• Food Science of Animal Resources
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• v.26 no.3
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• pp.402-409
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• 2006

Although microorganisms are, in fact, the most diverse and abundant type of organism on Earth, the ecological functions of microbial populations remains poorly understood. A variety of bacteria including marine Vibrios encounter numerous ecological challenges, such as UV light, predation, competition, and seasonal variations in seawater including pH, salinity, nutrient levels, temperature and so forth. In order to survive and proliferate under variable conditions, they have to develop elaborate means of communication to meet the challenges to which they are exposed. In bacteria, a range of biological functions have recently been found to be regulated by a population density-dependent cell-cell signaling mechanism known as quorum-sensing (QS). In other words, bacterial cells sense population density by monitoring the presence of self-produced extracellular autoinducers (AI). N-acylhomoserine lactone (AHL)-dependent quorum-sensing was first discovered in two luminescent marine bacteria, Vibrio fischeri and Vibrio harveyi. The LuxI/R system of V. fischeriis the paradigm of Gram-negative quorum-sensing systems. At high population density, the accumulated signalstrigger the expression of target genes and thereby initiate a new set of biological activities. Several QS systems have been identified so far. Among them, an AHL-dependent QS system has been found to control biofilm formation in several bacterial species, including Pseudomonas aeruginosa, Aeromonas hydrophila, Burkholderia cepacia, and Serratia liquefaciens. Bacterial biofilm is a structured community of bacterial cells enclosed in a self-produced polymeric matrix that adheres to an inert or living surface. Extracellular signal molecules have been implicated in biofilm formation. Agrobacterium tumefaciens strain NT1(traR, tra::lacZ749) and Chromobacterium violaceum strain CV026 are used as biosensors to detect AHL signals. Quorum sensing in lactic acid bacteria involves peptides that are directly sensed by membrane-located histidine kinases, after which the signal is transmitted to an intracellular regulator. In the nisin autoregulation process in Lactococcus lactis, the NisK protein acts as the sensor for nisin, and NisR protein as the response regulator activatingthe transcription of target genes. For control over growth and survival in bacterial communities, various strategies need to be developed by which receptors of the signal molecules are interfered with or the synthesis and release of the molecules is controlled. However, much is still unknown about the metabolic processes involved in such signal transduction and whether or not various foods and food ingredients may affect communication between spoilage or pathogenic bacteria. In five to ten years, we will be able to discover new signal molecules, some of which may have applications in food preservation to inhibit the growth of pathogens on foods.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.444-451
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• 1999

The effect of individual density on the growth and survival of Laminaria japonica was experimentally evaluated. Four density treatments of young sporophytes (0.63 cm In maximum length) grown in the laboratory were transplanted to the southeastern coast of Korea and their survivorship and growth rate were measured twice a month from January to July, 1996. Plants showed higher survivorship at low density. Final densities at three lowest densities were similar, ranging from 34 to 38 inds${\cdot}100 cm^{-2}$. Planis at the highest density, however, suffered greater decline than at lower densities, and only 21 plants remained at the end of this study. Mean frond size (length and width) fluctuated throughout the study period at four densities. During the first two mouths after outplanting, mean frond size was negatively correlated with initial density, However, the size was density independent after three months. As stand density increased, size-frequency distribution through time tended to be more positively skewed. The 10 largest plants grew faster in frond size and attained larger size in the lower density. There was a positive relationship between plant size and relative growth rate (or survival). The slope of yield-density (Y-D) relationships at four densities ranged from -0.060 to -0.137. The study suggests that density can strongly affect the survival and growth of this alga. The density-dependent survival and growth patterns, however, were not strictly consistent with those of terrestrial plants.

• ETRI Journal
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• v.15 no.2
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• pp.11-25
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• 1993

Interface trap densities at gate oxide/silicon substrate ($SiO_2/Si$) interfaces of metal oxide semiconductor field-effect transistors (MOSFETs) were determined from the substrate bias dependence of the subthreshold slope measurement. This method enables the characterization of interface traps residing in the energy level between the midgap and that corresponding to the strong inversion of small size MOSFET. In consequence of the high accuracy of this method, the energy dependence of the interface trap density can be accurately determined. The application of this technique to a MOSFET showed good agreement with the result obtained through the high-frequency/quasi-static capacitance-voltage (C-V) technique for a MOS capacitor. Furthermore, the effective substrate dopant concentration obtained through this technique also showed good agreement with the result obtained through the body effect measurement.

• Food Science and Biotechnology
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• v.17 no.2
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• pp.413-416
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• 2008

Effects of extracts obtained from the flowers of Elsholtzia splendens on the serum lipid profile and hepatotoxicity in mice were investigated. Female ICR mice were given E. splendens ethanolic extract (ESEs) orally at a dose of 10 or 50 mg/kg BW for 50 days. Significant dose-dependent decreases in triglyceride and low-density lipoprotein (LDL)-cholesterol of serum were observed. In addition, ESEs prolonged the lag-time of LDL oxidation in vitro. In the serum of ICE mice given ESEs orally at 10 and 50 mg/kg BW, the serum levels of aspartate aminotransferase (AST) and lactic dehydrogenase (LDH) increased significantly, while total protein, albumin, creatinine, alanine aminotransferase (ALT), and total bilirubin did not change. Therefore, ESEs may be beneficial to human health, although it has some hepatotoxicity.

