• Title/Summary/Keyword: curcumin content

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Effect of salicylic acid and yeast extract on curcuminoids biosynthesis gene expression and curcumin accumulation in cells of Curcuma zedoaria

  • Lan, Truong Thi Phuong;Huy, Nguyen Duc;Luong, Nguyen Ngoc;Quang, Hoang Tan;Tan, Trinh Huu;Thu, Le Thi Anh;Huy, Nguyen Xuan;Loc, Nguyen Hoang
    • Journal of Plant Biotechnology
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    • v.46 no.3
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    • pp.172-179
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    • 2019
  • The aim of this study is to evaluate the effect of yeast extract (YE) and salicylic acid (SA) on the expression of curcuminoid-biosynthesis genes (CzDCS and CURS1-3), and accumulation of curcumin in Curcuma zedoaria cell cultures. The results showed that, in cells treated with YE or SA, the expression levels of curcuminoid genes were 1.14- to 3.64-fold higher than the control (untreated cells), in which the YE exhibited a stronger effect in comparison with SA. Curcumin accumulation also tended to be similar to gene expression, curcumin contents in YE- or SA-treated cells were 1.61- to 2.53-fold higher than the control. The SA treatment at the fifth day of culture stimulated the curcumin accumulation and expression in all four genes compared to that at the beginning. While the YE treatments gave different results, the CzCURS1 and CzCURS3 genes were expressed strongly in cells that were treated at the beginning. However, the CzDCS and CzCURS2 genes showed the opposite expression pattern, they were activated strongly in the treatments at day five of the culture. However, the content of curcumin reached its maximum value on the fifth day of culture in all investigations.

Quality Characteristics of Low-Fat Sausage Containing Curcumin Extract during Cold Storage (울금 추출물 함유 저지방 소시지의 냉장저장 중 품질 특성)

  • Kim, Il-Suk;Jin, Sang-Keun;Park, Ki-Hoon;Jeong, Ki-Jong;Kim, Dong-Hoon;Yang, Mi-Ra;Chung, Young-Sin
    • Food Science of Animal Resources
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    • v.27 no.3
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    • pp.255-261
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    • 2007
  • The effects of curcumin extract on the physicochemical, microbial and sensory properties of low-fat sausages during refrigerated storage were studied. Sausage products were produced with three different formulations including 0%, 2.5%, and 5.0% curcumin extract. Low-fat sausages made with the addition of curcumin extract had lower (p<0.05) crude fat content, pH and TBARS values than the control sample. The addition of curcumin extract did not affect the water holding capacity, cooking loss, shear force, meat color, texture profile, and total bacterial count in low-fat sausages duringstorage (p>0.05). With regard to sensory evaluation, 2.5% curcumin extract added to low-fat sausages resulted in a high overall acceptability (p<0.05). In conclusion, low-fat sausages with added 2.5% curcumin extract had a higher acceptability and lipid oxidative stability during storage than products without curcumin extract.

Effects of Composts on the Growth, Yield and Effective Components of Turmeric (Curcuma longa L.) (울금의 생육, 수량 및 품질에 미치는 퇴비의 효과)

  • Han, Hyo-Shim;Woo, Seo;Kim, Dong-Kwan;Heo, Buk-Gu;Lee, Kyung-Dong
    • Korean Journal of Environmental Agriculture
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    • v.29 no.2
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    • pp.138-145
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    • 2010
  • Curcuma longa L. (turmeric) is an important medicinal plant that has been historically used in herbal medicine and in the health food throughout Asia etc. Recently, the demands on rhizome of turmeric are increasing greatly by well-being boom, but there is not enough to meet the demands. To fulfill increasing demands, cultivation system strategies using the organic fertilizers are required to produce a greater amount of rhizome with good quality and yield. A field experiment was conducted to determine the effect of composts, NPK fertilizer(control, F), NPK + swine manure(SM) and NPK + fermentation manure from the wasted oriental medicine materials(OMWM), on rhizome yield and the content of bioactive components for quality. Our results showed that two compost applications can increase both rhizomes(24.1-25.9%) and curcumin(21.7-41.0%) yields, respectively, compared to F control. The content of amino acids increased significantly by SM and OMWM treatments. SM and OMWM application also increased the total phenol yields 7.8 and 8.7 g/10a compared with control 6.3 g/10a, the flavonoid yields 6.3 and 7.3 g/10a compared with control 5.3 g/10a, and also antioxidant activity 21.7 and 41%, respectively, as compared to the control. Especially, OMWM was more effective in total rhizomes yields and bioactivities and in the biosynthesis of curcumin and bioactive components than SM treatments, but the biological pathway was not clear, still. This experiment suggests that curcumin or bioactive components affected by adding SM and OMWM could increase the yields and quality of turmeric.

