• 한국컴퓨터정보학회:학술대회논문집
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• 한국컴퓨터정보학회 2022년도 제65차 동계학술대회논문집 30권1호
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• pp.341-344
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• 2022

본 논문에서는 삼각형 메쉬 기반에서 변형률 기반 동역학(Strain-based dynamics, SBD)을 안정적으로 표현할 수 있는 굽힘 스프링 구조와 감쇠 기법에 대해 설명한다. SBD는 삼각형 메쉬의 에지 길이(Edge length) 기반의 에너지 대신 변형률(Strain)을 활용하여 에너지를 모델링한다. 하지만, 비정상적인 삼각형(Degenerate triangle)인 경우 변형률이 불안정하게 계산되어 잘못된 방향으로 늘어나는 문제가 발생한다. 본 논문에서는 이러한 문제를 효율적으로 처리할 수 있는 굽힘 스프링(Bending spring) 구조에 대해 소개한다. 결과적으로 본 논문에서 제안하는 기법은 안정적으로 SBD를 처리할 수 있기 때문에 다양한 재질의 옷감 시뮬레이션을 안정적으로 표현할 수 있도록 한다.

• 한국컴퓨터정보학회논문지
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• 제28권9호
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• pp.73-79
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• 2023

본 논문에서는 삼각형 메쉬(Triangular mesh) 기반에서 변형률 기반 동역학(Strain-based dynamics, SBD)을 안정적으로 표현할 수 있는 LOD(Level of detail)기반의 굽힘 스프링(Bending spring) 구조와 감쇠 기법에 대해 설명한다. SBD는 삼각형 메쉬의 에지 길이(Edge length) 기반의 에너지 대신 변형률(Strain)을 활용하여 탄성 에너지를 모델링한다. 하지만, 큰 외력이 발생하면 에지 기반으로 탄성 에너지를 계산하는 과정에서는 비정상적인 삼각형(Degenerate triangle)이 나타나고 이 문제는 불안정한 변형률을 계산하기 때문에 잘못된 방향으로 늘어나는 문제가 발생한다. 본 논문에서는 이 문제를 효율적으로 처리할 수 있는 LOD기반의 굽힘 스프링을 생성하고 에너지를 계산하는 방법에 대해 소개한다. 결과적으로 본 논문에서 제안하는 기법은 굽힘 스프링 기반의 SBD를 안정적이고 효율적으로 처리할 수 있기 때문에 옷감 시뮬레이션을 안정적으로 표현할 수 있다.

• Mycobiology
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• 제46권4호
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• pp.382-387
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• 2018

The entomopathogenic fungus Cordyceps militaris is a valuable medicinal ascomycete, which degenerates frequently during subsequent culture. To avoid economic losses during industrialized production, scratching stimuli of mycelia was introduced to improve the fruiting body production. The present results indicated that higher yields and biological efficiency were obtained from two degenerate strains (YN1-14 and YN2-7) but not from g38 (an insertional mutant in Rhf1 gene with higher yields and shorter growth periods). Furthermore, the growth periods of the fruiting bodies were at least 5 days earlier when the mycelia were scratched before stromata differentiation. Three ROS-scavenging genes including Cu/Zn superoxide dismutase (CmSod1), Glutathione peroxidase (CmGpx), and Catalase A (CmCat A) were isolated and their expression profiles against scratching were determined in degenerate strain YN1-14 and mutant strain g38. At day 5 after scratching, the expression level of CmGpx significantly decreased for strain g38, but that of CmSod1 significantly increased for YN1-14. These results indicated that scratching is an effective way to promote fruiting body production of degenerate strain, which may be related at least with Rhf1 and active oxygen scavenging genes.

