insulin resistance and hepatic steatosis in ob/ob mice. This AS herbal acupuncture acts as an insulin sensitizer and specifically decreases circulating glucose and lipids, and suppresses hepatic lipogenesis.

• 대한정형도수물리치료학회지
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• 제11권2호
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• pp.62-70
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• 2005

The determination of peripheral nerve conduction velocity is an important part to electrodiagnosis. Its value as neurophysiologic investigative procedure has been known for many years but normal value of median and ulnar motor nerve was poorly reported in Korea. To evaluate of median and ulnar motor nerve terminal latency, amplitude of CMAP(compound muscle action potential), conduction velocity and F-wave latency for obtain clinically useful reference value. 71 normal volunteers(age, 19-65 years; 142 hands) examined who has no history of peripheral neuropathy, diabetic mellitus, chronic renal failure, endocrine disorders, anti-cancer medicine, anti-tubercle medicine, alcoholism, trauma, radiculopathy. Nicolet Viking II was use for detected terminal latency, amplitude of CMAP, conduction velocity and F-wave latency of median and ulnar motor nerve. Data analysis was performed using SPSS. Descriptive analysis was used for obtain mean and standard deviation, independent t-test was used to compare between Rt and Lt side also compare between different in genders. The results are summarized as follows: 1. Median motor nerve terminal latency was right 3.00ms, left 2.99ms and there was no significantly differences between right and left side and genders. 2. Median motor nerve amplitude of CMAP was right 17.26mV, left 1750mV and there was no significantly differences between right and left side and genders. 3. Median motor nerve conduction velocity was right 57.89m/sec, left 58.03m/sec and there was no significantly differences between right and left side and genders. 4. Median motor nerve F-wave latency was right 25.74ms, left 25.59ms and there was significantly differences between genders. 5. Ulnar motor nerve terminal latency was right 2.38ms, left 2.45ms and there was significantly differences between right and left side. 6. Ulnar motor nerve amplitude of CMAP was right 15.99mV, left 16.02mV and there was no significantly differences between right and left side and genders. 7. Ulnar motor nerve conduction velocity was right 60.35m/sec, left 59.73m/sec and there was no significantly differences between right and left side and genders. 8. Ulnar motor nerve F-wave latency was right 25.53ms, left 25.57ms and there was significantly differences between genders.

• 대한정형도수물리치료학회지
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• 제11권2호
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• pp.13-18
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• 2005

The ulnar nerve extends down the arm, across the elbow, and into the hand. It provides sensation to the little and ring fingers and activates many of the small muscles in the hand. The determination of peripheral nerve conduction velocity is an important part of ulnar nerve evaluation. The electrodiagnostic value as neurophysiologic investigative procedure has been known for many years but normal value of digital nerve was not reported in Korea. The purpose of this investigation was to measure the digital nerve conduction velocity of ulnar nerve for obtain clinically useful reference value and compare difference in each fingers and then compare with the other countries. 71 normal Korean volunteers (age, 19-65 years; 142 hands) examined who has no history of peripheral neuropathy, diabetic mellitus, chronic renal failure, endocrine disorders, anti-cancer medicine, anti-tubercle medicine, alcoholism, trauma, radiculopathy. Nicolet Viking II (EMG machine) was use for detected conduction velocity and amplitude of digital nerves in ulnar nerve. Data analysis was performed using SPSS. Descriptive analysis was used for obtain mean and standard deviation and independent t-test was used to compare with ring and little finger. Conduction velocity of the right ring finger was 57.44m/sec and little finger was 55.32msec. The left ring finger was 55.55msec and little finger was 54.11msec. Amplitude of the right ring finger was $30.28{\mu}V$ and little finger was $48.36{\mu}V$. The left ring finger was $30.67{\mu}V$ and little finger was $52.76{\mu}V$. There were significantly difference between ring and little in amplitude (p<.05) but there were no statistically difference between conduction velocity of ring and little finger (p>.05). The amplitude of little finger are greater than ring finger. The present results revealed that electodiagnosis can easily perform in little finger for digital nerve of ulnar nerve study.