Effects of Dietary Turmeric Powder on Laying Performance and Egg Qualities in Laying Hens

  • Park, Sang-Sul;Kim, Jin-Man;Kim, Eun-Jib;Kim, Hee-Sung;An, Byoung-Ki;Kang, Chang-Won
    • Korean Journal of Poultry Science
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    • v.39 no.1
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    • pp.27-32
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    • 2012
  • This experiment was conducted to investigate the dietary effects of turmeric powder (TP) on laying performances, egg qualities and its transfer into eggs. A total of two hundred, 60-wk-old, Lohmann Brown layers were divided into 4 groups, placed in to 5 replicates per group (10 layers each) and fed each one of four diets containing 0% TP (control), 0.10, 0.25 or 0.50% TP, respectively, for 7 wks. Egg production in the all groups fed diets containing TP were significantly higher than that in control (P<0.05). No differences in feed intakes, egg and eggshell qualities were observed among the treatments, but Roche color fan number (yolk color) in group fed diet with 0.5% TP was significantly higher than in control (P<0.05). In the groups fed diets containing TP, Haugh units after 2 wk of storage were significantly higher than that of control (P<0.05). The curcumin content of egg yolk in the groups fed diet containing 0.50% TP was significantly higher than those in the other groups (P<0.05). The cholesterol levels of egg yolk were not influenced by dietary TP. It was concluded that dietary TP was effective in improving laying performance and internal egg qualities. TP can be also used as a feed additive for the production of value-enhanced eggs with increased yolk color and curcumin content.

산란계 사료 내 강황분말(Turmeric powder)의 첨가 급여가 계란 생산성과 품질에 미치는 영향

  • Park, Sang-Seol;Yu, Seon-Jong;Kim, Jae-Yeong;Lee, Bo-Geun;Yun, Ji-Yeon;An, Byeong-Gi;Gang, Chang-Won
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2006.11a
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    • pp.62-63
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    • 2006
  • This experiment was conducted to investigate the dietary effect of turmeric powder (TP) on laying performances, egg qualities and its transfer to eggs. A total of two hundred, 60-wk-old, Lohmann Brown layers were divided into 4 groups, placed in to 5 replicates per group (10 layers each) and fed each one of four diets containing 0 % TP(control), 0.1 % TP, 0.25 % TP, or 0.5 % TP for 7 wks, respectively. Egg production and egg mass in groups fed diets containing TP were higher than that of control(p<0.05). No differences in feed intakes, egg and eggshell qualities were observed among the treatments, but egg yolk color was increased significantly as TP was in increased in the diet(p<0.05). In groups fed diets containing TP, Haugh unit after 14 day of storage was significantly higher than that of control(p<0.05). The curcumin content of egg yolk in groups fed diet containing 0.50% TP was significantly higher than those of other groups (p<0.05). The content of egg yolk cholesterol was not influenced by dietary TP.

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Curcumin Content of Cultivated Tumeric in Korea (재배(栽培) 울금(鬱金)의 쿨쿠민 함량(含量))

  • Chi, Hyung-Joon;Kim, Hyun-Soo
    • Korean Journal of Pharmacognosy
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    • v.14 no.2
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    • pp.67-69
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    • 1983
  • Tumeric, Curcuma longa L.(Zingiberaceae) is native to the southern Asia, cultivated mainly in India and has been used for the treatment of hemorrhages in oriental medicine. The ground powder has also been used for flavouring and dyeing agents. In this report, the results of trial cultivation of tumeric in the middle portion of Korea (Kyungi, Shihung) are summarized. The rhizomes used for this trial cultivation were purchased from the Takii Seed Co. (Japan) and planted in the late of April and harvested in the middle of November. The curcumin contents of harvested tumeric were about 0.1% and 0.24% from finger type and bulb type respectively and these are comparable with those of commercial tumerics.

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Combined Effects of Curcumin and (-)-Epigallocatechin Gallate on Inhibition of N-Acylhomoserine Lactone-Mediated Biofilm Formation in Wastewater Bacteria from Membrane Bioreactor

