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Hatching Rate of Eggs, and Growth of Larvae and Juveniles from Selected Olive Flounder, Paralichthys olivaceus (선발 육종된 넙치, Paralichthys olivaceus의 부화율 및 자치어 성장)

  • Min, Byung-Hwa;Lee, Jeong-Ho;Noh, Jae-Koo;Kim, Hyun-Chul;Park, Choul-Ji;Choi, Sang-Jun;Myeong, Jeong-In
    • Development and Reproduction
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    • v.13 no.4
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    • pp.239-247
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    • 2009
  • Hatching rate, larval deformation and growth rate of selected olive flounder (Paralichthys olivaceus) for rapid growth were compared to those of the unselected fish. Fish were spawned on the same day and cultured under the similar conditions. The selected fish had a significantly higher eggs hatching rate, and lower larval deformation. The selected fish grew significantly faster, and at the end of the experiment (8 weeks after hatching) averaged 50.49${\pm}$2.67 mm in total length, 16.30${\pm}$0.08 mm in body height, and 1.036${\pm}$0.118 g in weight, compared to 40.55${\pm}$3.13 mm, 13.50${\pm}$0.96 mm, and 0.557${\pm}$0.073 g for unselected fish, respectively. The selected fish had a significantly higher body shape index, however lower condition factor than the unselected fish. The results of the present study demonstrate that the selected fish of the olive flounder for rapid growth had superior growth and improved body shape.

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Thermal and Uplift Histories of the Jurassic Granite Batholith in Southern Jeonju: Fission-track Thermochronological Analyses (전주 남부지역 쥬라기 화강암질 저반체의 지열사와 융기사: 피션트랙 열연대학적 해석)

  • Shin, Seong-Cheon
    • Economic and Environmental Geology
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    • v.49 no.5
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    • pp.389-410
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    • 2016
  • Wide ranges of fission-track (FT) ages were obtained from the Jurassic granite batholith in Jeonju-Gimje-Jeongeup area, southwestern Okcheon Belt: sphene=158~70 Ma; zircon=127~71 Ma; apatite=72~46 Ma. Thermochronological analyses based on undisturbed primary cooling and reset or partially-reduced FT ages, and some track-length data reveal complicated thermal histories of the granite. The overall cooling of the batholith is characterized by a relatively rapid earlier-cooling (${\sim}20^{\circ}/Ma$) to $300^{\circ}C$ isotherm since its crystallization and a very slow later-cooling ($2.0{\sim}1.5^{\circ}/Ma$) through the $300^{\circ}C-200^{\circ}C-100^{\circ}C$ isotherms to the present surface temperature. It is indicated that the large part of Jurassic granitic body experienced different level of elevated temperatures at least above $170^{\circ}C$ (maximum>$330^{\circ}C$) by a series of igneous activities in late Cretaceous. Consistent FT zircon ages from duplicate measurements for two sites of later igneous bodies define their formation ages: e.g., quartz porphyry=$73{\pm}3Ma$; diorite=$73{\pm}2Ma$; rhyolite=$72{\pm}3Ma$; feldspar porphyry=$78{\pm}4Ma$ (total weighted average=$73{\pm}3Ma$). Intrusions of these later igneous bodies and pegmatitic dyke swarms might play important roles in later thermal rise over the study area including hot-spring districts (e.g., Hwasim, Jukrim, Mogyokri, Hoebong etc.). On the basis of an assumption that the latercooling of granite batholith was essentially controlled by the denudation of overlying crust, the uplift since early Cretaceous was very slow with a mean rate of ~0.05 mm/year (i.e., ~50 m/Ma). Estimates of total uplifts since 100 Ma, 70 Ma and 40 Ma to present-day are ~5 km, ~3.5 km and ~2 km, respectively. The consistent values of total uplifts from different locations may suggest a regional plateau uplift with a uniform rate over the whole granitic body.

