• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.4
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• pp.355-362
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• 1999

This experiment was conducted to evaluated the effect of maturity at harvest and wrap colors on the quality of round baled rye(Secal cereale L.) silage at the forage experimental field, Grassland and Forage Crops Division, National Livestock Research Institute, Suwon in 1998. The experiment was consist of split-plot design with three repications. The main plots were three different harvesting stages : boot, heading, and flowering stages, the subplots were wrap colors : white, black, and light green color. Acid detergent fiber(ADF) and neutral detergent fiber(NDF) of rye silage were increased with delayed harvesting date, but there were no significant differdence among wrap colors. However, average in vitro dry matter digestibility (IVDMD) of rye silage with white color wrap tended to have higher than those of other colors. The silage pH of heading stage was highest (5.12), and that of flowering stage was lowest (4.57) in different harvest stages. Among tested wrap colors, white color wrap resulted on lowest pH than others (p<0.05), but there were no significant difference between black and light green color. Dry matter (DM) content of rye silage were increased as harvest stage progressed (p<0.05). However, DM content of white color was highest among warp colors, but there was signifiant difference (p<0.05). Ammonia-N content of silage harvested at boot stage was the highest in harvest stage, but there was no difference between heading and flowering stages. Among wrap colors tested, ammonia-N content of black color was highest, but there was no significant difference. The number of lactic acid bacteria of white colors was highest in different colors, and that of flowering stage was highest among harvest stage. Acetic and butyric acid contents were decreased as harvest stage progressed, and lactic acid was increased from 6.33 to 7.98%. However, wrap colors did not influence lactic acid concentration. Among different wrap colors, outside temperature of rye silage was affected by air temperature, but effect of inside temperature was minimal. Black color wrap increased inside temperature by $3{\sim}5^{\circ}C$. The results of this study indicate that rye should be harvested for round bale silage after heading stage. The quality of rye silage wrapped white and light green color will increase slightly.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.4
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• pp.347-354
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• 1999

This experiment was carried out to determine the effect of maturity at harvest and inoculants on the quality of round baled rye(Secale cereale L.) silage at the experimental field of Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon in 1998. The experiment was consist of split-plot design with 3 replications. The main plots were 3 harvesting stages such as boot(20 April), heading(29 April), and flowering stages(14 May). The subplots wered inoculant treatments : control (untreated), inoculant A, and inoculant B. Acid detergent fiber(ADF), neutral detergent fiber(NDF), and in vitro dry matter digestibility (IVDMD) of rye silage were significantly increased with delayed harvesting date, but there was not significant difference between inoculants. Mean silage pH at flowering stage was the lowest(4.35), but the highest at early harvest(4.91). Inoculants significantly reduced acidity of silage compared with control. Dry matter(DM) content of the control was higher than that of inoculants. Ammonia-N as proportion of total N was below 10% which was maximum level of high quality silage. The addition of inoculants reduced ammonia-N. There were significant difference in organic acid contents between harvesting stages and inoculants. Lactic acid was increased with inoculants, but acetic and butyric acids were decreased. Various treatments increased colony forming unit(CFU) of lactic acid bacteria by 2 or 3 times compared with the control and the highest at flowering stage with inoculant B treatment. Results of this study indicate that use of microbial inoculant and harvesting after heading stage will improve the silage fermentation and quality of round baled rye silage.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.4
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• pp.339-346
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• 1999

