• Title/Summary/Keyword: defect factor

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THE EXPERIMENTAL STUDY FOR INFLUENCE OF FIBRIN GLUE DURING HEALING PHASE AFTER MAKING ARTIFICIAL BONE DEFECT (백서 두개골 결손부의 골재생에 fibrin glue가 미치는 영향에 관한 실험적 연구)

  • Rim, Jae-Suk;Jang, Hyon-Seok;Eune, Jung-Ju;Lee, Eui-Seok;Park, Eun-Yong;Suh, Je-Duck
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.27 no.5
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    • pp.431-437
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    • 2005
  • Fibrin glue is composed of fibrinogen and thrombin and used in various regions for multiple use. Basic principle is that thrombin converts fibrinogen to fibrin in the presence of $Ca^{2+}$. The structure of fibrin is loose at the beginning, but after about 5 minutes a tight structure is formed under the influence of factor VIII which changes fibrin monomer into fibrin polymer. Fibrin glue is used for tissue adhesive, suture, local hemostasis, wound healing, closure of subdural space. Fibrin adhesive has been used in oral and maxillofacial surgery for hemostasis after tooth extraction in patients with coagulation disorders, skin graft fixation, reattachment of periodontal flaps, in combination with autogenous bone chips to fill the bony cavities following cyst removal, and for securing the hydroxyapatite granules for maxillary alveolar ridge augmentation. This study was designed for researching influence of fibrin glue during healing phase after making artificial bone defect.

Combined effect of bisphosphonate and recombinant human bone morphogenetic protein 2 on bone healing of rat calvarial defects

  • Kim, Ho-Chul;Song, Jae-Min;Kim, Chang-Joo;Yoon, Sang-Yong;Kim, In-Ryoung;Park, Bong-Soo;Shin, Sang-Hun
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.37
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    • pp.16.1-16.7
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    • 2015
  • Background: This study aimed to investigate new bone formation using recombinant human bone morphogenetic protein 2 (rhBMP-2) and locally applied bisphosphonate in rat calvarial defects. Methods: Thirty-six rats were studied. Two circular 5 mm diameter bony defect were formed in the calvaria using a trephine bur. The bony defect were grafted with $Bio-Oss^{(R)}$ only (group 1, n = 9), $Bio-Oss^{(R)}$ wetted with rhBMP-2 (group 2, n = 9), $Bio-Oss^{(R)}$ wetted with rhBMP-2 and 1 mM alendronate (group 3, n = 9) and $Bio-Oss^{(R)}$ wetted with rhBMP-2 and 10 mM alendronate (group 4, n = 9). In each group, three animals were euthanized at 2, 4 and 8 weeks after surgery, respectively. The specimens were then analyzed by histology, histomorphometry and immunohistochemistry analysis. Results: There were significant decrease of bone formation area (p < 0.05) between group 4 and group 2, 3. Group 3 showed increase of new bone formation compared to group 2. In immunohistochemistry, collagen type I and osteoprotegerin (OPG) didn't show any difference. However, receptor activator of nuclear factor ${\kappa}B$ ligand (RANKL) decreased with time dependent except group 4. Conclusion: Low concentration bisphosphonate and rhBMP-2 have synergic effect on bone regeneration and this is result from the decreased activity of RANKL of osteoblast.

Risk factors of ocular involvement in children with mitochondrial respiratory chain complex defect

  • Chae, Jung-Hyun;Lee, Jung-Hun;Kim, Kyo-Ryung;Byeon, Suk-Ho;Lee, Young-Mock;Kang, Hoon-Chul;Lee, Joon-Soo;Kim, Heung-Dong
    • Clinical and Experimental Pediatrics
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    • v.53 no.12
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    • pp.994-999
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    • 2010
  • Purpose: Mitochondrial dysfunction can present with various symptoms depending on the organ it has affected. This research tried to analyze the ophthalmologic symptoms and ophthalmologic examination (OE) results in patients with mitochondrial disease (MD). Methods: Seventy-four patients diagnosed with mitochondrial respiratory chain complex defect with biochemical enzyme assay were included in the study. They were divided into 2 groups based on the OE results by funduscopy and were analyzed on the basis of their clinical features, biochemical test results, morphological analysis, and neuroimaging findings. Results: Thirty-seven (50%) of the 74 MD patients developed ophthalmologic symptoms. Abnormal findings were observed in 36 (48.6%) patients during an OE, and 16 (21.6%) of them had no ocular symptoms. Significantly higher rates of prematurity, clinical history of epilepsy or frequent apnea events, abnormal light microscopic findings in muscle pathology, diffuse cerebral atrophy in magnetic resonance imaging, and brainstem hyperintensity and lactate peaks in magnetic resonance spectroscopy were noted in the group with abnormal OE results. Conclusion: Although the ophthalmologic symptoms are not very remarkable in MD patients, an OE is required. When the risk factors mentioned above are observed, a more active approach should be taken in the OE because a higher frequency of ocular involvement can be expected.

