• Title/Summary/Keyword: cystine

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Exceptionally stable green-synthesized gold nanoparticles for highly sensitive and selective colorimetric detection of trace metal ions and volatile aromatic compounds

  • Singh, Karanveer;Kukkar, Deepak;Singh, Ravinder;Kukkar, Preeti;Kim, Ki-Hyun
    • Journal of Industrial and Engineering Chemistry
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    • v.68
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    • pp.33-41
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    • 2018
  • The manuscript reports synthesis of exceptionally stable gold nanoparticles (GNPs) using Momordica charantia fruit extract. The synthesis approach was optimized by refining three experimental variables including source of the fruit extract (peel, seed, and seed coat), pH of the solution, and temperature of the reaction medium. As synthesized GNPs showed excellent stability against various thiolated compounds (e.g., thioglycolic acid, thiourea, ${\text\tiny{L}}-cystine$, 1-dodecanethiol, and cysteamine hydrochloride). Moreover, these nanoparticles showed distinctive colorimetric responses against $Cd^{2+}$ and thiophenol (TP) from their potential interferences. The limit of detection (LOD) values for $Cd^{2+}$ and TP were determined as 0.186 and $0.154{\mu}M$, respectively.

The Compositions of Fatty Acid and Amino Acid and Storage Property in Dried Persimmons (곶감의 지방산 및 아미노산 조성과 저장성)

  • Mun, Gwang-Deok;Kim, Jong-Guk;Kim, Jun-Han
    • Food Science and Preservation
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    • v.4 no.1
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    • pp.1-10
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    • 1997
  • This study was performed to determine chemical compositions of dried persimmons stored with plastic film at low temperature and the effect of film packaging on dried persimmons during storage. The moisture contents of dried persimmons were 30 to 36% and alcohol contents were 264 to 318 mg/100g and acetaldehyde contents were 25 to 40mg/100g. Total lipid contents of dried persimmon were 626 to 869mg/100g and oleic, palmitic, linolenic, and palmitoleic acid were major fatty acids in total lipids. Major amino acids were glutamic acid, arginine, cystine and aspartic acid. From the result of storage experiment, non-packed group was that hardness was high as decreased in moisture content. In case of film packed groups stored at 5$^{\circ}C$, self life was longer than non-packed and packed groups stored at room temperature. But, it need to develop available packing material and storage method.

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Metabolite Profiling of Serum from Patients with Tuberculosis

  • Park, Hee-Bin;Yoo, Min-Gyu;Choi, Sangho;Kim, Seong-Han;Chu, Hyuk
    • Microbiology and Biotechnology Letters
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    • v.49 no.2
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    • pp.264-268
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    • 2021
  • Tuberculosis (TB) is a major infectious disease that threatens the life and health of people globally. Here, we performed a metabolomic analysis of serum samples from patients with intractable TB to identify biomarkers that might shorten the TB treatment period. Serum samples collected at the commencement of patients' treatment and healthy controls were analyzed using the capillary electrophoresis and time-of-flight mass spectrometry metabolome analysis method. The analysis identified the metabolites cystine, kynurenine, glyceric acid, and cystathionine, which might be useful markers for monitoring the TB treatment course. Furthermore, our research may provide experimental data to develop potential biomarkers in the TB treatment course.

Effect of Nitrogen Sources on Mycelial Growth and Sclerotial Formation of Rhizoctonia solani Causing Rice Sheath Blight (벼 잎집무늬마름병균의 균사생장 및 균핵형성에 미치는 질소원의 효과)

  • Park Kyung Seok;Chung Bong Koo
    • Korean Journal Plant Pathology
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    • v.1 no.1
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    • pp.44-50
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    • 1985
  • Effect of nitrogen sources on mycelial growth and sclerotial formation of Rhizoctonia solani causing rice sheath blight was studied by using sixty isolates from diseased samples of different localities in Chungbuk area. Based on the cultural characters, pathogenicity and sclerotial formation, three isolates of the fungus were selected. The virulence of the three isolates varied based on the percent of seeds germination, seedling blights and length and lesion number of lesions. As nitrogen sources, arginine, alanine, urea, ammonium sulfate and sodium nitrate were more effective for mycelial growth and sclerotial formation, than were cystine, methionine, lysine, histidine, tryptophan and thyrosine. The mycelium of R. so/ani grew well in a lower concentration of urea, sodium nitrate, arginine, ammonium sulfate and lysine, but in a higher concentration of proline, histidine, and alanine. In all nitrogen sources tested, high concentration of nitrogen nutrients greatly stimulated sclerotial formation of the fungus. Arginine, urea, and ammonium sulfate increased virulence of the fungus. All the isolates grown on the basic medium plus proline produced large sclerotia. Mycelial growth of the fungus was not well in the tryptophan-added medium, showing brown discoloration of medium.

