• Journal of Korean Society of Water and Wastewater
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• v.23 no.2
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• pp.199-205
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• 2009

It is very important to differentiate of DNA derived from live or dead bacteria within mixed microbial communities in aquatic environments. Ethidium monoazide (EMA) is a DNA intercalating agent and the treatment of EMA with strong visible light cleaves the genomic DNA of bacteria. In dead bacterial cells, EMA intercalates into the genomic DNA, induces the cleavage of DNA, and inhibits the PCR amplification. In this study, we developed the EMA-PCR and EMA real-time PCR to detect the DNA derived from viable Escherichia coli (E.coli) in mixed cultures of live and dead E.coli. The treatment of EMA, $50{\mu}g/mL$, and 650 W visible halogen light exposure for 2 minutes cleaved the genomic DNA derived from heat killed E.coli but did not those of live E.coli. EMA-PCR could detect the DNA from live E.coli in mixed culture samples of live and dead E.coli at various ratio and there was no DNA amplification in only dead E.coli cultures. Similar results were observed in EMA real-time PCR. Further studies are needed to develop various EMA-PCR methods to detect viable waterborne pathogens such as Helicobacter pylori, Giardia lamblia, and so on.

• Journal of The Korean Society of Grassland and Forage Science
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• v.7 no.2
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• pp.87-91
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• 1987

This study was carried out to investigate the effects of different cutting times at 0, 10 and 20 days after lodging on the percentage of dead plants and bare ground, live and dead matter build-up, and botanical composition of orchardgrass dominated pasture during the mid summer season. For the test, a randomized block design with 3 replications was treated with 3 different cutting times (July 17, July 27 and Aug. 6). The results obtained are summarized as follows: 1. The percentage of dead plants with cutting times at 0, 10 and 20 days after lodging was 26.3,41.7 and 46.7'%, respectively. 2. The percentage of bare ground with cutting times at 0, 10 and 20 days after lodging was 13.3,26.7 and 3 1.7%, respectively. 3. Dead matter build-up with cutting times at 0, 10 and 20 days after lodging was increased significantly with later cutting (p<0.05). The ratio of live and dead matter build-up with cutting times at 0, 10 and 20 days after lodging was 90.2:9.8, 47.7:52.3 and 39.1:60.9%, respectively. 4. Regrowth yield after lodging was decreased significantly (p<0.05) with later cutting time. 5. The percentage of legumes in botanical composition was increased greatly with later cutting time. The ratio of grasses and legumes with cutting times at 0, 10 and 20 days after lodging was 77.8:21.6,45.5: 50.3 and 36.5:61.7%, respectively.

• Membrane Journal
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• v.9 no.1
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• pp.36-42
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• 1999

Permeation behavior of the semiconductor rinsing wastewater contammg Si particles was examined by ultrafiltration using the polyolefin tubular membrane. Flux decline with time was due to the growth of Si cake deposited on the membrane surface and the pore plugging by Si particles. Cake filtration from the cross flow application is compared to the combination of pore blocking and cake filtration from the dead-end application. The cake resistance is 3.16 x $10^{12}$ -4.34 X $\times$$10^{12}$ $m^{-1}$ for the cross flow and 6.6 x $\times$$10^{12}$ -12.19 X $\times$$10^{12}$ $\times$$m^{-1}$for the dead-end flow, respectively. At the initial stage of operation, permeation flux of cross flow type was 1.7 time higher than that of the dead end flow type. Permeation flux of cross flow was about 42 e 1m2 hr and the rejection rate of Si particles was about 96 %. The average particle size of Si particle in the permeate was 20 nm.

• 제어로봇시스템학회:학술대회논문집
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• 1993.10a
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• pp.365-369
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• 1993

In this paper, the new M/T method for motor speed detection is proposed. This method can be able to reduce the dead time compared with it when Ohmae's M/T method is implemented. And the comparsion of the dead time length between the Ohmae's method and the proposed method is analyzed quantitatively. Actually we implemented the new proposed M/T method using the hardware and software and verified the effectiveness of the proposed M/T method.

