• 대한미생물학회지
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• 제6권1호
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• pp.41-60
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• 1971

The author prepared a tuberculin sample using synthetic medium for the test material in this experiment. For the test animals, guinea pigs, healthy calf sensitized with heat killed tubercle bacilli, naturally infected dairy cattle, goat, and calf were used. A reduced reactivity to tuberculin was observed when the second tuberculin test was done within 28 days after the first. The results obtained are summarized as follows. 1. The experimental HCSM tuberculin was shown the similar activities as the control products of standard tuberculin. 2. The potency of the experimental tuberculin preserved at $5^{\circ}C$ in refrigerator maintained the activity for three years. 3. By the intradermal test, the experimental tuberculin was proved to be specific to detect tuberculous dairy cattles and goats. 4. The appearance of skin reaction to tuberculin in naturally infected dairy cattles, goat and beef cattles was soft and diffuse swellings or circumscribed swellings accompanied by induration. 5. In the case of observing tuberculous dairy cattles, goats and beef cattles, as the criterion of the reaction to the caudal fold test, results were taken as positive when findings are either hard and circumscribed or soft and diffuse swellings of 3 mm at least in size. 6. The acid fast organisms isolated from tuberculin positive animals were identified mostly as M. tuberculosis var. bovis. 7. When the tuberculin test was repeated in the tuberculin positive animals, the reactivity decreased rapidly, lasting 4 weeks at least.

• 한국임상수의학회지
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• 제24권2호
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• pp.177-181
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• 2007

In the present study, a total of 547 half milk samples were collected from 274 dairy goats to perform somatic cell counts (SCC) and California mastitis test (CMT). Milk smear was stained with Pyronin Y-Methyl Green stain were classified into either epithelial or blood cells, etc. Of the 547 halves the percentage of CMT negative milk samples were 86%. Among these, 58.2% were CMT negative with SCC<500,000/ml, while 27.8% were CMT negative with SCC>500,000 ml. As expected, CMT score increased with the increase of SCC. The number of epithelial cells decreased with the increasing number of somatic cells, while the opposite was observed with the number of blood cells. These results indicate that the critical point in milk quality & CMT should be considered on the false (pseudo-SC) SCC in dairy goat.

• 한국동물위생학회지
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• 제44권1호
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• pp.11-14
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• 2021

Seroprevalence of Coxiella burnetii in cattle and goats from southern region of Korea was analyzed. From January to December 2020, 1,409 samples of bulk-tank milk, cattle serum and goat serum were collected and analyzed using ELISA. The prevalence of antibodies in collected was 72.6% (501/682), 4.1% (21/515) and 10.4% (22/212). By age, the seroprevalence of cattle was 2.4%, 4.1%, 5.3% (<2 years, 2~5 years, ≥6 years). In bulk-tank milk of dairy cattle according to region was Gyeongnam 45.5%, Gyeongbuk 77.5%, Daegu 70.0%, Jeonnam 50.0% and Chungbuk 50.0%. And seroprevalence of goats was 10.4% (22/212).

• Asian-Australasian Journal of Animal Sciences
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• 제26권3호
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• pp.309-315
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• 2013

MicroRNAs are a class of endogenous small RNAs that play important roles in post-transcriptional gene regulation by directing degradation of mRNAs or facilitating repression of target gene translation. In this study, three small RNA cDNA libraries from the mammary gland tissues of Laoshan dairy goats (Capra hircus) were constructed and sequenced, individually. Through Solexa high-throughput sequencing and bioinformatics analysis, we obtained 50 presumptive novel miRNAs candidates, and 55,448 putative target genes were predicted. GO annotations and KEGG pathway analyses showed the majority of target genes were involved in various biological processes and metabolic pathways. Our results discovered more information about the regulation network between miRNAs and mRNAs and paved a foundation for the molecular genetics of mammary gland development in goats.

• Asian-Australasian Journal of Animal Sciences
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• 제32권8_spc호
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• pp.1275-1283
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• 2019

Goats (Capra hircus) were domesticated during the late Neolithic, approximately 10,500 years ago, and humans exerted minor selection pressure until fairly recently. Probably the largest genetic change occurring over the millennia happened via natural selection and random genetic drift, the latter causing genes to be fixed in small and isolated populations. Recent human-influenced genetic changes have occurred through biometrics and genomics. For the most part, biometrics has concentrated upon the refining of estimates of heritabilities and genetic correlations. Heritabilities are instrumental in the calculation of estimated breeding values and genetic correlations are necessary in the construction of selection indices that account for changes in multiple traits under selection at one time. Early genomic studies focused upon microsatellite markers, which are short tandem repeats of nucleic acids and which are detected using polymerase chain reaction primers flanking the microsatellite. Microsatellite markers have been very important in parentage verification, which can impact genetic progress. Additionally, microsatellite markers have been a useful tool in assessing genetic diversity between and among breeds, which is important in the conservation of minor breeds. Single nucleotide polymorphisms are a new genomic tool that have refined classical BLUP methodology (biometric) to provide more accurate genomic estimated breeding values, provided a large reference population is available.