• Geomechanics and Engineering
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• v.8 no.4
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• pp.595-613
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• 2015

An experimental investigation into the uplift capacity of horizontal square plate anchors in sand with and without geogrid reinforcement is reported. The parameters investigated are the effect of the depth of the single layer of geogrid, vertical spacing of geogrid layers, number of geogrid layers, length of geogrid layers, the effects of embedment depth, and relative density of sand. A series of three dimensional finite element analyses model was established and confirmed to be effective in capturing the behaviour of plate anchor-reinforced sand by comparing its predictions with experimental results. The results showed that the geogrid reinforcement had a considerable effect on the uplift capacity of horizontal square plate anchors in sand. The improvement in uplift capacity was found to be strongly dependent on the embedment depth and relative density of sand. A satisfactory agreement between the experimental and numerical results on general trend of behaviour and optimum geometry of reinforcement placement is observed. Based on the model test results and the finite element analyses, optimum values of the geogrid parameters for maximum reinforcing effect are discussed and suggested.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2003.10a
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• pp.347-354
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• 2003

The dynamics of fluid flow through nanomachines is completely different from that of continuum. In this study, molecular dynamics simulations were performed for the flow of helium, neon, argon inside carbon(graphite) nanotubes of several sizes. The fluid was introduced into the nanotube at a given initial velocity according to given temperature. Diffusion coefficients were evaluated by Green-Kubo equation derived from Einstein relationship. The behaviour of the fluid was strongly dependent on the density of fluid and tube diameter, not on the tube length. It was found that the diffusion Coefficients increased With decreasing the density of molecules and increasing the diameter and temperature.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.800-807
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• 2001

This study was designed to investigate the antioxidative activity of a substance isolated from. The antioxidative activity of procyanidine was higher than that of dl-tocopherol and BHA on low-density lipoprotein (LDL) oxidation by thiobarbituric acid reactive substance (TBARS). Procyanidine inhibited the copper-mediated oxidation of human LDL in a dose dependent manner with almost complete inhibition at $60{\mu}g/mL$. Procyanidine at a concentration of $80{\mu}g/mL$ in hibited oxidation of LDL induced by J774. LDL oxidized by copper-mediated or cell-induced oxidation was degraded at a much greater rate than native LDL. These results suggested the importance of further research to procyanidine in the investigation of atherosclerosis and free radical-induced injury.

• KSBB Journal
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• v.13 no.3
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• pp.272-278
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• 1998

Cell recovery from high cell density broths of Alcaligenes eutrophus by pretreatment with aluminum-based coagulants such as aluminum sulfate, polyaluminum hydrooxide chloride silicate (PACS), and polyaluminum hydrooxide chloride (Hi-PAX) was carried out. Cells coagulated with coagulants could be successfully recovered above 95-99% by centrifugation or filtration. The optimum initial pH of fermentation broths for cell recovery was in the range of 10 to 12. Optimum coagulants dosage for cell recovery increased with increasing of cell concentrations (21-160 g/L). The optimum coagulant dosages to recover cells with more than 95% cell recovery by centrifugation for the cell concentrations ranged 21-160 g/L were as follows: aluminum sulfate, 416-1708 mg Al/L; PACS, 211-826 mg Al/L; Hi-PAX, 320-960 mg Al/L. At optimum conditions for the coagulation of cells, centrifugal forces for 95% of cell recovery were dependent on the cell concentration. The centrifugal forces at 82 g/L and 160 g/L of cell concentration were only 45${\times}$g and 1600${\times}$g, respectively.

• KSBB Journal
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• v.17 no.3
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• pp.307-310
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• 2002

In order to obtain high density seed cells for biofiltration, we studied batch and fed-batch culture of P. putida. Studies were carried out to find optimum fermentation conditions such as pH, concentration of glucose and agitation speed. Specific growth rate of P. putida was dependent on agitation speed and a high rpm of 300 was necessary to carry out the efficient aerobic growth of P. putida. Specific growth rate was highest at pH 7. Feeding glucose and yeast extract continuously at the initial growth phase was the most effective way to get high cell density of P. putida.

• Transactions of the Korean Society of Automotive Engineers
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• v.4 no.5
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• pp.179-186
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• 1996

The magnetic fibrous polymeric oil filter for automotive was obtained by pneumoextrusion processing prepared from thermoplastic polymer(polyamide) containing magnetic particulate filer (Ba ferrite), and treated subsequently in a magnetic fields. Using an oil filter tester and particle quantifier, the dependence of filtrational characteristics of the magnetic oil filter on the parameters of mean pore sizes and magnetic properties were analyzed. Also experimental engine-bed test of oil filters was investigated. The magnetic fibrous polymeric filter was shown to possess a highly filtration efficiency in filtering the fine metal particles with increasing the magnetic force of filter element. In this study, we knew that efficiency of magnetic fibrous polymeric filter was dependent on the magnetic strength of the filter elements.