• Proceedings of the KIEE Conference
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• 1994.07a
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• pp.262-264
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• 1994

In is paper, a novel dead time compensation method is presented which produces inverter output voltages equal to reference voltages. An experimental result is also presented to demonstrate the validity of the proposed method. The reference voltage can be used as a feedback value, which is essential for sensorless vector control and flux estimation. The method can be carried out automatically by an inverter controller for initial set-up without any extra hardware.

• Proceedings of the KIEE Conference
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• 1997.07b
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• pp.612-615
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• 1997

In this paper, a robust speed controller considering the effect of uncertainty (plant parameter variation. external load disturbance. unmodeled and nonlinear dynamics etc..) for induction motor is proposed. Firstly. the dynamic model at nominal case of induction motor is estimated. Based on the estimated model. the IPSC ( Integral - Proportional Speed Controller) is designed. Then a DTRC (Dead-time Robust Controller) combining DTC ( Dead-time Compensator) & SRC (Simple Robust Controller) is designed to reduce the effects of parameter variation and external disturbance. Some simulated results are provided to demonstrate the effectiveness of the proposed controller.

• Proceedings of the KIEE Conference
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• 1995.11a
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• pp.131-136
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• 1995

This paper presents a functional ability improvement of auto-reclosing relay in the power transmission line protection. When the high speed auto-reclosing is successful, Auto-reclosing is practically valuable to improve the transient stability limit of a power system, but it is fail due to surviving fault, both electrical and mechanical stresses can result on the transformers and turbine-generator. It is true that the longer dead time of the reclosing relay gives the higher rate of successful reclosing, On the other hand, the power system does not always need high speed reclosing because of enough stability margin. This paper proposed "stability margin based dead time reclosing" in order to decrease not only the rate of unsuccessful reclosing, but the possibility of the harmful stress also. On-line transient stability assessment using artificial neural network, for implementing the proposed scheme, has studied and tested with resonable results.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.1
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• pp.187-192
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• 2009

This paper presents a method for designing a robust controller that alleviates disturbance effects and compensates performance degradation owing to the time-delay. Disturbance observer(DOB) approach as a tool of robust control has been widely employed in industry. However, since the Pade approximation of time-delay makes the plant non-minimum phase, the classical DOB cannot be applied directly to the system with time-delay. By using a new DOB structure for non-minimum phase systems together with the Smith Predictor, we propose a new controller for reducing the both effects of disturbance and time-delay. Moreover, the closed-loop system can be made robust against uncertain time-delay with the help of a Pill controller tuning method that is based on a second-order plus dead time modeling technique.

• Journal of Advanced Navigation Technology
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• v.19 no.6
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• pp.636-642
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• 2015

This paper presents an application of heuristic harmony search (HS) optimization algorithm for the identification of linear continuous time-delay system from step response. Identification model is first-order plus dead time (FOPDT), which describes a linear monotonic process quite well in most chemical processes and HAVC process and is often sufficient for PID controller tuning. This recently developed HS algorithm is conceptualized using the musical process of searching for a perfect state of harmony. It uses a stochastic random search instead of a gradient search so that derivative information is unnecessary. The effectiveness of the identification method has been demonstrated through a number of simulation examples.

• Journal of Power Electronics
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• v.16 no.5
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• pp.1661-1668
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• 2016

A fixed off-time controlled high power factor ac-dc LED driver is proposed in this paper, which employs a novel zero-crossing-compensation (ZCC) circuit based on a fixed off-time controlled scheme. Due to the parasitic parameters of the system, the practical waveforms have a dead region. By detecting the zero-crossing boundary, the proposed ZCC circuit compensates the control signal VCOMP within the dead region, and is invalid above this region. With further optimization of the parameters KR and Kτ of the ZCC circuit, the dead zone can be eliminated and lower THD is achieved. Finally, the chip is implemented in HHNEC 0.5μm 5V/40V HVCMOS process, and a prototype circuit, delivering 7~12W of power to several 3-W LED loads, is tested under AC input voltage ranging from 85V to 265V. The test results indicate that the average total harmonic distortion (THD) of the entire system is approximately 10%, with a minimum of 5.5%, and that the power factor is above 0.955, with a maximum of 0.999.