The Effects of Current Types on Through Via Hole Filling for 3D-SiP Application (전류인가 방법이 3D-SiP용 Through Via Hole의 Filling에 미치는 영향)

  • Chang, Gun-Ho;Lee, Jae-Ho
    • Journal of the Microelectronics and Packaging Society
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    • v.13 no.4
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    • pp.45-50
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    • 2006
  • Copper via filling is the important factor in 3-D stacking interconnection of SiP (system in package). As the packaging density is getting higher, the size of via is getting smaller. When DC electroplating is applied, a defect-free hole cannot be obtained in a small size via hole. To prevent the defects in holes, pulse and pulse reverse current was applied in copper via filling. The holes, $20\and\;50{\mu}m$ in diameter and $100{\sim}190\;{\mu}m$ in height. The holes were prepared by DRIE method. Ta was sputtered for copper diffusion barrier followed by copper seed layer IMP sputtering. Via specimen were filled by DC, pulse and pulse-reverse current electroplating methods. The effects of additives and current types on copper deposits were investigated. Vertical and horizontal cross section of via were observed by SEM to find the defects in via. When pulse-reverse electroplating method was used, defect free via were successfully obtained.

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Eight-week healing of grafted calvarial bone defects with hyperbaric oxygen therapy in rats

  • Oh, Seo-Eun;Hu, Kyung-Seok;Kim, Sungtae
    • Journal of Periodontal and Implant Science
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    • v.49 no.4
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    • pp.228-236
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    • 2019
  • Purpose: The purpose of this study was to evaluate the synergistic effect of adjunctive hyperbaric oxygen (HBO) therapy on new bone formation and angiogenesis after 8 weeks of healing. Methods: Sprague-Dawley rats (n=28) were split into 2 groups according to the application of adjunctive HBO therapy: a group that received HBO therapy (HBO group [n=14]) and another group that did not receive HBO therapy (NHBO group [n=14]). Each group was divided into 2 subgroups according to the type of bone graft material: a biphasic calcium phosphate (BCP) subgroup and an Escherichia coli-derived recombinant human bone morphogenetic protein-2-/epigallocatechin-3-gallate-coated BCP (mBCP) subgroup. Two identical circular defects with a 6-mm diameter were made in the right and left parietal bones of each rat. One defect was grafted with bone graft material (BCP or mBCP). The other defect was not grafted. The HBO group received 2 weeks of adjunctive HBO therapy (1 hour, 5 times a week). The rats were euthanized 8 weeks after surgery. The specimens were prepared for histologic analysis. Results: New bone (%) was higher in the NHBO-mBCP group than in the NHBO-BCP and control groups (P<0.05). Blood vessel count (%) and vascular endothelial growth factor staining (%) were higher in the HBO-mBCP group than in the NHBO-mBCP group (P<0.05). Conclusions: HBO therapy did not have a positive influence on bone formation irrespective of the type of bone graft material applied after 8 weeks of healing. HBO therapy had a positive effect on angiogenic activity.

