Fermented Chaga Mushroom (Inonotus obliquus) Effects on Hypolipidemia and Hepatoprotection in Otsuka Long-Evans Tokushima Fatty (OLETF) Rats

  • Cha, Jae-Young (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Jun, Bang-Sil (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Yoo, Ki-Soo (Department of Anatomy, College of Medicine, Dong-A University) ;
  • Hahm, Jong-Ryeal (Department of Internal Medicine, College of Medicine and Gyeongsang Institute of Health Science, Gyeongsang National University) ;
  • Cho, Young-Su (Department of Anatomy, College of Medicine, Dong-A University)
  • Published : 2006.02.28


The effects of fermented chaga mushroom (Inonotus obliquus) powder on the lipid concentrations and the activities of liver marker enzymes of serum in genetically diabetic Otsuka Long-Evans Tokushima fatty (OLETF) rats were investigated. Rats were fed a semisynthetic diet supplemented with 50 g/kg chaga mushroom powder (CM) or 50 g/kg fermented chaga mushroom powder (FCM) for 8 weeks (26 to 34 weeks of age). Nondiabetic Long-Evans Toknshima Otsuka (LETO) rats were used as age-matched nondiabetic control animals. Water consumption was significantly higher in the OLETF control than the LETO rats. Water consumption in the FCM-fed OLETF rats tended to be less than in both the OLETF control and CM-fed OLETF rats. Serum concentrations of triglycerides and total cholesterol were significantly higher in the OLETF control rats than in the LETO rats while within the OLETF rat groups, the consumption of FCM resulted in a significantly lower serum triglyceride concentration and slightly lowered serum total cholesterol concentration when compared to the OLETF control and CM-fed rats. The alanine aminotransferase (ALT) activity was significantly higher in the OLETF control than in the LETO rats, but this difference was significantly reduced compared to the CM-fed rats and essentially no difference in the ALT levels was observed between the LETO and OLETF-FCM rats. This observation suggests an adaptive effect of the fermented chaga mushroom in liver function. Livers of the LETO rats showed no histopathological changes, whereas those of the OLETF control rats were characterized by many fat depositions in the central zone of the hepatocytes. The livers of the OLETF CM-fed rats showed less fatty changes compared to the OLETF control rats and fat deposition in the hepatocytes was nearly absent. These results suggest that orally ingested fermented chaga mushroom has a potential beneficial effect on the complications known to occur in the obesity-related non-insulin dependent diabetes mellitus (NlDDM) OLETF rat.



  1. Silva GE, Takahashi MH, Eik Filho W, Albino CC, Tasim GE, Serri Lde A, Assef AH, Cortez DA, Bazotte RB. Absence of hypolipidemic effect of Solanum melongena L. (eggplant) on hyperlipidemic patients. Arq. Bras. Endocrinol. Metabol. 48: 368373 (2004)
  2. Jeon BS, Park JW, Shin Go, Kim BK, Kim HK, Cho YS, Cha JY. Effect of fermented mushroom milk on hyperlipidemia and hepatic injury in streptozotocin-induced diabetic and Zucker diabetic fatty rats. Food Sci. Biotechnol. 13: 576-580 (2004)
  3. Komoda Y, Shimizu M, Sonoda Y, Sato Y. Ganoderic acid and its derivatives as cholesterol synthesis inhibitors. Chem. Pharm. Bull. (Tokyo) 37: 531-533 (1998)