Development of New Mushroom Substrate of Pleurotus Ostreatus Using Foodwaste and Wormcast (남은 음식물과 지렁이 분변토를 이용한 느타리버섯의 대체 배지개발)

  • Park, Jang-Woo;Park, Ki-Byung;Lee, Eun Young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.20 no.4
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    • pp.75-85
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    • 2012
  • This study was investigated to develop an alternative medium for the cultivation of oyster mushrooms. The cultivation characteristics for major cultivated varieties of oyster mushrooms were campared with the food waste medium. In addition, water control & a gap formation of food waste is being pointed out as a problem of the medium material. Therefore, certain amounts of earthworm casting were supplied to the food waste medium to overcome the water and compaction phenomenon. This study investigated the use of the total 6 cultivars of oyster mushrooms, the Suhan(PL.1), Chiak No. 5(PL.2), Chiak No. 7(PL.3), Samgu No. 5(PL.4), Samgu No. 8(PL.5), Jangan No. 5(PL.6). In terms of medium, the optimum growth of the mycelium of the oyster mushroom was observed in the beet pulp medium but in food waste, the mycelium's growth was observed as insufficient in the bulk of cultivars. The Jangan No. 5(PL.6) was superior to other cultivars relative to the mycelium's growth. The optimum concentration of earthworm casting added the foodwaste medium was 30%. When Jangan No. 5(PL.6) was incubated in the foodwaste meidum containing 30% earthworm casting, the early days to germination were long, almost double the amount of time than that of the beet pulp medium. However, it was found that the fruit body size was larger than that of the beet pulp by up to 20.5%, and the length of the fruit body was up to 6.2% longer than that of the beet plup. The total yields increased by 60.5%. The analysis results of the oyster mushroom's nutritional contents cultured using the food waste medium, shows that the protein, vitamin A and vitamin E increased 70.6%, 2.4%, 0.8% respectively, the fat decreased by 12.6% and the oleic acid increased in a very small amount compared to that of the beet pulp medium.

Estimation of Optimum Period for Spring Cultivation of 'Chunkwang' Chinese Cabbage Based on Growing Degree Days in Korea (생육도일(GDDs)에 따른 '춘광' 봄배추의 적정 재배 작기 예측)

  • Wi, Seung Hwan;Song, Eun Young;Oh, Soon Ja;Son, In Chang;Lee, Sang Gyu;Lee, Hee Ju;Mun, Boheum;Cho, Young Yeol
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.20 no.2
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    • pp.175-182
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    • 2018
  • Knowledge of the optimum cultivation period for Chinese cabbage would help growers especially in spring in Korea. Growth and yield of Chinese cabbage in a temperature gradient chamber was evaluated for the growing periods of 64 days from three set of transplanting dates including March 6, March 20, and April 3 in 2017. Air temperature in the chamber was elevated step-by-step, by $2^{\circ}C$ above the ambient temperature. This increment was divided into three phases; i.e. low (ambient+$2^{\circ}C$, A), medium (ambient+$4^{\circ}C$, B), and high temperature (ambient+$6^{\circ}C$, C). The fresh weight of Chinese cabbage was greater under B and C conditions in the first period and A in the second period, which indicated that GDDs affected the fresh weight considerably. However, leaf growth (number, area, length, and width) did not differ by GDDs. Bolting appeared under A condition in the first period, which was caused by low temperature in the early growth stage. Soft rot was developed under C condition in the second period and all temperature conditions in the third period, which resulted from high temperature in the late stage. Fresh weight increased when GDDs ranged from 587 to 729. However, it decreased when GDDs > 729. The maximum expected yield (16.3 MT/10a) was attained for the growing period of 64 days from transplanting date during which GDDs reached 601. The GDDs for optimum cultivation ranged from 478-724 under which the yield was about 95% (15.5 MT/10a) of maximum fresh weight. Such an optimum condition for GDDs was validated at five main cultivation regions including Jindo, Haenam, Naju, Seosan, and Pyeongtaek in Korea. In these regions, GDDs ranged from 619-719. This suggested that the optimum GDDs for Chinese cabbage cultivation would range from 478-724, which would give the useful information to expect the cultivation periods for ensuring maximum yield.