Determination of the optimal diabetes duration for bone regeneration experiments in an alloxan-induced diabetic rabbit calvarial defect model

  • Jeong, Sang-Hun;Jung, Bo Hyun;Yoo, Ki-Yeon;Um, Heung-Sik;Chang, Beom-Seok;Lee, Jae-Kwan;Choi, Won-Youl
    • Journal of Periodontal and Implant Science
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    • v.48 no.6
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    • pp.383-394
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    • 2018
  • Purpose: The purpose of this study was to evaluate the optimal diabetes duration for bone regeneration experiments in an alloxan monohydrate (ALX)-induced diabetic rabbit calvarial defect model by evaluating the association between diabetes duration and bone healing capacity. Methods: Twenty-four New Zealand white rabbits were used. Twenty-two rabbits were injected with 100 mg/kg of ALX to induce experimental diabetes. These rabbits were divided into 4 groups, including a control group and groups with diabetes durations of 1 week (group 1), 2 weeks (group 2), and 4 weeks (group 3). Calvarial defects were created at 1, 2, and 4 weeks after ALX injection and in the control rabbits. Cone-beam computed tomography (CBCT) scanning was performed on the day of surgery and at 2 and 4 weeks after surgery. The rabbits were sacrificed 4 weeks after surgery, followed by histological and immunofluorescence analysis. Results: The diabetic state of all diabetic rabbits was well-maintained throughout the experiment. Reconstructed 3-dimensional CBCT imaging showed more rapid and prominent bone regeneration in the control group than in the experimental groups. Histological staining showed notable bone regeneration in the control group, in contrast to scarce bone formation in the experimental groups. The appearance and immunoreactivity of receptor activator of nuclear factor-kappa B and osteoprotegerin did not show notable differences among the groups. Conclusion: ALX administration at 100 mg/kg successfully induced experimental diabetes in rabbits. The effect of diabetes on bone healing was evident when the interval between diabetes induction and the intervention was ${\geq}1$ week.

Development of Durable Reliability Assessment Methods for Heavy Duty Coatings

  • Kim, Seung-Jin;Jung, Ho;Yang, In-Mo;Tanaka, Takeyuki
    • Corrosion Science and Technology
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    • v.4 no.4
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    • pp.155-163
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    • 2005
  • Heavy duty coating are required to have minimum durable period of 15 years under average usage environment because these paints are coated with purpose of anti-corrosion, antifouling, plastering etc. Onto steel structures constructed upon land and sea and other ferrous structures of electric power generation plants, electricity transmission towers, large structures of various plants, etc. Therefore we tried to estimate heavy duty coating longevity through reliability evaluation method and used combined cyclic anti-conrrosion test method composed of drying, moisturizing and salt spray as for accelerated life test to estimate longevity. Accelerated life test hours to heavy duty coating of first grade (with longevity not less than 15 years) specification may be obtained from troubleless test hours $t_n=\frac{B_p}{n^{1/\beta}}\left[\frac{1n(1-CL)}{1n(1-p)} \right]^{1/\beta}=19.671$ (yr) where shape parameter $\beta=1.1$, confidence level CL=80 %, warranty life $B_{10}=15$ yr and sampling size n=10 (2 sets). Because acceleration factor {AF} found by accelerated test is 41.7, accelerated life test hours required may be represented about 4,132 hr so that if this amount of hours is converted to number of cycles(6 hr/cycle) of complex cycle corrosion resistance test then the amount is tantamount about 690 cycles. That means if there does not occur trouble failure (with defect factor sum not more than 20) during when there is performed 690 cycles of combined cyclic anti-corrosion test to heavy duty coating specimen then it signifies that there can be warranted longevity $B_{10}$ of 15 yr under condition of confidence level CL=80 %.

Effectiveness of Autogenous Tooth Bone Graft Combined with Growth Factor: Prospective Cohort Study

  • Ahn, Kyo-Jin;Kim, Young-Kyun;Yun, Pil-Young;Lee, Bu-Kyu
    • Journal of Korean Dental Science
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    • v.6 no.2
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    • pp.50-57
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    • 2013
  • Purpose: Autogenous tooth bone graft is proven to be efficient. We evaluated the bone healing effect and clinical capabilities of autogenous tooth bone materials as a scaffold when growth factor is used together with this material. Materials and Methods: Subjects were those who needed implant placement and bone graft because of missing tooth or alveolar bone defect and who kept their autogenous tooth or needed extraction of other tooth. Group I included autogenous tooth bone graft with growth factor, whereas Group II had only autogenous tooth bone graft. We investigated the bone healing state through computed tomography taken just before surgery and 3 to 4 months after surgery to evaluate the effectiveness of bone graft. Group I had 9 patients, whereas Group II had 5 patients. We compared the pre- and post-operative increase of the Hounsfield unit and bone height by analyzing the computed tomography images. Result: Sinus bone grafts numbered 8 cases, and vertical ridge augmentation was performed together with 3 cases of these. Vertical ridge augmentation was performed in 2 cases, and horizontal ridge augmentation in 1 case alone. Socket graft was done in 3 cases. The post-operative mean value of the Hounsfield unit was 960 in Group I and 836.7 in Group II, but the increase was almost similar, i.e., 636.9 in Group I and 634.7 in Group II on the average. Increase of bone height was 7.6 mm in Group I and 11.1 mm in Group II on the average. This difference was attributable to the fact that most of the cases were sinus bone graft in Group II. Conclusion: In this study, we suggest the possibility of autogenous tooth bone graft materials as a scaffold besides their bone healing ability.