This experiment was carried out to determine harvest date and cultivar effects on the agronomic characteristics, forage yield, and quality of fall sown oat (Avena sativa L.) at the forage experimental field, College of Agriculture and Life Sciences, Seoul National University, Suweon from August to November 1993. The experiment was arranged in a split plot design with three replications. The main plots were consisted of the harvesting dates, suchas early (11 October), mid(21 October), and late(1 November), the subplots were consisted of different maturities of oat cultivars, such as 'West', 'Cayuse' and 'Magnum'. The first heading date of an early maturing culivar, West, was observed on 10 October, and the heading increased up to 30% at the late harvesting date. The average dry matter (DM) content of oat cultivars increased significantly from 12.8 to 15.9% as the growing stage progressed(p<0.01). The average dry matter percentage of West, Cayuse, and Magnum was 15.4, 13.4, and 13.5%, respectively. The crude protein (CP) content of oat cultivars decreased significantly from 20.1 to 13.3% as the harvesting date was delayed(p<0.01), and the CP contents of midseason and late maturing cultivars, Cayuse and Magnum, respectively, were significantly higher than that of an early maturing cultivar West (p<0.01). The mid harvesting date showed the highest acid detergent fiber(ADF) and neutral detergent fiber (NDF) contents than the other harvesting dates, and an early maturing cultivar West had higher ADF and NDF contents than the midseason and late maturing cultivars, Cayuse and Magnum, respectively. The in vitro dry matter digestibility(IVDMD) of oat cultivar West decreased as the harvesting date was delayed, but that of Cayuse and Magnum decreased only by the mid harvesting date. Cayuse and Magnum showed 3~4% higher digestibility than West. The correlation coefficient between IVDMD and ADF content was -0.86. The average dry matter yield of oat cultivars increased from 2,901 to 5,901kg/ha as the harvesting date was delayed(p<0.01), but no significant difference was found among oat cultivars. The corrdelation coefficient between DM yield and gorwing degree days(GDD) was +0.86. The results of this study indicate that a delay in harvesting date would be recommendable for the increase of dry matter yield when fall-sown oat is grown. Especially, the DM yield of the midseason and late maturing cultivars, Cayuse and Magnum, respectively, can be increased without much change of forage quality although the harvesting date are delayed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.4
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• pp.325-332
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• 1999

A manure management plan is important for all dairy operations. This experiment was conducted to determine the effect of different nitrogen(N) application rates of liquid cattle manure on the forage quality, N recovery, and total forage yields of corn-oats cropping system and soil properties at the National Livestock Research Institute, RDA, Suweon in 1997. Eight treatments consisting of no fertilizer, chemical fertilizer $320kg\;N\;ha^{-1}$ as urea, the continuous applications of 320, 640 and $960kg\;N\;ha^{-1}$ as liquid cattle manure(LCM), the residual effects of 200, 400 and $600kg\;N\;ha^{-1}$ as liquid cattle manure were arranged in a randomized complete block design with three replications. Mean plant height of fall sown oats was 70 and 61cm at the continuous application and the residual effect plots, respectively. Mean dry matter percent of fall sown oats at the residual effect plots was higher by 0.9% than that of oats at the continuous application plots, but there were no differences among all treatments. Mean crude protein(CP), acid detergent fiber(ADF), and neutral detergent fiber (NDF) contents of fall sown oats at the continuous application plots were higher by 1.0, 1.6, and 3.1%, respectively, than those of the residual effect plots. Also, there were significant differences among treatments (P<0.05). Total forage dry matter yields of corn and oats cropping system were ranged from 11,365 to $25,668kg\;ha^{-1}$ among the treatments. The yield was orderly ranked as LCM $960kg\;N\;ha^{-1}$ > LCM $600kg\;N\;ha^{-1}$ > LCM $640kg\;N\;ha^{-1}$ > LCM $400kg\;N\;ha^{-1}$ (P<0.05). Compared with the control, manurial value(MV) was 158 and 139% for the plot of the LCM $960kg\;N\;ha^{-1}$ and that of the LCM $600kg\;N\;ha^{-1}$, respectively. N recovery percent of fall sown oats was the highest at the plot of the LCM $200kg\;N\;ha^{-1}$ by 50%, and then was higher in order of the LCM $400kg\;N\;ha^{-1}$, LCM $600kg\;N\;ha^{-1}$, and LCM $320kg\;N\;ha^{-1}$. Contents of exchangeable cation in the soil of the residual effect plots was higher than that of the continuous application plots. These results suggest that the LCM $600kg\;N\;ha^{-1}$ may be the most effective in total forage dry matter yields, manurial value, N recovery, and utilizing liquid manure N under the corn and oats double cropping system.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.3
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• pp.229-236
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• 2008

This experiment was conducted to study on the evaluation of fermentation ability of microbes for whole crop rice silage Inoculant at National Institute of Animal Science, RDA from 2004 to 2005. We collected 28 strains of microbes from whole crop rice silage. According to acidity and growth ability, 5 strains of microbes was isolated (R4-1, R7-1, R7-2, R10-1, R12-1). The cultures of 4 strains were identified to be Lactobacillus plantarum (R4-1, R7-1, R7-2 and R10-1) and one was identified to be Lactobacillus pentosus (R12-1). Whole crop rice was harvested at the yellow ripen stage. It was ensiled in experimental silos (20ℓ capacity) with or without microbial additives (R4-1, R7-1, R7-2, R10-1, R12-1 and three commercial inoculant) and stored at room temperature for 60d. The pH value and acetic acid content of additivetreated silages were lower and lactic acid content was higher than those of the control (p<0.05). There was a trend for acetic acid content to be lowest and lactic acid to be highest in R7-1 treated silage. Crude protein (CP) contents of R7-2 treated silage was higher and acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents of R7-1 treated silage was lower (p<0.05). Although some strains of inoculant could improve silage quality, L. plantarum R7-1 was more effective as an inoculant for whole crop rice silage. This microbe was named NLRI 401 and registered in the Korea Agricultural Culture Collection.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.3
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• pp.215-220
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• 2008

