• Journal of Animal Science and Technology
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• 제63권5호
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• pp.1182-1193
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• 2021

The aims of this study were to develop a milk protein-based probiotic delivery system using a modified rennet-induced gelation method and to determine how the skim milk powder concentration level and pH, which can affect the rennet-induced intra- and inter-molecular association of milk proteins, affect the physicochemical properties of the probiotic delivery systems, such as the particle size, size distribution, encapsulation efficiency, and viability of probiotics in simulated gastrointestinal tract. To prepare a milk protein-based delivery system, skim milk powder was used as a source of milk proteins with various concentration levels from 3 to 10% (w/w) and rennet was added to skim milk solutions followed by adjustment of pH from 5.4 or 6.2. Lactobacillus rhamnosus GG was used as a probiotic culture. In confocal laser scanning microscopic images, globular particles with a size ranging from 10 ㎛ to 20 ㎛ were observed, indicating that milk protein-based probiotic delivery systems were successfully created. When the skim milk powder concentration was increased from 3 to 10% (w/w), the size of the delivery system was significantly (p < 0.05) increased from 27.5 to 44.4 ㎛, while a significant (p < 0.05) increase in size from 26.3 to 34.5 ㎛ was observed as the pH was increased from 5.4 to 6.4. An increase in skim milk powder concentration level and a decrease in pH led to a significant (p < 0.05) increase in the encapsulation efficiency of probiotics. The viability of probiotics in a simulated stomach condition was increased when probiotics were encapsulated in milk protein-based delivery systems. An increase in the skim milk powder concentration and a decrease in pH resulted in an increase in the viability of probiotics in simulated stomach conditions. It was concluded that the protein content by modulating skim milk powder concentration level and pH were the key manufacturing variables affecting the physicochemical properties of milk protein-based probiotic delivery systems.

• Korean Chemical Engineering Research
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• 제57권6호
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• pp.898-906
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• 2019

본 연구에서는 전기천공시스템에서 형광 염료를 사용한 세포 생존율 평가와 형광 염료를 전달 물질로 하여 세포 내부로의 전달 효율 평가를 통해 세포의 생존율과 전달 물질의 전달 효율 평가에 사용하기 적합한 형광 염료의 선정 및 사용에 적절한 농도에 대한 가이드라인을 제시 할 수 있는 기초 연구를 수행하였다. 형광 염료로는 Propidium Iodide와 Yo-Pro-1을 사용하였다. Propidium Iodide과 Yo-Pro-1을 사용한 세포 생존율 평가와 전달 효율의 평가에서 각 형광염료의 유세포 분석 히스토그램의 모양은 형광 염료의 종류와 사용한 농도에 따라 다른 것을 확인하였다. 이는 사용하는 형광 염료의 특성과 연관이 있는 것으로 이 결과를 통해 세포 분석에 사용하는 형광 염료의 특성에 따라 분석 결과가 달라지는 것을 알 수 있었다. 형광 염료 자체가 세포 생존률에 미치는 영향은 크지 않음을 확인하였다. 또한 두 종류 이상의 형광 염료를 사용하여 분석을 하는 경우 발생할 수 있는 형광 신호 간의 간섭 영향을 확인하였다. 간섭의 영향은 Yo-Pro-1의 농도가 높을수록 큰 것으로 확인되었으며 Propidium Iodide의 농도는 형광 신호의 간섭에 큰 영향을 미치지 않음을 확인하였다. 본 연구를 통해 형광 염료의 종류와 농도에 따라 세포 생존율 평가와 전달 효율의 평가 결과가 영향을 받는 것을 확인했으며 이는 형광 염료의 특성과 연관이 있는 것으로 판단된다. 또한, 본 연구 결과를 토대로 세포 생존율 평가와 전달 효율 평가에 적절한 형광 염료의 선정과 사용에 적절한 농도를 제시하였다.

• 유통과학연구
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• 제18권4호
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• pp.39-50
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• 2020

Purpose: This paper aimed to study the current locations of post offices to analyze service coverage area for parcel delivery in the Eastern Economics Corridor (EEC), which must be considered in the last mile to extend delivery service for e-commerce growth. Thailand Post was the case study in this paper. Research design, data and methodology: To involve solving the delivery service area under the last mile condition, the authors proposed a network analysis to determine service radius by employing a Geographic Information System (GIS). Furthermore, this paper applied Dijkstra's algorithm as a network analysis tool from GIS for analyzing the last mile service coverage area in a new economics zone. At the same time, the authors suggested an approach as a solution to locate last mile delivery center in EEC. Results: The results of the study pointed out that Thailand Post should consider more last mile delivery centers in EEC to support its express service in urban areas as well as improve the efficiency of service coverage for parcel delivery and create more advantages against competitors. Conclusions: This paper proposes a network analysis to extend the last mile service for parcel delivery by following Dijkstra's algorithm from GIS and a solution approach to add more last mile delivery centers. The results of the research will contribute to boosting customer satisfaction for last mile delivery service and enabling easy accessibility to a service center in EEC.

• 한국통신학회논문지
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• 제30권4C호
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• pp.262-268
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• 2005

웹기반의 디지털콘텐트 전송은 웹 객체를 단위로 이루어진다. 따라서 웹기반 콘덴트 전송의 성능을 향상시키기 위해서는 웹 객체의 참조특성 분석을 통한 반영이 필요하다. 본 연구에서는 웹 객체의 참조특성의 분석을 통해 웹 객체의 전송 효율을 향상시킨 디지털콘텐트 전송 시스템을 설계하고 성능을 평가하였다. 제안시스템은 웹 객체의 참조특성을 반영하여, 캐싱 효율을 높임으로서 전체 시스템의 처리지연시간을 개선하는 것을 목적으로 하고 있으며 실험 결과 제안시스템의 성능이 우수함을 알 수 있었다.