• 한국초지조사료학회지
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• 제29권4호
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• pp.383-388
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• 2009

본 연구는 혼파초지에서 유 산양을 방목 이용하였을 때의 건물섭취량, 증체량 및 두당 방목초지의 소요면적을 산출하고자 2007년 4월부터 2008년 6월까지 충남대학교 동물사육장내 혼파초지에서 수행하였다. 공시초지는 2006년 9월 21일 orchardgrass (40%) + tall fescue (20%) + perennial ryegrass (10%) + alfalfa (15%) + red clover (15%)를 ha당 30 kg 파종하여 경운초지로 조성한 혼파초지에서 수행하였다. 방목면적은 총 $5,000\;m^2$로 4목구(구당 평균 $1,250\;m^2$)의 윤환 방목으로 12회 방목하였다. 공시축은 Saanen종으로 13두(평균 31.1 kg)를 방목하였다. 방목기간 중 월 별로 조사한 혼파초지의 평균 조단백질 함량은 20.4%, NDF와 ADF 함량은 각각 65.3%와 31.1%이었으며, 건물소화율은 68.9%이었다. 두당 평균 건물섭취량은 1.253 kg 이었으며, 체중 당 3.01%을 섭취하였다. 방목기간 (184일)의 두당 평균 증체량은 17.4 kg이었고, 일당증체량은 98g이었다. 유 산양(체중 50 kg 기준)의 두당 방목초지의 소요면적은 $467.7\;m^2$로 산출되었는데 이를 Animal unit (AU/ha)로 환산하면 약 2.14 AU/ha에 해당하였다.

• 한국초지조사료학회지
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• 제30권1호
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• pp.43-48
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• 2010

본 연구는 2008년 4월부터 12월까지 충남대학교 생명과학대학 내 부속 환경조절축사에서 수행하였다. 공시된 건초는 수입 Timothy 건초(수입건초), 혼합목건초 (목건초) 및 혼합 야건초(야건초)의 3처리이며 공시가축은 유 산양 (Saanen종, 평균체중 24.2 kg, male) 12두로 화학적 성분, 건물소화율, 건물섭취량과 질소 및 에너지이용성을 비교 분석하였다. 조단백질 함량은 목건초가 야건초나 수입건초에 비하여 높은 결과를 나타내었으나(p<0.05), NDF, ADF, cellulose 및 lignin 함량은 반대로 수입건초와 야건초가 높았다 (p<0.05). 건물 섭취량은 목건초 (30.7 g/BWkg)가 야건초 (24.6 g/BWkg)와 수입건초 (24.4g/BWkg)에 높은 결과를 보였으나 (p<0.05), 야건초와 수입건초 간에 유의적인 차이는 없었다. 건물소화율은 목건초가 69.8%로 야건초(62.2%)와 수입건초 (60.8%)에 높은 결과를 보였다 (p<0.05). 그러나 야건초와 수입건초간에 건물소화율의 유의적인 차이는 없었다. 외관상 생물가는 목건초가 56.0%으로 야건초 (49.2%), 수입건초 (47.7%) 순으로 나타났다 (p<0.05). 외관상 대사에너지 축적률은 목건초가 63.4%로 야건초 (54.4%)나 수입건초 (53.6%)에 비하여 높은 결과를 보였으나 (p<0.05), 역시 야건초와 수입건초 간에 유의적인 차이는 없었다. 이상의 결과로 보아, 유 산양에 의한 건물섭취량, 건물소화율과 질소 및 에너지의 이용성은 목건초가 가장 높았으며, 야건초는 수입건초에 비하여 건물소화율과 외관상 생물가가 높은 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권9호
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• pp.1305-1313
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• 2006