• 콘크리트학회지
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• 제5권2호
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• pp.181-188
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• 1993

본 논문에서는 철근콘크리트 쉘 구조물의 크리프와 건조수축에 의한 시간의존성 효과를 포함하여 해석할 수 있는 기법을 개발하였다. Degenerate 쉘 요소를 해석에 사용하였으며 층 분할 기법을 이용하였다. 콘크리트의 압축 거동 모델은 탄-소성 모델 혹은 변형율 경화 모델을 사용할 수 있도록 하였고, 인장 영역에서는 균열 발생시 까지 선형 탄성으로 가정하였다. 철근은 등가의 두께를 가지는 철근 층으로 근사되었으며 각 철근 층은 철근의 배치 방향으로만 저항하는 일축거동을 하며 응력-변형율 곡선을 두 개의 직선으로 이상화 하였다. 비선형 해석을 위해 하중 증분 기법과 반복계산 기법을 사용하였으며 시간 의존성 효과를 고려하기 위해 시간영역을 같은 간격이 아닐 수도 있는 여러 개의 구간으로 나누어 해석하였다. 몇 개의 계산 예를 제시하고 다른 연구자들의 결과와 비교하여 본 연구의 타당성을 검토하였다.

• 한국미생물·생명공학회지
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• 제32권3호
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• pp.282-285
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• 2004

The polyene antibiotics including nystatin, pimaricin, amphotericin and candicidin are a family of most promising antifungal polyketide compounds, typically produced by rare actinomycetes species. The biosynthetic gene clusters for these polyenes have been previously investigated, revealing the presence of highly homologous biosynthetic genes among polyene-producers such as polyketide synthase (PKS) and cytochrome P450 hydroxylase (CYP) genes. Based on amino acid sequence alignment among actinomycetes CYP genes, the highly-conserved regions specific for only polyene CYP genes were identified and chosen for degenerate PCR primers, followed by the PCR-screening with various actinomycetes genomic DNAs. Among tested several polyene non-producing actinomycetes strains, Pseudonorcardia autotrophica strain was selected based on the presence of PCR product with polyene-specific CYP gene primers, and then confirmed to contain a cryptic novel polyene hydroxylase gene in the chromosome. These results suggest that the polyene-specific hydroxylase gene PCR should be an efficient way of screening and isolating potentially-valuable cryptic polyene antibiotic biosynthetic genes from various microorganisms including rare actinomycetes.

• Journal of Microbiology and Biotechnology
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• 제23권7호
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• pp.984-992
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• 2013

The entire nucleotide sequence of the xylose reductase (XR) gene in Candida milleri CBS8195 sourdough yeast was determined by degenerate polymerase chain reaction (PCR) and genome walking. The sequence analysis revealed an open-reading frame of 981 bp that encoded 326 amino acids with a predicted molecular mass of 36.7 kDa. The deduced amino acid sequence of XR of C. milleri was 64.7% homologous to that of Kluyveromyces lactis. The cloned XR gene was expressed in Saccharomyces cerevisiae, and the resulting recombinant S. cerevisiae strain produced xylitol from xylose, indicating that the C. milleri XR introduced into S. cerevisiae is functional. An enzymatic activity assay and semiquantitative reverse transcription-PCR revealed that the expression of CmXR was induced by xylose. The GenBank Accession No. for CmXR is KC599203.

• The Plant Pathology Journal
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• 제16권2호
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• pp.118-124
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• 2000

The coat protein (CP) gene of kyuri green mottle mosaic virus zucchini strain (KGMMV-Z) isolated from zucchini (Cucurbita pepo) in Chonfu, Korea in 1999 was sequenced by the reverse transcription and polymerase chain reaction with degenerate and generate primers originated from tobamoviruses. The degenerate primers were very effective in amplification of KGMMV-Z CP region. The KGMMV-Z CP gene consisted of 486 nucleotides and had the same nucleotide length compared with those of cucurbit-infecting tobamoviruses. KGMMV-Z CP gene shared 43.8, 44.2, and 44.4% nucleotide sequence similarity with the CP gene of cucumber green mottle mosaic virus watermelon strain (CGMMZ-W), CGMMV-KW1, and CGMMV-SH, respectively, whereas three CGMMV strains among themselves showed 98.6-99.6% nucleotide similarity. The deduced amino acids of KGMMV-Z CP gene were 161 amino acid residues with the molecular weight of 17,181 daltons. The first 24 codons of KGMMV-Z CP gene corresponded to the sequences of the N-terminal amino acid of the viral capsid protein. The amino acid sequences of KGMMV-Z CP had 45.3% similarity compared with those of three CGMMV strains. However, the amino acid sequences of CGMMV strains were identical. These results showed that two cucurbit-infecting tobamovirus members, KGMMV-Z and CGMMV were genetically distantly related.