• 한국식품과학회지
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• 제32권6호
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• pp.1418-1425
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• 2000

아가리쿠스 버섯(동결 건조, 온풍 건조)에서 추출한 ${\beta}-glucan$을 7주령의 db/db마우스에 5주간 음용수로 제공하고 식이섭취량 및 체중의 변화를 관찰하고 혈액내의 포도당, 인슐린, 당화 헤모글로빈, 소장의 glycosidase의 활성을 측정하고 중성지방농도, 혈중 지질조성을 측정하였으며 결과는 다음과 같다. db/db 마우스에서 Agari.F군과 Agari.H군은 식이섭취량에서 Control군보다 유의적으로 낮았고, 그에 따라 체중증가량도 낮았으며, 혈중 인슐린 농도와 당화헤모글로빈 농도는 Control군에 비해 Agari.F군, Agari.H군, Acarbose군이 유의적으로 낮은 수준이었다. 소장의 maltase, lactase, sucrase의 활성은 Control군에서 Lean군에 비해 세 효소의 활성이 매우 높게 관찰되었고, 시료투여군인 Agari.F군, Agari.H군은 maltase의 경우 proximal, middle 부위는 대조군보다 낮았고 distal 부위에서는 Agari.F군의 활성이 가장 높았다. Sucrase, lactase의 활성은 모든 부위에서 시료투여군이 Control군보다 낮은 수준이었다. 혈중 중성지방 농도는 Control군에 비해 Agari.F군, Agari.H군, Acarbose군은 유의적으로 낮은 수준이었다. 혈중 total cholesterol의 농도의 경우 Control군과 Agari.H군은 유의적인 차이가 없었지만 Agari.F군과 Acarbose군은 유의적으로 낮았다. 혈중 VLDL cholesterol의 경우는 Control군에 비해 Agari.F군, Agari.H군은 유의적으로 낮았고 Acarbose군은 유의적인 차이가 없었다. 혈중 LDL cholesterol의 농도는 Control군이 유의적으로 높았으며, Acarbose군이 가장 낮았다. 혈중 HDL cholesterol의 농도는 Agari.H군이 유의적으로 가장 높았고, Control군이 가장 낮았다.

• 한국자원식물학회지
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• 제23권2호
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• pp.131-137
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• 2010

본 연구에서는 타타리메밀싹을 이용한 고부가가치 가공상품을 개발하기 위하여 타타리메밀싹 발효추출물의 영양성분과 생리활성을 검정하였다. 일반 영양성분분석에서는 메밀싹 발효추출물은 단백질, 나트륨, 아연, 마그네슘에서 약간 높은 함량을 보였으나, 메밀전초 발효추출물은 나머지 무기물과 비타민에서 높게 나타났다. 동물실험을 통한 항당뇨 및 혈중지질에 관한 생리활성 검정 결과, 메밀싹 발효추출물이 짧은 기간의 실험임에도 불구하고 혈중 LDL-cholesterol의 수치를 낮추고, HDL-cholesterol의 수치를 올려주어 혈중 total lipid를 낮추는 것을 알 수 있었다. 또한 동맥경화지수인 atherogenic index의 감소를 나타내었다. 이상의 결과를 종합해 볼 때 타타리메밀싹의 발효추출물은 혈중 지질의 개선을 통해 순환기 질환에 효과를 줄 수있다는 가능성을 제시하였다. 하지만 제품화하여 메밀의 새로운 수요창출 및 부가가치를 높이기 위해서는 장기적인 동물실험 및 제품이 갖추어야 할 조건에 대한 관능검사 등 추가적인 연구를 필요로 한다.

• 한국융합학회논문지
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• 제12권7호
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• pp.69-82
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• 2021

본 연구는 국민건강영양조사 원시자료를 활용하여 혈당수준에 따른 영양상태와 식이염증지수와의 관련성을 확인하기 위해 중년여성 4,572명을 대상으로 이루어진 연구이다. 데이터는 SPSS Win 25.0 프로그램의 복합표본분석 기능의 교차분석, 일반선형모형, 로지스틱 회귀모형 등으로 분석하였으며, 결과는 다음과 같다. 혈당이 높은군(정상혈당군→당뇨군)의 대상자일수록 비만율과 혈중 TG/HDL-콜레스테롤 비율이 높게 나타난 반면 평균영양소 적정섭취비(10 종)와 두류, 종실류, 버섯류, 과일류 등의 항염증식이의 섭취량은 낮았다. 또한 식이염증지수에 대한 혈당과의 관련성 결과는, 당뇨경계 및 당뇨병의 위험도가 항염증식이 Q1에 비해 친염증식이 Q5에서 유의하게 높았다. 따라서 중년여성의 혈당 개선을 위해서는 다양한 식품의 섭취를 강조하는 교육이 효과적으로 이루어져야 함을 제안한다.