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Studies on the Changes in Chlorophyll, Free Amino Acid and Vitamin C Content of Soybean Sprouts during Circulation Periods (콩나물의 유통과정중(流通過程中) Chlorophyll, Amino산(酸) 및 Vitamin C의 변화(變化)에 관(關)한 연구(硏究))

  • Kim, Soon-Dong;Jang, Bong-Hae;Kim, Hyae-Sook;Ha, Kyu-Hyen;Kang, Kyung-Soon;Kim, Duck-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.11 no.3
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    • pp.57-62
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    • 1982
  • This study was conducted to investigate the changes in plant state, chlorophyll, free amino acids and vitamin C content of soybean sprouts during circulation periods. The experiment was carried out under the indoor natural light and complete darkness at $20^{\circ}C$. The results are summarized as follows; Soybean sprout was grown about one cm in the darkness for 7 or 24 hours, but under the indoor natural light it was not grown. Weight was decreased both in the darkness and under the light. In the darkness the color of sprouts were not changed, but under the light the color was changed in 7 or 24 hours. Chlorophyll contents was increased rapidly by light in the cotyledon. Chlorophyll formation brought about loss not only in amino acids but also porphyrins, but it did not in the amino acids of hypocotyl. The kinds of amino acids being brought about loss under the light were cystine, aspartic acid, serine, histidine and glycine. The 22$\sim$27% of vitamin C was lost in cotyledon by 7 hours exposured light and in hypocotyl after 5 hours.

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Changes on the Components of Salted Eggplants during Storage (저장방법에 따른 절임 가지(시키부품종)의 성분 변화)

  • Nam Hak-Sik;Kim Nam-Woo;Um Young-Chul;Shin Seung-Ryeul
    • Food Science and Preservation
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    • v.11 no.3
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    • pp.293-298
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    • 2004
  • This study was carried out to investigate the changes on the components of salted eggplant(shikibu) during storage. The contents of total and reducing sugars were decreased during storage, and the tendency of decrease was lower in the salted eggplants of vacuum packing storage than in the salted eggplants of rice bran immersion storage. The contents of acetic and malic acid were higher than other organic acids. The content of acetic acid was increased during storage, but malic acid was decrease. The major amino acid were valine, leucine, aspartic acid, glutamic acid. The content of essential amino acids was increased during storage. Aspartic acid, alanine, cystine and proline were high content of free amino acids. The contents of phosphoserine, taurine, Y-aminoisobutyric acid and hydroxyproline were higher than others. Posphoserine content was decreased during storage, but Y-aminoisobutyric acid was increased to 5 times of fresh eggplant at 20 days of storage.

Studies on the Yeasts for the Brewing of Soy sauce(Part 8) - Nutritional requirements of Saccharomyces rouxii T-9 (3) Influence of addition of vitamins and amino acids - (간장 발효에 관여하는 효모에 관한 연구 (제8보) - Saccharomyces rouxii T-9의 영양 요구성 (3) Vitamin 및 Amino acid의 영향에 대하여 -)

  • Lee, Taik-Soo;Lee, Suk-Kun
    • Applied Biological Chemistry
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    • v.15 no.1
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    • pp.59-63
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    • 1972
  • These experiment were conducted to study the effects of various vitamins and amino acids upon the growth of Saccharomyces rouxii T9 and the results obtained were as follows. (1) As a growth factor, inositol, ribofavin, niacin and para amino benzoic acid were not required. (2) Though biotin, thiamin and Ca-pantothenate were adaptably required, vitamins required in absolute were not existed. (3) The growth rate of Saccharomyces rouxii T9 in the deficient group of biotin, thiamin, pyridoxine, riboflavin and Ca-pantothenate were more decreased on the media containing 26% of NaCl than containing none of NaCl in the earlystage of cultivation, while the growth rate showed the opposition tendency in the latter term of cultivation. (4) As an amino acid, methionine, tryptophan, serine, threonine, cystine, glycine, leucine and valine showed systematically the effects of addition in the compound media containing $(NH_4)_2SO_4$ as a nitrogen sources. (5) The growth rate of Saccharomyces rouxii T9 in the addition group of histidine, lycine, arginine, aspartic acid, proline and tyrosine were somewhat decreased on the media containing higher concentration of NaCl comparison with none NaCl media in the early stage of cultivation, showing the similar or increasing tendency in the latter term of cultivation.

