• Journal of Environmental Science International
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• v.1 no.1
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• pp.1.1-11
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• 1992

To find heavy metal-specific effects on the photosynthetic apparatus of higher plants, we investigated effects of $CuCl_2$, HgCl_2$ and $ZnCl_2$ on electron transport activity and chlorophyll fluorescence induction kinetics of chloroplasts isolated from barley seedlings. Effects on some related processes such as germination, growth and photosynthetic pigments of the test plants were also studied. Germination and growth rate were inhibited in a concentration-dependent manner by these metals. Mercury was shown to be the most potent inhibitor of germination, growth and biosynthesis of photosynthetic pigments of barley plants. In the inhibition of electron transport activity, quantum yield of PS II, and chlorophyll fluorescence induction kinetics of chloroplasts isolated from barley seedlings, mercury chloride showed more pronounced effects than other two metals. Contrary to the effects of other two metals, mercury chloride increased variable fluorescence significantly and abolished qE in the fluorescence induction kinetics from broken chloroplasts of barley seedlings. This increase in variable fluorescence is due to the inhibition of the electron transport chain after PS ll and the following dark reactions. The inhibition of qE could be attributed to the interruption of pH formation and do-epoxidation of violaxathin to zeaxanthin in thylakoids by mercury. This unique effect of mercury on chlorophyll fluorescence induction pattern could be used as a good indicator for testing the presence and/or the concentration of mercury in the samples contaminated with heavy metals.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.61 no.3
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• pp.222-226
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• 2016

This research was carried out to investigate the germination and seedling growth characteristics of the halophyte Suaeda asparagoides under various salt concentrations, temperatures, and the presence or absence of light S. asparagoides has a non-endospermic dicotyledonous seed structure that is go stone-shaped with a dark brown color and covered by a star-shaped perianth. Germination within the temperature range $5{\sim}40^{\circ}C$ varied from 8% to 65%. The highest germination occurred at $15^{\circ}C$. In seeds exposed to salt concentrations in the range 0~400 mM, the highest germination occurred at 0 mM, and the germination progressively decreased as the salt concentration increased. When the perianth of the seed was removed, the germination increased significantly. In the presence or absence of light, germination under white light was 5~20% higher than that under darkness, which varied according to salt concentrations in the range 0~300 mM. The plant growth of S. asparagoides, evaluated by plant height, branch number, root length, fresh weight, and dry weight, was higher in the concentration range 50~100 mM, and it decreased in the other concentrations. In conclusion, the seeds of S. asparagoides showed higher germination in fresh water at low temperature of approximately $15^{\circ}C$ under light when the perianth was removed, and the plant growth was higher at salt concentrations of 50~100 mM.

• Korean Journal of Medicinal Crop Science
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• v.21 no.5
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• pp.329-333
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• 2013

This study was carried out to compare the characteristics of seed germination and the first stage of growth in Cynanchum wilfordii (Maxim.) by different light conditions such ass fluorescent light, dark and light emitting diode (LED). There was not much difference from the germination rate of C. wilfordii (Maxim.) in different light conditions for 10 days. The highest of germination rate occurred over 91.11 percentage by LED red condition at $26^{\circ}C$. Also mean germination velocity and promptness index were represented the highest results of 2.3 ea/day and 52.67 under red light, respectively. But mean germination time under both LED red and blue light was represented low data that it takes over three days for germinating of seed. Even though handling both LED red and blue lights, the germination uniformity was not constant. These results may help our understanding of variations in germination characteristics for C. wilfordii (Maxim.) seeds treated under different light conditions.

• Journal of the Korean Institute of Landscape Architecture
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• v.15 no.2
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• pp.139-148
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• 1987

Exochorda serratifolia, broad-leaved shrub has beautiful flowers and is resistant to cold, shade and decease. New this native plant is considered to be worth being exploited as the outstanding plant for landscaping and horticulture. So this study was executed to utilize Exochorda serratifolia as the planting material for landscaping and horticulture through the survey of its habitat environment and the experimennt of its seed physiology and germination, vegetative propagation, culture and utilization, etc.. The results are as follows ; 1. The color of the flower is white, blooming in the early and middle of May and the seeds ripen late in Sep.. 2. The elevation, elevation, gradient and direction of the native habitat were 250m, 20-25$^{\circ}$and northern side respectively. 3. The soil pH of the natural habitat was 5.3 and soil fertility was poor. 4. Exochorda serratifolia appeared as indicator within P. densiflora community and its neighboring species were Q. mongolica, Q. dentata, R. mucronulatum, L. obtusiloba, E. oxyphillus, C. heterolphylla, var thunbergii, etc.. 5. The optimum temperature for seed germination was found at 20$^{\circ}C$ and the longer the stratification period at 5$^{\circ}C$ was, the lower the germination rate was. 6. The treat merits of GA and Kinetin increased the seed germination rate, especially under the dark condition but their high concentrations decreased the seed germination rate. 7. The rooted rate of the greenwood cutting was the highest at IBA 100ppm plot of vermiculite bed but its high concentration decreased the rooted rate conspicuously. 8. In the tissue culture, the each 1.0 ppd plot of NAA, Kinetin and NAA 1.0ppm + Kinetin showed the best growth. And the mixture of NAA 1.0ppm and Kinetin showed better growth than the single treatment of NAA or Kinetin did. 9. Transplanted Exochorda serratifolia showed healthy growth with shaded environmental condition(42.1% light intensity), therefore they can be cultivated as a shade tolerant landscape plant. 10. It was considered that Exochorda serratifolia was applicable to group planting at shade places or under trees in parks, homes, etc..