Respiratory Severity Score as a Predictive Factor for the Mortality of Congenital Diaphragmatic Hernia

  • Ahn, Ja-Hye;Jung, Young Hwa;Shin, Seung Han;Kim, Hyun-Young;Kim, Ee-Kyung;Kim, Han-Suk
    • Neonatal Medicine
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    • v.25 no.3
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    • pp.102-108
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    • 2018
  • Purpose: Congenital diaphragmatic hernia (CDH) is rare but potentially fatal. The overall outcome is highly variable. This study aimed to identify a simple and dynamic parameter that helps predict the mortality of CDH patients in real time, without invasive tests. Methods: We conducted a retrospective chart review of 59 CDH cases. Maternal and fetal information included the gestational age at diagnosis, site of defect, presence of liver herniation, and lung-to-head ratio (LHR) at 20 to 29 weeks of gestational age. Information regarding postnatal treatment, including the number of days until surgery, the need for inhaled nitric oxide (iNO), the need for extracorporeal membrane oxygenation (ECMO), and survival, was collected. The highest respiratory severity score (RSS) within 24 hours after birth was also calculated. Results: Statistical analysis showed that a younger gestational age at the initial diagnosis (P<0.001), a lower LHR (P=0.001), and the presence of liver herniation (P=0.003) were prenatal risk factors for CDH mortality. The RSS and use of iNO and ECMO were significant factors affecting survival. In the multivariate analysis, the only remaining significant risk factor was the highest preoperative RSS within 24 hours after birth (P=0.002). The area under the receiver operating characteristic curve was 0.9375, with a sensitivity of 91.67% and specificity of 83.87% at the RSS cut-off value of 5.2. The positive and negative predictive values were 82.14% and 92.86%, respectively. Conclusion: Using the RSS as a prognostic predictor with simple calculations will help clinicians plan CDH management.

Effect of Stress Ratio on Fatigue Fracture of a Shot Peening Marine Structural Steel (쇼트피닝 가공된 해양구조용강의 피로파괴에 미치는 응력비의 영향)

  • PARK KYOUNG-DONG;JIN YOUNG-BEOM;PARK HYOUNG-DONG
    • Journal of Ocean Engineering and Technology
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    • v.18 no.5
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    • pp.43-49
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    • 2004
  • The lightness of components required in the automobile and machine industry necessitates the use of high strength components. In particular, the fatigue failure phenomena, which occurs when using metal, increases the danger to human life and property. Therefore, antifatigue failure technology is an integral part of current industries. Currently, the shot peening is used for removing the defect from the surface of steel, while improving the fatigue strength on surface. Therefore, in this paper, the effect of compressive residual stress of spring steel(JISG SUP-9) by shot-peening on fatigue crack growth characteristics in a stress ratio(R=0.1, R=0.3, R=0.6) was investigated, giving consideration to fracture mechanics. By using the methods mentioned above, following conclusions are drawn: (1) The fatigue crack growth rate(da/dN) of the shot-peening material was lower than that of the un-peening material and in stage I, ΔKth, the threshold stress intensity factor of the shot-peen processed material is high in critical parts, unlike the un-peening material. Also m, fatigue crack growth exponent and number of cycle of the shot-peening material, was higher than that of the un-peening material, as concluded from effect of da/dN. (2) Fatigue life shows more improvement in the shot-peening material than in the un-peening material, and the compressive residual stress of surface on the shot-peen processed operate resistance of fatigue crack propagation.