• Title/Summary/Keyword: deposition during plant growth

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Overcoming Encouragement of Dragon Fruit Plant (Hylocereus undatus) against Stem Brown Spot Disease Caused by Neoscytalidium dimidiatum Using Bacillus subtilis Combined with Sodium Bicarbonate

  • Ratanaprom, Sanan;Nakkanong, Korakot;Nualsri, Charassri;Jiwanit, Palakrit;Rongsawat, Thanyakorn;Woraathakorn, Natthakorn
    • The Plant Pathology Journal
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    • v.37 no.3
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    • pp.205-214
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    • 2021
  • The use of the supernatant from a Bacillus subtilis culture mixed with sodium bicarbonate was explored as a means of controlling stem brown spot disease in dragon fruit plants. In in vitro experiments, the B. subtilis supernatant used with sodium bicarbonate showed a strong inhibition effect on the growth of the fungus, Neoscytalidium dimidiatum, the agent causing stem brown spot disease and was notably effective in preventing fungal invasion of dragon fruit plant. This combination not only directly suppressed the growth of N. dimidiatum, but also indirectly affected the development of the disease by eliciting the dragon-fruit plant's defense response. Substantial levels of the pathogenesis-related proteins, chitinase and glucanase, and the phenylpropanoid biosynthetic pathway enzymes, peroxidase and phenyl alanine ammonia-lyase, were triggered. Significant lignin deposition was also detected in treated cladodes of injured dragon fruit plants in in vivo experiments. In summary, B. subtilis supernatant combined with sodium bicarbonate protected dragon fruit plant loss through stem brown spot disease during plant development in the field through pathogenic fungal inhibition and the induction of defense response mechanisms.

An Experimental Study on the Direct Contamination Pathway of Elemental iodine Vapor $(I_2)$ for Rice Plants (벼에 대한 원소상 요오드 증기$(I_2)$의 직접오염경로 해석 실험)

  • Choi, Y.H.;Lim, K.M.;Lee, C.M.;Park, H.G.;Park, D.W.;Choi, G.S.;Choi, H.J.;Lee, H.S.;Lee, C.W.
    • Journal of Radiation Protection and Research
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    • v.29 no.3
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    • pp.165-172
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    • 2004
  • Rice plants were exposed to $I_2$ vapor for 80 min at different growth stages in an exposure box to investigate the parameters concerning direct plant contamination. Deposition velocity $(m\;s^{-1})$ of the $I_2$ vapor for the straws was in the range of $1.4{\times}10^{-5}-1.3{\times}10^{-4}$ depending on the exposure time, being comparatively low during the earlier part of the plant growth. Ear deposition velocity was in the range of $2.5{\times}10^{-5}-6.7{\times}10^{-5}$. Whole-plant deposition velocity was in the range of $1.4{\times}10^{-5}-1.8{\times}10^{-4}$ with the highest from the exposure performed on Aug. 18 (7 d after the start of heading). The time-dependent variation generally decreased when the deposition velocity was normalized to the biomass density No noteworthy tendency in the deposition velocity was observed with regard to the temperature, sunlight and humidity. Translocation factor for the hulled seeds was $3.3{\times}10^{-5}-4.7{\times}10^{-4}$ with the highest from the Aug. 23 exposure. It was found that a leaf deposition even before the ear emergence resulted in a considerable seed translocation.

DEVELOPMENT AND VALIDATION OF THE AEROSOL TRANSPORT MODULE GAMMA-FP FOR EVALUATING RADIOACTIVE FISSION PRODUCT SOURCE TERMS IN A VHTR

  • Yoon, Churl;Lim, Hong Sik
    • Nuclear Engineering and Technology
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    • v.46 no.6
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    • pp.825-836
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    • 2014
  • Predicting radioactive fission product (FP) behaviors in the reactor coolant system and the containment of a nuclear power plant (NPP) is one of the major concerns in the field of reactor safety, since the amount of radioactive FP released into the environment during the postulated accident sequences is one of the major regulatory issues. Radioactive FPs circulating in the primary coolant loop and released into the containment are basically in the form of gas or aerosol. In this study, a multi-component and multi-sectional analysis module for aerosol fission products has been developed based on the MAEROS model [1,2], and the aerosol transport model has been developed and verified against an analytic solution. The deposition of aerosol FPs to the surrounding structural surfaces is modeled with recent research achievements. The developed aerosol analysis model has been successfully validated against the STORM SR-11 experimental data [3], which is International Standard Problem No. 40. Future studies include the development of the resuspension, growth, and chemical reaction models of aerosol fission products.