• 한국인터넷방송통신학회논문지
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• 제10권4호
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• pp.115-120
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• 2010

모듈화된 부품을 이용하여 완성된 제품을 만드는 제품완성업체는 효율적인 부품공급시스템을 통해 부품공급업체로부터 적시에 조립라인에 부품을 공급받기를 원하며 이종부품에 대한 결품이 발생하지 않기를 기대한다. 스마트 파렛트 기반 부품공급시스템은 이러한 요구를 만족시키기 위하여 수작업에 의한 과다한 이종부품확인 시간을 소비하는 기존의 바코드 기반 방식의 단점과 RFID 기반 방식이 가진 전송거리 제한 문제를 해결하고자 제안되었다. 본 논문에서는 스마트 파렛트 기반 부품공급시스템이 가진 제한된 전력 문제를 보완하고 운영의 효율성을 극대화하기 위한 PDA 시스템을 제안하고 실제 구현하였다.

• Journal of Pharmaceutical Investigation
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• 제30권1호
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• pp.1-12
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• 2000

Gene therapy is a method for the treatment of diseases with introducing the gene-engineered materials into a patient with gene-deficiency disease (e.g. cystic fibrosis) or cancer to produce a therapeutic protein in a patient's cells. Successful gene therapy requires establishing both gene expression systems and delivery systems. Viral and non-viral vectors have been used for gene delivery. Viral vectors have a high transfection efficiency, but are limited in relations to issues of safety, toxicity and immunogenecity. Non-viral vectors are easy to prepare and relatively safe. However, non-viral vectors have a low transfection efficiency. Cationic liposomes are the most available among non-viral vectors. Cationic liposomes have been used to transfect cells both in vitro and in vivo experiments. Besides, several formulations containing cationic lipid are being used in clinical trials in cases of cystic fibrosis or cancer. A crucial subject to the further development of gene delivery vectors will be a long-term gene expression with following characteristics; protecting and deliverying DNA efficiently, non-toxic and non-immunogenic, and easy to produce in large scale.

• 산업공학
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• 제25권2호
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• pp.196-203
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• 2012

In the postal delivery service, both the standardization of business process and the workload measurement is very important part of saving the cost and improving the efficiency. However, these are not easy to apply to real situation because of the labor-intensive and diversification of delivery environment. In this study, we develop the methodology for the standardization of business process and workload measurement model of the postal delivery service. Firstly, we introduce the work factor analysis on the postal delivery process. Then, we propose the methodology workload measurement model based on diversification of delivery environment.

• 항공우주시스템공학회지
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• 제14권6호
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• pp.1-9
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• 2020

Global e-commerce and delivery companies are actively pursuing last-mile delivery service using drones, and various delivery schedule planning studies have been conducted. In this study, separate individual route networks were constructed to reflect drone route constraints such as prohibited airspace and truck route constraints such as rivers, which previous studies did not incorporate. The A* algorithm was used to calculate the shortest path distance matrix between the starting point and destinations. In addition, we proposed an optimal delivery schedule plan using genetic algorithms and applied it to compare the efficiency with that of vehicle-only delivery.

• Journal of Pharmaceutical Investigation
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• 제28권2호
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• pp.93-98
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• 1998

We have developed liposomes which can be easily prepared with inexpensive lipid, have enhanced stability, and can efficiently deliver DNA into the COS-l cells, Liposome formulations were prepared using cationic materials such as dimethyldioctadecyl ammonium bromide (DDAB), cetyltrimethyl ammonium bromide(CTAB), We investigated the effect of cationic liposome formulations on in vitro DNA transfection, DDAB-containing liposomes showed increased transfection efficiency which was 3.2-fold as much as that by $Lipofectin^{\circledR}$, but CTAB-containing liposomes were inactive in gene transfection. The effect of colipid of DDAB-containing liposome was also investegated. As a colipid, dioleylphosphatidylethanolamine(DOPE) and cholesterol did altered the transfection efficiency of DDAB-containing liposomes. And increased DDAB concentration lowered the transfection efficiency. The optimum amount of liposomal formulation was $10\;{\mu}M$ for $1\;{\mu}g$ of DNA. In the experiment of stability, DOPE-containing liposomes formulation showed a broad size distribution and separation of two major peaks on a 5th day of preparation, but liposomes containing cholesterol was stable for 10 days. DDAB-containing liposomal DNA delivery system was prepared easily and was stable.

• Macromolecular Research
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• 제17권1호
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• pp.19-25
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• 2009

In order to enhance the gene delivery efficiency and decrease the cytotoxicity of polyplexes, we synthesized Solutol-g-PEI by conjugating polyethyleneimine (PEI) to Solutol (polyoxyethylene (10) stearate), and evaluated its efficiency as a possible nonviral gene carrier candidate. Structural analysis of synthesized polymer was performed by using $^1H$-NMR. Gel retardation assay, particle sizes and zeta potential measurement confirmed that the new gene carrier formed a compact complex with plasmid DNA. The complexes were smaller than 150 nm, which implicated its potential for intracellular delivery. It showed lower cytotoxicity in three different cell lines (Hela, MCF-7, and HepG2) than PEI 25 kDa. pGL3-lus was used as a reporter gene, and the transfection efficiency was in vitro measured in Hela cells. Solutol-g-PEI showed much higher transfection efficiency than unmodified PEI 25 kDa.