• 한국작물학회지
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• 제7권1호
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• pp.161-165
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• 1969

Since Guha and Maheshwari's works on the induction of haploid plant from the cultured anther of Datura innoxia an her culture has become a big topic among geneticists and breeders. Presen paper is the summarized report on the anther culture method based on the author's recent researches.

• 한국콘텐츠학회논문지
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• 제11권6호
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• pp.438-448
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• 2011

본 연구는 조건부가상가치법(CVM)을 이용하여 스토리텔링 지원사업에 대한 경제적 가치를 추정함으로써 스토리텔링 육성의 필요성과 이를 활용한 문화 관광산업 활성화 방안 수립 및 정책 시행에 시사점을 제시하는 데 그 목적이 있다. 로짓모형과 WTP 평균치를 이용하여 지불의사액을 추정한 결과 스토리텔링 활성화 사업에 대한 경제적 가치는 1인당 연간 약 1만6천원으로 나타났다. 문화콘텐츠에 대한 지출액과 스토리텔링 활성화 정책에 대한 인지도 및 지지도, 그리고 지불의사액이 정책지원에 대한 참여의사에 유의한 영향을 미치는 것으로 나타났다. 이러한 분석결과는 향후 문화콘텐츠에 대한 관심과 소비가 증가함에 따라 스토리텔링 활성화에 대한 경제적 가치는 더욱 증가할 것이며, 스토리텔링 육성에 대한 정책 및 사업을 국민들에게 알리고 지지를 얻도록 소통하는 것도 스토리텔링 육성의 경제적 가치를 높이는데 필요한 부분이라는 점을 시사하고 있다.

• 기록학연구
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• 제24호
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• pp.173-214
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• 2010

문화자원 기록화에 대한 의미와 필요성에 대한 사회적 인식이 높아지고 있다. 문화자원 중에서도 무형 문화재(無形文化財)는 재현하는 사람이 없거나, 무형문화재 관련 기록이 남아있지 않으면 더 이상 재현이 불가능하다는 점에서 기록화가 무엇보다 중요하다. 이러한 문제의식에서 시작된 본 연구는 강릉단오제의 기록화 방법을 제시하고자 하였다. 본 연구에서는 무형문화재가 생성, 유지되고, 보존되는 생애 전 과정을 고려한 기록화 방안을 설계하고자 하였다. 강릉단오제를 기록화 하기 위한 방안으로 기록화 대상을 지정하였으며, 기록화 대상은 강릉단오제의 본자원과 보조자원으로 구분하였다. 강릉단오제의 본자원과 보조자원의 대상에 대해 업무 프로세스를 세워 업무 행위마다 발생되는 기록과 그 기록을 생산하는 주체, 기록이 생산되는 지점을 파악하였다. 이러한 분석을 통해 도출된 기록화 대상에 대해서는 기록화 방법을 제시하였고, 기록화 주체를 제안하였다.

• 한국식생활문화학회지
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• 제25권4호
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• pp.400-409
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• 2010

This study investigated the food and dietary educational content in primary, middle and high school textbooks in order to provide fundamental data for the development of educational programs on Korean traditional food culture. The research objects consisted of 51 kinds of textbooks (15 kinds of primary school textbooks, 29 kinds of middle school home economics textbooks and 7 kinds of high school home economics textbooks), and the contents related to food and dietary education were counted and analyzed. The content analysis was performed using two categories: application method and subject matter. Application method included texts, cases, visual aids (pictures, photos, illustrations, chart, etc.) and activities, whereas subject matter consisted of seven types (well balanced nutrition and health, understanding of food and nutrition, cooking principles, cooking lessons, traditional foods and culture, others). The results of the application method in primary school textbooks show that visual aids were the most common in all six grades. For the subject matter, 'understanding of food and nutrition' was most abundant in primary school textbooks while 'well balanced nutrition and health' accounted for a large part of the contents in middle school textbooks. However, the contents regarding traditional foods and culture were insufficient in primary and middle school textbooks. These results suggest that educational contents on traditional foods and culture should be added to primary and middle school textbooks and covered in various subjects. Furthermore, high school 'home economics' contents need to emphasize comprehensive food and dietary education and adjust to 'science & technology for life'.

• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.560-567
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• 2008

An efficient method to produce water-soluble polysaccharides from Lentinus lepideus is described. The productivity of both endopolysaccharides (PPS) and exopolysaccharides (EPS) was compared under various culture conditions. The effect of treating their own PPS and EPS on immune cytokine production was also studied in relation to culture factors. High yield production of EPS required a moderate culture temperature $(25^{\circ}C)$ as well as long culture period (16-20 days). In contrast, PPS production required a high culture temperature $(30^{\circ}C)$ and short culture period (8 days). Most of the carbon sources did not affect polysaccharides and mycelial production except for sucrose. Immune cytokine levels in the EPS treatment varied among carbon sources or culture periods. PPS did not appear to affect much on the production of cytokines, regardless of the culturing factors, except for the culture period. These results suggest that the optimal culture conditions for L. lepideus vary according to culture purposes, and different culture conditions should be used for different targets including mycelial biomass, EPS, and PPS. Whereas the immunomodulating activitiy of EPS appeared to be affected by culture conditions in L. lepideus, that of PPS did not.