This experiment was conducted to compare forage production and feed value of winter forage crops at the experimental field of Livestock Division, National Institute of Subtropical Agriculture from 2007 to 2008. The experiment was arranged in a randomized block design with three replications. The five winter forage crops used in this experiment were barely ('Youngyang' and 'Yuyeon'), oats ('Samhan' and 'Swan'), wheat ('Kumkang' and 'Woori'), triticale ('Shinyoung') and italian ryegrass ('Hwasan 101' and 'Florida-80'). The species having the fastest heading date was wheat (5th April) while the heading date of oats ('Samhan') was the latest as 28th April. The dry matter (DM) content of wheat was the highest among the five winter forage crops. Italian ryegrass ('Hwasan 101') showed the highest CP content by 11.2% and oats (Swan) showed the lowest content by 9.8%. Average acid detergent fiber (ADF) and neutral detergent fiber (NDF) content were 28.6 and 50.8% respectively. The five winter forage crops have narrow range of total nutrient digestibility (TDN) from 64.0% to 69.1%. Italian ryegrass ('Hwasan 101') showed the highest DM yield by 25,951 kg/ha, but the DM yield of barely and wheat low by 13,892 kg/ha ('Yuyeon') and 14,815 kg/ha ('Woori'), respectively. Italian ryegrass and oats have higher forage yield and feed value in Jeju. Also, oats has high grain and forage yield. The results demonstrated oats can cultivate as alternative winter forage crop for whole crop silage barely in Jeju.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.1
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• pp.7-12
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• 2008

This experiment was carried out to examine the winter hardiness, heading date, quality, and yields of Festulolium hybrids, Festuca pratensis and Lolium multiflorum, which were cultivated during September 2005 to July 2006 at Suwon and Namwon. The results are as follows; Perun, Boxer, and Fleurial showed stronger or same level of hardiness than Hwasan 101, which is one of the strongest hybrids in winter hardiness. Therefore, we did not detect any problem in winter hardiness when these hybrids were cultivated in Korea. These are turned out to be mid and late maturing hybrids. The average heading date of three hybrids were 24 to 27 May in Suwon and Namwon. These date are same or 3 days later than Hwasan 101. The average fresh yields of 3 hybrids were increased by $17{\sim}29%$ than that of Hwasan 101. And the dry matter yields of Perun, Boxer, and Fleurial were increased by 39%, 26%, and 29%, respectively, than that of Hwasan 101. We could not detect any difference in the average fresh and dry matter yields between region, however there were significant differences in hybrids(p<0.05). Crude protein contents of 3 hybrids were higher to $14.3{\sim}16.6%$ than that of Hwasan 101. The neutral detergent fiber and acid detergent fiber were decreased by 3.9 and 7.0%, respectively, comparing to Hwasan 101. Therefore these hybrids have turned out to be excellent feed quality hybrids.

• Journal of Yeungnam Medical Science
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• v.9 no.2
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• pp.288-301
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• 1992