Five male dairy goats (Saanen), 4.6 month old with a body weight of 21.4 ($SD{\pm}1.6$) kg, were used to examine 5 dietary urea treatments in a $5{\times}5$ Latin Square experimental design. The five levels of urea were 10, 20, 30, 40 and 50 g urea/kg DM of steam-treated oil palm fronds (OPF) and dry matter intake tended (p>0.05) to increase with increasing urea supplementation up to 30 g/kg OPF (77.7 g/kg $BW^{0.75}$), but decreased (p<0.05) with 40 and 50 g urea/kg OPF (67.4 and 63.7 g/kg BW0.75, respectively) supplementation. Similarly, dry matter, organic matter, crude protein, neutral detergent fiber and hemicellulose digestibilities increased (p<0.05) with the addition of urea to 30 g/kg OPF but thereafter decreased (p<0.05) with 40 and 50 g/kg OPF. Ruminal pH, ruminal $NH_3$-N concentration and plasma urea concentration increased linearly (p<0.01) and quadratically (p<0.01) as a consequence of addition of urea to the diet. Excretion of total purine derivatives (PD) by goats fed 30 g of urea/kg OPF was highest (p<0.05) followed by goats fed 20, 40, 10 and 50 g of urea/kg OPF. Microbial N (g N/day) and efficiency of microbial N supply expressed as g N/kg organic matter apparently digested in the rumen were higher (p<0.05) in goats fed 30 g of urea/kg OPF (5.5 g N/day and 22.0 g N/kg DOMR, respectively) than in goats on 10 and 50 g of urea/kg OPF treatments. However, the former did not differ from goats fed 20 g of urea/kg OPF (3.9 g N/day and 16.6 g N/kg DMOR, respectively). Ruminal VFA concentration, protein/energy ratio, N absorption and N retention increased (p<0.05) with the addition of urea to the diet up to 30 g/kg OPF but decreased (p<0.05) with 40 and 50 g/kg OPF. This implies that the optimal level of urea supplementation in an OPF based diet was about 30 g urea/kg OPF.

• Asian-Australasian Journal of Animal Sciences
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• 제31권2호
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• pp.225-229
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• 2018

Objective: This experiment was designed to evaluate the effect of supplementation with soybean or canola oil on milk production and the composition of long chain fatty acids as well as weight changes in the goats and their kids. Methods: Thirty nine mulitparous crossed Alpine${\times}$Nubian goats (initial body weight [BW] $43.5{\pm}1.7kg$) from the day of parturition were assigned to the treatments: grazing control (n = 15); grazing plus 20 mL/goat/d of supplemental soybean oil (n = 12); and grazing plus 20 mL/goat/d of supplemental canola oil (n = 12) from November 26, 2014 to March 7, 2015. The planned contrasts were: CI (control vs supplemented with oils); CII (soybean vs canola oil) to compare the treatment effects. Results: The vegetable oil supplementation reduced weight losses in lactating goats (CI: -0.060 vs 0.090 kg/d; p = 0.03) but did not improve milk production or affect kids' growth. The content of C4, C6, C8, C10, C11, C14, and C18:1n9t in the milk was increased (p<0.05) with respect to control. However, C12, C14, C16, C18, C18:1n9c, C18:2n6c, and C18:3n3 were reduced (p<0.05) in supplemented goats. Conjugated linoleic acid (CLA) was increased (p<0.05) in goats supplemented with oils compared to the control group. Conclusion: Supplementation with 20 mL/d of soybean or canola oil did not affect milk production or kids' performance; however, it increased CLA concentration and reduced the reduced weight losses in lactating goats.

• 대한수의학회지
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• 제44권1호
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• pp.151-157
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• 2004

Samples of milk were collected from 425 halves of 216 dairy goats in Chonnam province over a period of January through August 2003. Bacterial isolation was carried out on those samples, and their antimicrobial susceptibility was tested. Bacteria were isolated from 166 milk samples (39.1%), either singly (74.7%) or in combination (25.3%). Of the 220 isolates, Staphylococcus spp. was the most prevalent (82.6%), followed by Streptococcus spp. (2.7%), Corynebacterium spp. (1.8%), Enterococcus spp. (1.8%), and Pasteurella spp. (1.8%). Of the 11 species identified from the 182 isolates of Staphylococcus spp., the most frequent species identified were S. epidermidis (28.6%) followed by S. chromogens (14.8%), S. haemolyticus (12.6%), S. aureus (12.1%), S. capitis (8.2%), S. lentus (8.2%), S. hyicus (4.4%), S. simulans (4.4%), S. caprae (2.8%), S. hominis (2.8%) and S. warneri (1.1%). Antimicrobial sensitivity test revealed that most isolates were highly susceptible to 11 antimicrobial agents (96.4 ~ 80.9%), while most isolates were resistant to penicillin.