• 간호행정학회지
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• 제17권1호
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• pp.88-95
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• 2011

Purpose: This study was done to identify the effect of nursing organizational culture on empowerment as perceived by new clinical nurses. Method: For the objective a sample of 175 new nurses from secondary/tertiary hospitals having not less than 250 beds located in B city was selected to complete a questionnaire. The collected data were analyzed using descriptive statistics, t-test, One-way ANOVA, Scheffe's test, Pearson correlation coefficients and simultaneous multiple regression with the SPSS 14.0 software program. Results: As for nursing organizational culture as perceived by the respondent, relation-oriented culture had the highest average score ($3.60{\pm}.63$) out of a possible 5 points followed by hierarch-oriented culture, innovation-oriented culture and task-oriented culture with $3.45{\pm}.30$, $3.19{\pm}.53$ and $3.05{\pm}.46$, respectively. The respondents' level of perceived empowerment was $3.06{\pm}.41$. The factors influencing empowerment according to nursing organizational culture were innovation-oriented and relation-oriented culture in that order. These 2 variables were statistically significant predictors and explained 48.6% of the variance in empowerment. Conclusions: The results of this study indicate that emphasis should be given to nursing organizational culture strengthened by innovationoriented and relation-oriented culture, rather than task-oriented and hierarch-oriented culture, in order to foster harmonious empowerment among nurses at all levels.

• 한국어교육
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• 제29권1호
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• pp.53-84
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• 2018

The main purpose of this study is to investigate foreigners' perception regarding Korean business culture and Korean business culture education. People who participated in this study are six foreign workers after studying at the university or graduate school in Korea. A phenomenological qualitative research method was used in this study in order to elicit life experiences from foreign workers who were working in Korea business companies. Therefore, their experience regarding Korean business culture and business culture education were measured by in-depth interviews. This study shows that nun-chi, vertical relationships, and impatience of Koreans are the most difficult culture to foreign workers. They also feel difficulties regarding Koreans' lack of understanding about other cultures. Moreover, these were not difficult components within school life; rather, the most difficult aspects were that they have never learned about the Korean business culture before getting a job. The results show that international students who want to work in Korea companies need to study about the culture of Korean business and revealed the desirability to learn. These results can be used as a foundation for understanding some contents and criterion of Korean business culture and education.

• Journal of Microbiology and Biotechnology
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• 제28권1호
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• pp.32-39
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• 2018

Samples of Laru (a fermentation starter) obtained from the upper part of Borneo Island were analyzed for their lactic acid bacteria (LAB) and fungal diversity using both a culture-independent method (PCR-DGGE) and culture-dependent methods (SDS-PAGE and MALDI-TOF MS). Pediococcus pentosaceus, Lactobacillus brevis, Saccharomycopsis fibuligera, Hyphopichia burtonii, and Kodamaea ohmeri were detected by all three methods. In addition, Weissella cibaria, Weissella paramesenteroides, Leuconostoc citreum, Leuconostoc mesenteroides, Lactococcus lactis, Rhizopus oryzae/Amylomyces rouxii, Mucor indicus, and Candida intermedia were detected by PCR-DGGE. In contrast, Lactobacillus fermentum, Lactobacillus plantarum, Pichia anomala, Candida parapsilosis, and Candida orthopsilosis were detected only by the culture-dependent methods. Our results indicate that the culture-independent method can be used to determine whether multiple laru samples originated from the same manufacturing region; however, using the culture-independent and the two culture-dependent approaches in combination provides a more comprehensive overview of the laru microbiota.

• Animal cells and systems
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• 제13권3호
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• pp.267-274
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• 2009

The dissociative culture technique of Aplysia neuron is one of the key methods that have been used for studies of cellular and molecular mechanisms of neuronal functioning. However, despite the advantages this method offers as an experimental model, its technical efficiency has had room for improvement. In this study, we examined certain putative factors that might affect the culture quality. The effects of neuronal damage induced by physical injuries, heat shock, and surface protein degradation were evaluated along with the correlation between the culture quality and animal behavior. As a result, we found that physical injury can be a critical factor that affects culture quality, whereas the heat shock and surface protein degradation had negligible effect on it. In addition, we discovered that siphon retraction time was not a good measurement for healthy neurons. Based on these findings, we suggest here an improved method in which the degree of physical injury is reduced by means of multiple protease treatment.

• 한국식물병리학회지
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• 제13권4호
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• pp.219-224
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• 1997

여러 계통의 PVY 외피단백질 유전자간에 상동성이 높은 부위에 위치한 primer 쌍을 이용하여 RT-PCR 방법으로 PVY 검정법을 개발하였는데 여러 line의 대서품종으로부터 764 bp의 PCR 산물이 합성되었고 이 절편을 sequencing한 결가 PVY의 유전자임을 확인하였다. 싹과 괴경 조직에서 RT-PCR에 의한 PVY의 검정의 민감도는 ELISA 방법보다 약 1,000배정도 높았다.