Prodcutivity of Silage Corn Affected by Planting Dates in the Rice Black-streaked Dwarf Virus Prevalent Area (흑조위축병 발생지역에서 파종기에 따른 Silage 옥수수의 생산성)

  • 이석순;이진모
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.3
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    • pp.249-255
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    • 1987
  • To find out the optimum planting date of silage corn in the rice black-streaked dwarf virus (RBSDV) pre-valent area, a resistant hybrid of Jinjuok and a susceptible hybrid of Suweon 19 were planted at the 8 planting dates from April 1 to June 10 at the la-day intervals. Considering escape of RBSDV and silage yield, the optimum planting date seems to be during the April in both hybrids. At the April plantings silage yield of two hybrids ranged from 17 to 23 tons/ha on the dry matter base due to a lower infection rate and higher tolerance to RBSDV and there was no difference in silage yield between two hybrids. At the May plantings yield of ear, stover, and silage, culm length, and digestible dry matter decreased significantly as planting date delayed due to an increase in RBSDV infection rate in both hybrids although performance of a resistant hybrid of Jinjuok was better than a susceptible hybrid of Suweon 19. At the June 10 planting RBSDV infection rate was low and performance of corn was better than that at the May plantings but poorer than that at the April plantings. The peak of small brown planthopper population occurred in late March or early April, middle June, middle July, and early September although it fluctuated a little yearly. Very low small brown planthopper population during the May may attribute to a lower RBSDV infection and high silage yields at the April plantings because the plants grow enough to be tolerant when infected by RBSDV in middle June. However, at the May plantings plants are too young to be tolerant when infected by RBSDV in middle June.

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Radiation Therapy in Elderly Skin Cancer (노령의 피부암에서 방사선치료)

  • Kim, Jin-Hee
    • Radiation Oncology Journal
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    • v.26 no.2
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    • pp.113-117
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    • 2008
  • Purpose: To evaluate the long term results(local control, survival, failure, and complications) after radiation therapy for skin cancer in elderly patients. Material and Methods: The study spanned from January 1990 to October 2002. Fifteen elderly patients with skin cancer were treated by radiotherapy at the Keimyung University Dongsan Medical Center. The age distribution of the patients surveyed was 72 to 95 years, with a median age of 78.8 years. The pathologic classification of the 15 patients included squamous cell carcinoma(10 patients), basal cell carcinoma(3 patients), verrucous carcinoma(1 patient) and skin adnexal origin carcinoma(1 patient). The most common tumor location was the head(13 patients). The mean tumor diameter was 4.9 cm(range 2 to 9 cm). The radiation dose was delivered via an electron beam of 6 to 15 MeV. The dose range was adjusted to the tumor diameter and depth of tumor invasion. The total radiation dose ranged from $50{\sim}80$ Gy(mean: 66 Gy) with a 2 Gy fractional dose prescribed to the 80% isodose line once a day and 5 times a week. One patient with lymph node metastasis was treated with six MV photon beams boosted with electron beams. The length of the follow-up periods ranged from 10 to 120 months with a median follow-up period of 48 months. Results: The local control rates were 100%(15/15). In addition, the five year disease free survival rate(5YDFS) was 80% and twelve patients(80%) had no recurrence and skin cancer recurrence occurred in 3 patients(20%). Three patients have lived an average of 90 months($68{\sim}120$ months) without recurrence or metastasis. A total of 9 patients who died as a result of other causes had a mean survival time of 55.8 months after radiation therapy. No severe acute or chronic complications were observed after radiation therapy. Only minor complications including radiation dermatitis was treated with supportive care. Conclusion: The results suggest that radiation therapy is an effective and safe treatment method for the treatment of skin cancer in elderly patients who achieved a good survival rate and few minor complications.