Soil-to-Plant Transfer of $^{54}Mn,\;^{60}Co,\;^{85}Sr$ and $^{137}Cs$ Deposited during the Growing Season of Potato (감자의 재배기간 중 토양에 침적한 $^{54}Mn,\;^{60}Co,\;^{85}Sr,\;^{137}Cs$의 작물체로의 전이)

  • Choi, Yong-Ho;Lim, Kwang-Muk;Jun, In;Keum, Dong-Kwon
    • Journal of Radiation Protection and Research
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    • v.33 no.3
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    • pp.105-112
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    • 2008
  • To measure the soil-to-plant transfer factors ($TF_a,\;m^2\;kg^{-1}$-fresh) of radionuclides deposited during the growing season of potato, a radioactive solution containing $^{54}Mn,\;^{60}Co,\;^{85}Sr$ and $^{137}Cs$ was applied to the soil surfaces in soil boxes 2 d before seeding and three different times during the plant growth. For the pre-seeding application (PSA), radionuclides were mixed with the topsoil (loamy sand and 5.2 in pH). The plant parts investigated were leaves, stems, tuber skin and tuber flesh. The $TF_a$ values of $^{54}Mn,\;^{60}Co,\;^{85}Sr$ and $^{137}Cs$ from the PSA were in the ranges of $1.9{\times}10^{-4}{\sim}1.5{\times}10^{-2}$, $1.8{\times}10^{-4}{\sim}7.5{\times}10^{-4}$, $4.0{\times}10^{-4}{\sim}1.6{\times}10^{-2}$, $1.5{\times}10^{-4}{\sim}3.9{\times}10^{-4}$ respectively, for different plant parts. The TFa values from the growing-time applications were on the whole a few times lower than those from the PSA. For $^{54}Mn,\;^{85}Sr$ and $^{137}Cs$, the $TF_a$ values from the early- or middle-growth-stage application were higher than those from the late-growth-stage application, whereas the opposite was true for $^{60}Co$. Leaves and tuber flesh had the highest and lowest $TF_a$ values, respectively, in most cases. The total uptake from soil by the four plant parts was in the range of $0.05{\sim}3.16%$. In the third year following the PSA, the $TF_a$ values of $^{54}Mn,\;^{60}Co$ and $^{137}Cs$ were $11{\sim}25%$, $21{\sim}25%$ and $38{\sim}67%$ of those in the first year, respectively, depending on the plant parts. The present results can be used for estimating the radiological impact of an acute radioactive deposition during the growing season of potato and for testing the validity of relevant food-chain models.

The DISNY facility for sub-cooled flow boiling performance analysis of CRUD deposited zirconium alloy cladding under pressurized water reactor condition: Design, construction, and operation

  • Ji Yong Kim;Yunju Lee;Ji Hyun Kim;In Cheol Bang
    • Nuclear Engineering and Technology
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    • v.55 no.9
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    • pp.3164-3182
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    • 2023
  • The CRUD on the fuel cladding under the pressurized water reactor (PWR) operating condition causes several issues. The CRUD can act as thermal resistance and increases the local cladding temperature which accelerate the corrosion process. The hideout of boron inside the CRUD results in axial offset anomaly and reduces the plant's shutdown margin. Recently, there are efforts to revise the acceptance criteria of emergency core cooling systems (ECCS), and additionally require the modeling of the thermal resistance effect of the CRUD during the performance analysis. There is an urgent need for the evaluation of the effect of the CRUD deposition on the cladding heat transfer under PWR operating conditions, but the experimental database is very limited. The experimental facility called DISNY was designed and constructed to analyze the CRUD-related multi-physical phenomena, and the performance analysis of the constructed DISNY facility was conducted. The thermal-hydraulic and water chemistry conditions to simulate the CRUD growth under PWR operating conditions were established. The design characteristics and feasibility of the DISNY facility were validated by the MARS-KS code analysis and separate performance tests. In the current study, detailed design features, design validation results, and future utilization plans of the proposed DISNY facility are presented.