The objective of the present study was to identify the characteristics of phospholipase C (PLC) isozymes purified from bovine brain and to investigate their interrelationship with G protein. The purified PLC isozymes ${\beta}$, ${\gamma}$ and ${\delta}$ were obtained and the characteristics of PLC activity on various concentrations of free $Ca^{2+}$ were observed. The activity of PLC was increased with increasing $Ca^{2+}$ concentration and the activity PLC ${\delta}$ was increased higher in the presence of phosphatidyl choline(PC) than in the abscence of PC. For vesicle formation as the structure of cell membrane, cholic acid and deoxycholic acid as detergent on phosphatidylinositol bisphosphate($PIP_2$) substrate containing PC were used, and then the activity of PLC isozymes were increased with increasing concentration of cholate, from 0.2% to 1% and were increased slightly in deoxycholate. In the $PIP_2$ containing phospholipid and glycolipid as brain extract, the activity of PLC isozymes were checked in 0.2%-1% cholic acid. The activities of PLC isoyzmes were continuously increased up to 1% cholic acid. The quantitation of PLC isozymes from several bovine organs by radioimmunoassay was made. Brain was the most sufficient organ in terms of amount of PLC ${\beta}$and ${\delta}$. A large amount of PLC ${\delta}$ was existed in adrenal gland. The binding capacity of GTPrS and G protein was observed and other observations of the binding effect of GTPrS-G protein and PLC monoclonal Ab-Protein A from tissue homogenate with PLC were made. From the observation the binding capacity was revealed the range of 0.11%-1.49%. The effects of each type of G protein on the percent activity of purified PLC isozymes were observed. From the observation, activities of isozymes were increased in $Go{\alpha}$ & Gmix, and the activities of PLC ${\beta}$ and ${\delta}$ were increased in $G{\beta}{\gamma}$ and $Gi{\alpha}$. Activities of PLC ${\beta}$ and ${\gamma}$ were decreased in $Gt{\alpha}$ but PLC ${\delta}$ increased.

• Journal of the Korea Organic Resources Recycling Association
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• v.14 no.1
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• pp.103-111
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• 2006

Fresh dairy cow manure was mixed with dried sawdust at the following moisture contents and manure: sawdust ratios: 50% and 57:43 ($\underline{M50}$), 55% and 64:36 ($\underline{M55}$), 60% and 70:30 ($\underline{M60}$), 65% and 76:24 ($\underline{M65}$), 70% and 83:17 ($\underline{M70}$) 75% and 90:10($\underline{M75}$) and 80% and 96:04($\underline{M80}$). The mixtures were fermented by a fungal mycelium of Fomitella flaxinea for 2wk at 29 C to recycle cow manure along with sawdust and fungal mycelium as a ruminant feedstuff. Chemical composition and in vitro rumen dry matter digestibilities of fermented mixtures were compared with unfermented mixture. The crude protein contents of mixtures were not changed by fermentation with fungal mycelium. Neutral detergent fiber contents of 4WK fermented mixtures (90.6, 85.3, 80.4, and 76.4% for $\underline{M50}$, $\underline{M60}$, $\underline{M70}$ and $\underline{M80}$, respectively were lower (P<0.05) than those of unfermented mixtures (91.1, 89.9, 84.3, and 79.4%). However, acid detergent fiber contents of fermented mixtures (73.8, 68.9, 65.3, and 58.0%) were higher (P<0.05) than those unfermented mixtures (70.2, 67.8, 61.7, and 56.3%). In vitro rumen dry matter digestibilities of fermented mixtures for four weeks(49.4, 36.8, 28.6, and 22.3% for $\underline{M50}$, $\underline{M60}$, $\underline{M70}$ and $\underline{M80}$) were higher than those of unfermented mixtures(34.1, 27.5, 20.6, and 15.4%) (P<0.05).

• Korean Journal of Organic Agriculture
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• v.15 no.1
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• pp.59-69
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• 2007

This experiment was conducted to study the spring productivity and feeding value of hairy vetch varieties. We also measured DM yield and feeding value by analyze CP and CF that authors made possible to calculate TDN and RFV. The results can be summarized as follows; Dry matter yield were increased earlier autumn seeding date and later cut in spring. Differences of dry matter yield in earlier cut in spring was high in order of Ostsaat, Welta, Vv4712, Penn-02, Common and Minnie. Crude protein(CP) yield was increased when earlier autumn seeding date and later cut in spring. Total digestible nutrient(TDN) yield of hairy vetch varieties was decreased when later autumn seeding date, and was increased when later cut in spring. TDN yield was highest in Ostsaat and Welta varieties had highest dry matter yield. Acid detergent fiber(ADF) content was decreased when later autumn seeding date and was increased when later cut in spring. Neutral detergent fiber(NDF) content was decreased when later autumn seeding date. Average values for relative feed value(RFV) were 157% and 132% in both cut. It shows that a high feed value in all of hairy vetch varieties. Above all, the results presented that the optimal seeding date for cultivating hairy vetch in the central region of Korea is between the 10th to the 20th of September. Because Ostsaat and Welta had significantly high dry matter yield we expected Ostsaat and Welta have a higher wintering ability.