The effects of selenium on fetal growth and development in CD-1 mice exposed with mercury for the gestation period (임신 중 수은을 섭취한 CD-1 마우스 태아의 성장발육과 기형발생에 미친 셀레늄의 효과)

  • Kim, Jin-suk;Lee, Sang-mok;Choi, Seok-wha;Lee, Won-chang
    • Korean Journal of Veterinary Research
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    • v.34 no.2
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    • pp.361-368
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    • 1994
  • Teratogenic and embryotoxic effects of mercury have been reported, however, there is little information about possible antidotes against mercury exposure during gestation. In order to evaluate therapeutic effects of selenium as an antidote against mercury poisoning, pregnant CD-1 mice were exposed to methylmercury chloride(20ppm) through the drinking water with treatment of sodium selenite (1.0mg, 2.0mg or 3.0mg/kg b.w., subcutaneously) or BAL(5.0mg/kg b.w., subcutaneously) under the single or combination base as the therapeutic agents from day 6 to 15 of gestation. Fetal growth parameters such as body weight and crown-rump length in the mice exposed to mercury, were reduced as was placental weight compared to those in the control. Treatment of selenium(alone, combination with BAL) reduced the harmful effects induced by mercury on the fetal growth parameters even though no specific relationship between dose and therapeutic effect. The incidence of dead fetuses/resorptions and malformed fetuses(especially cleft palate) was also increased in the mercury only treated group. Selenium treatment demonostrated reduced the incidence of abnormal fetuses under the exposure of mercury. Relative maternal organ weights(liver, kidney, spleen) were increased significantly but relative brain weight was decreased as evidenced by decreased in the mercury treated mice compared to that in the control. A subtle indication of maternal mercury toxicity evidenced by changes of relative maternal organ weights, decreased water and feed consumption were also prevented efficiently by selenium treatment. The present study suggests that methylmercuric chloride is embrytoxic and teratogenic in CD-1 mice when exposured during organogenesis and that selenium administration may have therapeutic application for the treatment of mercury poisoning although more applicable study in human should be performed with caution in the future.

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Germination of Two Rice Cultivars and Several Weed Species (벼와 수종(數種) 논잡초(雜草)의 휴면성(休眠性)과 발아성(發芽性))

  • Kim, Soon-Chul;Moody, Keith
    • Korean Journal of Weed Science
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    • v.9 no.2
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    • pp.116-122
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    • 1989
  • An experiment was carried out at the International Rice Research Institute in 1987 to understand the seed dormancy and germination habit of rice and several weed species. The germinability of the weed seeds just after harvest was variable depending on the species and ranged from 0 to 72%. Two rice cultivars, IR64(lowland type) and UPLRi-5(upland type) had higher than 95% in germination ability throughout the experimental period due to the fact that the rice seeds came from the harvest of the previous season and dormancy had already been overcome. The length of the storage period needed to overcome dormancy at room temperature($25{\pm}2^{\circ}C$) was about 50 days for Echinochloa glabrescens Munro ex Hook, f., more than 60 days for E. crus-galli ssp. hispidula (Retz.) Honda and 20 days for Ludwigia octovalvis(Jacq.) Raven. Seeds of E. colona(L.) Link, Monochoria vaginalis(Burm. f.) Presl, Fimbristylis miliacea(L.) Vahl and Cyperus difformis L. appeared to have no dormancy. Among the nine species M. vaginalis had the lowest germination of less than 1% throughout the experimental period. However, its seed germinated easily when planted in soil. The low germinability of E. glabrescens, E. crus-galli ssp. hispidula and L. octovalvis just after harvest could be overcome through pretreatment of seeds either by soaking in nitric acid(0.1N) for 1 day or removal of the hull in the grass species, the nitric acid treatment being superior. The results imply that germination habit of weed species varied depending on the species through their differential dormancy period or differential germination strategy.