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Changes in Components of Salted Eggplants (Chukyang) during Storage (축양품종 염절임 가지의 저장중의 성분 변화)

  • Nam, Hak-Sik;Kim, Nam-Woo;Shin, Seung-Ryeul
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.1
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    • pp.120-125
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    • 2005
  • This study was carried out to investigate the changes in components of salted eggplant (chukyang) during storage. The contents of total and reducing sugar were decreased during storage, and the tendency was lower in the salted eggplants of vacuum packing storage than in the salted eggplants of rice bran immersion storage. The organic acids of salted eggplants were acetic acid, citric acid, lactic acid, and malic acid. And acetic and malic acid contents were much higher than the others. The content of acetic acid was increased during storage, but malic acid was decreased. Major amino acids of eggplants were valine, aspartic acid, glutamic acid, glycine, and alanine. The content of total amino acid was 1,488.18 mg/100 g in eggplant packed vacuum film at 20 days of storage, and higher than those in fresh eggplant. The content of total amino acid in eggplant immersed in wet rice bran was 745.42 mg/100 g at 20 days of storage, and decreased during storage. The contents of aspartic acid, alanine, cystine, and proline in free amino acids of salted eggplants were higher than other amino acids. The contents of phosphoserine, taurine, ${\gamma}$ -aminoisobutyric acid and hydroxyproline were higher than others. Posphoserine content was decreased during storage, but ${\gamma}$ -aminoisobutyric acid content was increased during storage.

Chemical Components of Propolis and Its Ethanolic Extracts (프로폴리스 및 알콜 추출물의 화학성분)

  • 정창호;배영일;이호재;심기환
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.4
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    • pp.501-505
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    • 2003
  • In order to use as a new functional food material, chemical components of propolis and its extracts were surveyed. The contents of crude fat, nitrogen free extract, crude protein, ash and crude fiber in propolis were 86.41%, 7.32%, 2.71%, 1.05% and 0.20%, respectively. The mineral contents were in the order of Na (120.40 mg%), Ca (115.40 mg%), K (105.87 mg%) and Ca were higher in water extract than alcohol extract. Free sugars were composed of sucrose 152 mg%, glucose 114 mg% and fructose 6 mg%. The major amino acids of propolis were lysine 395.29 mg%, cystine 267.66 mg% and glutamic acid 248.14 mg%, respectively. Eight fatty acids in propolis were identified and the major fatty acids were oleic acid (51.89%), myristic acid (20.86%) and palmitic acid (20.28%). Myricetin, quercetin, apigenin and kaempferol were shown as major flavonols and total flavonol contents were higher in 50% ethanol extract than any other extracts. Major Polyphenol compounds in four kinds of extracts were gallic acid, chlorogenic acid, catechin, epigallocatechin gallate, epicatechin and epicatechin gallate.

Re-evaluation of Dietary Methionine Requirement by Plasma Methionine and Ammonia Concentrations in Surgically Modified Rainbow Trout, Oncorhynchus mykiss

  • Bae, Jun-Young;Ok, Im-Ho;Lee, Seung-Hyung;Hung, Silas S.O.;Min, Tae-Sun;Bai, Sung-Chul C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.7
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    • pp.974-981
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    • 2011
  • This study was designed to re-evaluate the dietary methionine requirement by means of the plasma methionine and ammonia concentrations in surgically modified rainbow trout, Oncorhynchus mykiss. A total of 35 rainbow trout averaging $505{\pm}6.5$ g (initial body weight, mean${\pm}$SD) were randomly distributed into seven groups with five fish in each group. After 48 h of feed deprivation, each group of fish was fed one of seven L-amino acid based diets containing 0.5% cystine and graded levels of methionine (0.25, 0.40, 0.50, 0.60, 0.70, 0.80 or 0.95% of diet, dry matter bases) by intubation at 1% body weight on dry matter basis. Blood samples were taken at 0, 5 and 24 h after intubation. Post-prandial plasma free methionine concentrations (PPmet, 5 h after intubation) and post-absorptive plasma free methionine concentrations (PAmet, 24 h after intubation) of fish fed diets containing 0.60% or higher methionine were significantly (p<0.05) higher than those of fish fed diets containing 0.50% or lower methionine. PPmet and PAmet in fish fed diets containing 0.60% or higher methionine were not significantly different except PPmet of fish fed diet containing 0.95% methionine. Post-prandial plasma ammonia concentrations (PPA, 5 h after intubation) of fish fed diets containing 0.70% or higher methionine were significantly higher than those of fish fed diets containing 0.60% or lower methionine, and PPA of fish fed diets containing 0.25 and up to 0.60% methionine were not significantly different from each other. Broken-line model analyses on PPmet, PAmet, and PPA indicated that the dietary methionine requirement of rainbow trout was between 0.59 (1.69) and 0.67 (1.91) % of diets (% dietary protein bases) when the diets contained 0.5% cystine.