  4. Bobek P, Ozdin L, Galbavy S. Dose- and time-dependent hypocholesterolemic effect of Oyster mushroom (Pleurotus ostereatus) in rats. Nutrition 14: 282-286 (1998) https://doi.org/10.1016/S0899-9007(97)00471-1
  5. Fukushima M, Ohashi T, Fujiwara Y, Sonoyama K, Nakano M. Cholesterol-lowering effects of maitake (Grigola frondosa) fiber, shiitake (Lentinus edodes) fiber, and enokitake (Flammulina velutipes) fiber in rats. Exp. Bio. Med. (Maywood) 226: 758-765 (2001) https://doi.org/10.1177/153537020222600808
  6. Kim BK, Shin Go, Jeon BS, Cha JY. Cholesterol-lowering effect of mushrooms powder in hyperlipidemic rats. J. Korean Soc. Food Sci. Nutr. 30: 510-515 (2001)
  7. Yang BK, Park JB, Song CH. Hypolipidemic effect of an Exobiopolymer produced from a submerged mycelial culture of Hericium erinaceus. Biosci. Biotechnol. Biochem. 67: 1292-1298 (2003) https://doi.org/10.1271/bbb.67.1292
  8. Hossain S, Hashimoto M, Choubhury EK, Alam N, Hussain S, Hasan M, Choubhury SK, Mahmud I. Dietary mushroom (Pleurotus ostreatus) ameliorates atherogenic lipid in hypercholesterolaemic rats. Clin. Exp. Pharmacol. Physiol. 30: 470-475 (2003) https://doi.org/10.1046/j.1440-1681.2003.03857.x
  9. Hu SH, Wang JC, Lien JL, Liaw ET, Lee MY. Antihyperglycemic effect of polysaccharide from fermented broth of Pleurotus citrinopileatus. Appl. Microbiol. Biotechnol. 7: 1-7 (2005)
  10. Mau JL, Lin HC, Chen CC. Antioxidant properties of several medicimal mushrooms. J. Agric. Food Chem. 50: 6072-6077 (2002) https://doi.org/10.1021/jf0201273
  11. Ukawa Y, Ito H, Hisamatsu M. Antitumor effects of (1-3)-beta-Dglucan and (1-6)-beta-D-glucan purified from newly cultivated mushroom, Hatakeshimeji (Lyophyllum decastes Sing.). J. Biosci. Bioeng. 90: 98-104 (2000) https://doi.org/10.1016/S1389-1723(00)80041-9
  12. Kim YW, Kim KH, Choi HI, Lee DS. Anti-diabetic activity ofbetaglucans and their enzymatically hydrolyzed oligosaccharides from medicinal mushroom Agaricus blazei. Biotechnol. Lett. 27: 483-487 (2005) https://doi.org/10.1007/s10529-005-2225-8
  13. Diehl AM, Goodman Z, Ishak KG. Alcohol-like liver disease in nonalcoholics: a clinical and histologic comparison with alcoholinduced liver injury. Gastroenterology 95: 1056-1062 (1988) https://doi.org/10.1016/0016-5085(88)90183-7
  14. Lee EW, He P, Kawagishi H, Sugiyama K. Suppression of Dgalactosamine-induced liver injury by mushroom in rats. Biosci. Biotechnol. Biochem. 64: 2001-2002 (2000) https://doi.org/10.1271/bbb.64.2001
  15. Barbisan LF, Miyamoto M, Scolastici C, Salvadori DMF, Ribeiro LR, Eira AF, Joao Lauro Viana de Camargo. Influence of aqueous extract of Agaricus blazei on rat liver toxicity induced by different doses of diethylnitrosamine. J. Ethnopharmacol. 83: 25-32 (2002) https://doi.org/10.1016/S0378-8741(02)00171-X
  16. Zhang GL, Wang YH, Ni W, Teng HL, Lin ZB. Hepatoprotective role of Ganoderma lucidum polysaccharide against BCG-induced immune liver injury in mice. World J. Gastroenterol. 8: 728-733 (2002) https://doi.org/10.3748/wjg.v8.i4.728
  17. Shieh YH, Liu CF, Huang YK, Yang JY, Wu IL, Lin CH, Li SC. Evaluation of the hepatic and renal-protective effects of Ganoderma lucidum in mice. Am. J. Chin. Med. 29: 501-507 (2001) https://doi.org/10.1142/S0192415X01000526
  18. Cha JY, Jeon BS, Park JW, Shin GG, Kim BK, Bae DW, Yoo JH, Jeon BS, Cho YS. Antioxidative effects of mushroom extract and fermented milk containing its extract on in vivo and in vitro lipid peroxidation. J. Life Sci. 14: 514-520 (2004) https://doi.org/10.5352/JLS.2004.14.3.514