• Korean Journal of Medicinal Crop Science
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• v.4 no.2
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• pp.163-171
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• 1996

The influences of temperature, harvest time, sowing time, seeding media, moisture content of media and light on the seed germination of Lycoris are as follows, Both L. koreana and L. aurea had over 90% seed setting, and seed maturity came in September or early October. It took six months to have over 60% seed germination. The optimum germination temperature was $25^{\circ}C$, and the alternating treatment was effective at $20{\rightleftarrows}25^{\circ}C$. The optimum harvest-time was around Sep. 20, and the optimum seed-time was immediately after havest. The optimum seeding media was sand loam, and the optimum moisture content was PF 1. 9/400ml seeding media. The dark condition was more effective for the seed germination than the light condition.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.6
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• pp.507-512
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• 1993

Pre-sowing treatments of tobacco seeds (cv. NC82) were evaluated as a means of improving the speed of emergence, early seedling growth and uniformity of the seedlings. Seeds were treated through the use of polyethylene glycol 6000(PEG), solid matrix priming(SMP), chilling and dark-preincubati(DPI). Under the laboratory condition of 15$^{\circ}C$, all the treated seeds germinated more rapid and the time to 50% germination(T50) reduced than control. Among the treatments, SMP was the best to reduce the T50 for germination. All the treatments did not affect the final germination percentage, while the chilling showed of little benefit for germination and T50. Under the greenhouse condition, seedling from SMP emerged earlier and more uniformity and also greater mean fresh and dry weight showed than other treatments. The advantage of T50 from SMP was affected more in temperature than in water content of Agro-Lig during the treatment.

• Horticultural Science & Technology
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• v.32 no.4
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• pp.550-557
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• 2014

Heloniopsis koreana and Heloniopsis tubiflora (Melanthiaceae) are endemic herbaceous species of the Korean Peninsula. The Melanthiaceae family has been described as having seeds with small, underdeveloped embryos at the time of dispersal, and morphological (MD) or morphophysiological dormancy (MPD). However, there are few reports on embryo growth, morphology, and seed germination in Heloniopsis species. The aims of this study were to investigate embryo growth and seed dormancy, and to determine the type of dormancy exhibited by these species. The effects of incubation temperatures, light conditions, and gibberellic acid ($GA_3$) on dormancy break and seed germination were tested. Freshly matured seeds of the two species had small embryos that occupied about 9-11% of the length of the endosperm, and which increased by more than 300% in length before radicle emergence, indicating that the embryos are underdeveloped at the time of dispersal. Embryos in the seeds grew under warm temperature regimes (between $25/15^{\circ}C$ and $30/20^{\circ}C$). $GA_3$ application (tested only in the light) overcame seed dormancy and promoted germination. Approximately 30% of the seeds of H. koreana and approximately 40% of the seeds of H. tubiflora germinated in suitable environmental conditions (light and temperature) within 4 weeks. Therefore, 30-40% of the seeds of the two species exhibited MD, and the rest of the seeds had non-deep simple MPD. Light was found to be one of the critical factors for germination because no seed of either of the two Heloniopsis species germinated under constant dark conditions, and thus, these species have the potential to form a persistent soil seed bank. Understanding these germination requirements will help in development of effective strategies to increase the establishment of seedlings in their native habitat.

• Korean Journal Plant Pathology
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• v.13 no.4
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• pp.210-214
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• 1997

The experiment was conducted to obtain basic informations on the effects of key environments and fungicides on the sporulation, conidial germination and hitial growth of the hyphae of grape powdery mildew fungus Uncinula necator. Maximum sporulation occurred at RH 75~96% in vitro adiusted with sulfuric acid solution. Conidial germination and initial growth of the hyphae were better at 5% water agar than distilled water, and best at $26^{\circ}C$ and pH 5.0 of the substrates. Germination occurred equally well at light intensity of below 100 lux or dark condition, which was better at water agar supplemented with leaf extract 30% than pure water agar. The water agar supplemented with triazole fungicides reduced conidial germinations and initial growth of the hyphae signifcantly, in proportion to the increase in concentration of the fungicides, but the maguitude of reduction depended on the fungicides. Particularly in myclobutanil, reduction rate was very low as increased in concentration.

• Korean Journal of Plant Resources
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• v.21 no.4
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• pp.341-345
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• 2008

Two orchid species of Calanthe discolor Lindl. and C. discolor for. sieboldii (Decne.) Ohwi, which have different form flower color and size. They were crossed in mid April by artificial pollination, and the F1 hybrid seeds were collected mid October. Germination of seeds was investigated on pre-treatment of seeds and under the various environmental conditions. Germination was promoted by moisture absorption and ultrasonic treatment of seeds. Dark culture of F1 hybrid seeds enhanced germination and protocorm formation, and development into seedlings compared with light culture. Although, plant growth regulators such as NAA and BA had a slightly promotive effect on seed germination and protocorm growth, regenerated seeding were showed abnormal growth patterns. Regenerated F1 hybrid plantlets were successfully transferred to pot.

• Korean Journal of Food Science and Technology
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• v.17 no.5
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• pp.377-388
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• 1985

The changes in protein fractions and its gel electrophoretic pattern of rapeseeds (Brassica napus L) were investigated during germination at $25^{\circ}C$ under dark condition. The major protein fraction was found to be albumin 25.0%) and globulin (24.6%). Both fractions were decreased throughout germination, particularly significant for albumin, while prolamine(2.2%) and glutelin (1.8%) showed an initial decrease followed by a slow increase at the later stage of germination. The initial 5-6 peaks of gel electrophoresis were reduced to a few after 45 hours. The absorption spectrum at the range of 400-700 nm showed a significant increase in absorbance for sodium hexametaphosphate (SHMP) extract of rapeseeds. The protein extractability with SHMP was not significantly affected by germination.