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Japanese Medaka, Oryzias latipes as a Test Animal for Marine Ecotoxicological Evaluation (해양생태독성평가를 위한 표준시험생물로서의 송사리(Oryzias latipes)에 관한 연구)

  • Park Gyung Soo;Yoon Seong Jin;Lee Seung Min;Kim Ae Hyang;Park Soung Yun;Kang Duk Young
    • Korean Journal of Environmental Biology
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    • v.23 no.3 s.59
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    • pp.293-303
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    • 2005
  • Japanese medaka, Oryzias latipes is widely distributed in the North East Asia including Korea, Japan and east China, and commonly used for freshwater toxicity tests and cytotoxicological studies worldwide. In this study, a series of experiments were conducted to identify the potential of the fish as a standard test species for saltwater toxicity evaluation such as marine receiving waters, ocean-dumped materials and sediment pore waters etc. Hatching, growth and mortality rates of the fish were estimated with the wide ranges of salinity from freshwater to seawater (35 psu). Direct exposure of the fertilized eggs in freshwater to the wide ranges of salinity (from 0 to 35 psu) without pre- acclimation to the saltwater revealed no significant differences in hatching rates by salinities (p =0.24). On the other hand, medaka larvae hatched in freshwater and exposed to saltwater directly showed high mortality at > 25 psu treatment groups (p < 0.0001). However, there was no significant difference in mortality of medaka larvae hatched in 13.8 and 14.2 psu at the wide ranges of salinities ($0\~35$ psu). Growth rates of medaka larvae hatched in the above two salinities showed no differences in body length either from 0 to 35 psu treatment groups (p =0.64 for 13.8 psu group and p=0.32 for 14.2 psu group). The number of gill chloride cell in medaka larvae sharply increased when the larvae were exposed to high salinity. Reference tests with zinc chloride revealed 96h $LC_{50}=8.84(7.19\~10.87)mg\;L^{-1}$ using 7~10 day old medaka larvae. These were comparable or better sensitivity in comparison with the other standard test species such as North American sheepshead minnow Cyprinodon variegatus. Based on the results of these experiments, hatching rates and larvalmortality of medaka must be good toxicity parameters for seawater bioassay and the species seems to be a good standard species for both the freshwater and seawater toxicity test.

Effects of Deer Antler on the Regeneration of Peripheral Nerves; About Sprout Formation of Experimentally Transected Sciatic Nerves in Rat (말초신경의 재생에 대한 녹용의 효과; 랫드에서 실험적 절단 좌골신경의 Sprout 형성에 관해)

  • Chang, Byung-Joon;Cho, Ik-Hyun;Choi, Hye-Young;Won, Hui-Young;Park, Chang-Hyun;Bae, Chun-Sik;Choe, Nong-Hoon
    • Applied Microscopy
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    • v.32 no.1
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    • pp.67-80
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    • 2002
  • This study was carried out to investigate the effects of deer antler extract on the regeneration of peripheral nerves. Sprague-Dawley male rats weighing about 300 gm were fed deer antler extract for 1, 2, and 3 weeks per oral (1.5 ml/100 gm B.W.), respectively, once a day and transected both sides of sciatic nerve of each leg. After keeping for 6 hours, sciatic nerves taken from proximal part of transected region were treated with conventional transmission electron microscopical method and then observed with electron microscope. The results obtained were summarized as follows; 1. Sciatic nerves of normal control group were not showing any sprouts and electron dense axolemmal projections were frequently observed. 2. Sciatic nerves of saline treated groups were showing axonal sprouts at the nodes of Ranvier. The length of them was usually short, and numerous vesicles, vacuoles and organelles including neurofilament were contained. The number of nodes of Ranvier containing sprouts from 100 longitudinal sectioned nerve fibers was 29 (29%) in 1 week treated group, 32 (32%) in 2 weeks treated group, and 30 (30%) in 3 weeks treated group, respectively. 3. Sciatic nerves of deer antler treated groups were showing axonal sprouts at the node of Ranvier as well. Although most of the sprouts were short, some sprouts of 2 weeks and 3 weeks treated groups were quite long. Sprouts usually contained numerous vesicles, vacuoles and cell organelles such as neurofilaments and mitochondria. The number of nodes of Ranvier containing sprouts from 100 longitudinal sectioned nerve fibers was 38 (38%) in 1 week treated group, 46 (46%) in 2 weeks treated group, and 48 (48%) in 3 weeks treated group respectively. The results described above explain pretreatment of deer antler extract improves the sprout formation of transected sciatic nerves, and then it suggests deer antler may be effective for the regeneration of peripheral nerves.