• Journal of Korean Society of Water and Wastewater
• /
• v.20 no.2
• /
• pp.245-255
• /
• 2006

Dissolved air flotation is a solid-liquid separation system that uses fine bubbles rising from bottom to remove particles in water. In order to enhance the flotation velocity and removal efficiency of flocs in the flotation process, we tried to obtain pretreatment conditions for the optimum DAF process operation by comparing and evaluating features of actual floc formation and flotation velocity etc, according to coagulant types and conditions for flocculation mixing intensity by using PIA, PDA, and FSA. Accordingly, generating big flocs that have low density at low flocculation mixing intensity may reduce treatment efficiency. In addition, generating small flocs at high flocculation mixing intensity makes floc-bubbles smaller, which reduces flotation velocity, In this study, it was found that high flocculation mixing intensity could not remove the remaining micro-particles after flocculation, which had negative effects on treated water quality, Therefore, in order to enhance treatment efficiency in a flotation process, flocculation mixing intensity around $50sec^{-1}$ is effective.

• Journal of Korean Society of Water and Wastewater
• /
• v.20 no.2
• /
• pp.303-309
• /
• 2006

Yongdam reservoir located in Jeoliabuk-do has had a lot of concerns for its algal blooming since it has started to fill water. Many water utilities near Yongdam area use reservoirs even though they have some problems with certain algae that cause tastes and odors and clog filters. In this research, dissolved air flotation (DAF) technology was examined for feasibility for removal of algae. OAF can save the capital cost for its compactness, because its hydraulic loadings (overflow rates) are 10 times higher than sedimentation, and hydraulic detention times are much shorter, typically 5 to 15 minutes. As a result of this research, PAC is effective rather than Alum to DAF for pretreatment. Higher DOC plays an important role to change zetapotential negatively to inhibit destabilization of particle to coagulation. The length of pipeline to carry pressurized water into reactor does not affect reaction.

• Journal of Korean Society of Water and Wastewater
• /
• v.32 no.6
• /
• pp.479-485
• /
• 2018

Large amounts of oily wastewater discharged from various industrial operations (petroleum refining, machinery industries and chemical industries) cause serious pollution in the aquatic environment. Although dissolved air flotation (DAF) separating oil pollutants using microbubbles represents current practice, bubble size cannot be selectively controlled, and lots of power is required to generate microbubbles. Therefore, to investigate performance of the DAF process, this study examined the distribution of different sizes of microbubbles resulting from changes in physical shear force via modifying shapes of a slit-nozzle without an additional power supply. Three types of slit-nozzles (different angle, shape and length of the slit-nozzle) were used to analyze the distribution of bubble size. At a slit angle of $60^{\circ}$, shear force was 4.29 times higher than a conventional slit, and particle size distribution (PSD) in the range between 2 and $20{\mu}m$ more than doubled. Treatment efficiency of synthetic oily wastewater through the coagulation-DAF process achieved 90% removal of COD by injecting $FeCl_3$ and PACl of 250 mg/L and 100 mg/L, respectively, and the same performance resulted using $FeCl_3$ of 200 mg/L and PACl of 80 mg/L employing a slit-nozzle angle of $60^{\circ}$. This study shows that a coagulation-DAF process using a modified slit-nozzle can improve the pre-treatment of oily wastewater.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.20
• /
• pp.100-106
• /
• 1975

Germinating ability of green seed in maturing period was investigated to develop the technics on generation shortening. Rapeseed did not germinate until 40 days after flowering (=DAF) in green seed and 50 DAF in dried seed. Effective germination to be utilized for generation shortening came on 55 DAF for dried seed by heating and on 75 DAF for green seed. The periodic time of seed dormancy in maturing period had two cycles and rest period was considered that it was different according to the ecological types of early, middle and late maturity.

• Reproductive and Developmental Biology
• /
• v.39 no.3
• /
• pp.59-67
• /
• 2015

Galactose-${\alpha}1,3$-galactose (${\alpha}1,3$-Gal) epitope is synthesized at a high concentration on the surface of pig cells by ${\alpha}1,3$-galactosyltransferase gene (GGTA1). The ${\alpha}1,3$-Gal is responsible for hyperacute rejection in pig-to-human xenotransplantation. The generation of transgenic pigs as organ donors for humans is necessary to eliminate the GGTA1 gene that synthesize $Gal{\alpha}$(1,3)Gal. To prevent hyperacute graft rejection in pig-to-human xenotransplantation, previously, we developed ${\alpha}1,3$-galactosyltransferase gene-knock-out somatic cell by homologous recombination. In this study, we established cell lines of ${\alpha}1,3$-GT knock-out expressing hDAF and hHT gene from minipig fibroblasts to apply somatic cell nuclear transfer. The hDAF and hHT mRNA were expressed in the knock-in somatic cells and ${\alpha}1,3$-GT mRNA was suppressed. However, the knock-in somatic cells were increased resistance to human serum-mediated cytolysis.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.53 no.1
• /
• pp.34-41
• /
• 2008