Silencing of the Target of Rapamycin Complex Genes Stimulates Tomato Fruit Ripening

  • Choi, Ilyeong;Ahn, Chang Sook;Lee, Du-Hwa;Baek, Seung-A;Jung, Jung Won;Kim, Jae Kwang;Lee, Ho-Seok;Pai, Hyun-Sook
    • Molecules and Cells
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    • v.45 no.9
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    • pp.660-672
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    • 2022
  • The target of rapamycin complex (TORC) plays a key role in plant cell growth and survival by regulating the gene expression and metabolism according to environmental information. TORC activates transcription, mRNA translation, and anabolic processes under favorable conditions, thereby promoting plant growth and development. Tomato fruit ripening is a complex developmental process promoted by ethylene and specific transcription factors. TORC is known to modulate leaf senescence in tomato. In this study, we investigated the function of TORC in tomato fruit ripening using virus-induced gene silencing (VIGS) of the TORC genes, TOR, lethal with SEC13 protein 8 (LST8), and regulatory-associated protein of TOR (RAPTOR). Quantitative reverse transcription-polymerase chain reaction showed that the expression levels of tomato TORC genes were the highest in the orange stage during fruit development in Micro-Tom tomato. VIGS of these TORC genes using stage 2 tomato accelerated fruit ripening with premature orange/red coloring and decreased fruit growth, when control tobacco rattle virus 2 (TRV2)-myc fruits reached the mature green stage. TORC-deficient fruits showed early accumulation of carotenoid lycopene and reduced cellulose deposition in pericarp cell walls. The early ripening fruits had higher levels of transcripts related to fruit ripening transcription factors, ethylene biosynthesis, carotenoid synthesis, and cell wall modification. Finally, the early ripening phenotype in Micro-Tom tomato was reproduced in the commercial cultivar Moneymaker tomato by VIGS of the TORC genes. Collectively, these results demonstrate that TORC plays an important role in tomato fruit ripening by modulating the transcription of various ripening-related genes.

Deposition Velocity of Iodine Vapor ($(I_2)$) for Radish Plants and Its Root-Translocation Factor : Results of Experimental Exposures (요오드 증기($I_2$)의 무 작물체에 대한 침적속도 및 뿌리 전류계수 : 피폭실험 결과)

  • Choi, Yong-Ho;Lim, Kwang-Muk;Jun, In;Park, Doo-Won;Keum, Dong-Kwon
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.8 no.2
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    • pp.151-158
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    • 2010
  • In order to measure the deposition velocity of $I_2$ vapor for radish plants and its translocation factor for their roots, radish plants were exposed to $I_2$ vapor for 80 min. at different growth stages between 29 and 53 d after sowing. The exposure was performed in a transparent chamber during the morning time. Deposition velocities ($ms^{-1}$) were on the whole in the range of $1.0{\times}10^{-4}{\sim}2.0{\times}10^{-4}$ showing an increasing tendency with an increase in the biomass density. The results showed some agreement with existing reports that a higher relative humidity would lead to a higher deposition velocity. The acquired deposition velocities were lower than by factors of several tens than some field measurements probably due to a very low wind speed (about $0.2\;ms^{-1}$) in the chamber. Translocation factors (ratio of the total iodine in the roots at harvest to the total plant deposition), estimated in a more or less conservative way, were $1.3{\times}10^{-3}$ for an exposure at 29 d after sowing and $5.0{\times}10^{-3}$ for an exposure at 53 d after sowing. In using the present experimental data, meteorological conditions and chemical and physical forms of iodine need to be carefully considered.

Soil-to-Plant Transfer Factors and Migration of Radionuclides Applied onto Soli during Growing Season of Cucumber (오이의 재배기간중 처리한 방사성 핵종의 토양;작물체간 전이계수 및 지하이동)

  • Choi, Yong-Ho;Park, Hyo-Kook;Kim, Sang-Bog;Choi, Geun-Sik;Lee, Jeong-Ho
    • Korean Journal of Environmental Agriculture
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    • v.16 no.4
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    • pp.304-310
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    • 1997
  • In greenhouse, a mixed solution of Mn-54, Co-60, Sr-85 and Cs-137 was applied to the soil of culture boxes 2 days before sowing cucumber and at 4 different times during its growth for measuring their transfer factors (TFs) for fruit and migration in soil. TFs varied with radionuclide, application time and harvest time by factor of up to about 60. Variations in TFs with application time showed different patterns among radionuclides. TFs decreased on the whole in the order of Sr-85 > Mn-54 > Co-60 > Cs-137. TFs of Mn-54, Co-60 and Cs-137 mixed with topsoil before sowing were a little higher than those for the soil-surface application made at an early growth stage while no difference in Sr-85 TF was found. After harvest, soil concentrations of the radionuclides applied at an early growth stage were examined. They decreased with increasing soil depth and 80${\sim}$99% of the radioactivity remained in the top 3cm. Soil pemeation of the radionuclides migration decreased in the order of Sr-85 > Mn-54 > Co-60 > Cs-137. The present data can be utilized in estimating radionuclide concentration in cucumber fruit, taking proper measures for its harvest and consumption and designing the best way of soil decontamination following an radioactive deposition during the cucummber growing season.

