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http://dx.doi.org/10.3746/jkfn.2012.41.10.1395

Effects of Ethanol Extracts from Zingiber officinale Rosc., Curcuma longa L., and Curcuma aromatica Salisb. on Acetylcholinesterase and Antioxidant Activities as well as GABA Contents  

Jung, Yeon-Seop (Dept. of Food Science and Technology, Keimyung University)
Park, Sung-Jin (Dept. of Food Science and Technology, Keimyung University)
Park, Jung-Hyun (The Center for Traditional Microorganism Resources, Keimyung University)
Jhee, Kwang-Hwan (Dept. of Applied Chemistry, Kumoh National Institute of Technology)
Lee, In-Seon (Dept. of Food Science and Technology, Keimyung University)
Yang, Seun-Ah (The Center for Traditional Microorganism Resources, Keimyung University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.41, no.10, 2012 , pp. 1395-1401 More about this Journal
Abstract
This study investigated the cognition-related effects on antioxidant activities, ${\gamma}$-aminobutyric acid (GABA) contents, and AChE inhibitory activities in ethanol extracts from Zingiber officinale Rosc. (Korea), Curcuma longa L. (Korea), Curcuma longa L. (Myanmar), and Curcuma aromatica Salisb. (India). These extracts were investigated to determine the relationships among total polyphenols as well as DPPH and ABTS radical scavenging activities, GABA contents, and acetylcholinestrase (AChE) inhibitory activity. For the results, C. longa L. from Myanmar exhibited the highest contents of curcumin (97.1 ${\mu}g/mg$), total polyphenols (98.4 ${\mu}g/mg$), and GABA (1.31 ${\mu}g/mg$), as well as the strongest radical scavenging activities and AChE inhibitory activity. In addition, C. aromatica Salisb from India, which had the highest total polyphenol content (98.4 ${\mu}g/mg$) and strongest radical scavenging activities, exhibited relatively high AChE inhibitory activity similar to that of C. longa L. from Myanmar. On the other hand, Z. officinale Rosc. and C. longa L. from Korea showed low contents of curcumin (12.2 ${\mu}g/mg$) and polyphenols (85.7 ${\mu}g/mg$), as well as low AChE inhibitory activities. However, we could detect 1.11 ${\mu}g/mg$ of GABA in these extracts, which was similar to that of C. longa L. from Myanmar. Therefore, GABA content was not correlated with AChE inhibitory activity. Based on the results, AChE inhibitory activity is highly correlated with polyphenol contents in Zingibearceae family. Overall, among the Zingiberaceae tested, C. longa L. from Myanmar might be a strong candidate as a cognitive-enhancing ingredient.
Keywords
Zingiberaceae; antioxidant; gamma-aminobutyric acid; acetylcholinesterase; Curcuma aromatica Salisb;
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Times Cited By KSCI : 9  (Citation Analysis)
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1 Kim CR. 2006. Enhancement of liver function by Curcuma extract on acute hepatotoxicity in rat. Korean J Food Sci Ani Resour 26: 386-393.   과학기술학회마을
2 Jeong SH, CHang KS, Kim YJ. 2004. Optimization of curcumin extraction from turmeric (Curcuma longa L.) using supercritical fluid $CO_{2}$. Food Engineering Progress 8: 47-52.
3 Jung SH, Chang KS, Ko GH. 2004. Physiological effects of curcumin extracted by supercritical fluid from turmeric (Curcuma longa L.). Korean J Food Sci Technol 36: 317- 320.   과학기술학회마을
4 Kim JS, Kim YS, Kim SK, Heor JH, Lee BH, Choi BW, Ryu GS, Park EK, Zee OP, Ryu SY. 2002. Inhibitory effects of some herbal extracts on the acetylcholinesterase (AChE) in vitro. Korean J Pharmacogn 33: 211-218.   과학기술학회마을