This study was conducted to investigate antioxidant component content during maturity for judgement of optimum harvest time in black soybean. For high-functional black soybean production, accumulation pattern of isoflavone and saponin contents and anti oxidative activity according to maturity stage were investigated. Varieties used in this experiment were Ilpumgemojeongkong and heukcheongkong, which are the recommended black soybean in Korea. Isoflavone and saponin contents during maturity period in black soybean was the highest at $6{\sim}7$ days earlier than general harvesting time. It was indicated that optimum harvesting time for high quality soybean were $3{\sim}7$ days earlier than harvesting time for higher yield. As a result of investigation about accumulation pattern of antioxidant components by maturity stages in seed, total isoflavone content was the highest at 61 DAF in Ilpumgeomjeongkong and at 77 DAF in Heukcheongkong. Contents of total saponin were the highest at 61 DAF and at 71 DAF, respectively. In case of leaf, total isoflavone content was the highest at 55 DAF in Ilpumgeomjeongkong and Heukcheongkong. Contents of total saponin were the highest at 18 DAF and at $55{\sim}71$ DAF, respectively. It showed that black soybean's leaf could be developed as a new health food material, owing to high contents of antioxidant components and biological activity and it's suitable harvest time was at $R_7$.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.12
• /
• pp.261-267
• /
• 2020

The demand for faster, lighter, and thinner portable electronic devices has brought about a change in semiconductor packaging technology. In response, a stacked chip-scale package(SCSP) is used widely in the assembly industry. One of the key materials for SCSP is a die-attach film (DAF). Excellent flowability is needed for DAF for successful die attachment without voids. For DAF with high flowability, two-step curing is often required to reduce a cure crack, but one-step curing is needed to reduce the processing time. In this study, DAF composition was categorized into three groups: cure (epoxy resins), soft (rubbers), hard (phenoxy resin, silica) component. The effect of the composition on a cure crack was examined when one-step curing was applied. The die-attach void and flowability were also assessed. The cure crack decreased as the amount of hard components decreased. Die-attach voids also decreased as the amount of hard components decreased. Moreover, the decrease in cure component became important when the amount of hard component was small. The flowability was evaluated using high-temperature storage modulus and bleed-out. A decrease in the amount of hard components was critical for the low storage modulus at 100℃. An increase in cure component and a decrease in hard component were important for the high bleed-out at 120℃(BL-120).

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
• /
• 1999.11b
• /
• pp.164-171
• /
• 1999

A new technique for recycling process water was developed in order to reduce the calcium hardness of the closed OCC recycling system. Calcium ions present in the white water were precipitated as calcium carbonate by a reaction with sodium carbonate and the CaCO$_3$precipitates were easily removed from the system by a dissolved air flotation(DAF) method. After the DAF stage, CO$_2$-gas was purged into the water because the pH of Na$_2$CO$_3$-treated white water was reduced to neutral by CO$_2$gas. Since CaCO$_3$precipitate tends to stick onto the fine fiber surface and then is selectively removed from the water, a proper amount of suspended solid in the process water acts as an important factor in deciding the removal efficiency. By the application of Na$_2$CO$_3$addition - DAF - CO$_2$purging to the short circulated white water the calcium hardness was significantly reduced by 92% and more. The removal of calcium ions with fine fibers led to drainage improvement, reduction of fresh water consumption, and enhanced efficiency of wet-end chemicals.

• Natural Product Sciences
• /
• v.22 no.3
• /
• pp.201-208
• /
• 2016

Here in this study, we investigated the lifespan-extending effect and underlying mechanism of methanolic extract of Moringa olelifa leaves (MML) using Caenorhabditis elegans (C. elegans) model system. To define the longevity properties of MML we conducted lifespan assay and MML showed significant increase in lifespan under normal culture condition. In addition, MML elevated stress tolerance of C. elegans to endure against thermal, oxidative and osmotic stress conditions. Our data also revealed that increased activities of antioxidant enzymes and expressions of stress resistance proteins were attributed to MML-mediated enhanced stress resistance. We further investigated the involvement of MML on the aging-related factors such as growth, food intake, fertility, and motility. Interestingly, MML significantly reduced growth and egg-laying, suggesting these factors were closely linked with MML-mediated longevity. We also observed the movement of aged worms to estimate the effects of MML on the health span. Herein, MML efficiently elevated motility of aged worms, indicating MML may affect health span as well as lifespan. Our genetic analysis using knockout mutants showed that lifespan-extension activity of MML was interconnected with several genes such as skn-1, sir-2.1, daf-2, age-1 and daf-16. Based on these results, we could conclude that MML prolongs the lifespan of worms via activation of SKN-1 and SIR-2.1 and inhibition of insulin/IGF pathway, followed by DAF-16 activation.

• Journal of Applied Biological Chemistry
• /
• v.55 no.4
• /
• pp.227-234
• /
• 2012

We observed that oil began to accumulate at 25 seed days after flowering (DAF) and reached the maximum potential at 35 seed DAF of oilseed rape, and the greatest weight of 100 seeds was obtained at 35 seed DAF. To survey a broad analysis of gene expression in developing embryos of Brassica napus, the Bn 300k microarray have been constructed. The Bn 300k Microarrary was designed from 80,696 unigenes clustered from 543,448 ESTs and 780 cDNA at NCBI. These arrays have been hybridized in a series of experiments with probes derived from seeds and leaf of B. napus. Approximately 8.5% of the 7,000 genes were expressed as ratios 2-fold higher in seed (25 DAF) than leaves and 0.4% at ratios 10. Also we observed that storage and cell differentiation-related genes were highly expressed at 10 DAF, whereas energy-related genes including fatty acid metabolism were increased up depending on seed maturation using Microarray, which was confirmed by reverse transcriptase polymerase chain reaction. These results suggest that B. napus arrays provide a very useful data set of seed-specific expression that can be further analyzed by examination of the promoter regions of these genes and help our understanding of the complex regulatory network in developing seeds.