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Underground Migration of $^{54}Mn,\;^{60}Co,\;^{85}Sr\;and\;^{137}Cs$ Deposited during the Growth of Major Crop Plants (주요 작물의 생육중에 침적한 $^{54}Mn,\;^{60}Co,\;^{85}Sr,\;^{137}Cs$ 의 지하이동)

  • Choi, Yong-Ho;Jo, Jae-Seong;Lee, Chang-Woo;Lee, Myung-Ho;Kim, Sang-Bog;Hong, Kwang-Hee;Choi, Geun-Sik;Lee, Jeong-Ho
    • Journal of Radiation Protection and Research
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    • v.21 no.1
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    • pp.51-58
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    • 1996
  • Underground migration of $^{54}Mn,\;^{60}Co,\;^{85}Sr\;and\;^{137}Cs$ in paddy and upland conditions was studied through two years' greenhouse experiment. At early and late growth stages of rice, soybean, Chinese cabbage and radish, a mixed solution of the radionuclides was applied to the water or soil surfaces of the culture boxes filled with an acidic loamy-sandy soil for the upper 20cm. Soil was sampled in layers upto $15{\sim}20cm$ down after harvest. Soil concentrations of the radionuclides decreased exponentially with increasing soil depth and more than 80% of the radioactivities remained in top $3{\sim}4cm$. The mobility of the radionuclides decreased in the order of $^{85}Sr>^{54}Mn>^{60}Co{\geq}^{137}Cs$. Downward migrations of the radionuclides were the greatest in rice soil and the lowest in soybean soil which was fertilized with the least amount of N, P and K. Differences in depth profiles between two application times indicate that the amount of daily migration from $0{\sim}1cm$ layer to the lower area decreases with increasing time after deposition. By a simultaneous addition of KCl and lime following the earlier application, downward migration in soybean, Chinese cabbage and radish soils changed little or retarded more or less but that in rice soil accelerated a little.

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Root Uptake of $^{54}Mn,\;^{60}Co,\;^{85}Sr\;and\;^{137}Cs$ Deposited at Different Times during the Growing Season of Rice (벼 재배 기간중 침적시기에 따른 Root Uptake of $^{54}Mn,\;^{60}Co,\;^{85}Sr,\;^{137}Cs$의 뿌리흡수)

  • Choi, Yong-Ho;Jo, Jae-Seong;Lee, Chang-Woo;Hong, Kwang-Hee;Lee, Jeong-Ho
    • Journal of Radiation Protection and Research
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    • v.20 no.4
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    • pp.255-263
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    • 1995
  • Root uptakes of $^{54}Mn,\;^{60}Co,\;^{85}Sr\;and\;^{137}Cs$ by rice were studied through a greenhouse experiment in which the upper 20 cm of the culture box was filled with an acidic loamy-sandy soil and a mixed solution of the radionuclides was applied to the surface water on the soil 2 days before, and 5 different times after, transplanting. Percent uptakes of the radionuclides to rice tops varied $3.4{\sim}13.7%,\;0.03{\sim}0.1%,\;0.6{\sim}1.5%,\;0.02{\sim}0.15%$, respectively, with application time. Among radionuclides, soil-to-plant transfer factors decreased, on the whole, in the order of $^{54}Mn>^{85}Sr>^{60}Co{\geq}^{137}Cs$, and among plant parts, in the order of straw > chaff > hulled seed. Transfer factors $(m^2/kg-dry)$ in hulled seed were, depending on application time, $1.2{\times}10^3{\sim}5.0{{\times}10^3\;for\;^{54}Mn,\;1.6{\times}10^5{\sim}2.6{\times}10^4\;for\;^{60}Co,\;1.1{\times}10^4{\sim}7.6{\times}10^4\;for\;^{85}Sr\;and\;5.2{\times}10^5{\sim}7.0{\times}10^4\;for\;^{137}Cs$. The highest factors of all the radionuclides in straw came from the application at 67 days after transplanting while those of $^{54}Mn,\;^{60}Co\;and\;^{85}Sr$ in hulled seed appeared at later applications. The data from this studv can be referred to in assessing the radiological impact of an accidental contamination during the rice growth.

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