• Journal of Veterinary Science
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• v.22 no.5
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• pp.63.1-63.14
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• 2021

Background: Recently, mesenchymal stem cells therapy has been performed in dogs, although the outcome is not always favorable. Objectives: To investigate the therapeutic efficacy of mesenchymal stem cells (MSCs) using dog leukocyte antigen (DLA) matching between the donor and recipient in vitro. Methods: Canine adipose-derived MSCs (cA-MSCs) isolated from the subcutaneous tissue of Dog 1 underwent characterization. For major DLA genotyping (DQA1, DQB1, and DRB1), peripheral blood mononuclear cells (PBMCs) from two dogs (Dogs 1 and 2) were analyzed by direct sequencing of polymerase chain reaction (PCR) products. The cA-MSCs were co-cultured at a 1:10 ratio with activated PBMCs (DLA matching or mismatching) for 3 days and analyzed for immunosuppressive (IDO, PTGS2, and PTGES), inflammatory (IL6 and IL10), and apoptotic genes (CASP8, BAX, TP53, and BCL2) by quantitative real-time reverse transcriptase-PCR. Results: cA-MSCs were expressed cell surface markers such as CD90⁺/44⁺/29⁺/45^- and differentiated into osteocytes, chondrocytes, and adipocytes in vitro. According to the Immuno Polymorphism Database, DLA genotyping comparisons of Dogs 1 and 2 revealed complete differences in genes DQA1, DQB1, and DRB1. In the co-culturing of cA-MSCs and PBMCs, DLA mismatch between the two cell types induced a significant increase in the expression of immunosuppressive (IDO/PTGS2) and apoptotic (CASP8/BAX) genes. Conclusions: The administration of cA-MSCs matching the recipient DLA type can alleviate the need to regulate excessive immunosuppressive responses associated with genes, such as IDO and PTGES. Furthermore, easy and reliable DLA genotyping technology is required because of the high degree of genetic polymorphisms of DQA1, DQB1, and DRB1 and the low readability of DLA 88.

• Journal of Acupuncture Research
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• v.36 no.1
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• pp.12-20
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• 2019

Background: This study was conducted to verify the effectiveness and effectiveness-related factors of Korean medicine (KM) treatment for patients who have been involved in traffic accidents (TA). Methods: The medical records of 150 TA patients hospitalized in Dongguk University Bundang Oriental Medicine Hospital from September 2017 to May 2018 were reviewed. Medical records included information on patient demographic characteristics, TA situations, use of KM treatment, clinical characteristics, 4 scales [numeric rating scale (NRS), visual analogue scale (VAS), EuroQol-visual analogue scale (EQ-VAS) and neck disability index (NDI)]. Statistical analyses were performed to determine the effectiveness and effectiveness-related factors of treatment. Results: The 150 patients (almost 1:1 gender ratio) were typically in their 40's (26.67%), had rear impact to their vehicle (37.33%), were in the driver's seat (70.00%), and without head trauma and loss of consciousness (83.33%). The main symptoms were neck pain (60.66%) and lower back pain (54.00%). All 150 patients showed significant improvement after KM treatment. 4 scales significantly improved when the treatment started within 3 days from the accident and patients were hospitalized longer and took more diverse treatments. The patients experiencing a higher initial pain level (NRS 8-10, $VAS{\geq}70$) showed significant improvement of NRS and VAS scores with faster and longer inpatient treatments than the other patients. The patients with higher initial pain levels ($VAS{\geq}70$) also showed significant improvement in EQ-VAS scores. Conclusion: KM treatment was effective for TA patients in this study. Treatment initiation time, hospitalization period, treatment diversity and initial pain level are related to the degree of improvement of a patient's symptoms.

• The Journal of Korean society of community based occupational therapy
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• v.8 no.3
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• pp.69-76
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• 2018

Objective : The study was to investigate the neurophysiological approach to the effect of complex exercise on memory, one of brain functions, and the degree of sleep disorder using experimental animals with sleep disorders. Methods : This study carried out a complex exercise that designed in an animal laboratory for 4 days to 16 sleep - disordered model rats. After the exercise, brain function was confirmed with the changes of BDNF in the hippocampus and the change of sleep level was confirmed with the concentration of melatonin in the blood. Results : First, the effect of the complex exercise program on brain function was significantly increased in the experimental group(p<0.01). Second, the effect of complex exercise program on sleep disturbance was significantly increased in the experimental group and control group(p<0.01)(p<0.05). Conclusion : The rate of increase of the elderly in the community is rapidly increasing, and the sleep disorder of the elderly can affect the quality of life of these elderly people. Secondary memory impairment due to sleep disturbances can also be a problem. Although there are many ways to improve sleep disturbance, it has been scientifically proven through experimental animals that sleep and memory can be improved with complex exercise that is not economically, spatially burdensome.