  • Lade, Harshad;Paul, Diby;Kweon, Ji Hyang
    • Journal of Microbiology and Biotechnology
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    • v.25 no.11
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    • pp.1908-1919
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    • 2015
  • This work investigated the potential of curcumin (CCM) and (-)-epigallocatechin gallate (EGCG) to inhibit N-acyl homoserine lactone (AHL)-mediated biofilm formation in gram-negative bacteria from membrane bioreactor (MBR) activated sludge. The minimum inhibitory concentrations (MICs) of CCM alone against all the tested bacteria were 200-350 μg/ml, whereas those for EGCG were 300-600 μg/ml. Biofilm formation at one-half MICs indicated that CCM and EGCG alone respectively inhibited 52-68% and 59-78% of biofilm formation among all the tested bacteria. However, their combination resulted in 95-99% of biofilm reduction. Quorum sensing inhibition (QSI) assay with known biosensor strains demonstrated that CCM inhibited the expression of C4 and C6 homoserine lactones (HSLs)-mediated phenotypes, whereas EGCG inhibited C4, C6, and C10 HSLs-based phenotypes. The Center for Disease Control biofilm reactor containing a multispecies culture of nine bacteria with one-half MIC of CCM (150 μg/ml) and EGCG (275 μg/ml) showed 17 and 14 μg/cm2 of extracellular polymeric substances (EPS) on polyvinylidene fluoride membrane surface, whereas their combination (100 μg/ml of each) exhibited much lower EPS content (3 μg/cm2). Confocal laser scanning microscopy observations also illustrated that the combination of compounds tremendously reduced the biofilm thickness. The combined effect of CCM with EGCG clearly reveals for the first time the enhanced inhibition of AHL-mediated biofilm formation in bacteria from activated sludge. Thus, such combined natural QSI approach could be used for the inhibition of membrane biofouling in MBRs treating wastewaters.

Effects of Ethanol Extracts from Zingiber officinale Rosc., Curcuma longa L., and Curcuma aromatica Salisb. on Acetylcholinesterase and Antioxidant Activities as well as GABA Contents (생강, 울금, 강황 추출물의 항산화 효과, AChE 억제활성 및 GABA 함량)

  • Jung, Yeon-Seop;Park, Sung-Jin;Park, Jung-Hyun;Jhee, Kwang-Hwan;Lee, In-Seon;Yang, Seun-Ah
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.10
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    • pp.1395-1401
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    • 2012
  • This study investigated the cognition-related effects on antioxidant activities, ${\gamma}$-aminobutyric acid (GABA) contents, and AChE inhibitory activities in ethanol extracts from Zingiber officinale Rosc. (Korea), Curcuma longa L. (Korea), Curcuma longa L. (Myanmar), and Curcuma aromatica Salisb. (India). These extracts were investigated to determine the relationships among total polyphenols as well as DPPH and ABTS radical scavenging activities, GABA contents, and acetylcholinestrase (AChE) inhibitory activity. For the results, C. longa L. from Myanmar exhibited the highest contents of curcumin (97.1 ${\mu}g/mg$), total polyphenols (98.4 ${\mu}g/mg$), and GABA (1.31 ${\mu}g/mg$), as well as the strongest radical scavenging activities and AChE inhibitory activity. In addition, C. aromatica Salisb from India, which had the highest total polyphenol content (98.4 ${\mu}g/mg$) and strongest radical scavenging activities, exhibited relatively high AChE inhibitory activity similar to that of C. longa L. from Myanmar. On the other hand, Z. officinale Rosc. and C. longa L. from Korea showed low contents of curcumin (12.2 ${\mu}g/mg$) and polyphenols (85.7 ${\mu}g/mg$), as well as low AChE inhibitory activities. However, we could detect 1.11 ${\mu}g/mg$ of GABA in these extracts, which was similar to that of C. longa L. from Myanmar. Therefore, GABA content was not correlated with AChE inhibitory activity. Based on the results, AChE inhibitory activity is highly correlated with polyphenol contents in Zingibearceae family. Overall, among the Zingiberaceae tested, C. longa L. from Myanmar might be a strong candidate as a cognitive-enhancing ingredient.

Quantitative Determination and Stability of Curcuminoid Pigments from Turmeric (Curcuma longa L.) Root (강황 (Curcuma longa L.) 색소의 정량 및 안정성)

  • Kim Kwan-Su;Choung Myoung-Gun;Park Si-Hyung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.spc1
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    • pp.211-215
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    • 2005
  • Three curcuminoids [curcumin (CUR), demethoxycurcumin(DEM), bisdemethoxycurcu in (BIS)] are major yellow pigments in turmleric (Curcuma longa L.) root. Contents of curcuminoids in turmeric roots collected from 6 locations were analyzed using, high performance liquid chromatography (HPLC) equipped with reversed-phase column, an UV-Vis detector at 420nm, and eluted with a mixture of acetonitrile: $0.1\%$ acetic acid in water (50 : 50, v/v) as mobile phase. The stability of curcuminoid pigments in $80\%$ methanol extract solution were investigated during storage in a freezer at $-20^{\circ}C$, room temperature in the dark, and light condition. Calibration curves for the determination of curcuminoids were made with significant linearity $(r^2=0.999**)$. Average content of total curcuminoids was 171.5 mg/g, with 91.6 mg/g of CUR, 56.9 mg/g of DEM, and 23.0 mg/g of BIS. Amount of curcuminoids during storage in a freezer was almost not changed while those in room temperature wert reduced and rapid degradation appeared after 60 days. Within 90 days, about $50\%$ curcuminoid decreased in the dark and about $70\%$ in the light condition, indicating the decomposition of curcuminoid pigments followed under light and heat.