• Epidemiology and Health
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• 제39권
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• pp.36.1-36.8
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• 2017

OBJECTIVES: To investigate the screening rates for gastric, breast, and cervical cancer in people with diabetes compared with people without diabetes. METHODS: Data from the Korea National Health and Nutrition Examination Survey (2007-2009) were used. Cancer-free men who were 40 years old and over and cancer-free women who were 30 years old and over were included. The lifetime screening rate and regular screening rate were compared in people with and without diabetes. RESULTS: Fewer people with diabetes than people without diabetes had ever received cancer screening (53.5 vs. 59.5%, p<0.001 for gastric cancer; 60.5 vs. 71.5%, p<0.001 for breast cancer; and 49.1 vs. 59.6%, p<0.001 for cervical cancer). Fewer people with diabetes than people without diabetes received the recommended screenings for gastric cancer (38.9 vs. 42.9%, p<0.001), breast cancer (38.8 vs. 44.6%, p<0.001), and cervical cancer (35.1 vs. 51.2%, p<0.001). In subgroup analyses according to socioeconomic factors, the lifetime and recommended screening rates were lower in the diabetic population in most socioeconomic subgroups. In the multivariate analysis adjusted for socioeconomic factors, people with diabetes showed lower lifetime screening rates for gastric and cervical cancer (odds ratio [OR], 0.8; 95% confidence interval [CI], 0.7 to 0.9 and OR, 0.7; 95% CI, 0.6 to 0.9), and lower regular screening rates for breast and cervical cancer (OR, 0.7; 95% CI, 0.6 to 0.9 and OR, 0.7; 95% CI, 0.5 to 0.9). CONCLUSIONS: The cancer screening rate in people with diabetes was lower than in people without diabetes. Considering the higher cancer risk in people with diabetes, efforts to increase the screening rate in this high-risk population should be implemented.

• 한방비만학회지
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• 제22권2호
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• pp.93-101
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• 2022

Objectives: This study aimed to observe the anti-diabetic effect and underlying mechanisms of Galgunhwanggumhwangryun-tang (GHH; Gegen-Qinlian-decoction) in the C2C12 myotubes. Methods: GHH (1.0 mg/ml) or metformin (0.75 mM) or insulin (100 nM) were treated in C2C12 myotubes after 4 days differentiation. The glucose uptake was assessed by 2-[N-(7-160 nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose uptake by C2C12 cells. The expression of adenosine monophosphate-activated protein kinase (AMPK) and phosphorylation AMPK (pAMPK) were measured by western blot. We also evaluated gene expression of glucose transporter type 4 (Slc2a4, formerly known as GLUT4), glucokinase (Gk), carnitine palmitoyltransferase IA (Cpt1a), nuclear respiratory factors 1 (Nrf1), mitochondrial transcription factor A (Tfam), and peroxisome proliferator-activated receptor γ coactivator 1α (Ppargc1a) by quantitative real-time polymerase chain reaction. Results: GHH promoted glucose uptake in C2C12 myotubes. The expression of AMPK protein, which plays an essential role in glucose metabolism, was increased by treatment with GHH. GHH treatment tended to increase gene expression of Slc2a4, Gk, and Nrf1 but was not statistically significant. However, GHH significantly improved Tfam and Ppargc1a gene expression in C2C12 myotubes. Conclusions: In summary, GHH treatment promoted glucose uptake in C2C12 myotubes. We suggest that these effects are associated with increased gene expression involved in mitochondrial biosynthesis and oxidative phosphorylation, such as Tfam and Ppargc1a, and increased expression of AMPK protein.

• 현장농수산연구지
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• 제10권1호
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• pp.67-75
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• 2008

본 연구에서는 평창의 주요 농산물인 메밀의 새로운 수요를 창출하고 부가가치를 높이고자 음료 개발을 시도하였으며, 영양성분 조사 및 동물실험을 통한 약리적 효능을 검정하였다. 영양성분분석에서는 일반적인 성분검사로서 특별한 점이 발견되지는 않았으나 동물실험에서 밝혀진 효능검사는 타타리메밀로 만든 당절임 추출음료가 짧은 기간의 실험임에도 불구하고 혈중 LDL 콜레스테롤의 수치를 낮추고, HDL 콜레스테롤의 수치를 올려주어 혈중 total lipid를 낮추는 것을 알 수 있었다. 이상의 결과를 종합해 볼 때 타타리메밀을 이용한 음료는 혈중 지질의 개선을 통해 순환기 질환에 효과를 줄 수 있다는 가능성을 제시하였다. 하지만 제품화하여 메밀의 새로운 수요창출 및 부가가치를 높이기 위해서는 장기적인 동물실험 및 제품이 갖추어야 할 조건에 대한 관능검사를 다양한 연구가 다음 단계에서 이루어져야 한다. 본 논문에서는 타타리메밀 전초와 싹을 이용한 당절임 추출음료의 개발 가능성과 영양성분 및 기능적 효과를 밝혔다는 것에 의의가 있다. 이것은 다양한 용도의 접근 가능성을 제시하는 동시에 메밀의 다양한 수요방법을 창출할 수 있기에 농가의 부가가치를 향상시킬 수 있는 방법이 될 것이라 생각된다. 또한 타타리메밀을 이용한 당절임 추출음료가 새로운 건강식품이 될 수 있음을 전망하였다.