  19. Cha JY, Jeon BS, Moon JC, Yoo JH, Cho YS. Cytotoxic effect of Inonotus obliquus composition in HCT-15 human colon cancer cells and AGS gastric cancer cells. J. Korean Soc. Food Sci. Nutr. 33: 633-640 (2004) https://doi.org/10.3746/jkfn.2004.33.4.633
  20. Mau JL, Lin HC, Song SF. Antioxidant properties of several specialty mushrooms. Food Res. Int. 35: 519-526 (2002) https://doi.org/10.1016/S0963-9969(01)00150-8
  21. Benoit MA, D'Aprano G, Lacroix M. Effect of y-irradiation on phenylalanine ammonia-lyase activity, total phenolic content, and respiration of mushrooms (Agaricus bisporuss. J. Agric. Food Chem. 48: 6312-6316 (2000) https://doi.org/10.1021/jf000543s
  22. Lee EJ, Jang HD. Antioxidant activity and protective effect of five edible mushrooms on oxidative DNA damage. Food Sci. Biotechnol. 13: 443-449 (2004)
  23. Cha JY, Jeon BS, Park JW, Moon JC, Cho YS. Effect of fermented compositions containing Inonotus obliquus with Houttuynia cordata on growth of human AGS gastric and HCT-15 colon cancer cells. J.Korean Soc. Appl. Biol. Chem. 47: 202-207 (2004)
  24. Wasser SP. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbiol. Biotechnol. 60: 258-274 (2002) https://doi.org/10.1007/s00253-002-1076-7
  25. Awadh Ali NA, Mothana RAA, Lesnau A, Pilgrim H, Lindequist U. Antiviral activity of Inonotus obliquus hispidus. Fitoterapia 74: 483-485 (2003) https://doi.org/10.1016/S0367-326X(03)00119-9
  26. Kahlos K, Hiltunen R Identification of some lanostane type triterpenes from Inonotus obliquus. Acta Pharm. Fenn. 92: 220-224 (1983)
  27. Cha JY, Jun BS, Lee CH, Yu GS, Moon JC, Cho YS.Hypoglycemic and antioxidative effects of fermented chaga mushroom (Inonotus obliquus) on streptozotocin-induced diabetic rats. J. Life Sci. 15: 809-818 (2005) https://doi.org/10.5352/JLS.2005.15.5.809
  28. Kawano K, Hirashima T, Mori S, Saitoh Y, Kurosumi M, Natori T. Spontaneous long-term hyperglycemic rat with diabetic complications: Otsuka Long-Evans Tokushima Fatty (OLETF) strain. Diabetes 41: 1422-1428 (1992) https://doi.org/10.2337/diabetes.41.11.1422
  29. Jeon BS, Park JW, Kim BK, Kim HK, Jung TS, Hahm JR, Kim DR, Cho YS, Cha JY. Fermented mushroom milk supplemented dietary fiber prevents the onset of obesity and hypertriglyceridemia in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Diabetes Obes. Metab. 7: 709-715 (2005) https://doi.org/10.1111/j.1463-1326.2005.00456.x
  30. Arao K, Wang YM, Inoue N, Hirata J, Cha JY, Nagao K, Yanagita T. Dietary effect of pomegranate seed oil rich in 9cis, 11trans, 13cis conjugated linolenic acid on lipid metabolism in obese, hyperlipidemic OLETF rats. Lipids Health Dis. 3: 24-31 (2004) https://doi.org/10.1186/1476-511X-3-24
  31. Sigleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid. Am. J. Enol. Vitri. 149: 144-158 (1965)
  32. Kim YS, Kim YH, Cheon EW, Park JM, Yoo JM, Kang SS, Cho GJ, Choi WS. Retinal expression of c1usterin in the streptozotocininduced diabetic rat. Brain Res. 976: 53-59 (2003) https://doi.org/10.1016/S0006-8993(03)02636-2
  33. Duncan DB. Multiple range test for correlated and heteroscedastic means. Biometrics 13: 164-176 (1957) https://doi.org/10.2307/2527799
  34. Campbell WA, Davidson RW. A Poria as the fruiting stage of the fungus causing the sterile conks on birch. Mycologia 30: 553-560 (1938) https://doi.org/10.2307/3754349