5 Folin O, Denis W. 1912. Phosphotungastic-phospho-molybdic compounds as color reagents. J Biol Chem 12: 239-249.
6 Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.   DOI   ScienceOn
7 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237.   DOI   ScienceOn
8 Zhang G, Bown AW. 1996. The rapid determination of $\gamma$- aminobutyric acid. Phytochemistry 44: 1007-1009.
9 Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7: 88-90.   DOI   ScienceOn
10 Sandahl JF, Jenkins JJ. 2002. Pacific steelhead (Oncorhynchus mykiss) exposed to chlorpyrifos: benchmark concentration estimates for acetylcholinesterase inhibition. Environ Toxicol Chem 21: 2452-2458.   DOI
11 Lee TY, Lee KC, Chen SY, Chang HH. 2009. 6-Gingerol inhibits ROS and iNOS through the suppression of PKC-$\alpha$ and NF-$\kappa B$ pathways in lipopolysaccharide-stimulated mouse macrophages. Biochem Biophys Res Commun 382: 134-139.   DOI
12 Dugasani S, Pichika MR, Nadarajah VD, Balijepalli MK, Tandra S, Korlakunta JN. 2010. Comparative antioxidant and anti-inflammatory effects of [6]-gingerol, [8]-gingerol, [10]-gingerol and [6]-shogaol. J Ethnopharmacol 127: 515-520.   DOI
13 Wu H, Hsieh MC, Lo CY, Liu CB, Sang S, Ho CT, Pan MH. 2010. 6-Shogaol is more effective than 6-gingerol and curcumin in inhibiting 12-O-tetradecanoylphorbol 13-acetateinduced tumor promotion in mice. Mol Nutr Food Res 54: 1296-12306.   DOI
14 Jeong SH, Chang KS, Kim YJ. 2004. Optimization of curcumin extraction from turmeric (Curcuma longa L.) using supercritical fluid $CO_{2}$. Food Eng Progress 8: 47-52.
15 Choi HS. 1994. Lipid peroxidation and its nutritional significance. J Korean Soc Food Nutr 23: 867-878.   과학기술학회마을
16 Pratt DE. 1992. Natural antioxidant from plant material. In Phenolic Compounds in Food and Their Effects on Health. American Chemical Society, Washington, DC, USA. p 54-71.
17 Higasi GS. 2000. Appraisement of antioxidative activity from vegetables. Jap J Food Ind 57: 56-64.
18 Goel A, Kunnumakkara AB, Aggarwal BB. 2008. Curcumin as "Curecumin": from kitchen to clinic. Biochem Pharmacol 75: 787-809.   DOI   ScienceOn
19 Floyd RA, Hensley K. 2002. Oxidative stress in brain aging. Implications for therapeutics of neurodegenerative diseases. Neurobiol Aging 23: 795-807.   DOI
20 Montine TJ, Neely MD, Quinn JF, Beal MF, Markesbery WR, Roberts LJ, Morrow JD. 2002. Lipid peroxidation in aging brain and Alzhemier's disease. Free Radic Biol Med 33: 620-626.   DOI   ScienceOn
21 Al-sereiti MR, Abu-Amer KM, Sen P. 1999. Pharmacology of rosemary (Rosmarinus officinalis Linn) and its therapeutic potentials. Indian J Exp Biol 37: 124-130.
22 Kang YH, Park YK, Oh SR, Moon KD. 1995. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J Food Sci Technol 27: 978-984.   과학기술학회마을
23 Lee SO, Lee HJ, Yu MH, Im HG, Lee IS. 2005. Total polyphenol contents and antioxidant activities of methanol extract from vegetable produced in Ullung isand. Korean J Food Sci Technol 37: 233-240.
24 Choi YM, Kim MH, Shin JJ, Park JM, Lee JS. 2003. The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr 32: 723-727.   과학기술학회마을   DOI
25 Bown AW, Shelp BJ. 1997. The metabolism and function of $\gamma$-aminobutyric acid. Plant Physiol 115: 1-5.   DOI
26 DiFiglia M, Aronin N. 1990. Synaptic interactions between GABAergic neurons and trigeminothalamic cell in the rat trigeminal nucleus caudalis. Synapse 6: 358-363.   DOI
27 Ryu BH, Jeon JH. 2004. Continous production of $\gamma$-aminobutyric acid by immobilization of Lactobacillus brevis. J Life Sci 14: 167-173.   DOI   ScienceOn
28 Oh SK. 2005. Neurotransmetters and Brain Disease. Shinil Books company, Seoul, Korea. p 345-364.
29 Talesa VN. 2001. Acetylcholinesterase in Alzheimer's disease. Mech Ageing Dev 122: 1961-1969.   DOI   ScienceOn
30 Trabace L, Cassano T, Steardo L, Pietra C, Villetti G, Kendrick KM, Cuomo V. 2000. Biochemical and neurobehavioral profile of CHF2819, a novel, orally active acetylcholinesterase inhibitor for Alzheimer's disease. J Pharmacol Exp Ther 294: 187-194.