• Korean Journal of Agricultural and Forest Meteorology
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• v.20 no.4
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• pp.313-320
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• 2018

In the spring of 2018, opened-flowers of fruit trees were frozen to death due to abnormal low temperature around Jeonbuk Province and southern Gyeonggi Province areas. In the 2000s, abnormal weather is observed all over the world very frequently. As a consequence, various sectors of the society suffer from economic damage and negative effects of the abnormal weather. Moreover, recent global climate change is believed to increase the incidence of extreme weathers, which are out of the normal range of the local climate. It is necessary to identify these abnormal weather phenomena accurately and analyze the effects of them on crops in order to understand the effects of them on crop yields. This study projected the trend of the low-temperature occurrence in the future by predicting the changes in future flowering dates and quantifying the temperature distribution after flowering using climate change scenarios. This study targeted areas actually producing a major portion of pear, peach, and apple in South Korea. The results of this study predicted that the flowering dates of these fruits will be approximately 20 days earlier than the current normal year in the future (2071-2100) for the study area. Moreover, it was found that the distribution of low temperature would vary by fruit type and region to some degree. The results of this study present only a portion of fruit trees cultivars grown in South Korea. It was expected that, when this approach is applied to various crops and fruit trees, it will be possible to contribute to preparing countermeasures for climate change in the agricultural sector.

• Genes and Genomics
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• v.40 no.11
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• pp.1213-1223
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• 2018

Pelagic larval dispersal habits influence the population genetic structure of marine mollusk organisms via gene flow. The genetic information of the clam Gomphina aequilatera (short larval stage, 10 days) which is ecologically and economically important in the China coast is unknown. To determine the influence of planktonic larval duration on the genetic structure of G. aequilatera. Mitochondrial markers, cytochrome oxidase subunit i (COI) and 12S ribosomal RNA (12S rRNA), were used to investigate the population structure of wild G. aequilatera specimens from four China Sea coastal locations (Zhoushan, Nanji Island, Zhangpu and Beihai). Partial COI (685 bp) and 12S rRNA (350 bp) sequences were determined. High level and significant $F_{ST}$ values were obtained among the different localities, based on either COI ($F_{ST}=0.100-0.444$, P<0.05) or 12S rRNA ($F_{ST}=0.193-0.742$, P<0.05), indicating a high degree of genetic differentiation among the populations. The pairwise $N_m$ between Beihai and Zhoushan for COI was 0.626 and the other four pairwise $N_m$ values were >1, indicating extensive gene flow among them. The 12S rRNA showed the same pattern. AMOVA test results for COI and 12S rRNA indicated major genetic variation within the populations: 77.96% within and 22.04% among the populations for COI, 55.73% within and 44.27% among the populations for 12S rRNA. A median-joining network suggested obvious genetic differentiation between the Zhoushan and Beihai populations. This study revealed the extant population genetic structure of G. aequilatera and showed a strong population structure in a species with a short planktonic larval stage.

• Journal of the Korea Convergence Society
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• v.12 no.5
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• pp.139-145
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• 2021

Through this study, we intend to study user satisfaction in order to verify whether there is a need for full-scale research, development and commercialization of document viewers in a VR environment. VR content consists of realistic 3D graphics and 360-degree video, and provides a synesthesia experience and immersion. We developed and tested a VR document viewer prototype that can utilize this concept as a document viewing system. It can act as a viewer that provides an interactive viewing environment according to the user's body interaction and the direction of the field of view, and it can be said that the feature of VR document viewer is that it can draw the user's high level of immersion and concentration when using the viewer. The developed prototype was tested in a test group consisting of 100 VR experiences and device owners for about 1 hour and 3 days a day, and then a questionnaire survey in the form of a fixed selection question was conducted. This study is a prototype study of a document viewer suitable for a virtual reality environment, and can lead to a sense of immersion when reading a document, and suggest a new document viewer direction that is effective for visual fatigue and visual perception of the document.