• Structural Engineering and Mechanics
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• 제69권2호
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• pp.205-219
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• 2019

This paper analyzes nonlinear free vibration of the circular nano-tubes made of functionally graded materials in the framework of nonlocal strain gradient theory in conjunction with a refined higher order shear deformation beam model. The effective material properties of the tube related to the change of temperature are assumed to vary along the radius of tube based on the power law. The refined beam model is introduced which not only contains transverse shear deformation but also satisfies the stress boundary conditions where shear stress cancels each other out on the inner and outer surfaces. Moreover, it can degenerate the Euler beam model, the Timoshenko beam model and the Reddy beam model. By incorporating this model with Hamilton's principle, the nonlinear vibration equations are established. The equations, including a material length scale parameter as well as a nonlocal parameter, can describe the size-dependent in linear and nonlinear vibration of FGM nanotubes. Analytical solution is obtained by using a two-steps perturbation method. Several comparisons are performed to validate the present analysis. Eventually, the effects of various physical parameters on nonlinear and linear natural frequencies of FGM nanotubes are analyzed, such as inner radius, temperature, nonlocal parameter, strain gradient parameter, scale parameter ratio, slenderness ratio, volume indexes, different beam models.

• Mycobiology
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• 제42권1호
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• pp.46-51
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• 2014

A degenerated strain of Pleurotus eryngii KNR2312 was isolated from a commercial farm. Random amplified polymorphic DNA analysis performed on the genomic DNA of the normal and degenerated strains of this species revealed differences in the DNA banding pattern. A unique DNA fragment (1.7 kbp), which appeared only in the degenerated strain, was isolated and sequenced. Comparing this sequence with the KNR2312 genomic sequence showed that the sequence of the degenerated strain comprised three DNA regions that originated from nine distinct scaffolds of the genomic sequence, suggesting that chromosome-level changes had occurred in the degenerated strain. Using the unique sequence, three sets of PCR primers were designed that targeted the full length, the 5' half, and the 3' half of the DNA. The primer sets P2-1 and P2-2 yielded 1.76 and 0.97 kbp PCR products, respectively, only in the case of the degenerated strain, whereas P2-3 generated a 0.8 kbp product in both the normal and the degenerated strains because its target region was intact in the normal strain as well. In the case of the P2-1 and P2-2 sets, the priming regions of the forward and reverse primers were located at distinct genomic scaffolds in the normal strain. These two primer sets specifically detected the degenerate strain of KNR2312 isolated from various mushrooms including 10 different strains of P. eryngii, four strains of P. ostreatus, and 11 other wild mushrooms.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권5호
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• pp.432-443
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• 2005

Microarray technology has contributed Significantly to the understanding of bacterial genetics and transcriptional regulation. One neglected aspect of this technology has been optimization of microarray-generated signals and quality of generated information. Full genome microarrays were developed for Clostridium acetobutylicum through spotting of PCR products that were designed with minimal homology with all other genes within the genome. Using statistical analyses it is demonstrated that Signal quality is significantly improved by increasing the hybridization volume. possibly increasing the effective number of transcripts available to bind to a given spot, while changes in labeled probe amounts were found to be less sensitive to improving signal quality. In addition to Q-RT-PCR, array validation was tested by examining the transcriptional program of a mutant (M5) strain lacking the pSOL1 178-gene megaplasmid relative to the wildtype (WT) strain. Under optimal conditions, it is demonstrated that the fraction of false positive genes is 1% when considering differentially expressed genes and 7% when considering all genes with signal above background. To enhance genomic-scale understanding of organismal physiology, using data from these microarrays we estimated that $40{\sim}55%$ of the C. acetobutylicum genome is expressed at any time during batch culture, similar to estimates made for Bacillus subtilis.