31 Park CH, Kim SH, Choi W, Lee YJ, Kim JS, Kang SS, Suh YH. 1996. Novel anticholinesterase and antiamnesic activities of dehydroevodiamine, a constituent of Evodia rutaecarpa. Planta Med 62: 405-409.   DOI   ScienceOn
32 Chung YK, Heo HJ, Kim EK, Kim HK, Huh TL, Lim Y, Kim SK, Shin DH. 2001. Inhibitory effect of ursolic acid purified from Oiganum majorana L on the acetylcholinesterase. Mol Cells 11: 137-143.
33 Papandreou MA, Dimakopoulou A, Linardaki ZI, Cordopatis P, Klimis-Zacas D, Margarity M, Lamari FN. 2009. Effect of a polyphenol-rich wild blueberry extract on cognitive performance of mice, brain antioxidant markers and acetylcholinesterase activity. Behav Brain Res 198: 352-358.   DOI
34 Amenta F, Parnetti L, Gallai V, Wallin A. 2001. Treatment of cognitive dysfunction associated with Alzheimer's disease with cholinergic precursors. Ineffective treatments or inappropriate approaches? Mech Ageing Dev 122: 2025-2040.   DOI   ScienceOn
35 Fayuk D, Yakel JL. 2004. Regulation of nicotinic acetylcholine receptor channel function by acetylcholinesterase inhibitors in rat hippocampal CA1 interneurons. Mol Pharmacol 66: 658-666.   DOI
36 Lim SD, Kim KS. 2009. Effects and utilization of GABA. Korean J Dairy Sci Technol 27: 45-51.   과학기술학회마을
37 Liu Q, Zhao B. 2004. Nicotine attenuates $\beta$-amyloid peptide- induced neurotocity, free radical and calcium accumulation in hippocampal neuronal cultures. Br J Pharmacol 141: 746-754.   DOI
38 Oh SH, Kim SH, Moon YJ, Choi WG. 2002. Changes in the levels of $\gamma$-aminobutyric acid and some amino acids by application of a glutamic acid solution for the germination of brown rices. Korea J Biotechnol Bioeng 17: 49-53.
39 Xinga SG, Jun YB, Hau ZW, Liang LY. 2007. Higher accumulation of $\gamma$-aminobutyric acid induced by salt stress through stimulating the activity of diamine oxidases in Glycine max (L.) Merr. roots. Plant Physiol Biochem 45: 560-566.   DOI
40 Shelp BJ, Bown AW, McLean MD. 1999. Metabolism and functions of gamma-aminobutyric acid. Trends Plant Sci 4: 446-452.   DOI   ScienceOn
41 Ahn DG. 2000. Korean herbal flora. Kyohak Publishing Co., Seoul, Korea. p 568-569.
42 Huang MT, Lou YR, Xie JG, Ma W, Lu YP, Yen P, Zhu BT, Newmark H, Ho CT. 1998. Effect of dietary curcumin and dibenzoylmethane on formation of 7,12-dimethylbenz [${\alpha}$]anthracene-induced mammary tumors and lymphomas/ leukemias in Sencar mice. Carcinogenesis 19: 1697-1700.   DOI   ScienceOn
43 Chainani-Wu N. 2003. Safety and anti-inflammatory activity of curcumin: a component of turmeric (Curcuma longa). J Altern Complement Med 9: 161-168.   DOI
44 Kang WS, Kim JH, Park EJ, Yoon KR. 1998. Antioxidative property of turmeric (Curcumae Rhizoma) ethanol extract. Korean J Food Sci Technol 30: 266-271.   과학기술학회마을