• Korean Journal of Plant Taxonomy
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• v.50 no.4
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• pp.427-452
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• 2020

This study of vascular plants was conducted over a period of 33 days from March to October of 2018 in Byeonsanbando National Park, South Korea. Based on voucher specimens and photographs, 829 taxa in total were recorded, including 141 families, 458 genera, 748 species, 16 subspecies, 59 varieties, and 6 forma. Two endangered taxa were identified, Iris koreana Nakai and Saururus chinensis (Lour.) Baill. Five rare taxa were found, including Abeliophyllum distichum Nakai and Hypodematium glanduloso-pilosum (Tagawa) Ohwi. A total of 17 Korean endemic plants were investigated. Examples include Elsholtzia byeonsanensis M. Kim, Eranthis byunsanensis B. Y. Sun, and Lycoris flavescens M. Kim & S. T. Lee. Of floristic regional indicators (third to fifth degree), 52 taxa were identified. Naturalized plants were identified, which included 79 taxa, and 7 species of ecosystem disturbance plants, such as Ambrosia artemisiifolia L., Lactuca serriola L., and Solidago altissima L. were found. We recorded 39 taxa of the plants vulnerable to climate change.

• Korean Journal of Construction Engineering and Management
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• v.22 no.1
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• pp.81-89
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• 2021

In recent years, the design of buildings is changing from formal to creative and freeform. Accordingly, the scale of construction technology is changing to architectural design and construction of irregular buildings. Using the FDM method, which is one of the 3D printing technologies, it is possible to manufacture various forms of irregular formwork inexpensively and quickly coMPared to the existing formwork, and it seems to be able to solve the manpower problem. Using a 3D printer, the PLA filament formwork is produced in the form of a cylinder and a rectangular cuboid, and the usability of the PLA filament formwork is confirmed by examining the compression strength test and the degree of deformation and reusability over 28 days of age. Different sizes of additional specimens are also conducted according to the size. As a result of the experiment, it was confirmed that the filament formwork itself has about 3~4MPa strength. As a result of reviewing data through existing linear studies and experiments, it is appropriate to use more than 60% infill, and it is advantageous in terms of strength. As a result of cutting and dismantling the filament formwork, the surface is very clean and there is no damage, so it can be reused.

• Journal of the Korean Applied Science and Technology
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• v.38 no.1
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• pp.157-167
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• 2021

This study is a descriptive research study to find out the relationship between degree of Self-Esteem, Anger-Out, Anger-In, Anger-Control and SNS Addiction Tendency of high school students, and the factors that influence SNS addiction tendency. For a total of 10 days from May 18 to 28, 2020, a total of 100 people were collected using online questionnaires to those who were attending high schools in S and G regions and using SNS. As a result of the study, SNS addiction tendency and self-esteem(r=.385, p<.001), anger-control(r=-.354, p<.001) showed a normal inverse correlation, and an anger-out (r=.321, p=.001), anger-in (r=308, p=.002) showed a common net correlation. Factors affecting SNS addiction tendencies were self-esteem(β=-.297, p=.001), gender(β=.266, p=.003), and anger-out(β=.247, p=.007), with 27.7% explanatory power in the regression model(F=12.279, p<.001). Therefore, it is necessary to develop programs to increase high school students' self-esteem and lower their anger-out in order to reduce SNS addiction tendencies, especially for female high school students in gender.

• Korean Journal of Plant Resources
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• v.35 no.2
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• pp.248-288
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• 2022

The study on vascular plants of Gayasan National Park was conducted in 2016 over a period of 34 days from March to October 2016. As the study, a total of 768 taxa were recorded, including 118 families, 396 genera, 691 species, 15 subspecies, 51 varieties, and 11 forms. In the vascular plants, four endangered wild plants were recorded, Ponerorchis cucullata (L.) X.H.Jin, Trientalis europaea var. arctica (Fisch.) Ledeb., Cypripedium macranthos Sw., and Pedicularis hallaisanensis Hurus. Taxa of Least Concern (LC) or higher level on National Red List published by the Ministry of Environment of Korea were 21 taxa for example Tricyrtis macropoda Miq., and Lilium cernuum Kom. A total of 37 Korean endemic plants were investigated for example Abies koreana E.H.Wilson and Impatiens atrosanguinea (Nakai) B.U.Oh & Y.P.Hong. A total of 162 taxa of floristic regional indicators (3^rd to 5^th degree) were investigated including 9 taxa of V, 15 taxa of IV, 38 taxa of III, 45 taxa of II, and 53 taxa of I. Naturalized plants were recorded a total of 46 taxa with a Naturalization Index of 6.0%, the Urbanization Index was calculated to be 14.3% and 5 taxa of ecosystem disturbance plants were investigated such as Sicyos angulatus L., Rumex acetosella L., and Humulus scandens (Lour.) Merr. As the result of comparison with previous studies, in this study a total of 206 taxa were newly identified including 62 families, 144 genera, 184 species, 2 subspecies, 15 varieties, and 5 forms.