  35. Kim YO, Han SB, Lee HW, Ahn HJ, Yoon YD, Jung JK, Kim HM, Shin CS. lmmuno-stimulating effect of the endo-polysaccharide produced by submerged culture of Inonotus obliquus. Life Sci. 77: 2438-2456 (2005) https://doi.org/10.1016/j.lfs.2005.02.023
  36. Yuan Z, He P, Cui J, Takeuchi H. Hypoglycemic effect of watersoluble polysaccharide from Auricularia auricula-judae Quel. on genetically diabetic KK-Ay mice. Biosci. Biotechnol. Biochem. 62: 1898-1903 (1998) https://doi.org/10.1271/bbb.62.1898
  37. Kannel WB, Mcgee DL. Diabetes and cardiovascular risk factors in the Framingham Study. Circulation 59: 8-13 (1993)
  38. West KM, Ahuja MM, Bennett PH, Czyzyk A, De Acosta OM, Fuller JH, Grab B, Grabauskas V, Jarrett RI, Kosaka K. The role of circulating glucose and triglyceride concentrations and their interactions with other 'risk factors' as determinants of arterial disease in nine diabetic population samples from the WHO multinational study. Diabetes Care 6: 361-369 (1983) https://doi.org/10.2337/diacare.6.4.361
  39. Gray AM, Flatt PRo Insulin-releasing and insulin-like activity of Agaricus campestris (mushroom). J. Endocrinol. 157: 259-266 (1998) https://doi.org/10.1677/joe.0.1570259
  40. Rahman SM, Wang YM, Yotsumoto H, Cha JY, Han SY, Inoue S, Yanagita T. Effects of conjugated linoleic acid on serum leptin concentration, body-fat accumulation, and $\beta$-oxidation of fatty acid in OLETF rats. Nutrition 17: 385-390 (2001) https://doi.org/10.1016/S0899-9007(00)00584-0
  41. Cheung PCK. Plasma and hepatic cholesterol levels and fecal neutral sterol excretion are altered in hamsters fed straw mushroom diets. J. Nutr. 128: 1511-1516 (1998)
  42. Man ZW, Zhu M, Noma Y, Toide K, Sato T, Asahi Y, Hirashima T, Mori S, Kawano K, Mizuno A, Sano T, Shima K. Impaired betacell function and deposition of fat droplets in the pancreas as a consequence of hypertriglycemia in OLETF rat, a model of spontaneous NIDDM. Diabetes 46: 1718-1724 (1997) https://doi.org/10.2337/diabetes.46.11.1718
  43. Lee EJ, Jang HD. Antioxidant activity and protective effect of five edible mushrooms on oxidative DNA damage. Food Sci. Biotechnol. 13: 443-449 (2004)
  44. Benoit MA, D'Aprano G, Lacroix M. Effect of $\gamma$-irradiation on phenylalanine ammonia-lyase activity, total phenolic content, and respiration of mushrooms (Agaricus bisporus). J. Agric. Food Chem. 48: 6312-6316 (2000) https://doi.org/10.1021/jf000543s
  45. Ohaeri OC. Effect of garlic oil on the levels of various enzymes in the serum and tissue of streptozotocin diabetic rats. Bioscience Rep. 21: 19-24 (2001) https://doi.org/10.1023/A:1010425932561
  46. Jia DM, Tabaru A, Akiyama T, Ade S, Otsuki M. Troglitazone prevents fatty changes of the liver in obese diabetic rats. J. Gastroentrol. Hepatol. 15: 1183-1191 (2000) https://doi.org/10.1046/j.1440-1746.2000.02316.x
  47. Yang BK, Kim DH, Jeong SC, Das S, Choi YS, Shin JS, Lee SC, Song CH. Hypoglycemic effect of a Lentinus edodes exo-polymer produced from a submerged mycelial culture. Biosci. Biotechnol. Biochem. 66: 937-942 (2002) https://doi.org/10.1271/bbb.66.937
  48. Chang IM. Liver-protective activities of aucubin derived from traditional oriental medicine. Res. Commun. Mol. Pathol. Pharmacol. 102: 189-204 (1998)
  49. Ooi VEC. Hepatoptotective effect of some edible mushrooms. Phytotherapy Res. 10: 536-538 (1996) https://doi.org/10.1002/(SICI)1099-1573(199609)10:6<536::AID-PTR893>3.0.CO;2-Y